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The map label for this gene is dsbA [H]

Identifier: 21241494

GI number: 21241494

Start: 862801

End: 863451

Strand: Reverse

Name: dsbA [H]

Synonym: XAC0723

Alternate gene names: 21241494

Gene position: 863451-862801 (Counterclockwise)

Preceding gene: 21241495

Following gene: 21241493

Centisome position: 16.68

GC content: 66.05

Gene sequence:

>651_bases
ATGAAATTGCTTTCCCGTCTGCCCCTGCTGTTGTTGTTGCTGTTGCCGCTGGTGGCCTGCGCCGCCGACAAGAACGCCGC
GCCGGTCGAAGGCGAGGACTACACCCTGATCGACGGCGGCCAGCCGTACGCCCCGCTTGCCGGCAAGGTCGAAGTGACCG
AAGTCTTCGGCTACACCTGTCCGCACTGCGCGCATTTCGAACCGGTGCTGGAAGCCTGGGTGGCCAAGCAGCCGGCGTAT
GTGCGCTTCACCCCGGTGCCGGCGGCCTTTGGTGGCTTCTGGGACGCGTTCGCACGCGCCTACTTCGCCGCCGACATCCT
GGGCGTGGCCAAGCGCAGTCACCGCGCCATGTTCGACGCCATCCATGAAAAGCAGACCGTGCCCACCCAGAACGTGGCGC
CGGAAGAACTGGCCGCGTTCTACGCCAGTTACGGCATCCCGCAGCAGCGCTTCATCGAGACCTACAAGAGCGAGGCGGTG
GATGCCAAGCTCAAGGCCGCGCGCGAGTTCGCGCTGCGCAGCAAGATCCCCGGCACACCGGCCATCATCGTCAACGGCCG
CTACCTGATCGGCGCACGCAATTACCCGGACATGCTGCGCATTGCCGACTACCTGATCGCCCGCGAGCACGCCGCGCCGG
CCAAGCGCTGA

Upstream 100 bases:

>100_bases
TTCGTGAGCTGATCCCTGCCGTCGCCACACCGCTTCACGACGCCGGTCCCGCGACCGGCGTCGTCGTTTACCGCTCGCCC
CTCGCCCCCACGGAGCCCGC

Downstream 100 bases:

>100_bases
ACCGCATAACCGCCAGCGACGGCCGCGCGTGGCATCATGCGCCCCGCGGCCGTCCGGCCCTTTTCTCGCCCCACGCTGGA
GATCCAATCCGATGAAGACC

Product: disulfide oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKLLSRLPLLLLLLLPLVACAADKNAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTCPHCAHFEPVLEAWVAKQPAY
VRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDAIHEKQTVPTQNVAPEELAAFYASYGIPQQRFIETYKSEAV
DAKLKAAREFALRSKIPGTPAIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR

Sequences:

>Translated_216_residues
MKLLSRLPLLLLLLLPLVACAADKNAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTCPHCAHFEPVLEAWVAKQPAY
VRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDAIHEKQTVPTQNVAPEELAAFYASYGIPQQRFIETYKSEAV
DAKLKAAREFALRSKIPGTPAIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR
>Mature_216_residues
MKLLSRLPLLLLLLLPLVACAADKNAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTCPHCAHFEPVLEAWVAKQPAY
VRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDAIHEKQTVPTQNVAPEELAAFYASYGIPQQRFIETYKSEAV
DAKLKAAREFALRSKIPGTPAIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=187, Percent_Identity=25.668449197861, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23792; Mature: 23792

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLSRLPLLLLLLLPLVACAADKNAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTC
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHCCCC
PHCAHFEPVLEAWVAKQPAYVRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDA
CCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHEKQTVPTQNVAPEELAAFYASYGIPQQRFIETYKSEAVDAKLKAAREFALRSKIPGTP
HHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR
EEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLLSRLPLLLLLLLPLVACAADKNAAPVEGEDYTLIDGGQPYAPLAGKVEVTEVFGYTC
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEHHHHHCCCC
PHCAHFEPVLEAWVAKQPAYVRFTPVPAAFGGFWDAFARAYFAADILGVAKRSHRAMFDA
CCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHEKQTVPTQNVAPEELAAFYASYGIPQQRFIETYKSEAVDAKLKAAREFALRSKIPGTP
HHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AIIVNGRYLIGARNYPDMLRIADYLIAREHAAPAKR
EEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10051655 [H]