Click here to switch to the map view.

The map label for this gene is flbD [H]

Identifier: 21241615

GI number: 21241615

Start: 1004256

End: 1005383

Strand: Reverse

Name: flbD [H]

Synonym: XAC0845

Alternate gene names: 21241615

Gene position: 1005383-1004256 (Counterclockwise)

Preceding gene: 21241616

Following gene: 77748552

Centisome position: 19.43

GC content: 66.58

Gene sequence:

>1128_bases
GTGTTGAACCGACGCCGCCTCGCGCCCTTCCTCCCCCACGCACGAGCCCGTCCCATGTCCGATGTCCGCTTGCTCACCCT
GCCTGCAGCGCAGCAACACGCGCTCACCGGCAACGCCGTGCGCGCCACCGCGCACGTGTTCGAAGACCCATCTTCCCAGG
CATTGCTGACGCACCTCGAACGGGTTGCGCCAAGCGAAGCCAGCGTGCTGATCGTCGGCGAATCCGGCACCGGCAAAGAG
CTGGTCGCACGTCACATCCATCATCGCAGCGCGCGGGCCCGCCACCCGTTCGTGGCGGTCAACTGCGGCGCCTTCAGCGA
GTCGCTGGTCGATGCCGAACTGTTCGGGCATGAGAAAGGTGCGTTCACCGGCGCCCTGAGCGCCAAGGCCGGCTGGTTCG
AGGAGGCCAACGGCGGCACCTTGTTTCTGGATGAAATCGGCGACCTGCCGATGCCGATCCAGGTCAAGCTGCTGCGCGTA
CTGCAGGAGCGCGAAGTGGTACGGCTGGGCTCGCGCCGCAGCGTGCCGATCGATGTGCGCGTACTGGCCGCCACCAACGT
GCCGCTGGACCACGCCATCCAGCATGGGTATTTCCGCCAGGACCTGTTCTATCGCCTCAACGTGGTGGGCGTGGAGCTCA
AGCCGCTGCGCGAACGTCCCGGCGACATTCCGCCGCTGATCCGCCACTTCGTCGATATCTACAGCCAGCGCCTGGGACAC
GGCCGCGTGACCATCAGCGCGGATGCCGAGCACCTGTTGGTCGAGTACCCGTGGCCAGGCAATATCCGCGAGCTGGAGAA
CGTCATCCACCACACCCTGCTGATCCACCGCGACGGCGTGGTGCGCGCCGAAGACATCCGTCTGTCGCAACTGCGCTTGC
CAGGCCAGGCCGGCGGCGACCCCCACGAAGACGGCCAGGCCCTGCTCGAACGCGCCTTCGACAGCCTGTTCGAAAGCGCC
GGTGGCGCGCTGCATGCGACGGTGGAAGATCAACTGTTCCGTGCCGCGTATCGGCACTGCCACCACAACCAGGTCAAGAC
TGCCGCGCTGCTGGGACTGAGCCGCAACATCGTGCGTGCGCGGCTGATCGAACTGGGCGAGCTGGTGGTGAACAAACGCC
CGGGGTAG

Upstream 100 bases:

>100_bases
GGCGAATGGGCGCTGCGGCAACAGCCACCCACTCCCAGTTTCTATTCCTGAGCCCGACGATGCGTCACCTGCATTTCCGA
CTCGGACCTCGCGTCCCGCC

Downstream 100 bases:

>100_bases
TCACGCCTGGCTGCTTGCGAGACCACGCTGCGCATAGCCGGCTCACACGGCGCGCTGCGAGGGTGCGGTCACCGCAGACG
CAGCGCGCTTGGATCGTTCA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MLNRRRLAPFLPHARARPMSDVRLLTLPAAQQHALTGNAVRATAHVFEDPSSQALLTHLERVAPSEASVLIVGESGTGKE
LVARHIHHRSARARHPFVAVNCGAFSESLVDAELFGHEKGAFTGALSAKAGWFEEANGGTLFLDEIGDLPMPIQVKLLRV
LQEREVVRLGSRRSVPIDVRVLAATNVPLDHAIQHGYFRQDLFYRLNVVGVELKPLRERPGDIPPLIRHFVDIYSQRLGH
GRVTISADAEHLLVEYPWPGNIRELENVIHHTLLIHRDGVVRAEDIRLSQLRLPGQAGGDPHEDGQALLERAFDSLFESA
GGALHATVEDQLFRAAYRHCHHNQVKTAALLGLSRNIVRARLIELGELVVNKRPG

Sequences:

>Translated_375_residues
MLNRRRLAPFLPHARARPMSDVRLLTLPAAQQHALTGNAVRATAHVFEDPSSQALLTHLERVAPSEASVLIVGESGTGKE
LVARHIHHRSARARHPFVAVNCGAFSESLVDAELFGHEKGAFTGALSAKAGWFEEANGGTLFLDEIGDLPMPIQVKLLRV
LQEREVVRLGSRRSVPIDVRVLAATNVPLDHAIQHGYFRQDLFYRLNVVGVELKPLRERPGDIPPLIRHFVDIYSQRLGH
GRVTISADAEHLLVEYPWPGNIRELENVIHHTLLIHRDGVVRAEDIRLSQLRLPGQAGGDPHEDGQALLERAFDSLFESA
GGALHATVEDQLFRAAYRHCHHNQVKTAALLGLSRNIVRARLIELGELVVNKRPG
>Mature_375_residues
MLNRRRLAPFLPHARARPMSDVRLLTLPAAQQHALTGNAVRATAHVFEDPSSQALLTHLERVAPSEASVLIVGESGTGKE
LVARHIHHRSARARHPFVAVNCGAFSESLVDAELFGHEKGAFTGALSAKAGWFEEANGGTLFLDEIGDLPMPIQVKLLRV
LQEREVVRLGSRRSVPIDVRVLAATNVPLDHAIQHGYFRQDLFYRLNVVGVELKPLRERPGDIPPLIRHFVDIYSQRLGH
GRVTISADAEHLLVEYPWPGNIRELENVIHHTLLIHRDGVVRAEDIRLSQLRLPGQAGGDPHEDGQALLERAFDSLFESA
GGALHATVEDQLFRAAYRHCHHNQVKTAALLGLSRNIVRARLIELGELVVNKRPG

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=317, Percent_Identity=42.9022082018927, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1790437, Length=337, Percent_Identity=43.026706231454, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=51.7241379310345, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1790299, Length=338, Percent_Identity=41.1242603550296, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1789233, Length=299, Percent_Identity=42.4749163879599, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789087, Length=249, Percent_Identity=47.3895582329317, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1788905, Length=238, Percent_Identity=49.5798319327731, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1786524, Length=267, Percent_Identity=47.565543071161, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082152, Length=251, Percent_Identity=48.605577689243, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87081872, Length=309, Percent_Identity=37.8640776699029, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787583, Length=318, Percent_Identity=36.4779874213836, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI87081858, Length=268, Percent_Identity=33.5820895522388, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=35.9649122807018, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 41543; Mature: 41543

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRRRLAPFLPHARARPMSDVRLLTLPAAQQHALTGNAVRATAHVFEDPSSQALLTHLE
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHH
RVAPSEASVLIVGESGTGKELVARHIHHRSARARHPFVAVNCGAFSESLVDAELFGHEKG
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCC
AFTGALSAKAGWFEEANGGTLFLDEIGDLPMPIQVKLLRVLQEREVVRLGSRRSVPIDVR
CEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
VLAATNVPLDHAIQHGYFRQDLFYRLNVVGVELKPLRERPGDIPPLIRHFVDIYSQRLGH
EEEECCCCHHHHHHCCHHHHHHHEEEEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCC
GRVTISADAEHLLVEYPWPGNIRELENVIHHTLLIHRDGVVRAEDIRLSQLRLPGQAGGD
CEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHEEECCCCEEECCCEEEHEECCCCCCCC
PHEDGQALLERAFDSLFESAGGALHATVEDQLFRAAYRHCHHNQVKTAALLGLSRNIVRA
CCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RLIELGELVVNKRPG
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNRRRLAPFLPHARARPMSDVRLLTLPAAQQHALTGNAVRATAHVFEDPSSQALLTHLE
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHH
RVAPSEASVLIVGESGTGKELVARHIHHRSARARHPFVAVNCGAFSESLVDAELFGHEKG
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCC
AFTGALSAKAGWFEEANGGTLFLDEIGDLPMPIQVKLLRVLQEREVVRLGSRRSVPIDVR
CEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
VLAATNVPLDHAIQHGYFRQDLFYRLNVVGVELKPLRERPGDIPPLIRHFVDIYSQRLGH
EEEECCCCHHHHHHCCHHHHHHHEEEEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCC
GRVTISADAEHLLVEYPWPGNIRELENVIHHTLLIHRDGVVRAEDIRLSQLRLPGQAGGD
CEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHEEECCCCEEECCCEEEHEECCCCCCCC
PHEDGQALLERAFDSLFESAGGALHATVEDQLFRAAYRHCHHNQVKTAALLGLSRNIVRA
CCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RLIELGELVVNKRPG
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]