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The map label for this gene is dsbA [H]

Identifier: 21241493

GI number: 21241493

Start: 861891

End: 862709

Strand: Reverse

Name: dsbA [H]

Synonym: XAC0722

Alternate gene names: 21241493

Gene position: 862709-861891 (Counterclockwise)

Preceding gene: 21241494

Following gene: 21241492

Centisome position: 16.67

GC content: 67.03

Gene sequence:

>819_bases
ATGAAGACCCGCTTCGCCCTGACGCTGATGGCGCTGTTGCCGATCCTGGTCGCCTGCAAGGCGCAGGACGGTTCGTCCGA
CACCGCACCTGCCGCCACCACCCCAGCTGCCGAAACGGCGCCCGCCCCTCCTGCTGTCGCTACCCCGGCCGCAGACCCCG
CCGCTCCGCCTGCAGTCGGCGAAAGCGCCACCACTCCGCCGCCCGCCGCCCCCAGCGCTGCCCCGCGCGCACCGAATGGC
CCCGAGCCGGTCGCCGGCACCGATTATCTGGACATCGACGGTGGCCAGCCCTATCAGCAGGCCGCCGGCAAGATCGAAGT
GGCCGAAGTCTTCGGCTATGTCTGCCCTGCCTGCAACGCGTTCCAGCCGCTGATCGGGCCATGGAAGGCGGGTCTGCCGT
CGGACGTGCATTTCGTCTATGTGCCGGCCATGTTCGGCGGCCCGTGGGATGACTACGGCCGCGCCTTCTATGCTGCCGAG
ACGCTGGGCGTGCAGGAAAAGACCCACGAAGCCTTGTACAAGGCCATCCACGTCGACCAGACCCTCAAGGGCGAGCGCGG
CAAGGATTCGGTGCAGGACATCGCCGCGTTCTACGCCAAGTACGGCGTGGACCAGAAGACCTTCATCGACACCATGAGCA
GCTTCGGCGTCTCGGCCAAGACCAACCGCGCCAAGCAGTTCGCCACCCGCAGCAAGATCACCGGCACCCCGTCGCTGATC
GTCAACGGCAAGTATCTGGTCAAGGGCCAGAGCTTCCCGGACATGCTGCGCATTGCCGATCACCTGATCGCGCGCGAACG
CGCCACGCTGGCCAAGTGA

Upstream 100 bases:

>100_bases
AAGCGCTGAACCGCATAACCGCCAGCGACGGCCGCGCGTGGCATCATGCGCCCCGCGGCCGTCCGGCCCTTTTCTCGCCC
CACGCTGGAGATCCAATCCG

Downstream 100 bases:

>100_bases
GTCGCGCGAACGCAACGTCGTGAACGCTTCGGACTCGCGCACCCTGCGGGTGCTGACCGCCAATATCCAGGCTGGCTCCA
GCACCCGCCGTTACAGCGAC

Product: thiol:disulfide interchange protein

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKTRFALTLMALLPILVACKAQDGSSDTAPAATTPAAETAPAPPAVATPAADPAAPPAVGESATTPPPAAPSAAPRAPNG
PEPVAGTDYLDIDGGQPYQQAAGKIEVAEVFGYVCPACNAFQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYGRAFYAAE
TLGVQEKTHEALYKAIHVDQTLKGERGKDSVQDIAAFYAKYGVDQKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSLI
VNGKYLVKGQSFPDMLRIADHLIARERATLAK

Sequences:

>Translated_272_residues
MKTRFALTLMALLPILVACKAQDGSSDTAPAATTPAAETAPAPPAVATPAADPAAPPAVGESATTPPPAAPSAAPRAPNG
PEPVAGTDYLDIDGGQPYQQAAGKIEVAEVFGYVCPACNAFQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYGRAFYAAE
TLGVQEKTHEALYKAIHVDQTLKGERGKDSVQDIAAFYAKYGVDQKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSLI
VNGKYLVKGQSFPDMLRIADHLIARERATLAK
>Mature_272_residues
MKTRFALTLMALLPILVACKAQDGSSDTAPAATTPAAETAPAPPAVATPAADPAAPPAVGESATTPPPAAPSAAPRAPNG
PEPVAGTDYLDIDGGQPYQQAAGKIEVAEVFGYVCPACNAFQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYGRAFYAAE
TLGVQEKTHEALYKAIHVDQTLKGERGKDSVQDIAAFYAKYGVDQKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSLI
VNGKYLVKGQSFPDMLRIADHLIARERATLAK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 28579; Mature: 28579

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRFALTLMALLPILVACKAQDGSSDTAPAATTPAAETAPAPPAVATPAADPAAPPAVG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ESATTPPPAAPSAAPRAPNGPEPVAGTDYLDIDGGQPYQQAAGKIEVAEVFGYVCPACNA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHCCCEEHHHHHHHHCCCCCC
FQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYGRAFYAAETLGVQEKTHEALYKAIHVDQ
CCHHCCCHHCCCCCCCEEEEEECHHCCCHHHHHHEEHHHHHHCCHHHHHHHHHHHHHHHH
TLKGERGKDSVQDIAAFYAKYGVDQKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSLI
HHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEE
VNGKYLVKGQSFPDMLRIADHLIARERATLAK
ECCEEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTRFALTLMALLPILVACKAQDGSSDTAPAATTPAAETAPAPPAVATPAADPAAPPAVG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ESATTPPPAAPSAAPRAPNGPEPVAGTDYLDIDGGQPYQQAAGKIEVAEVFGYVCPACNA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHCCCEEHHHHHHHHCCCCCC
FQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYGRAFYAAETLGVQEKTHEALYKAIHVDQ
CCHHCCCHHCCCCCCCEEEEEECHHCCCHHHHHHEEHHHHHHCCHHHHHHHHHHHHHHHH
TLKGERGKDSVQDIAAFYAKYGVDQKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSLI
HHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEE
VNGKYLVKGQSFPDMLRIADHLIARERATLAK
ECCEEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10051655 [H]