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The map label for this gene is lysS

Identifier: 21242620

GI number: 21242620

Start: 2176503

End: 2178020

Strand: Direct

Name: lysS

Synonym: XAC1876

Alternate gene names: 21242620

Gene position: 2176503-2178020 (Clockwise)

Preceding gene: 21242619

Following gene: 21242621

Centisome position: 42.05

GC content: 65.02

Gene sequence:

>1518_bases
ATGACCGAGCAGACCCCCGCGCCGCAGCCCCCCGCCGACGAGAACAGCCTCATCGCCGAGCGCCGCGCGAAACTGGGCGC
GTTGCGCGGGCAGGGCATCGCCTACCCGAACGACTTCGTGCGCGAACACTTCGCCGGCGATCTGCAGGCAGAATTCGCCG
ACGCCGACACCTGGACCCCGGAAGCGCTGGAAGCCAGCGGGCGCACGGTCAGGATGGCCGGCCGCCTGATGGCCAAGCGG
GTGATGGGCAAGGCCAGCTTTGCGCAGATCCAGGACGAATCCGGGCGTGTGCAGCTGTTCCTGCAGGGCAATGTGCTCGG
CGATGCCTATACCGCGTTCAAGGGCTGGGATGTGGGCGACATCGTGGCGGTGGAGGGAGGGCTCACCCGCACCAAGACCG
GCGAGCTGTCGGTCAAGGCCAGCGCGCTGCGCCTGCTGACCAAGTCGCTGCGCCCGTTGCCGGACAAGTGGCACGGCCTG
TCGGATGTTGAGCAGCGTTACCGCCAGCGTTATGTCGACCTGATCGTGACACCGGAAGCGCGCGAGGTCTTCATCAAGCG
ATCCAAGATCATCCGCGCCATGCGCGCCTGGCTGGACGCGCGCCGCTTCCTGGAAGTGGAAACGCCGATGATGCATTACA
TTCCCGGCGGCGCCACGGCCAAGCCATTCACTACCCACCACAATGCGCTGGACCTGGACTTGTACCTCCGCGTCGCCCCA
GAGCTGTATCTCAAGCGTCTGGTGGTCGGTGGCCTGGAGCGCGTCTACGAGATCAACCGCAATTTCCGCAACGAAGGCGT
GTCGACCCGGCACAACCCCGAGTTCACCATGCTCGAGCTCTACGAGGCTTATGCCACGTACCACCAGATCATGGACCTGA
CCGAGCAGGTGATCCGCGACACCGCGCAGTCGGTGTTGGGTACCACGCAGGTGAGCTGGGATGGCGCGGACATCGATTTG
GCGCCGGCTTTCCGGCGCTGGCGCATGGATGAGGCCGTTCGTCACCATAATCCGGAGATCAGCGCTGCCGACTGCACCGA
CCGCGAGGCGCTGCTGCGCCATTGCGAGCGGCTCAAGATCCGCGTGAAGCCGTCTTACGGCTGGGGCAAGCTGCTGCTGG
AGATTTTCGAGGCGACCGTGGAGCACACCCTGGTGCAGCCGACCTTCATCACCGACCACCCGGTCGAAGTGTCGCCGCTG
GCGCGTTCCAGCGACACCGAGCCGGGTTACACCGACCGCTTCGAGCTGTTCATCAACGGTAAGGAGCTGGCCAACGGCTT
TTCCGAGCTCAACGACCCCGAAGACCAGGCCGCACGTTTCCAGGCGCAGGTGCAGGCCAAGGACGGCGGCGACGACGAAG
CGATGCATTTCGATGCCGATTACATCCGTGCCTTGGAGTACGGCATGGCGCCGACCGGCGGCCTGGGCATCGGTATCGAT
CGTCTGGTGATGCTGCTGACCGGCAGCACCTCGATCCGCGATGTCCTGCTATTCCCGTACATGCGTCCGGAAGCCTGA

Upstream 100 bases:

>100_bases
CCTGCCTCCGTCACATCCTCGTTCTAATAAGCGTGCCGGCACCGCCGGTTCCTCATTCCCCACCGGGCACCCGCCCGACC
CACAGCAGACCGATCCCGCC

Downstream 100 bases:

>100_bases
TTTTTTTGTGCCGGCGGTGTGCCGACGGCACAGATACTGCATATGCTAGGGCGGTTCCTCCGGACGGCGTATCGTCGGAG
GTAGCCGGCGCGGGGGCCGG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTEQTPAPQPPADENSLIAERRAKLGALRGQGIAYPNDFVREHFAGDLQAEFADADTWTPEALEASGRTVRMAGRLMAKR
VMGKASFAQIQDESGRVQLFLQGNVLGDAYTAFKGWDVGDIVAVEGGLTRTKTGELSVKASALRLLTKSLRPLPDKWHGL
SDVEQRYRQRYVDLIVTPEAREVFIKRSKIIRAMRAWLDARRFLEVETPMMHYIPGGATAKPFTTHHNALDLDLYLRVAP
ELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYATYHQIMDLTEQVIRDTAQSVLGTTQVSWDGADIDL
APAFRRWRMDEAVRHHNPEISAADCTDREALLRHCERLKIRVKPSYGWGKLLLEIFEATVEHTLVQPTFITDHPVEVSPL
ARSSDTEPGYTDRFELFINGKELANGFSELNDPEDQAARFQAQVQAKDGGDDEAMHFDADYIRALEYGMAPTGGLGIGID
RLVMLLTGSTSIRDVLLFPYMRPEA

Sequences:

>Translated_505_residues
MTEQTPAPQPPADENSLIAERRAKLGALRGQGIAYPNDFVREHFAGDLQAEFADADTWTPEALEASGRTVRMAGRLMAKR
VMGKASFAQIQDESGRVQLFLQGNVLGDAYTAFKGWDVGDIVAVEGGLTRTKTGELSVKASALRLLTKSLRPLPDKWHGL
SDVEQRYRQRYVDLIVTPEAREVFIKRSKIIRAMRAWLDARRFLEVETPMMHYIPGGATAKPFTTHHNALDLDLYLRVAP
ELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYATYHQIMDLTEQVIRDTAQSVLGTTQVSWDGADIDL
APAFRRWRMDEAVRHHNPEISAADCTDREALLRHCERLKIRVKPSYGWGKLLLEIFEATVEHTLVQPTFITDHPVEVSPL
ARSSDTEPGYTDRFELFINGKELANGFSELNDPEDQAARFQAQVQAKDGGDDEAMHFDADYIRALEYGMAPTGGLGIGID
RLVMLLTGSTSIRDVLLFPYMRPEA
>Mature_504_residues
TEQTPAPQPPADENSLIAERRAKLGALRGQGIAYPNDFVREHFAGDLQAEFADADTWTPEALEASGRTVRMAGRLMAKRV
MGKASFAQIQDESGRVQLFLQGNVLGDAYTAFKGWDVGDIVAVEGGLTRTKTGELSVKASALRLLTKSLRPLPDKWHGLS
DVEQRYRQRYVDLIVTPEAREVFIKRSKIIRAMRAWLDARRFLEVETPMMHYIPGGATAKPFTTHHNALDLDLYLRVAPE
LYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYATYHQIMDLTEQVIRDTAQSVLGTTQVSWDGADIDLA
PAFRRWRMDEAVRHHNPEISAADCTDREALLRHCERLKIRVKPSYGWGKLLLEIFEATVEHTLVQPTFITDHPVEVSPLA
RSSDTEPGYTDRFELFINGKELANGFSELNDPEDQAARFQAQVQAKDGGDDEAMHFDADYIRALEYGMAPTGGLGIGIDR
LVMLLTGSTSIRDVLLFPYMRPEA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=452, Percent_Identity=44.6902654867257, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI194272210, Length=452, Percent_Identity=44.6902654867257, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1790571, Length=507, Percent_Identity=56.4102564102564, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1789256, Length=506, Percent_Identity=56.1264822134387, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI87082379, Length=327, Percent_Identity=30.5810397553517, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788173, Length=248, Percent_Identity=27.8225806451613, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=467, Percent_Identity=44.1113490364026, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535925, Length=467, Percent_Identity=44.1113490364026, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71994340, Length=467, Percent_Identity=44.1113490364026, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32566633, Length=172, Percent_Identity=26.7441860465116, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=458, Percent_Identity=45.1965065502183, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324256, Length=473, Percent_Identity=32.1353065539112, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24640849, Length=490, Percent_Identity=42.0408163265306, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640851, Length=490, Percent_Identity=42.0408163265306, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24584738, Length=335, Percent_Identity=27.1641791044776, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_XANAC (Q8PLC6)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642202.1
- ProteinModelPortal:   Q8PLC6
- SMR:   Q8PLC6
- GeneID:   1155947
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1876
- NMPDR:   fig|190486.1.peg.1846
- HOGENOM:   HBG631383
- OMA:   GHPQMMS
- ProtClustDB:   PRK00484
- BioCyc:   XAXO190486:XAC1876-MONOMER
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56882; Mature: 56751

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQTPAPQPPADENSLIAERRAKLGALRGQGIAYPNDFVREHFAGDLQAEFADADTWTP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCC
EALEASGRTVRMAGRLMAKRVMGKASFAQIQDESGRVQLFLQGNVLGDAYTAFKGWDVGD
HHHHCCCCEEHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCCCCCC
IVAVEGGLTRTKTGELSVKASALRLLTKSLRPLPDKWHGLSDVEQRYRQRYVDLIVTPEA
EEEECCCCCCCCCCCEEHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHEEEECCHH
REVFIKRSKIIRAMRAWLDARRFLEVETPMMHYIPGGATAKPFTTHHNALDLDLYLRVAP
HHHHHHHHHHHHHHHHHHHHHHHEEECCCHHEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYATYHQIMDLTEQVIRD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TAQSVLGTTQVSWDGADIDLAPAFRRWRMDEAVRHHNPEISAADCTDREALLRHCERLKI
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCEE
RVKPSYGWGKLLLEIFEATVEHTLVQPTFITDHPVEVSPLARSSDTEPGYTDRFELFING
EEECCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECH
KELANGFSELNDPEDQAARFQAQVQAKDGGDDEAMHFDADYIRALEYGMAPTGGLGIGID
HHHHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCHH
RLVMLLTGSTSIRDVLLFPYMRPEA
HHHHHHCCCCCHHHHHEECCCCCCC
>Mature Secondary Structure 
TEQTPAPQPPADENSLIAERRAKLGALRGQGIAYPNDFVREHFAGDLQAEFADADTWTP
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCC
EALEASGRTVRMAGRLMAKRVMGKASFAQIQDESGRVQLFLQGNVLGDAYTAFKGWDVGD
HHHHCCCCEEHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCCCCCC
IVAVEGGLTRTKTGELSVKASALRLLTKSLRPLPDKWHGLSDVEQRYRQRYVDLIVTPEA
EEEECCCCCCCCCCCEEHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHEEEECCHH
REVFIKRSKIIRAMRAWLDARRFLEVETPMMHYIPGGATAKPFTTHHNALDLDLYLRVAP
HHHHHHHHHHHHHHHHHHHHHHHEEECCCHHEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYATYHQIMDLTEQVIRD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TAQSVLGTTQVSWDGADIDLAPAFRRWRMDEAVRHHNPEISAADCTDREALLRHCERLKI
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCEE
RVKPSYGWGKLLLEIFEATVEHTLVQPTFITDHPVEVSPLARSSDTEPGYTDRFELFING
EEECCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECH
KELANGFSELNDPEDQAARFQAQVQAKDGGDDEAMHFDADYIRALEYGMAPTGGLGIGID
HHHHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCEEECHHHHHHHHHCCCCCCCCCCCHH
RLVMLLTGSTSIRDVLLFPYMRPEA
HHHHHHCCCCCHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217