Click here to switch to the map view.

The map label for this gene is dapE

Identifier: 21242185

GI number: 21242185

Start: 1650289

End: 1651419

Strand: Direct

Name: dapE

Synonym: XAC1432

Alternate gene names: 21242185

Gene position: 1650289-1651419 (Clockwise)

Preceding gene: 21242184

Following gene: 21242186

Centisome position: 31.89

GC content: 67.29

Gene sequence:

>1131_bases
ATGAGCGATGTGCTGGAGTTGACCTGTGACCTCATTGCACGTGCCTCGATAACGCCGGCGGATGCCGGTTGTCAGGCCGC
GATTGCGCAGCGCCTGCGCGCAGCAGGCTTCAGCTGCGAGCACTTGCGCCTGGGCGAGGTCGAGAATCTGTGGGCAACCC
ATGGCAGCGGTGCGCCGGTGCTGGTGTTGCTCGGGCATACCGATGTGGTGCCGCCGGGCCCGCGCGAGGCGTGGACCAGC
GCCCCGTTCGATCCGCAGATCCGCGACGGCGTGCTGTATGGGCGTGGTGCGGCGGACATGAAAGGCAGCGTCGCCGCATT
CGTCGTCGCCGCAGAGCAATTCGTCGCTGCGCATCCATCCCATACAGGCACGCTGGCGGTGTTGCTGACCTCGGACGAAG
AGGGCGATGCCATCGATGGCGTGCGCCGGGTTGCCGAGGTGTTTCGCGAACGCGGGCAAACGATCGACTGGTGCATCACC
GGCGAACCGTCGTCCACCGAGCGCCTGGGCGATCTGTTGCGCGTCGGCCGTCGCGGCAGCCTGTCCGGTACGTTGACCGT
CAAGGGTGTGCAGGGGCATGTGGCGTATCCGCACAAGGCGCGCAACCCGATCCACCTGGCAGCGCCTGCGCTGGCCGAAC
TGGTGGCGCGGCAGTGGGACGACGGCTTCGAAAGCTTTCCGCCCACCAGCCTGCAACTCTCCAACATCCATGCCGGCACC
GGCGCCAATAACGTGATTCCTGGCGAGCTGCAGGTGGCCTTCAATCTGCGCTATACCCCGCACTGGGACGCGCCGCGCCT
GGAGGCCGAAATCACCGCGCTGCTCGATCGGCACGCGCTGGACTACGCGCTGCGCTGGCATCGCAGCGGCGAGCCGTTCT
ACACCCCGGAAGGGCGGTTGCGCAGCGTTGCACGTGAGGTATTGGGCGAGTTCGCCGGCGCGCCGCCGGAAGAAAGCACC
GGTGGGGGCACCTCCGATGCGCGCTTCATCGCGCCATTGGGCGCGCAGTGCATCGAGGTGGGCCCGGTCAATGCCAGCAT
CCACCAGGTCGACGAGCATGTGCGCGTGGCCGACCTGCAAGCGCTACCGGCGCTGTATCGCAAGTTGATCGAGCGCCTGC
TGGTCGGGTGA

Upstream 100 bases:

>100_bases
ACCGCTGCAACCGCTGCAACAGCGACCAAAATACGCCGATCGATGCAGACGCAATCCCTCCATCAACGCCGCAGATGCGG
CTCTATTGCGGTGACGTTTC

Downstream 100 bases:

>100_bases
GCGGGCCACGCAGACGCCGTTGTTGCCACTGAAACAGTTGCGTCGGTGCAGCCGAACCGTTAGCGTCGGCGACATGGTCA
TTCGCCTCGCCAATCTCGAT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSDVLELTCDLIARASITPADAGCQAAIAQRLRAAGFSCEHLRLGEVENLWATHGSGAPVLVLLGHTDVVPPGPREAWTS
APFDPQIRDGVLYGRGAADMKGSVAAFVVAAEQFVAAHPSHTGTLAVLLTSDEEGDAIDGVRRVAEVFRERGQTIDWCIT
GEPSSTERLGDLLRVGRRGSLSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQLSNIHAGT
GANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRLRSVAREVLGEFAGAPPEEST
GGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQALPALYRKLIERLLVG

Sequences:

>Translated_376_residues
MSDVLELTCDLIARASITPADAGCQAAIAQRLRAAGFSCEHLRLGEVENLWATHGSGAPVLVLLGHTDVVPPGPREAWTS
APFDPQIRDGVLYGRGAADMKGSVAAFVVAAEQFVAAHPSHTGTLAVLLTSDEEGDAIDGVRRVAEVFRERGQTIDWCIT
GEPSSTERLGDLLRVGRRGSLSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQLSNIHAGT
GANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRLRSVAREVLGEFAGAPPEEST
GGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQALPALYRKLIERLLVG
>Mature_375_residues
SDVLELTCDLIARASITPADAGCQAAIAQRLRAAGFSCEHLRLGEVENLWATHGSGAPVLVLLGHTDVVPPGPREAWTSA
PFDPQIRDGVLYGRGAADMKGSVAAFVVAAEQFVAAHPSHTGTLAVLLTSDEEGDAIDGVRRVAEVFRERGQTIDWCITG
EPSSTERLGDLLRVGRRGSLSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQLSNIHAGTG
ANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRLRSVAREVLGEFAGAPPEESTG
GGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQALPALYRKLIERLLVG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=371, Percent_Identity=53.099730458221, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1790395, Length=205, Percent_Identity=34.1463414634146, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_XANAC (Q8PMJ5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641767.1
- ProteinModelPortal:   Q8PMJ5
- SMR:   Q8PMJ5
- MEROPS:   M20.010
- GeneID:   1155503
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1432
- NMPDR:   fig|190486.1.peg.1411
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   XAXO190486:XAC1432-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40298; Mature: 40167

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2; PS00605 ATPASE_C

Important sites: ACT_SITE 68-68 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVLELTCDLIARASITPADAGCQAAIAQRLRAAGFSCEHLRLGEVENLWATHGSGAPV
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCCE
LVLLGHTDVVPPGPREAWTSAPFDPQIRDGVLYGRGAADMKGSVAAFVVAAEQFVAAHPS
EEEECCCCCCCCCCHHHCCCCCCCCCHHCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCC
HTGTLAVLLTSDEEGDAIDGVRRVAEVFRERGQTIDWCITGEPSSTERLGDLLRVGRRGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCC
LSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQLSNIHAGT
CCEEEEEEECCCCEECCCCCCCCEEEHHHHHHHHHHHHCCCCHHCCCCCCEEEECEECCC
GANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRL
CCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RSVAREVLGEFAGAPPEESTGGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHH
ALPALYRKLIERLLVG
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVLELTCDLIARASITPADAGCQAAIAQRLRAAGFSCEHLRLGEVENLWATHGSGAPV
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCCE
LVLLGHTDVVPPGPREAWTSAPFDPQIRDGVLYGRGAADMKGSVAAFVVAAEQFVAAHPS
EEEECCCCCCCCCCHHHCCCCCCCCCHHCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCC
HTGTLAVLLTSDEEGDAIDGVRRVAEVFRERGQTIDWCITGEPSSTERLGDLLRVGRRGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCC
LSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQLSNIHAGT
CCEEEEEEECCCCEECCCCCCCCEEEHHHHHHHHHHHHCCCCHHCCCCCCEEEECEECCC
GANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRL
CCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RSVAREVLGEFAGAPPEESTGGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHH
ALPALYRKLIERLLVG
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217