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The map label for this gene is asnB [H]

Identifier: 21242186

GI number: 21242186

Start: 1651713

End: 1653407

Strand: Direct

Name: asnB [H]

Synonym: XAC1433

Alternate gene names: 21242186

Gene position: 1651713-1653407 (Clockwise)

Preceding gene: 21242185

Following gene: 21242191

Centisome position: 31.91

GC content: 63.54

Gene sequence:

>1695_bases
ATGTGTTCCATCTTCGGTATTTTCGGCCTGCAACCCGGTGACGACCTGCAGGCGCTGCGCCGGCAGGCGCTGGAATGTTC
ACAGCGGCAGCGTCACCGCGGGCCCGACTGGAGCGGCGTCTACGTCGATACCGGCGCCATCCTGGTGCACGAGCGCCTGG
CCATTGTCGATCCGGCCGGCGGCTCGCAACCATTGCTCTCCGAAGACGGGCAGCTCGCCCTGGCGGTCAATGGCGAGATC
TACAACCATCGCGAACTCAAGGCGGAGCTGCTGCAGCCGTACGCGTTCCAGACCGGCTCCGATTGCGAGGTGATCAATGC
GCTGTATCGCGAAGATACGCCTGCTTCGTATCTCAACCGCCTCAACGGCATCTTTGCATTCGCGCTGTGGGACAAGACGG
AAGGGCGCGTCATCATCGCGCGCGACCCGATCGGTGTGGTGCCGCTGTACTGGGGGCATGATCGCGAAGGCCGTTTGCGT
GTGGCGTCGGAACTGAAATCGCTGGTGGACGACTGTGCCGATGCGGCGCAGTTTCCGCCGGGCCATTGGTACGACAGCGC
CACCGGCGCACTGAACCGTTACTACGAACGCGCCTGGCGCCAATACGGCGCGGTGGAAGGTGTACAGGTGCAACTGCAGG
AATTGCGCGAAGCCTTCGAGCGCGCCGTGCACCGTCAGCTCATGACCGATGTGCCGTACGGTGTATTGCTGTCCGGTGGA
CTGGATTCTTCGCTGGTTGCGGCCGTCGCCGCGCGCTACGCCCGTCACCGCATCGAAGAAAACGACATCACCGAAGCCTG
GTGGCCGCGCCTGCATTCGTTCGCGATCGGCTTGAAGGGCTCGCCAGATCTGGCCGCGGCCGAAGTGGCCGCCGCAGCGC
TCGGCACGGTGCATCACGGTTTCGAATACACCTTCGACGAAGGCCTGGATGCATTGCCCGACGTCATTCGCCACATCGAA
ACCTATGACGTCACCACCATTCGCGCCTCCACACCGATGTTCTTGCTGGCGCGGCGGATCAAGGCGATGGGCGTCAAGAT
GGTGCTTTCCGGCGAAGGCAGCGATGAGATCTTCGGCGGCTACCTGTATTTCCATAAGGCACCGAATGCGCGCGAATTCC
ACGAAGAGCTGGTACGCAAGCTCGACGCGCTCAACAACTACGACTGCCTGCGCGCGAACAAGTCGATGATGGCCTGGGGC
GTAGAGCCACGTGTGCCGTTCCTGGACCGCGAATTCCTGGATGTGGCGATGCGCATGGATGCCAGGTTCAAGATGATCGA
CAAGACCAGCAGCGGGGCGACGCGCATGGAGAAGGGTGTACTGCGCGAGGCATTTGCCGGCTACCTGCCCGAATCCATCC
TGTGGCGTCAGAAAGAGCAGTTCAGCGACGGCGTGGGTTACGGCTGGATCGACGGGCTGAAGGCGCATGCGCAGTCGCAG
GTCAGCGACCGCGAACTCGCCGCCGCCGACCGACGCTACCCGGTCAACCCGCCGCAGACCAAGGAGGCGTATTTCTATCG
CAGCTTGTTCGAGCAGTTTTTCCCTGGCCAGGCGGCGGCGGAAACCGTGCCGGGCGGCAAGTCGATCGCCTGCTCGTCGC
CAACGGCGATTGCCTGGGATGCGAGTTTTGCCGCGATGGCCGACCCATCGGGCCGCGCGATTGCCGGGGTGCACGCGCAG
GCGCTGGCTTCGTAA

Upstream 100 bases:

>100_bases
AAAACCCGCATCGGCCGATGCGGGTTTTTTTTATGGGTGTCGATCCGTGCAGCACGCTCTTACCGGCCGCCAATCCACGC
AGTTCTTCAGGAGTGTTTCC

Downstream 100 bases:

>100_bases
AGGCTGCAGTCACTGCAGCGGCCGGCGTGCATCGCTGCACGGCCGGCCGCTGCGTTGACTGGCGCGTCTTATTGCTCGTG
CCGCACGCAGTACCCATAGC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDTGAILVHERLAIVDPAGGSQPLLSEDGQLALAVNGEI
YNHRELKAELLQPYAFQTGSDCEVINALYREDTPASYLNRLNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLR
VASELKSLVDDCADAAQFPPGHWYDSATGALNRYYERAWRQYGAVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
LDSSLVAAVAARYARHRIEENDITEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHGFEYTFDEGLDALPDVIRHIE
TYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWG
VEPRVPFLDREFLDVAMRMDARFKMIDKTSSGATRMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAQSQ
VSDRELAAADRRYPVNPPQTKEAYFYRSLFEQFFPGQAAAETVPGGKSIACSSPTAIAWDASFAAMADPSGRAIAGVHAQ
ALAS

Sequences:

>Translated_564_residues
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDTGAILVHERLAIVDPAGGSQPLLSEDGQLALAVNGEI
YNHRELKAELLQPYAFQTGSDCEVINALYREDTPASYLNRLNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLR
VASELKSLVDDCADAAQFPPGHWYDSATGALNRYYERAWRQYGAVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
LDSSLVAAVAARYARHRIEENDITEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHGFEYTFDEGLDALPDVIRHIE
TYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWG
VEPRVPFLDREFLDVAMRMDARFKMIDKTSSGATRMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAQSQ
VSDRELAAADRRYPVNPPQTKEAYFYRSLFEQFFPGQAAAETVPGGKSIACSSPTAIAWDASFAAMADPSGRAIAGVHAQ
ALAS
>Mature_564_residues
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDTGAILVHERLAIVDPAGGSQPLLSEDGQLALAVNGEI
YNHRELKAELLQPYAFQTGSDCEVINALYREDTPASYLNRLNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLR
VASELKSLVDDCADAAQFPPGHWYDSATGALNRYYERAWRQYGAVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
LDSSLVAAVAARYARHRIEENDITEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHGFEYTFDEGLDALPDVIRHIE
TYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWG
VEPRVPFLDREFLDVAMRMDARFKMIDKTSSGATRMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAQSQ
VSDRELAAADRRYPVNPPQTKEAYFYRSLFEQFFPGQAAAETVPGGKSIACSSPTAIAWDASFAAMADPSGRAIAGVHAQ
ALAS

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=553, Percent_Identity=36.5280289330922, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI168229252, Length=553, Percent_Identity=36.5280289330922, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI168229250, Length=553, Percent_Identity=36.5280289330922, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI296010848, Length=522, Percent_Identity=36.7816091954023, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI296010852, Length=456, Percent_Identity=36.6228070175439, Blast_Score=256, Evalue=5e-68,
Organism=Homo sapiens, GI296010850, Length=456, Percent_Identity=36.6228070175439, Blast_Score=256, Evalue=5e-68,
Organism=Escherichia coli, GI1786889, Length=561, Percent_Identity=59.5365418894831, Blast_Score=681, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=563, Percent_Identity=44.0497335701599, Blast_Score=447, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71993933, Length=567, Percent_Identity=43.7389770723104, Blast_Score=443, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17560178, Length=529, Percent_Identity=34.0264650283554, Blast_Score=287, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI25147560, Length=342, Percent_Identity=38.5964912280702, Blast_Score=214, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6321563, Length=597, Percent_Identity=49.748743718593, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325403, Length=594, Percent_Identity=48.989898989899, Blast_Score=523, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45553209, Length=577, Percent_Identity=45.4072790294627, Blast_Score=462, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62586; Mature: 62586

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDTGAILVHERLAIVDPAG
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCEEEECCCC
GSQPLLSEDGQLALAVNGEIYNHRELKAELLQPYAFQTGSDCEVINALYREDTPASYLNR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
LNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLRVASELKSLVDDCADAAQFPP
HCCEEEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GHWYDSATGALNRYYERAWRQYGAVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
LDSSLVAAVAARYARHRIEENDITEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHG
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC
FEYTFDEGLDALPDVIRHIETYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGG
CCCCHHHCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCEEE
YLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWGVEPRVPFLDREFLDVAMRMD
EEEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHC
ARFKMIDKTSSGATRMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAQSQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VSDRELAAADRRYPVNPPQTKEAYFYRSLFEQFFPGQAAAETVPGGKSIACSSPTAIAWD
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCEEEEC
ASFAAMADPSGRAIAGVHAQALAS
CCHHCCCCCCCCEEECCHHHHHCC
>Mature Secondary Structure
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDTGAILVHERLAIVDPAG
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCEEEECCCC
GSQPLLSEDGQLALAVNGEIYNHRELKAELLQPYAFQTGSDCEVINALYREDTPASYLNR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
LNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLRVASELKSLVDDCADAAQFPP
HCCEEEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GHWYDSATGALNRYYERAWRQYGAVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
LDSSLVAAVAARYARHRIEENDITEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHG
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC
FEYTFDEGLDALPDVIRHIETYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGG
CCCCHHHCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCEEE
YLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWGVEPRVPFLDREFLDVAMRMD
EEEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHC
ARFKMIDKTSSGATRMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAQSQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VSDRELAAADRRYPVNPPQTKEAYFYRSLFEQFFPGQAAAETVPGGKSIACSSPTAIAWD
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCEEEEC
ASFAAMADPSGRAIAGVHAQALAS
CCHHCCCCCCCCEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]