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The map label for this gene is rpfG [H]

Identifier: 21242621

GI number: 21242621

Start: 2178162

End: 2179298

Strand: Direct

Name: rpfG [H]

Synonym: XAC1877

Alternate gene names: 21242621

Gene position: 2178162-2179298 (Clockwise)

Preceding gene: 21242620

Following gene: 21242622

Centisome position: 42.09

GC content: 66.05

Gene sequence:

>1137_bases
ATGCAGGATGTTTTAGGGAATCCGGCCGGTGTATCTTCGGCGGATACATGGGGAAGCTGGTCGGAAAAGGCGGATCTGGG
ATTGAACATCGTCATTGTCGATGACCAGATGTCCGCGCGGACGATGCTGCGCCACGTCATCGAGGACATCGCGCCGGAGT
TGAAGGTCTACGATTTCGGCGACCCGCTCGATGCGCTGGCGTGGTGCGAGGCCGGGCGCGTGGATCTGCTGTTGCTGGAC
TACCGCATGCCCGGCATGGACGGGCTGGAGTTCGCGCGCCGCCTGCGCCGGCTGCCGAGCCACCGCGATATCCCGATCAT
CCTGATCACCATCGTCGGCGACGAGCCGATCCGCCAGGCGGCGCTGGAAGCGGGCGTCATCGATTTCCTGGTCAAGCCGA
TCCGTCCGCGCGAACTGCGCGCGCGCTGTTCCAACCTGTTGCAGCTGCGTCAGCAGAGCGAAAGCGTCAAGCAACGCGCG
CTCTCGCTGGAGCAGCGGCTGCTGGCGAGCATGAACGAGGTCGAGGAGCGTGAACGCGAGACCCTGTCGCGGCTGGCGCG
CGCCATCGAGTACCGCGACTCCGGCACCAGCGCGTTCCTGGAGCGCATGTCGCACGTGGCCGGGTTGATCGCCGAGCAGC
TGGGCCTGTCGGAGGAGGAGGTGCGCATCATCGAGATGGCCGCGCCGCTGCACGACATGGGCAAGATCGCCATTCCCGAT
GCGGTGCTGCTCAAGCCGGGCAAGCTCACCGACGACGAGATGAGCGTGATGCGCCGGCATCCCCGGATCGGCTACGAACT
GCTCAGCGGCAGCCAGAACCGGTTCATCCAGGTCGGCGCGTTGATCGCCCTGCGCCATCACGAGCGCTACGACGGCAGCG
GCTACCCGGACGGACTGGTCGGCGAGGCGATTCCGCTGGAGGCGCGCATCGTGGCGGTGGCCGATGTGTTCGACGCGCTG
CTGTCGCCGCGCCCCTACAAGGATGCCTGGACGATGGATGCCGCGCTTGCCTATCTCTACGCGCAACGCGGGCGTCTGTT
CGATCCGCGTTGCGTGGATGCGCTGCTGCGCGGACGGGCGCAGTTGGATCAGGTCTGCAACCAGTTCTCTACCGCATCGG
TGCGTCCAGGAGCATAG

Upstream 100 bases:

>100_bases
TATGCTAGGGCGGTTCCTCCGGACGGCGTATCGTCGGAGGTAGCCGGCGCGGGGGCCGGTGTCATCGCTTTCCCGTTCTC
GAAACCGAGGAGTAACGGAT

Downstream 100 bases:

>100_bases
GCCATGAATCCGTTCGCCAGGATCCGGCAAACGCTGATGAATCGGCCCGATAGCGAACATGGCCAGGCCATCGTCCGTAT
CGTGCTGATCACCCTGATCC

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MQDVLGNPAGVSSADTWGSWSEKADLGLNIVIVDDQMSARTMLRHVIEDIAPELKVYDFGDPLDALAWCEAGRVDLLLLD
YRMPGMDGLEFARRLRRLPSHRDIPIILITIVGDEPIRQAALEAGVIDFLVKPIRPRELRARCSNLLQLRQQSESVKQRA
LSLEQRLLASMNEVEERERETLSRLARAIEYRDSGTSAFLERMSHVAGLIAEQLGLSEEEVRIIEMAAPLHDMGKIAIPD
AVLLKPGKLTDDEMSVMRRHPRIGYELLSGSQNRFIQVGALIALRHHERYDGSGYPDGLVGEAIPLEARIVAVADVFDAL
LSPRPYKDAWTMDAALAYLYAQRGRLFDPRCVDALLRGRAQLDQVCNQFSTASVRPGA

Sequences:

>Translated_378_residues
MQDVLGNPAGVSSADTWGSWSEKADLGLNIVIVDDQMSARTMLRHVIEDIAPELKVYDFGDPLDALAWCEAGRVDLLLLD
YRMPGMDGLEFARRLRRLPSHRDIPIILITIVGDEPIRQAALEAGVIDFLVKPIRPRELRARCSNLLQLRQQSESVKQRA
LSLEQRLLASMNEVEERERETLSRLARAIEYRDSGTSAFLERMSHVAGLIAEQLGLSEEEVRIIEMAAPLHDMGKIAIPD
AVLLKPGKLTDDEMSVMRRHPRIGYELLSGSQNRFIQVGALIALRHHERYDGSGYPDGLVGEAIPLEARIVAVADVFDAL
LSPRPYKDAWTMDAALAYLYAQRGRLFDPRCVDALLRGRAQLDQVCNQFSTASVRPGA
>Mature_378_residues
MQDVLGNPAGVSSADTWGSWSEKADLGLNIVIVDDQMSARTMLRHVIEDIAPELKVYDFGDPLDALAWCEAGRVDLLLLD
YRMPGMDGLEFARRLRRLPSHRDIPIILITIVGDEPIRQAALEAGVIDFLVKPIRPRELRARCSNLLQLRQQSESVKQRA
LSLEQRLLASMNEVEERERETLSRLARAIEYRDSGTSAFLERMSHVAGLIAEQLGLSEEEVRIIEMAAPLHDMGKIAIPD
AVLLKPGKLTDDEMSVMRRHPRIGYELLSGSQNRFIQVGALIALRHHERYDGSGYPDGLVGEAIPLEARIVAVADVFDAL
LSPRPYKDAWTMDAALAYLYAQRGRLFDPRCVDALLRGRAQLDQVCNQFSTASVRPGA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=112, Percent_Identity=33.9285714285714, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=32.7868852459016, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=126, Percent_Identity=35.7142857142857, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 42200; Mature: 42200

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDVLGNPAGVSSADTWGSWSEKADLGLNIVIVDDQMSARTMLRHVIEDIAPELKVYDFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEECC
DPLDALAWCEAGRVDLLLLDYRMPGMDGLEFARRLRRLPSHRDIPIILITIVGDEPIRQA
CHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
ALEAGVIDFLVKPIRPRELRARCSNLLQLRQQSESVKQRALSLEQRLLASMNEVEERERE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSRLARAIEYRDSGTSAFLERMSHVAGLIAEQLGLSEEEVRIIEMAAPLHDMGKIAIPD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCCCCCCC
AVLLKPGKLTDDEMSVMRRHPRIGYELLSGSQNRFIQVGALIALRHHERYDGSGYPDGLV
CEEECCCCCCHHHHHHHHHCCCCCHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCC
GEAIPLEARIVAVADVFDALLSPRPYKDAWTMDAALAYLYAQRGRLFDPRCVDALLRGRA
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHH
QLDQVCNQFSTASVRPGA
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQDVLGNPAGVSSADTWGSWSEKADLGLNIVIVDDQMSARTMLRHVIEDIAPELKVYDFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEECC
DPLDALAWCEAGRVDLLLLDYRMPGMDGLEFARRLRRLPSHRDIPIILITIVGDEPIRQA
CHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
ALEAGVIDFLVKPIRPRELRARCSNLLQLRQQSESVKQRALSLEQRLLASMNEVEERERE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSRLARAIEYRDSGTSAFLERMSHVAGLIAEQLGLSEEEVRIIEMAAPLHDMGKIAIPD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCCCCCCC
AVLLKPGKLTDDEMSVMRRHPRIGYELLSGSQNRFIQVGALIALRHHERYDGSGYPDGLV
CEEECCCCCCHHHHHHHHHCCCCCHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCC
GEAIPLEARIVAVADVFDALLSPRPYKDAWTMDAALAYLYAQRGRLFDPRCVDALLRGRA
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHH
QLDQVCNQFSTASVRPGA
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]