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The map label for this gene is folD

Identifier: 21243024

GI number: 21243024

Start: 2678761

End: 2679672

Strand: Reverse

Name: folD

Synonym: XAC2289

Alternate gene names: 21243024

Gene position: 2679672-2678761 (Counterclockwise)

Preceding gene: 21243026

Following gene: 21243023

Centisome position: 51.78

GC content: 67.43

Gene sequence:

>912_bases
ATGACCGCTTCTGCGCCAGCTGCCTCCGCTTCGGCCCGCCTTCTCGATGGTCGCCGCATTGCCGAGGAATTGCTCGACGG
ATTGAAGCTGCGGGTGGACGCGCGGGTGGCGGCCGGCAAGGCGCGCCCCGGGCTGGCGGTGGTGCTGGTCGGTGGCGATC
CGGCATCGTCGGTGTATGTGCGCAACAAACGGCGCGCGGCCGAGAAGGTCGGCATCGAAGCCTTCGATTACGACCTGCCG
CAGGGCACCAGCGAAGCCCAGTTGGCGTCGCTGATCGATGAGCTCAATGCCGATCCCAAGATCCACGGCATTCTGATCCA
GCTGCCGCTGCCGGGCATTCCCGATGCCAACCGGCTGATTCAGCGCATCGACCCGCGCAAGGACGTGGACGGATTCCATC
CGCAGAATGTCGGGCATCTGGCCTTGCGCGAATTCGGGCTGCGCCCGTGCACGCCGCGCGGCATCGTGACCTTGCTCGGC
CACACCGACCAGCCGGTGCGCGGCCGCAATGCCACCATCGTGGGCGTGAGCAATCACGTTGGCCGGCCGATGGGGCTGGA
GCTGTTGATCGCCGGCTGCACGGTCACCAGTTGCCACAAATTCACCCCGCCCGATGTGCTGGAAGCCAGCGTGCGCAATG
CGGACATCCTGGTGGTGGCAGTCGGCCGCCCGGGGCTGATTCCGGGCGAATGGGTCAAGCCGGGCGCGGTGGTGATCGAT
GTCGGCATCAACCGGCTCGATGATGGCCGCCTGGTGGGCGACGTCGGCTTCGACGCCGCCGCGCAGCGCGCGGCCTGGAT
CACCCCGGTGCCGGGCGGGGTAGGGCCGATGACGGTGGCCACGCTGATGCAGAACACCATCGAGGCCGCTGATGCGGCCG
GGATTCAGGATTCGGGATTCGGGATTCGTTAA

Upstream 100 bases:

>100_bases
CGCTTCGATCGAAGTAGTGCTCGAATCGGTGCGTGAGGTGGTGTCGGTCATGGCGGAATCGGGGACAATAGACGGCCTTC
GACTCTACACCTGACGCCCC

Downstream 100 bases:

>100_bases
AGGCAACGGCGAAGTGCCTGCATCGGCTGATCCGGATCGCATCCGAGATCGGCCGGCAATCGGCAAACAGGCAAACGGTG
AGCGGTGCAACGCGACGTTG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTASAPAASASARLLDGRRIAEELLDGLKLRVDARVAAGKARPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLP
QGTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLG
HTDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIQDSGFGIR

Sequences:

>Translated_303_residues
MTASAPAASASARLLDGRRIAEELLDGLKLRVDARVAAGKARPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLP
QGTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLG
HTDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIQDSGFGIR
>Mature_302_residues
TASAPAASASARLLDGRRIAEELLDGLKLRVDARVAAGKARPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLPQ
GTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGH
TDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVIDV
GINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIQDSGFGIR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=43.8356164383562, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=43.7710437710438, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=42.320819112628, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI36796743, Length=208, Percent_Identity=25.4807692307692, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=58.9285714285714, Blast_Score=332, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17568735, Length=256, Percent_Identity=42.578125, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=40.2684563758389, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=296, Percent_Identity=40.2027027027027, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322933, Length=288, Percent_Identity=25.6944444444444, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=46.9594594594595, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=47.2602739726027, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI62472483, Length=299, Percent_Identity=41.8060200668896, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI45551871, Length=299, Percent_Identity=41.8060200668896, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=42.2680412371134, Blast_Score=207, Evalue=5e-54,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=42.2680412371134, Blast_Score=207, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_XANAC (Q8PK86)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642606.1
- HSSP:   P11586
- ProteinModelPortal:   Q8PK86
- SMR:   Q8PK86
- GeneID:   1156360
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2289
- NMPDR:   fig|190486.1.peg.2250
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK10792
- BioCyc:   XAXO190486:XAC2289-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31774; Mature: 31643

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASAPAASASARLLDGRRIAEELLDGLKLRVDARVAAGKARPGLAVVLVGGDPASSVYV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEE
RNKRRAAEKVGIEAFDYDLPQGTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLI
ECHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHH
QRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGHTDQPVRGRNATIVGVSNHV
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECHHCC
GRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
CCCCCEEEEEECCCEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIQDSGF
ECCCCCCCCEEEEECCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GIR
CCC
>Mature Secondary Structure 
TASAPAASASARLLDGRRIAEELLDGLKLRVDARVAAGKARPGLAVVLVGGDPASSVYV
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEE
RNKRRAAEKVGIEAFDYDLPQGTSEAQLASLIDELNADPKIHGILIQLPLPGIPDANRLI
ECHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHH
QRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLGHTDQPVRGRNATIVGVSNHV
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECHHCC
GRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
CCCCCEEEEEECCCEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIQDSGF
ECCCCCCCCEEEEECCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GIR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217