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The map label for this gene is ahpF [H]

Identifier: 21241676

GI number: 21241676

Start: 1066931

End: 1068523

Strand: Reverse

Name: ahpF [H]

Synonym: XAC0906

Alternate gene names: 21241676

Gene position: 1068523-1066931 (Counterclockwise)

Preceding gene: 21241677

Following gene: 21241675

Centisome position: 20.65

GC content: 64.22

Gene sequence:

>1593_bases
ATGTTGGATGCCAATCTCAAGACCCAGCTGACCGCCTACCTGGAACGGGTGACGCGGCCGATTCAAATCAACGCCTCCAT
CGACGACAGTGCCGGCTCGCGCGAAATGCTCGACCTGCTGGAAGAGTTGGTGCTGTTGTCGGACAAGATCAGCCTGGACA
TCCACCGCGACGACAACCAGCGCAAGCCCTCGTTCGCGCTGACCACGCCCGGCCAGGACATCTCGCTGCGCTTTGCCGGG
CTGCCGATGGGCCATGAGTTCACCTCACTGGTGCTGGCGCTGCTGCAGGTGGGCGGCCACCCGTCCAAGGCCGCCGCCGA
GCTGATCGAGCAGGTGCAGCACCTGGATGGCGATTACCAGTTCGAAACCTATTTCTCGCTGTCGTGCCAGAACTGCCCGG
ACGTGGTGCAGGCGCTGAACCTGGCGGCCGTGCTCAACCCGCGCATCAAGCACGTGGCCATCGATGGCGCCTGGTTCCAG
GAAGAAGTAGAGGCCCGCCAGATCATGTCGGTGCCCACCGTGTACTTGAACGGCGAGCTGTTCGACCAGGGCCGCATGAC
GCTGGAGCAGATCGTCGCCAAGCTGGATACCAATGCCGCCAAGCGCGACGCTGCGAAAATCGCTGCAAAAGAAGCGTTCG
ACGTGCTGGTGGTGGGCGGTGGCCCGGCCGGTTCGGCAGCAGCGGTGTATGCCGCACGCAAGGGCATCCGCACCGGCGTG
GCCGCCGAGCGTTTTGGTGGGCAGGTGCTGGACACCATGTCGATCGAGAACTTCATTTCCGTGCCGGAAACCGAAGGCCC
GAAGATGGCCGCCGCGCTCGAGCAACACGTGCGCCAGTACGACGTGGACATCATGAATCTGCAGCGTGCCGAGCAGCTGA
TCCCGGCCGGTGCCGATGGCCTGATCGAAATCAAGCTGGCCAATGGCGCCTCGCTCAAGAGCAAGACCGTGATCCTGTCC
ACCGGTGCGCGCTGGAGGCAGATGAACGTGCCTGGCGAAGATCAGTACAAGAACAAGGGCGTGGCGTATTGCCCGCACTG
CGATGGCCCGCTGTTCAAGGGCAAGCGCGTGGCGGTGATCGGCGGCGGCAACTCGGGCGTGGAAGCGGCGATCGATCTGG
CCGGCATCGTGGCGCATGTCACGCTGGTGGAATTCGACGACAAGCTGCGCGCCGACGAAGTGCTCCAACGCAAGCTGCGC
AGCCTGCACAACGTACGCATCATCACCAGCGCGCAGACCACTGAAGTACTCGGCGACGGACAGAAGGTCACCGGTCTGGT
CTACAAGGACCGCACCGGCGGCGATATCCAGCACATCGAGCTGGAAGGCGTGTTCGTGCAGATCGGCCTGCTCCCCAACA
CCGAGTTCCTGCGCGGCACGGTGGCGCTGTCGCCGCGCGGCGAAGTCATCGTCGACGACCGTGGCCAGACCGATGTACCC
GGCGTGTTCGCCGCTGGCGACGCCACCACGGTGCCGTACAAGCAGATCGTGATCGCAATGGGCGAAGGTTCCAAGGCGGC
GCTGAGTGCATTCGACCACCTGATCCGCACCAGCGCACCGGCCACTGCCGACAGTGTGGCCCAGGCCGCATAA

Upstream 100 bases:

>100_bases
CCGACCTCGCGCCGGCTGCGGTTCACTCCCTTCCTCCCCGGCGACCTGCCTCAGCGCAGCGACGCCATGCCCTCCCCTTG
CCCATTCCCAGGAGTTCTCC

Downstream 100 bases:

>100_bases
GCAAGCGCCCGCTTAGCGCCCGGCCAACCCGGGCGCTAAGCTTGCAACACGCGCCAGTGGTTCCCCGTCACCGCAATGAA
GGCTGCTGTATATGAATCTG

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQRKPSFALTTPGQDISLRFAG
LPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLDGDYQFETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQ
EEVEARQIMSVPTVYLNGELFDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADGLIEIKLANGASLKSKTVILS
TGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLR
SLHNVRIITSAQTTEVLGDGQKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEVIVDDRGQTDVP
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA

Sequences:

>Translated_530_residues
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQRKPSFALTTPGQDISLRFAG
LPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLDGDYQFETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQ
EEVEARQIMSVPTVYLNGELFDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADGLIEIKLANGASLKSKTVILS
TGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLR
SLHNVRIITSAQTTEVLGDGQKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEVIVDDRGQTDVP
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA
>Mature_530_residues
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQRKPSFALTTPGQDISLRFAG
LPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLDGDYQFETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQ
EEVEARQIMSVPTVYLNGELFDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADGLIEIKLANGASLKSKTVILS
TGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLR
SLHNVRIITSAQTTEVLGDGQKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEVIVDDRGQTDVP
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=521, Percent_Identity=64.8752399232246, Blast_Score=693, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=314, Percent_Identity=33.7579617834395, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6321898, Length=340, Percent_Identity=29.7058823529412, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320560, Length=313, Percent_Identity=30.6709265175719, Blast_Score=143, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56910; Mature: 56910

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQ
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
RKPSFALTTPGQDISLRFAGLPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLDGDYQ
CCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
FETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQEEVEARQIMSVPTVYLNGEL
EEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEECCEE
FDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
ECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCHHCCC
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADG
HHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
LIEIKLANGASLKSKTVILSTGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVI
EEEEEECCCCCCCCCEEEEECCCCEEECCCCCCHHHCCCCEEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLRSLHNVRIITSAQTTEVLGDG
ECCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHCCC
QKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEVIVDDRGQTDVP
CEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCHHHEEEEEECCCCCEEECCCCCCCCC
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA
CEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MLDANLKTQLTAYLERVTRPIQINASIDDSAGSREMLDLLEELVLLSDKISLDIHRDDNQ
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
RKPSFALTTPGQDISLRFAGLPMGHEFTSLVLALLQVGGHPSKAAAELIEQVQHLDGDYQ
CCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
FETYFSLSCQNCPDVVQALNLAAVLNPRIKHVAIDGAWFQEEVEARQIMSVPTVYLNGEL
EEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEECCEE
FDQGRMTLEQIVAKLDTNAAKRDAAKIAAKEAFDVLVVGGGPAGSAAAVYAARKGIRTGV
ECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCHHCCC
AAERFGGQVLDTMSIENFISVPETEGPKMAAALEQHVRQYDVDIMNLQRAEQLIPAGADG
HHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
LIEIKLANGASLKSKTVILSTGARWRQMNVPGEDQYKNKGVAYCPHCDGPLFKGKRVAVI
EEEEEECCCCCCCCCEEEEECCCCEEECCCCCCHHHCCCCEEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLVEFDDKLRADEVLQRKLRSLHNVRIITSAQTTEVLGDG
ECCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHCCC
QKVTGLVYKDRTGGDIQHIELEGVFVQIGLLPNTEFLRGTVALSPRGEVIVDDRGQTDVP
CEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCHHHEEEEEECCCCCEEECCCCCCCCC
GVFAAGDATTVPYKQIVIAMGEGSKAALSAFDHLIRTSAPATADSVAQAA
CEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9190810 [H]