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The map label for this gene is kefB [H]

Identifier: 21243026

GI number: 21243026

Start: 2680128

End: 2681978

Strand: Reverse

Name: kefB [H]

Synonym: XAC2291

Alternate gene names: 21243026

Gene position: 2681978-2680128 (Counterclockwise)

Preceding gene: 21243027

Following gene: 21243024

Centisome position: 51.82

GC content: 64.24

Gene sequence:

>1851_bases
ATGAGCCAACCTGCCGAATCCAGTGAACTGATCAACGTGGTGGTGCTGCTCGGCGCTGCCGTGGTTGCGATCCCAGTGTT
CCGTCGTCTCGGCCTGGGCTCGGTGTTGGGTTATCTGGCAGCGGGACTTGCCATTGGTCCGTTCGGGTTGGGCCTGTTCA
ACGCGCCGCAGGCCATCCTGCATGTGGCCGAGTTGGGCGTGGTGATGTTTCTGTTCGTGATCGGGCTGGAAATGCGCCCC
TCGCACCTGTGGAGTTTACGCAAGGAAATCTTCGGACTTGGCGCGCTGCAGATCGCGGTTTGCGCGCTGGCGTTGACCGG
CGTCGGCATGTTGCTCGGCTTTGCGCTCCCGGTGGCCTTCGTGGCGGCGTCCGGTTTTGTGCTGACCTCCACCGCGGTGG
TGATGCAGTTGTTGGCCGAGCGCGGTGACGTCGCCTTGCCGCCGGGGCAGAAGATCGTGGCGATCCTGCTGTTCGAAGAT
CTGTTGATCGTGCCGTTGCTGGCGATCGTGGCCTGGATGGGGGCCAGCGGCGACCCGCAGGCCGCATCGCAGCGTTGGCA
GGGCATCGGCATCGGTCTGGCCTGCATCGTTGGCTTGCTGGTAGCCGGGCGTTGGCTGCTCAATCCGTTGTTTCGCATCC
TGGCTGCCTCCAAGGCGCGCGAGGTGATGACCGCTGCCGCGTTGCTGGTGGTGCTGGGCGCGGCACTGTTGATGCAGGTG
GGCGGGCTGTCGATGGCGATGGGCGCCTTTCTGGCCGGCGTGCTGTTGTCGGAATCCACATTCCGGCATCAGATCGAAGC
GGATATCGAACCGTTTCGCGGCATCCTGCTGGGGCTGTTTTTCCTGGGTGTCGGCATGAGCCTGGATCTGCGCGTGGTTG
CGGCCGATTGGCGCTTGATCGCGTCGGGCGTGCTGGCCTTGATGGTGGGCAAGGGCGCGTGTATCTATGGCGTGGCGCGG
CTGATGCGTAGCGACCATCGCCAGGCGCTGGACCGTGGCGTGCTGATGGCGCAAGGCGGCGAGTTCGCATTCGTGCTGTT
TGCCGCCGCGTCGGCATCGGGCGTCATCAATGCCCAAGTCAATGCCAGCCTGACCGCCATTGTGGTGTTGTCGATGGCAC
TGACGCCGTTGTTCGTGCTGCTGCAACGGCGGCTGACGCCTGCGGCGGCGGTCTCGCTGGATGGCGTGGAAGCAGCCAGT
GGCCAGACCGGCAGCGTGTTGATCATCGGCTTCGGGCGCTTCGGGCAGGTGGCCAGTCAGTCGCTGCTGGCGCGCGATGT
GGATGTGACCATCATCGATAACGACATCGAGATGATCCAAAGCGCGGAAGAATTCGGTTTCAAGATCTACTACGGCGACG
GCACCCGGCTCGATGTGTTGCACGCCTCCGGCGCACACAGCGCACGTGCGATTGCGGTGTGCGTCGACAATCAAGAGGCA
GCTAATCGCATCGTCGAGCTGGCAACGCGAGAGTTTCCGCATGCCAAGCTGCTGGTGCGTTCGTACGACCGCCAGCATTC
GCTGCAGTTGGTCGCGGCGGGAGTGGATTACCAGATCCGCGAGACCTTCGAGTCGGCGGTGATCTTCGGCAAAGTGGCGC
TGGTGGAGCTGGGTGTGGACGAGGACGAAGCCGGCATGATTTCGCGTGAGATCCGCCGCCGCGATGCAGAGCGTTTCGAA
CTGGAGATCGCTGCCGGCGATGCACGCGCCGGTGCCAGGCTGATGTATGGCAACACCGTGCCGACCGCACCCCGGCCCAC
GCCGTTCACGCGGCCGCGTCGCGGAAGTCGCACGCTGAATCCCGATGCGGTACCGCAGGCTGGGCAAGTAGAGACGCAGA
CGCGCGACTAG

Upstream 100 bases:

>100_bases
TTGTCGCGTCTGCAGAGCTCGGCTTGGCAACGCCAAGCGCGGGCAAAGATGCGCACCCTGATGCAGGCCGAGGCCGCGCC
GGTGCCGCTAGAATCCGGGC

Downstream 100 bases:

>100_bases
AGCGTGCGCTCCGGCTTCTCAGCGCGCCCATCTCCAGTCACAGATTGCTCCGGACATCTTGGCAGGCGCGCTCGCCGATG
CCTGCCTAGCCATGCTTGCG

Product: transport protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MSQPAESSELINVVVLLGAAVVAIPVFRRLGLGSVLGYLAAGLAIGPFGLGLFNAPQAILHVAELGVVMFLFVIGLEMRP
SHLWSLRKEIFGLGALQIAVCALALTGVGMLLGFALPVAFVAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFED
LLIVPLLAIVAWMGASGDPQAASQRWQGIGIGLACIVGLLVAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQV
GGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLIASGVLALMVGKGACIYGVAR
LMRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVINAQVNASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAAS
GQTGSVLIIGFGRFGQVASQSLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEA
ANRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVIFGKVALVELGVDEDEAGMISREIRRRDAERFE
LEIAAGDARAGARLMYGNTVPTAPRPTPFTRPRRGSRTLNPDAVPQAGQVETQTRD

Sequences:

>Translated_616_residues
MSQPAESSELINVVVLLGAAVVAIPVFRRLGLGSVLGYLAAGLAIGPFGLGLFNAPQAILHVAELGVVMFLFVIGLEMRP
SHLWSLRKEIFGLGALQIAVCALALTGVGMLLGFALPVAFVAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFED
LLIVPLLAIVAWMGASGDPQAASQRWQGIGIGLACIVGLLVAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQV
GGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLIASGVLALMVGKGACIYGVAR
LMRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVINAQVNASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAAS
GQTGSVLIIGFGRFGQVASQSLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEA
ANRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVIFGKVALVELGVDEDEAGMISREIRRRDAERFE
LEIAAGDARAGARLMYGNTVPTAPRPTPFTRPRRGSRTLNPDAVPQAGQVETQTRD
>Mature_615_residues
SQPAESSELINVVVLLGAAVVAIPVFRRLGLGSVLGYLAAGLAIGPFGLGLFNAPQAILHVAELGVVMFLFVIGLEMRPS
HLWSLRKEIFGLGALQIAVCALALTGVGMLLGFALPVAFVAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFEDL
LIVPLLAIVAWMGASGDPQAASQRWQGIGIGLACIVGLLVAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQVG
GLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLIASGVLALMVGKGACIYGVARL
MRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVINAQVNASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAASG
QTGSVLIIGFGRFGQVASQSLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEAA
NRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVIFGKVALVELGVDEDEAGMISREIRRRDAERFEL
EIAAGDARAGARLMYGNTVPTAPRPTPFTRPRRGSRTLNPDAVPQAGQVETQTRD

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=358, Percent_Identity=25.4189944134078, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789749, Length=557, Percent_Identity=42.5493716337522, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1786232, Length=572, Percent_Identity=39.5104895104895, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1786685, Length=495, Percent_Identity=27.8787878787879, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 65147; Mature: 65016

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPAESSELINVVVLLGAAVVAIPVFRRLGLGSVLGYLAAGLAIGPFGLGLFNAPQAIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHCCHHCCHHHHH
HVAELGVVMFLFVIGLEMRPSHLWSLRKEIFGLGALQIAVCALALTGVGMLLGFALPVAF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFEDLLIVPLLAIVAWMGASGDPQ
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AASQRWQGIGIGLACIVGLLVAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHH
ASGVLALMVGKGACIYGVARLMRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVINAQV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEHHCCCCEEEEEC
NASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAASGQTGSVLIIGFGRFGQVASQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCEEEEECCCHHHHHHH
SLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEA
HHHHCCCEEEEECCHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCHHH
ANRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVIFGKVALVELGVD
HHHHHHHHHHCCCHHHHHHHHHCCHHCEEEEEECCCHHHHHHHHHHHHHHHEEEEEECCC
EDEAGMISREIRRRDAERFELEIAAGDARAGARLMYGNTVPTAPRPTPFTRPRRGSRTLN
CCHHHHHHHHHHHCCCHHEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
PDAVPQAGQVETQTRD
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQPAESSELINVVVLLGAAVVAIPVFRRLGLGSVLGYLAAGLAIGPFGLGLFNAPQAIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHCCHHCCHHHHH
HVAELGVVMFLFVIGLEMRPSHLWSLRKEIFGLGALQIAVCALALTGVGMLLGFALPVAF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAASGFVLTSTAVVMQLLAERGDVALPPGQKIVAILLFEDLLIVPLLAIVAWMGASGDPQ
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AASQRWQGIGIGLACIVGLLVAGRWLLNPLFRILAASKAREVMTAAALLVVLGAALLMQV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLRVVAADWRLI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHH
ASGVLALMVGKGACIYGVARLMRSDHRQALDRGVLMAQGGEFAFVLFAAASASGVINAQV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEHHCCCCEEEEEC
NASLTAIVVLSMALTPLFVLLQRRLTPAAAVSLDGVEAASGQTGSVLIIGFGRFGQVASQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCEEEEECCCHHHHHHH
SLLARDVDVTIIDNDIEMIQSAEEFGFKIYYGDGTRLDVLHASGAHSARAIAVCVDNQEA
HHHHCCCEEEEECCHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCHHH
ANRIVELATREFPHAKLLVRSYDRQHSLQLVAAGVDYQIRETFESAVIFGKVALVELGVD
HHHHHHHHHHCCCHHHHHHHHHCCHHCEEEEEECCCHHHHHHHHHHHHHHHEEEEEECCC
EDEAGMISREIRRRDAERFELEIAAGDARAGARLMYGNTVPTAPRPTPFTRPRRGSRTLN
CCHHHHHHHHHHHCCCHHEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
PDAVPQAGQVETQTRD
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]