Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 21242576

GI number: 21242576

Start: 2127974

End: 2128576

Strand: Direct

Name: hisH

Synonym: XAC1832

Alternate gene names: 21242576

Gene position: 2127974-2128576 (Clockwise)

Preceding gene: 21242575

Following gene: 21242577

Centisome position: 41.12

GC content: 66.83

Gene sequence:

>603_bases
ATGACCGACGTTGCGCTGATCGATGCCGGTGGCGCCAACCTGGGCTCGGTGCGCTATGCATTGGAGCGCTTGGGCGTCGA
CGCACGCTTGGTGCGCGATGCGGCTGGCCTGCAGGGCGCGCAGCGGGTGATCCTGCCCGGCGTCGGGGCGGCGCCAGAGG
CGATGTCGCGTCTGCGCGCGCAGGGCTTGGTCGAGCCGTTGCGGGAACTGCAGGTGCCGTTGATCGGCATCTGTCTGGGC
ATGCAGTTGCTGTTCGAACATTCCGAAGAAGGCGATGTCGAGTGCCTGGGTCTGCTGCCGGGCATCGTGCGGCACATGAC
GCCTGCGCTTGGCATTCGCATTCCGCACATGGGCTGGAACCAGCTGGTGCCGATGCGCGACTCCGCGTTGCTGGCCGGTC
TGCCCGAGCGCGCCAGTGCGTATTTCGTACATGGTTACGCGGCGCCGGTGACGGCCGACACCGTGGCCGCCTGCGATCAC
GGCGGGTTGTTCACCGCCGTGGTGCAGAACGGGCTGCGCTGCGGCGCGCAATTCCACCCTGAGCGTTCGGCCGAGACCGG
TGCACGCATCCTGCGCAATTTTCTTGAGATGAGCTTCCCATGA

Upstream 100 bases:

>100_bases
ATCACCACAAGGTGGAAGCCTGCTTCAAGGCCTTGGCGCGGGCATTGCGTCAGGCGATTCGCCGTGAGGGCACCGCGTTG
CCGACCACCAAGGGCACGCT

Downstream 100 bases:

>100_bases
GTTTCACCGTTTACCCGGCGCTGGATATCCGCAACGCTCGCGTGGTGCGCCTGCTGCAAGGCGATTACGCGCGCGAAACG
CAGTACGGCGACGACGTGCT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MTDVALIDAGGANLGSVRYALERLGVDARLVRDAAGLQGAQRVILPGVGAAPEAMSRLRAQGLVEPLRELQVPLIGICLG
MQLLFEHSEEGDVECLGLLPGIVRHMTPALGIRIPHMGWNQLVPMRDSALLAGLPERASAYFVHGYAAPVTADTVAACDH
GGLFTAVVQNGLRCGAQFHPERSAETGARILRNFLEMSFP

Sequences:

>Translated_200_residues
MTDVALIDAGGANLGSVRYALERLGVDARLVRDAAGLQGAQRVILPGVGAAPEAMSRLRAQGLVEPLRELQVPLIGICLG
MQLLFEHSEEGDVECLGLLPGIVRHMTPALGIRIPHMGWNQLVPMRDSALLAGLPERASAYFVHGYAAPVTADTVAACDH
GGLFTAVVQNGLRCGAQFHPERSAETGARILRNFLEMSFP
>Mature_199_residues
TDVALIDAGGANLGSVRYALERLGVDARLVRDAAGLQGAQRVILPGVGAAPEAMSRLRAQGLVEPLRELQVPLIGICLGM
QLLFEHSEEGDVECLGLLPGIVRHMTPALGIRIPHMGWNQLVPMRDSALLAGLPERASAYFVHGYAAPVTADTVAACDHG
GLFTAVVQNGLRCGAQFHPERSAETGARILRNFLEMSFP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=44.3877551020408, Blast_Score=165, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=30.3738317757009, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_XANAC (Q8PLG8)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642158.1
- ProteinModelPortal:   Q8PLG8
- SMR:   Q8PLG8
- GeneID:   1155903
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1832
- NMPDR:   fig|190486.1.peg.1802
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13141
- BioCyc:   XAXO190486:XAC1832-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 21270; Mature: 21139

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 78-78 ACT_SITE 179-179 ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVALIDAGGANLGSVRYALERLGVDARLVRDAAGLQGAQRVILPGVGAAPEAMSRLRA
CCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHH
QGLVEPLRELQVPLIGICLGMQLLFEHSEEGDVECLGLLPGIVRHMTPALGIRIPHMGWN
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHCEECCCCCCC
QLVPMRDSALLAGLPERASAYFVHGYAAPVTADTVAACDHGGLFTAVVQNGLRCGAQFHP
CCCCCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
ERSAETGARILRNFLEMSFP
CCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDVALIDAGGANLGSVRYALERLGVDARLVRDAAGLQGAQRVILPGVGAAPEAMSRLRA
CCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHH
QGLVEPLRELQVPLIGICLGMQLLFEHSEEGDVECLGLLPGIVRHMTPALGIRIPHMGWN
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHCEECCCCCCC
QLVPMRDSALLAGLPERASAYFVHGYAAPVTADTVAACDHGGLFTAVVQNGLRCGAQFHP
CCCCCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
ERSAETGARILRNFLEMSFP
CCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217