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The map label for this gene is gcvH

Identifier: 21243787

GI number: 21243787

Start: 3582825

End: 3583220

Strand: Reverse

Name: gcvH

Synonym: XAC3060

Alternate gene names: 21243787

Gene position: 3583220-3582825 (Counterclockwise)

Preceding gene: 21243788

Following gene: 21243786

Centisome position: 69.23

GC content: 62.37

Gene sequence:

>396_bases
ATGAGCGAGATCCCTGGCGACCTCAAGTTCCTCAAATCCCACGAGTGGGCCCGCGTCGAGAGCAACGGTCGCGTGACCGT
CGGCATTTCCGACCACGCGCAGGGCTTGCTGGGTGACCTGGTTTACGTCGAATTGCCAGGTGTCGGCGACACCGTGCAGG
TCGGCAACGGCGCTGCGGTGGTGGAATCGGTCAAGGCCGCCTCCGACGTCTACAGCCCGGTCAGCGGCACCGTCGTCGAG
GTCAACAGCGCGTTGAGCGACAAGCCGGAAACCATCAACGAAGACGCCTACGGCGAAGGCTGGATCTTCGTCGTCGAGAT
CGACGACAAGGAACAGCTCAACGACCTGTTGGACCCGGACGACTATGCCGAGCTGCTCGAAGACGACGATCACTGA

Upstream 100 bases:

>100_bases
TCCGGATCGCCATCGCCACACCGCCGTGCGTGCACCTGTCTTGTCGCGCGGTCTAAACTAGCCACCTGTCCCCAACCATC
ATCTTTCCCGGAGCACACCC

Downstream 100 bases:

>100_bases
TCAGGTGTCGTTGACGCCGGCATCCGATGGCCGCTCATAGAGCGGCCATTTTTTTGAGCACGCCTTCTGTCGGTGACGCG
TATCTTGGTGCAGCGATGCC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MSEIPGDLKFLKSHEWARVESNGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAVVESVKAASDVYSPVSGTVVE
VNSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPDDYAELLEDDDH

Sequences:

>Translated_131_residues
MSEIPGDLKFLKSHEWARVESNGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAVVESVKAASDVYSPVSGTVVE
VNSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPDDYAELLEDDDH
>Mature_130_residues
SEIPGDLKFLKSHEWARVESNGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAVVESVKAASDVYSPVSGTVVEV
NSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPDDYAELLEDDDH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=40.8333333333333, Blast_Score=100, Evalue=5e-22,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=40, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=55.8139534883721, Blast_Score=150, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=44.2622950819672, Blast_Score=106, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=121, Percent_Identity=41.3223140495868, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=132, Percent_Identity=37.1212121212121, Blast_Score=95, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17865652, Length=131, Percent_Identity=38.9312977099237, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_XANAC (Q8PI38)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643369.1
- ProteinModelPortal:   Q8PI38
- SMR:   Q8PI38
- GeneID:   1157131
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3060
- NMPDR:   fig|190486.1.peg.3013
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   XAXO190486:XAC3060-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14101; Mature: 13970

Theoretical pI: Translated: 3.74; Mature: 3.74

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIPGDLKFLKSHEWARVESNGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAV
CCCCCCHHHHHHCCCCEEECCCCEEEEEECHHCHHHHCCEEEEEECCCCCEEEECCCHHH
VESVKAASDVYSPVSGTVVEVNSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPD
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCC
DYAELLEDDDH
HHHHHHCCCCC
>Mature Secondary Structure 
SEIPGDLKFLKSHEWARVESNGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAV
CCCCCHHHHHHCCCCEEECCCCEEEEEECHHCHHHHCCEEEEEECCCCCEEEECCCHHH
VESVKAASDVYSPVSGTVVEVNSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPD
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCC
DYAELLEDDDH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217