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The map label for this gene is prfB [H]

Identifier: 21242619

GI number: 21242619

Start: 2175309

End: 2176298

Strand: Direct

Name: prfB [H]

Synonym: XAC1875

Alternate gene names: 21242619

Gene position: 2175309-2176298 (Clockwise)

Preceding gene: 21242618

Following gene: 21242620

Centisome position: 42.03

GC content: 63.84

Gene sequence:

>990_bases
GTGTGGAACGACGCCGAACGCGCGCAGGCCCTGGGCCGCGAGCGTTCGATGCTGGAAAAGACCGTGATCGGCATTGCCGA
TGTGCTGTCGGGCCTGGCCGATGCGGGCGACCTGCTGGAGCTGGCCGAGAGCGAACAGGACGAAGACACTGCGGTGGCGG
TGATCGCCGATCTGGACAACTACCAGGCGCATGTCGAAAAGCTGGAATTCCAGCGCATGTTCTCCGGCCAGATGGACGGC
GCCAACGCGTTCGTCGACATCCAGGCTGGCGCCGGCGGCACCGAGGCGCAGGACTGGGCCGAAATCCTGCTGCGCATGTA
TCTGCGCTGGGCCGAGTCGCGCGGCTGGAAAACCGAGTTGATGGAAGTCTCCGGTGGCGAAGTGGCGGGCATCAAGTCGG
CCACCGTGCGCATCGAGGGCGAATACGCCTACGGCTGGCTGAAGACCGAGATCGGCGTGCATCGGCTGGTGCGCAAATCG
CCGTTCGACTCGGACAACCGCCGGCATACCAGCTTCACCTCGGTGTTCGTGTCGCCGGAAGTGGACGACAACATCGAGAT
CGACATCAATCCGGCCGACCTGCGTACCGACGTGTATCGCTCGTCCGGTGCCGGTGGTCAGCACGTCAACAAGACCGAAT
CGGCGGTGCGTATCACGCACATCCCGACCAATACCGTGGTCGCCTGCCAGACCGGCCGCAGCCAGCACCAGAACCGCGAC
AACGCGATGAAAATGCTGGCCGCCAAGTTGTACGAGCTGGAAGTGCAAAAGCGCAACGCCGAGAAGGATGCCTTGGAAGC
GACCAAGTCCGACATCGGCTGGGGCAGCCAGATCCGCAATTACGTGCTCGACCAGAGCCGCATCAAGGATCTGCGCACCG
GCATCGAGCGCAGCGATACGCAGAAGGTGCTGGACGGCGACCTGGACGAATTCGTCGAGGCCAGCCTGAAGGCCGGTCTG
GCAGCGGGCTCCAAACGCCTGGATGCCTGA

Upstream 100 bases:

>100_bases
GATCTCAACGATCGCGTGCTCTCGCTCAGGGGGTATCTTTGACTACGACGCCAAGAAAGAGCGTCTAGAGGAAGTCAGCC
GGGAGCTCGAGAGTCCGGAT

Downstream 100 bases:

>100_bases
CCGCCGCGCCAGGTGACCGTCGCGACGGCATCCTGGCGCATCGCCGCAGTGCCTGTGACGCAGCGGTGGTCATGCGCTGC
GGCTTTAGGGTAACCCGATA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MWNDAERAQALGRERSMLEKTVIGIADVLSGLADAGDLLELAESEQDEDTAVAVIADLDNYQAHVEKLEFQRMFSGQMDG
ANAFVDIQAGAGGTEAQDWAEILLRMYLRWAESRGWKTELMEVSGGEVAGIKSATVRIEGEYAYGWLKTEIGVHRLVRKS
PFDSDNRRHTSFTSVFVSPEVDDNIEIDINPADLRTDVYRSSGAGGQHVNKTESAVRITHIPTNTVVACQTGRSQHQNRD
NAMKMLAAKLYELEVQKRNAEKDALEATKSDIGWGSQIRNYVLDQSRIKDLRTGIERSDTQKVLDGDLDEFVEASLKAGL
AAGSKRLDA

Sequences:

>Translated_329_residues
MWNDAERAQALGRERSMLEKTVIGIADVLSGLADAGDLLELAESEQDEDTAVAVIADLDNYQAHVEKLEFQRMFSGQMDG
ANAFVDIQAGAGGTEAQDWAEILLRMYLRWAESRGWKTELMEVSGGEVAGIKSATVRIEGEYAYGWLKTEIGVHRLVRKS
PFDSDNRRHTSFTSVFVSPEVDDNIEIDINPADLRTDVYRSSGAGGQHVNKTESAVRITHIPTNTVVACQTGRSQHQNRD
NAMKMLAAKLYELEVQKRNAEKDALEATKSDIGWGSQIRNYVLDQSRIKDLRTGIERSDTQKVLDGDLDEFVEASLKAGL
AAGSKRLDA
>Mature_329_residues
MWNDAERAQALGRERSMLEKTVIGIADVLSGLADAGDLLELAESEQDEDTAVAVIADLDNYQAHVEKLEFQRMFSGQMDG
ANAFVDIQAGAGGTEAQDWAEILLRMYLRWAESRGWKTELMEVSGGEVAGIKSATVRIEGEYAYGWLKTEIGVHRLVRKS
PFDSDNRRHTSFTSVFVSPEVDDNIEIDINPADLRTDVYRSSGAGGQHVNKTESAVRITHIPTNTVVACQTGRSQHQNRD
NAMKMLAAKLYELEVQKRNAEKDALEATKSDIGWGSQIRNYVLDQSRIKDLRTGIERSDTQKVLDGDLDEFVEASLKAGL
AAGSKRLDA

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=290, Percent_Identity=36.2068965517241, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI34577120, Length=297, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI166795305, Length=194, Percent_Identity=36.0824742268041, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI2367172, Length=320, Percent_Identity=68.4375, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=44.0329218106996, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=259, Percent_Identity=32.4324324324324, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=242, Percent_Identity=37.1900826446281, Blast_Score=145, Evalue=6e-36,
Organism=Drosophila melanogaster, GI19921226, Length=207, Percent_Identity=40.5797101449275, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36380; Mature: 36380

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNDAERAQALGRERSMLEKTVIGIADVLSGLADAGDLLELAESEQDEDTAVAVIADLDN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEEECCHH
YQAHVEKLEFQRMFSGQMDGANAFVDIQAGAGGTEAQDWAEILLRMYLRWAESRGWKTEL
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEH
MEVSGGEVAGIKSATVRIEGEYAYGWLKTEIGVHRLVRKSPFDSDNRRHTSFTSVFVSPE
EECCCCCEECEEEEEEEEECCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
VDDNIEIDINPADLRTDVYRSSGAGGQHVNKTESAVRITHIPTNTVVACQTGRSQHQNRD
CCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHHCCHH
NAMKMLAAKLYELEVQKRNAEKDALEATKSDIGWGSQIRNYVLDQSRIKDLRTGIERSDT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCCCCHH
QKVLDGDLDEFVEASLKAGLAAGSKRLDA
HHHHCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MWNDAERAQALGRERSMLEKTVIGIADVLSGLADAGDLLELAESEQDEDTAVAVIADLDN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEEECCHH
YQAHVEKLEFQRMFSGQMDGANAFVDIQAGAGGTEAQDWAEILLRMYLRWAESRGWKTEL
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEH
MEVSGGEVAGIKSATVRIEGEYAYGWLKTEIGVHRLVRKSPFDSDNRRHTSFTSVFVSPE
EECCCCCEECEEEEEEEEECCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
VDDNIEIDINPADLRTDVYRSSGAGGQHVNKTESAVRITHIPTNTVVACQTGRSQHQNRD
CCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHHCCHH
NAMKMLAAKLYELEVQKRNAEKDALEATKSDIGWGSQIRNYVLDQSRIKDLRTGIERSDT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCCCCHH
QKVLDGDLDEFVEASLKAGLAAGSKRLDA
HHHHCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA