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The map label for this gene is ptsN [H]

Identifier: 21243701

GI number: 21243701

Start: 3476102

End: 3476560

Strand: Direct

Name: ptsN [H]

Synonym: XAC2974

Alternate gene names: 21243701

Gene position: 3476102-3476560 (Clockwise)

Preceding gene: 21243700

Following gene: 21243702

Centisome position: 67.16

GC content: 69.93

Gene sequence:

>459_bases
ATGCCTTTAACCGATCTGATGGCGGCCGTGCGCACCCTGGTGTCGCCGGCCGCCGATCGCGACACCGTCCTGCATGCCGC
CGCCGATCTGCTCTCCTGTCGTCAGGCCGGCGCCGACGAGCTCTACGCCAACCTGTGCGAGCGCGAGGCCCTGGGCAGTA
CCGCCATTGGCCATGGCATCGCCATTCCGCATGGGCGCTGCCCCAACCTCACCGAGCCACGCGGGGCCTTGCTGCGGCTG
GACACGCCGGTGCCCTTCGGCAGCGACGAGCCGGTGGATCTGGTCTTCGCCATGGCCGTGCCGGCGCACTACACCCACCA
GCACCTGATGCTGCTGTCCGAGCTGGCCGAACGGTTCTCCGATGCCGACTTCCGTCGGAACTTGCGCACCGCACCCAACG
CCGATGCCCTGATGGCGCTGCTGGCCGACGTGCCGCCCGCGCAGGCGAGCGCGGCATGA

Upstream 100 bases:

>100_bases
GCTCGACCGCCTGGTCCTCAAGCACAAAGAGAAGAAATGTAACCACCACGCCCGCGAGGTGCGCGACAATCCCGCATGAC
GCTTCCCGCCTGATGCCCCC

Downstream 100 bases:

>100_bases
ATACCAGCATCACCGCACGCGAACTGTTCGACCAGCAGCGCGACAAGCTGGCCCTGCGCTGGGTCGCCGGCCCCAAGGGC
GAGCACCGCGAGATCCAGGC

Product: nitrogen regulatory IIA protein

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MPLTDLMAAVRTLVSPAADRDTVLHAAADLLSCRQAGADELYANLCEREALGSTAIGHGIAIPHGRCPNLTEPRGALLRL
DTPVPFGSDEPVDLVFAMAVPAHYTHQHLMLLSELAERFSDADFRRNLRTAPNADALMALLADVPPAQASAA

Sequences:

>Translated_152_residues
MPLTDLMAAVRTLVSPAADRDTVLHAAADLLSCRQAGADELYANLCEREALGSTAIGHGIAIPHGRCPNLTEPRGALLRL
DTPVPFGSDEPVDLVFAMAVPAHYTHQHLMLLSELAERFSDADFRRNLRTAPNADALMALLADVPPAQASAA
>Mature_151_residues
PLTDLMAAVRTLVSPAADRDTVLHAAADLLSCRQAGADELYANLCEREALGSTAIGHGIAIPHGRCPNLTEPRGALLRLD
TPVPFGSDEPVDLVFAMAVPAHYTHQHLMLLSELAERFSDADFRRNLRTAPNADALMALLADVPPAQASAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=106, Percent_Identity=40.5660377358491, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI48994992, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1786951, Length=140, Percent_Identity=31.4285714285714, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16161; Mature: 16029

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTDLMAAVRTLVSPAADRDTVLHAAADLLSCRQAGADELYANLCEREALGSTAIGHGI
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHCCCCC
AIPHGRCPNLTEPRGALLRLDTPVPFGSDEPVDLVFAMAVPAHYTHQHLMLLSELAERFS
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
DADFRRNLRTAPNADALMALLADVPPAQASAA
CHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PLTDLMAAVRTLVSPAADRDTVLHAAADLLSCRQAGADELYANLCEREALGSTAIGHGI
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHCCCCC
AIPHGRCPNLTEPRGALLRLDTPVPFGSDEPVDLVFAMAVPAHYTHQHLMLLSELAERFS
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
DADFRRNLRTAPNADALMALLADVPPAQASAA
CHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]