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The map label for this gene is bioA [C]

Identifier: 21243210

GI number: 21243210

Start: 2883856

End: 2885223

Strand: Reverse

Name: bioA [C]

Synonym: XAC2477

Alternate gene names: 21243210

Gene position: 2885223-2883856 (Counterclockwise)

Preceding gene: 21243211

Following gene: 21243209

Centisome position: 55.75

GC content: 64.99

Gene sequence:

>1368_bases
GTGGATCCTTCGGTGCTCAAGGCGTTGCAGCAGCTCGACGCCGCCCATCATCTGCATCCGTTCAACGACAATGCGGCGTT
GGCGCAGAAGGGCACGCGCATCCTGACGCGCGGTGAGGGCGTGTACGTGTGGGATGCCGATGGCAACCAGTTGCTGGATG
CGTTCGGCGGATTGTGGTGCGTCAATGTCGGCTATGGGCGCAAGGAACTGGCGCAGGCAGCGGCAAGGCAGATGGAGCAG
CTGGCGTACTACAACAGCTTTTTCCAGTGCACCACCGAGCCCACCATTCAGTTGGCGGCCAAGCTGGCGGAGTTGGCGCC
GGGCGATCTGAATCATGCGTTCTTCGCCAACTCCGGGTCCGAGGCCAACGACACCATCCTGCGCCTGGTGCGGCATTTCT
GGGCCGTGCAGGGGCAGCCGCAAAAACGCATCTTCATCGGCCGCCATGATGGTTATCACGGCACCACCATGGCCGGCGCC
AGCCTGGGAGGCATGAGGGGAATGCATCAGCAGGGCGGGTTGCCGATTGCCGATATCCACCACGTCGCACCGCCGTATCA
CTTCGGCGATGGCGGGGAGATGGATCCGGAGGAATACGGGTTGCTGGCGGCACGTCGTCTGGAAGACAAGATTCTGGAAC
TCGGGCCGGATAACGTCGCCGCTTTTATCGGCGAGCCGATCATGGGCGCGACCGGCGTCTACATCCCACCGCGCAGTTAC
TGGCCGGAAATCGAGCGGATCTGCCGGCGCTACGACGTCTTGCTGGTCGCCGATGAAGTGATCTGCGGTTTCGGGCGTAC
TGGCGAATGGTTCGGTGCGGAGTATTTCGGCTTCCAGCCCGACGTGATGACGCTGGCCAAAGGCATCACTTCCGGCTACA
TCCCGTTGGCGGCAGCCATGTTCAACGACCGCGTGGCCGGTGTGCTCAAGACCCAAGGCGGCGAGCTGGCGCACGGTGGC
ACCTACGCCGGGCATCCGGTGTGCGCGGCGGTGGCGCTGGAAAACCTGCGCCTGCTGCAGGACGAGGGCATTGTCGAGAC
CGCGCGCACACAGGTTGCGCCATACCTGGCGCAGCGCTGGGCCGCGCTGGGCGAGCACCGCTTGGTCGGCGAGGCACGCA
TCGCCGGGCTGGTCGGGGCGCTGGAACTGGTGCCGGACAAACAGCGTCGCGGTGCGTATTTCCCAAGACGCGGGCGGGTA
GGGGCGATGTGTCGCGATTTCGCGCTGCGCCGCGGTCTGATCCTGCGCGCGACCTACGATGCCATGCTGCTGTCGCCGCC
GTTGATCATCACCCCCGCGCAGGTCGATGAATTGTTCGACAAGGCCTGGGCCGCGCTGGACGACACTGCGCAGGCGCTGG
GCCGATAG

Upstream 100 bases:

>100_bases
GCCCGGCGTGCAAGGGATTGGCCGGGCGATCTGCAAAGCCATCCACGGTCCGGCAACAGGGCCTGGGTATGCTGGTAACT
CCTCATGCGCGGAGCAGGTC

Downstream 100 bases:

>100_bases
CTCCCGCACAACCGGTCCCACTGCACGGCAGCGGGCCGCGACGAGGACGAGGACGAGTACGACCAAGCATGCGCACACAT
CGGTGCCATCGCGGCGGCTG

Product: aminotransferase

Products: N-Acetyl-L-glutamate 5-semialdehyde; L-Glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MDPSVLKALQQLDAAHHLHPFNDNAALAQKGTRILTRGEGVYVWDADGNQLLDAFGGLWCVNVGYGRKELAQAAARQMEQ
LAYYNSFFQCTTEPTIQLAAKLAELAPGDLNHAFFANSGSEANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGA
SLGGMRGMHQQGGLPIADIHHVAPPYHFGDGGEMDPEEYGLLAARRLEDKILELGPDNVAAFIGEPIMGATGVYIPPRSY
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAMFNDRVAGVLKTQGGELAHGG
TYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRWAALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPRRGRV
GAMCRDFALRRGLILRATYDAMLLSPPLIITPAQVDELFDKAWAALDDTAQALGR

Sequences:

>Translated_455_residues
MDPSVLKALQQLDAAHHLHPFNDNAALAQKGTRILTRGEGVYVWDADGNQLLDAFGGLWCVNVGYGRKELAQAAARQMEQ
LAYYNSFFQCTTEPTIQLAAKLAELAPGDLNHAFFANSGSEANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGA
SLGGMRGMHQQGGLPIADIHHVAPPYHFGDGGEMDPEEYGLLAARRLEDKILELGPDNVAAFIGEPIMGATGVYIPPRSY
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAMFNDRVAGVLKTQGGELAHGG
TYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRWAALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPRRGRV
GAMCRDFALRRGLILRATYDAMLLSPPLIITPAQVDELFDKAWAALDDTAQALGR
>Mature_455_residues
MDPSVLKALQQLDAAHHLHPFNDNAALAQKGTRILTRGEGVYVWDADGNQLLDAFGGLWCVNVGYGRKELAQAAARQMEQ
LAYYNSFFQCTTEPTIQLAAKLAELAPGDLNHAFFANSGSEANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGA
SLGGMRGMHQQGGLPIADIHHVAPPYHFGDGGEMDPEEYGLLAARRLEDKILELGPDNVAAFIGEPIMGATGVYIPPRSY
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAMFNDRVAGVLKTQGGELAHGG
TYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRWAALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPRRGRV
GAMCRDFALRRGLILRATYDAMLLSPPLIITPAQVDELFDKAWAALDDTAQALGR

Specific function: Involved In Both The Arginine And Lysine Biosynthetic Pathways. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=441, Percent_Identity=28.1179138321995, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4557809, Length=433, Percent_Identity=27.2517321016166, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI37574042, Length=430, Percent_Identity=27.2093023255814, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI226442705, Length=431, Percent_Identity=27.6102088167053, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI24119277, Length=439, Percent_Identity=27.5626423690205, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI226442709, Length=402, Percent_Identity=27.1144278606965, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI284507298, Length=329, Percent_Identity=28.8753799392097, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1789759, Length=404, Percent_Identity=28.4653465346535, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1786991, Length=444, Percent_Identity=27.9279279279279, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI145693181, Length=405, Percent_Identity=28.3950617283951, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789016, Length=449, Percent_Identity=26.7260579064588, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788044, Length=416, Percent_Identity=28.3653846153846, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787560, Length=314, Percent_Identity=30.2547770700637, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786349, Length=319, Percent_Identity=26.0188087774295, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32564660, Length=436, Percent_Identity=28.2110091743119, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25144271, Length=426, Percent_Identity=27.4647887323944, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71992977, Length=443, Percent_Identity=26.410835214447, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25144274, Length=248, Percent_Identity=29.8387096774194, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323470, Length=450, Percent_Identity=26.4444444444444, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324386, Length=446, Percent_Identity=25.1121076233184, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324432, Length=321, Percent_Identity=25.8566978193146, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356575, Length=417, Percent_Identity=30.4556354916067, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21357415, Length=431, Percent_Identity=28.0742459396752, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI28574759, Length=460, Percent_Identity=27.6086956521739, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI161085790, Length=448, Percent_Identity=27.9017857142857, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.11 [C]

Molecular weight: Translated: 49634; Mature: 49634

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPSVLKALQQLDAAHHLHPFNDNAALAQKGTRILTRGEGVYVWDADGNQLLDAFGGLWC
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCEEEEECCCCHHHHHHHCCEEE
VNVGYGRKELAQAAARQMEQLAYYNSFFQCTTEPTIQLAAKLAELAPGDLNHAFFANSGS
EECCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCCCCCEEEECCCC
EANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGASLGGMRGMHQQGGLPIADIH
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHH
HVAPPYHFGDGGEMDPEEYGLLAARRLEDKILELGPDNVAAFIGEPIMGATGVYIPPRSY
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEECCCCC
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAM
CHHHHHHHHHHCEEEEECHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHCCCCHHHHHHH
FNDRVAGVLKTQGGELAHGGTYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRW
HHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPRRGRVGAMCRDFALRRGLILRATYD
HHHCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHH
AMLLSPPLIITPAQVDELFDKAWAALDDTAQALGR
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDPSVLKALQQLDAAHHLHPFNDNAALAQKGTRILTRGEGVYVWDADGNQLLDAFGGLWC
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCEEEEECCCCHHHHHHHCCEEE
VNVGYGRKELAQAAARQMEQLAYYNSFFQCTTEPTIQLAAKLAELAPGDLNHAFFANSGS
EECCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCCCCCEEEECCCC
EANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGASLGGMRGMHQQGGLPIADIH
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHH
HVAPPYHFGDGGEMDPEEYGLLAARRLEDKILELGPDNVAAFIGEPIMGATGVYIPPRSY
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEECCCCC
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAM
CHHHHHHHHHHCEEEEECHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHCCCCHHHHHHH
FNDRVAGVLKTQGGELAHGGTYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRW
HHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPRRGRVGAMCRDFALRRGLILRATYD
HHHCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHH
AMLLSPPLIITPAQVDELFDKAWAALDDTAQALGR
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5-phosphate [C]

Metal ions: Ni2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.1 {N2-acetyl-L-ornithine}} 1.54 {N2-acetyl-L-ornithine}} 1.3 {N2-acetyl-L-ornithine}} 1.1 {N2-acetyl-L-ornithine}} 0.48 {N2-acetyl-L-ornithine}} 0.34 {N2-acetyl-L-ornithine}} 2.5 {2-oxoglutarate}} [C]

Substrates: N2-Acetyl-L-ornithine; 2-Oxoglutarate [C]

Specific reaction: N2-Acetyl-L-ornithine + 2-Oxoglutarate <==> N-Acetyl-L-glutamate 5-semialdehyde + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: Cu2+; Ornithine; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]