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The map label for this gene is potF [H]

Identifier: 21243209

GI number: 21243209

Start: 2882510

End: 2883625

Strand: Reverse

Name: potF [H]

Synonym: XAC2476

Alternate gene names: 21243209

Gene position: 2883625-2882510 (Counterclockwise)

Preceding gene: 21243210

Following gene: 21243208

Centisome position: 55.72

GC content: 61.65

Gene sequence:

>1116_bases
ATGACGCTGCGACTGCTCGCCCTGACGCTGTCCATCACCCTGTTGTCCGGCTGTGGCGGCGCTGGCGCGCCCGGCAGCGC
CGACGCGCAGGCCAAGGTGCTCAACGTCTACAACTATTCCGATTACATCGCCGAAGACACCATTCCCGCGTTCGAAAAGA
GCACCGGCATCAAGGTGACCTACGACGTCTTCGACAGCGACGAAATGGTGGAAACCAAGTTGCTGGCCGGCGGCAGCGAT
TACGACGTGGTGGTGCCCACGCTGAACTTCTTCGGCCGGCAGATCCAGGCCGGCGTCTTCCTGCCGCTGGACAAGAGCAA
GATCCCCAATCTGGTCAATCTGGATCCGGACGTGATGCGCCGCATCGCCGCGCAGGACCCGAACAACGCCTATGGCGTGC
CGTACATGATCGGCACCACTGGCATCGGCTACAACGTCGACAAGCTCAAGGCGATCTTCGGCAACACCGATGTCGCCAAC
AGCTGGGATCTGGTGTTCAAGCCGGAGAATCTGTCCAAACTCAAGGACTGTGGCGTGACCATCCTGGACACGCCTTCGGA
CATGATTCCCATCGCGCTCAATTATCTCGGGCTCGATCCGCACAGCACCACGCCGGCCGAGATCGAAAAGGCCGCCGCGC
TGATCAAGGCCATTCGCCCGTACGTGCAGAACTTCCACTCCAGCCAGTACGTGACCTCGCTGGCCAACGGCAATACCTGC
CTGGCGGTGGGTTGGTCCGGCGACATCATCCAGGCGCGCGACCGTGCCGCGGAAGCCGGCAACAACATCAAGGTGGCCTA
CGCCATTCCAAAGGAGGGCGCGCCGCAATGGTTCGACATGCTGGCCATTCCCAAGGATGCCAAGCACCCGGACAACGCCT
ACGCCTTCATCAATTATCTGTTGAAGCCGGACGTGGCCGCGGCCAATACCAACTTCATCCATTACGCCAACCCGGTGCGC
ACCGCCACCCCGTTGGTGGATGCGGCGATCCGCAACGACCCCACCATCTACCCGCCGCCGGAAGTGACCGCCAAGATGTT
CACCTATGCCATCAATCCGCCTGACGTGGACCGGCTGTACACGCGGCTGTGGACCGAGATCAAGACCGGGCATTGA

Upstream 100 bases:

>100_bases
TGCCGGCCTCGCTGCGCTGGGCAAATCCGGGCGTGGTGGGTAGGCTGGGAAGAGCCCGGATTCGAGACCGGCCGCCCCAT
CGCGCTGCCTGGAGACCCAC

Downstream 100 bases:

>100_bases
GCTGCTGCGTTGCCGGTGGATGCTGCGCCCGAGCGCAGTGCTGCCTCGTGCAACGGCCCATGCCACGGACCCGGCGCGCG
GCCTAGGGGTGCACCGTCGG

Product: periplasmic putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTLRLLALTLSITLLSGCGGAGAPGSADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVTYDVFDSDEMVETKLLAGGSD
YDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMRRIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVAN
SWDLVFKPENLSKLKDCGVTILDTPSDMIPIALNYLGLDPHSTTPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTC
LAVGWSGDIIQARDRAAEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYLLKPDVAAANTNFIHYANPVR
TATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLYTRLWTEIKTGH

Sequences:

>Translated_371_residues
MTLRLLALTLSITLLSGCGGAGAPGSADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVTYDVFDSDEMVETKLLAGGSD
YDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMRRIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVAN
SWDLVFKPENLSKLKDCGVTILDTPSDMIPIALNYLGLDPHSTTPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTC
LAVGWSGDIIQARDRAAEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYLLKPDVAAANTNFIHYANPVR
TATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLYTRLWTEIKTGH
>Mature_370_residues
TLRLLALTLSITLLSGCGGAGAPGSADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVTYDVFDSDEMVETKLLAGGSDY
DVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMRRIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVANS
WDLVFKPENLSKLKDCGVTILDTPSDMIPIALNYLGLDPHSTTPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTCL
AVGWSGDIIQARDRAAEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYLLKPDVAAANTNFIHYANPVRT
ATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLYTRLWTEIKTGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=358, Percent_Identity=52.2346368715084, Blast_Score=403, Evalue=1e-114,
Organism=Escherichia coli, GI1787367, Length=340, Percent_Identity=33.5294117647059, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787711, Length=321, Percent_Identity=27.1028037383178, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40350; Mature: 40219

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLLALTLSITLLSGCGGAGAPGSADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVT
CEEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCHHCCCCCEEE
YDVFDSDEMVETKLLAGGSDYDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMR
EEECCCCHHHHHHHCCCCCCCEEEECCHHHCCHHHCCCEEEECCHHHCCCCCCCCHHHHH
RIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVANSWDLVFKPENLSKLKDCGVT
HHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEE
ILDTPSDMIPIALNYLGLDPHSTTPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTC
EECCCCCCEEHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEE
LAVGWSGDIIQARDRAAEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYL
EEECCCCCEEHHHHHHHHCCCCEEEEEECCCCCCCHHHHHEECCCCCCCCCCHHHHHHHH
LKPDVAAANTNFIHYANPVRTATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLY
HCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHH
TRLWTEIKTGH
HHHHHHHCCCC
>Mature Secondary Structure 
TLRLLALTLSITLLSGCGGAGAPGSADAQAKVLNVYNYSDYIAEDTIPAFEKSTGIKVT
EEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCHHCCCCCEEE
YDVFDSDEMVETKLLAGGSDYDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMR
EEECCCCHHHHHHHCCCCCCCEEEECCHHHCCHHHCCCEEEECCHHHCCCCCCCCHHHHH
RIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVANSWDLVFKPENLSKLKDCGVT
HHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEE
ILDTPSDMIPIALNYLGLDPHSTTPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTC
EECCCCCCEEHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEE
LAVGWSGDIIQARDRAAEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYL
EEECCCCCEEHHHHHHHHCCCCEEEEEECCCCCCCHHHHHEECCCCCCCCCCHHHHHHHH
LKPDVAAANTNFIHYANPVRTATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLY
HCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHH
TRLWTEIKTGH
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]