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The map label for this gene is paaF [C]

Identifier: 21242067

GI number: 21242067

Start: 1517288

End: 1518085

Strand: Direct

Name: paaF [C]

Synonym: XAC1314

Alternate gene names: 21242067

Gene position: 1517288-1518085 (Clockwise)

Preceding gene: 21242066

Following gene: 21242068

Centisome position: 29.32

GC content: 66.79

Gene sequence:

>798_bases
ATGAGCGATTGGGAAGGACGCCTCCATACCGGTCTGCAGGTCGAGCGCGATGGGCACGTCGCCATCGTGACCTTGAGCAA
CCCGCCGGCCAACACCTGGACCGTGCACAGCCTGGCGGCCCTGCGCGACCTGGTGCGCGCGCTGGATGCAGATCGCAGCA
TCTACGCGCTGGTGATCACCGGCGAAGGCGAGAAGTTCTTCAGCGCCGGTGCCGATCTCAAGCAGTTCGCCGATGGCGAC
AAGGCCAATGCACGTGGGGCTGCGCGCCGGTTCGGCGAAGCGTTCGAAGCCTTGAGCGCATTTCGTGGCGTGTCGATTGC
CGCAATCAACGGCTATGCGATGGGGGGCGGGCTGGAATGCGCATTGGCGTGCGATCTGCGCATCGCAGAGCAGCACGCAC
AGCTCGCATTGCCTGAAGCCAGCGTTGGCTTGCTGCCGTGTGCCGGCGGCACCCAGAACCTGCCGCGCCTGGTCGGCGAG
GGCTGGGCCAAGCGCATGATCCTGTTGGGCGAGCGTATCGATGCGGCAACCGCGCAGCGCATCGGCCTGGTGGAAGAGGT
GGTCGGCAAGGGCGAGTCGCGCGCGCTGGCGGTTGCCTGGGCGCAGCGTGCCGGTAAACAGAGCCCGGTCAGTGTGGCGG
CGTGCAAGCAGCTGGTGCAGTCCACGCGCCATGGCACCCACGCAGCGGCATTGATTGCCGAGCGCGAAGCCTTCGTGGAT
CTGTTCGAGCACGCCGATCAGGCCGAAGGCGTGGCCGCATTTCTGGACAAGCGCGCGCCGCAGTGGAGCAACCGCTGA

Upstream 100 bases:

>100_bases
TGCTGCGCGACAGCCGTGTGCACCGCATTCTGGAAGGCACCAACGAGGTGATGCGCATGATCGTGGCGCGTCACTTGCTC
AATGGCGAGGAGCAATTGCG

Downstream 100 bases:

>100_bases
TGGCGCAGGTCAGCGCAACCGACGAGGCAGCGGTGCTGTTCGAGCAGCGTGCGTGCGCCGATGGGCACCGTATCGGCATC
GCTACCTTGAATGCGCCCAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSDWEGRLHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVITGEGEKFFSAGADLKQFADGD
KANARGAARRFGEAFEALSAFRGVSIAAINGYAMGGGLECALACDLRIAEQHAQLALPEASVGLLPCAGGTQNLPRLVGE
GWAKRMILLGERIDAATAQRIGLVEEVVGKGESRALAVAWAQRAGKQSPVSVAACKQLVQSTRHGTHAAALIAEREAFVD
LFEHADQAEGVAAFLDKRAPQWSNR

Sequences:

>Translated_265_residues
MSDWEGRLHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVITGEGEKFFSAGADLKQFADGD
KANARGAARRFGEAFEALSAFRGVSIAAINGYAMGGGLECALACDLRIAEQHAQLALPEASVGLLPCAGGTQNLPRLVGE
GWAKRMILLGERIDAATAQRIGLVEEVVGKGESRALAVAWAQRAGKQSPVSVAACKQLVQSTRHGTHAAALIAEREAFVD
LFEHADQAEGVAAFLDKRAPQWSNR
>Mature_264_residues
SDWEGRLHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVITGEGEKFFSAGADLKQFADGDK
ANARGAARRFGEAFEALSAFRGVSIAAINGYAMGGGLECALACDLRIAEQHAQLALPEASVGLLPCAGGTQNLPRLVGEG
WAKRMILLGERIDAATAQRIGLVEEVVGKGESRALAVAWAQRAGKQSPVSVAACKQLVQSTRHGTHAAALIAEREAFVDL
FEHADQAEGVAAFLDKRAPQWSNR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=32.2709163346614, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI68989263, Length=215, Percent_Identity=37.2093023255814, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI4502327, Length=236, Percent_Identity=29.6610169491525, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI37594471, Length=239, Percent_Identity=27.1966527196653, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594469, Length=239, Percent_Identity=27.1966527196653, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI20127408, Length=190, Percent_Identity=33.6842105263158, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI261878539, Length=109, Percent_Identity=44.954128440367, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=28.7449392712551, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI62530384, Length=225, Percent_Identity=27.1111111111111, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=27.3913043478261, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=27.3913043478261, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI295842266, Length=223, Percent_Identity=26.457399103139, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=27.0916334661355, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694520, Length=174, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI70995211, Length=229, Percent_Identity=29.2576419213974, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=39.2996108949416, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=33.206106870229, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=34.8258706467662, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790281, Length=205, Percent_Identity=31.7073170731707, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=30.1960784313725, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=25.7028112449799, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082183, Length=232, Percent_Identity=25, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=33.7209302325581, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=34.8178137651822, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=28.8793103448276, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=34.4086021505376, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=186, Percent_Identity=36.5591397849462, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=186, Percent_Identity=36.5591397849462, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=186, Percent_Identity=36.5591397849462, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=262, Percent_Identity=27.8625954198473, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=203, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=27.9620853080569, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=26.8482490272374, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=26.5217391304348, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=262, Percent_Identity=25.9541984732824, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=190, Percent_Identity=27.8947368421053, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=190, Percent_Identity=27.8947368421053, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20129971, Length=265, Percent_Identity=33.5849056603774, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24653477, Length=265, Percent_Identity=33.5849056603774, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=29.6577946768061, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=22.7642276422764, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650670, Length=248, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24654903, Length=251, Percent_Identity=24.3027888446215, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357171, Length=239, Percent_Identity=27.6150627615063, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583165, Length=209, Percent_Identity=23.9234449760766, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19921018, Length=187, Percent_Identity=26.2032085561497, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45550169, Length=254, Percent_Identity=24.0157480314961, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28146; Mature: 28015

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDWEGRLHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVIT
CCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
GEGEKFFSAGADLKQFADGDKANARGAARRFGEAFEALSAFRGVSIAAINGYAMGGGLEC
CCCCHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCEE
ALACDLRIAEQHAQLALPEASVGLLPCAGGTQNLPRLVGEGWAKRMILLGERIDAATAQR
EEEECHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IGLVEEVVGKGESRALAVAWAQRAGKQSPVSVAACKQLVQSTRHGTHAAALIAEREAFVD
HHHHHHHHCCCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LFEHADQAEGVAAFLDKRAPQWSNR
HHHHCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDWEGRLHTGLQVERDGHVAIVTLSNPPANTWTVHSLAALRDLVRALDADRSIYALVIT
CCCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
GEGEKFFSAGADLKQFADGDKANARGAARRFGEAFEALSAFRGVSIAAINGYAMGGGLEC
CCCCHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCEE
ALACDLRIAEQHAQLALPEASVGLLPCAGGTQNLPRLVGEGWAKRMILLGERIDAATAQR
EEEECHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IGLVEEVVGKGESRALAVAWAQRAGKQSPVSVAACKQLVQSTRHGTHAAALIAEREAFVD
HHHHHHHHCCCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LFEHADQAEGVAAFLDKRAPQWSNR
HHHHCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA