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The map label for this gene is panB

Identifier: 21242530

GI number: 21242530

Start: 2054333

End: 2055148

Strand: Direct

Name: panB

Synonym: XAC1785

Alternate gene names: 21242530

Gene position: 2054333-2055148 (Clockwise)

Preceding gene: 21242529

Following gene: 21242531

Centisome position: 39.69

GC content: 68.01

Gene sequence:

>816_bases
ATGAGCAGCCACGCCGACAGCAAGCCCTGGACCGTGCCCGCCTTGGCGCAGGCCAAGCGCGAGGGTCGAAAACTGGTCAT
GTTGACCGCGTACGACGCCGGTTTCGCGCGCACCTTCGACGCCAATGGCGTGGACCTGATCCTGGTCGGCGATTCGCTCG
GCATGGTGGTGCAGGGGCACGATTCCACCTTGCCGGTGACCACCGCCGACATGGTCTACCACACGGCTTCGGTTGCCCGT
GCGCTCGATCGCGCCTTGCTGGTGGCGGACCTGTCGTTCCAGGCCGACGCCACGCCTGAGCGTGCATTGGACGCCGCCAC
CCAGCTGCTGCAGGCTGGCGCGGAGATGGTCAAGATCGAAGGCGCCGGGCACAAGCTGGAGGTCATCCGTTACCTGGTCG
AGCGCGAGATCCCGGTCTGCTCGCACCTGGGCCTGACCCCGCAATCGGTGCTGCGGTTCGGTGGCTACAAGGTGCAGGGG
CGCGGCGAGGCTGGCGAGCAGCTGCGTCGTGACGCACAGGCCGTGGTCGACGCCGGTGCCAGCCTGTTGGTGCTGGAATG
CGTGCCGACGCCGATCGCCACGCAGATTAGTGCCGAGCTCAGCGTGCCCACCATCGGCATCGGTGCCGGCCCCGGTTGCG
ACGGCCAGGTGCTGGTGATGCACGACATGCTCGGGCTGGACAGTGGCCACCGCCGGCCCAAGTTCGTCAAGGATTTCCTG
GCCGAAGGCGGCTCGGTTGCCGGCGCGGTGCGCGCCTATGCACAGGCGGTGCGCGACGGCAGTTTTCCCGATGCAGAGCA
CGCGTACGCCGCATGA

Upstream 100 bases:

>100_bases
GGCATGGAACAGTGCGGCGCGCCCTGCACGCCATCGATACCTGCGGGCTGGAGCCGATTGGGTAGATAATGGCCGGCTTA
TCACCCCACGTAATGACTTC

Downstream 100 bases:

>100_bases
TCCAGACCCTGACCGATCTTTCCGCACTGCGCGCCCTGGTCAATGGCTGGAAGCGCGAGGGCTTGCGTGTGGCGCTGGTG
CCTACCATGGGCAACCTGCA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSSHADSKPWTVPALAQAKREGRKLVMLTAYDAGFARTFDANGVDLILVGDSLGMVVQGHDSTLPVTTADMVYHTASVAR
ALDRALLVADLSFQADATPERALDAATQLLQAGAEMVKIEGAGHKLEVIRYLVEREIPVCSHLGLTPQSVLRFGGYKVQG
RGEAGEQLRRDAQAVVDAGASLLVLECVPTPIATQISAELSVPTIGIGAGPGCDGQVLVMHDMLGLDSGHRRPKFVKDFL
AEGGSVAGAVRAYAQAVRDGSFPDAEHAYAA

Sequences:

>Translated_271_residues
MSSHADSKPWTVPALAQAKREGRKLVMLTAYDAGFARTFDANGVDLILVGDSLGMVVQGHDSTLPVTTADMVYHTASVAR
ALDRALLVADLSFQADATPERALDAATQLLQAGAEMVKIEGAGHKLEVIRYLVEREIPVCSHLGLTPQSVLRFGGYKVQG
RGEAGEQLRRDAQAVVDAGASLLVLECVPTPIATQISAELSVPTIGIGAGPGCDGQVLVMHDMLGLDSGHRRPKFVKDFL
AEGGSVAGAVRAYAQAVRDGSFPDAEHAYAA
>Mature_270_residues
SSHADSKPWTVPALAQAKREGRKLVMLTAYDAGFARTFDANGVDLILVGDSLGMVVQGHDSTLPVTTADMVYHTASVARA
LDRALLVADLSFQADATPERALDAATQLLQAGAEMVKIEGAGHKLEVIRYLVEREIPVCSHLGLTPQSVLRFGGYKVQGR
GEAGEQLRRDAQAVVDAGASLLVLECVPTPIATQISAELSVPTIGIGAGPGCDGQVLVMHDMLGLDSGHRRPKFVKDFLA
EGGSVAGAVRAYAQAVRDGSFPDAEHAYAA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=263, Percent_Identity=55.893536121673, Blast_Score=290, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6319653, Length=264, Percent_Identity=36.7424242424242, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_XANAC (Q8PLL1)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642112.1
- ProteinModelPortal:   Q8PLL1
- SMR:   Q8PLL1
- GeneID:   1155856
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1785
- NMPDR:   fig|190486.1.peg.1756
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   XAXO190486:XAC1785-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28500; Mature: 28369

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: ACT_SITE 186-186 BINDING 90-90 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHADSKPWTVPALAQAKREGRKLVMLTAYDAGFARTFDANGVDLILVGDSLGMVVQGH
CCCCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCEEEECCCCEEEEEECCCCCEEEECC
DSTLPVTTADMVYHTASVARALDRALLVADLSFQADATPERALDAATQLLQAGAEMVKIE
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
GAGHKLEVIRYLVEREIPVCSHLGLTPQSVLRFGGYKVQGRGEAGEQLRRDAQAVVDAGA
CCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCC
SLLVLECVPTPIATQISAELSVPTIGIGAGPGCDGQVLVMHDMLGLDSGHRRPKFVKDFL
CEEEEEECCCCHHHHHEEECCCCEEECCCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHHH
AEGGSVAGAVRAYAQAVRDGSFPDAEHAYAA
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSHADSKPWTVPALAQAKREGRKLVMLTAYDAGFARTFDANGVDLILVGDSLGMVVQGH
CCCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCCEEEECCCCEEEEEECCCCCEEEECC
DSTLPVTTADMVYHTASVARALDRALLVADLSFQADATPERALDAATQLLQAGAEMVKIE
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
GAGHKLEVIRYLVEREIPVCSHLGLTPQSVLRFGGYKVQGRGEAGEQLRRDAQAVVDAGA
CCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCC
SLLVLECVPTPIATQISAELSVPTIGIGAGPGCDGQVLVMHDMLGLDSGHRRPKFVKDFL
CEEEEEECCCCHHHHHEEECCCCEEECCCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHHH
AEGGSVAGAVRAYAQAVRDGSFPDAEHAYAA
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217