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The map label for this gene is hisA

Identifier: 21242577

GI number: 21242577

Start: 2128573

End: 2129307

Strand: Direct

Name: hisA

Synonym: XAC1833

Alternate gene names: 21242577

Gene position: 2128573-2129307 (Clockwise)

Preceding gene: 21242576

Following gene: 21242578

Centisome position: 41.13

GC content: 67.48

Gene sequence:

>735_bases
ATGAGTTTCACCGTTTACCCGGCGCTGGATATCCGCAACGCTCGCGTGGTGCGCCTGCTGCAAGGCGATTACGCGCGCGA
AACGCAGTACGGCGACGACGTGCTGCCGCGTGCGCAGGCATTCGCCGATGCCGGTGCGCAGTGGATGCATCTGGTCGATC
TGGATGCGGCCAAGGCGGGCGGCTACACCCTGGCCGGCACACTCGGCGAGATTGCGCGAGCCACCGGCTTGCGCGTGCAG
ACCGGCGGCGGTGTGCGCTCGCGCGAGGATGTGGCGCGCATCCTCGACGCAGGCGCCGCACGCGTGGTGATCGGCTCATT
GGCAGTGCGCAATAGCGAGATGGTGGTTGGCTGGCTGCAGGAATTCGGCGCCGATCGCCTGACGATTGCGCTGGACACGC
GCCAGGACGCCGATGGCATCTGGCAGTTGCCGGTGCACGGCTGGACCGAAGCTGCAGACGCCACGCTGGATCAATTGGCC
GTGCGCTACGCGCGAGCCGGGCTGCAGCATTTGCTGTGTACCGACATTGCCCGCGACGGCATGCTGTCCGGCCCCAACAT
GGCGCTGTACGGGCATTTGCGCGCGCTGACGCCGCAGTTGCAGGTGCAGGTCTCCGGGGGCGCGCGCAATCTGGCCGATG
TCGCAGCTGCCAAGGCCGCGGGCTGCGCCGGCATCGTGCTGGGCAAGGCCTTGCTCGAAGGGCATCTGAATCTCGACGAG
GCACTGGCATGCTGA

Upstream 100 bases:

>100_bases
TGGTGCAGAACGGGCTGCGCTGCGGCGCGCAATTCCACCCTGAGCGTTCGGCCGAGACCGGTGCACGCATCCTGCGCAAT
TTTCTTGAGATGAGCTTCCC

Downstream 100 bases:

>100_bases
GCCGTCGCATCATTCCGTGCCTGGACGTGCGCGATGGGCGCGTGGTCAAGGGCGTCAAGTTCCGCGATCACATCGACATG
GGCGACATCGTCGAACTGGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSFTVYPALDIRNARVVRLLQGDYARETQYGDDVLPRAQAFADAGAQWMHLVDLDAAKAGGYTLAGTLGEIARATGLRVQ
TGGGVRSREDVARILDAGAARVVIGSLAVRNSEMVVGWLQEFGADRLTIALDTRQDADGIWQLPVHGWTEAADATLDQLA
VRYARAGLQHLLCTDIARDGMLSGPNMALYGHLRALTPQLQVQVSGGARNLADVAAAKAAGCAGIVLGKALLEGHLNLDE
ALAC

Sequences:

>Translated_244_residues
MSFTVYPALDIRNARVVRLLQGDYARETQYGDDVLPRAQAFADAGAQWMHLVDLDAAKAGGYTLAGTLGEIARATGLRVQ
TGGGVRSREDVARILDAGAARVVIGSLAVRNSEMVVGWLQEFGADRLTIALDTRQDADGIWQLPVHGWTEAADATLDQLA
VRYARAGLQHLLCTDIARDGMLSGPNMALYGHLRALTPQLQVQVSGGARNLADVAAAKAAGCAGIVLGKALLEGHLNLDE
ALAC
>Mature_243_residues
SFTVYPALDIRNARVVRLLQGDYARETQYGDDVLPRAQAFADAGAQWMHLVDLDAAKAGGYTLAGTLGEIARATGLRVQT
GGGVRSREDVARILDAGAARVVIGSLAVRNSEMVVGWLQEFGADRLTIALDTRQDADGIWQLPVHGWTEAADATLDQLAV
RYARAGLQHLLCTDIARDGMLSGPNMALYGHLRALTPQLQVQVSGGARNLADVAAAKAAGCAGIVLGKALLEGHLNLDEA
LAC

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=47.9166666666667, Blast_Score=224, Evalue=3e-60,
Organism=Escherichia coli, GI1788336, Length=134, Percent_Identity=27.6119402985075, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_XANAC (Q8PLG7)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642159.1
- ProteinModelPortal:   Q8PLG7
- GeneID:   1155904
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1833
- NMPDR:   fig|190486.1.peg.1803
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   XAXO190486:XAC1833-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25800; Mature: 25668

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTVYPALDIRNARVVRLLQGDYARETQYGDDVLPRAQAFADAGAQWMHLVDLDAAKAG
CCEEEEECEECCCCCEEEEHHCCCHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
GYTLAGTLGEIARATGLRVQTGGGVRSREDVARILDAGAARVVIGSLAVRNSEMVVGWLQ
CEEEEHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
EFGADRLTIALDTRQDADGIWQLPVHGWTEAADATLDQLAVRYARAGLQHLLCTDIARDG
HHCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MLSGPNMALYGHLRALTPQLQVQVSGGARNLADVAAAKAAGCAGIVLGKALLEGHLNLDE
CCCCCCEEEEHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHH
ALAC
HHCC
>Mature Secondary Structure 
SFTVYPALDIRNARVVRLLQGDYARETQYGDDVLPRAQAFADAGAQWMHLVDLDAAKAG
CEEEEECEECCCCCEEEEHHCCCHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
GYTLAGTLGEIARATGLRVQTGGGVRSREDVARILDAGAARVVIGSLAVRNSEMVVGWLQ
CEEEEHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
EFGADRLTIALDTRQDADGIWQLPVHGWTEAADATLDQLAVRYARAGLQHLLCTDIARDG
HHCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MLSGPNMALYGHLRALTPQLQVQVSGGARNLADVAAAKAAGCAGIVLGKALLEGHLNLDE
CCCCCCEEEEHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHH
ALAC
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217