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The map label for this gene is dosC [H]

Identifier: 21241197

GI number: 21241197

Start: 499340

End: 500968

Strand: Reverse

Name: dosC [H]

Synonym: XAC0424

Alternate gene names: 21241197

Gene position: 500968-499340 (Counterclockwise)

Preceding gene: 21241206

Following gene: 21241195

Centisome position: 9.68

GC content: 67.77

Gene sequence:

>1629_bases
ATGGCAGACCAGCCCGAACACCCAAGCCGCCCCCGTGGCGGGCTGCGCCGCCTGTTGCCCTGGGGCGGCACGGACACTGC
GCGCCCTGCCGCTGCCCTGCCGACGGTGATCAATGGCGAGCGCGGCGGTCACGGCCTGGCCGCGCACCAGCTGGTGCCGG
CCAAGGCCAAGTCCGGCGCACACGACCCGGCTGCCGCCACTGCATTGCTGATACGGCTGTTCTCGCATGCCGCGCATCCC
GAAGCGCTGCTGCTCGCGTTCGCCGAAGGCATCGCCAGCCTGCATGGCGAGCTCGGCGACATGGGCGTGCGCCTGAGCGC
GGCGTATGCGGCCGGCGATTGGCCCGGCTATGGCCGTGCGTTGCGACAACTCATCGACAAATACATCCGCACCATCGACA
TCGGCGATTCGCTGGCCGAAGGCCGCACGGAGGCCGAGCAACTGCGCGACCTGCTGCGCCACGCGCTGGGCAATGCCCTG
GCCACGCTGCTGCAACGCAGCCCCGAGCTGGCCGAAGAGGCGCAAACGCTGGCATCGGCACTGCGCCACTGGCGCCCGGG
ACACGAGCTGATCACGCTGGAACAACGCCTGCGCGAACTCAGCCACCAGATCGGCCTGCGCGCCGAAGATGCCAGCGAAC
AACAGAACCTGTTATTGGGCTTGTTCGACCTATTGCTGGAGAACGTCGGCGAACTCCTCGACGATCGCAGCTGGTTGCAG
GGACAGATCACGGTGGTGCGGCAACTGATTTCCGGCCCGCTGGACGTCAGTTCCATCGAACAGGCACGCGGCACCTTGCG
CGAGGTGACCTACAAGCAGGGTCTGCTCAAGCAGGGGATCGCCGAGTCCAAGACCGCCATGCGCGAAATGATGGTGTCCT
TCGTCGACCGGCTGGACGGCATGGCCACGAGCACTGGCGAATACCACGACCGCGTTGCCGGGTATTCGCAGGCGATCGGC
GAGGCGCGCGGCATTCCCGATCTCAACCGGCTGCTGCAGGATCTGCTGCAGGACACCGCCGGCGTGCAGGCGCAGGCCCT
GGCGGCGCGCGACGAACTGCTCGCGGCACGCCGGCAGGTGGAAGATTCCGAACAGCGCATCGCCTCGCTCGAACAGGAGC
TCAAGGACGTCGCCGGCCTGGTGCGCGAAGACCAACTCACCGGTGCGCTCAACCGGCGCGGCTTCGAAGAACTGTTCCAG
CGTGAGGCTGTGCGCACCCAGCGTAGTGGCCAGCCCTTGTGCGTGGCGATGCTGGACCTGGACGACTTCCGCCGCCTCAA
TGAGACCCACGGCCATGCCGGCGGCGACGCCGCGCTGCGCCATACCGTGGAGGTGGCCAAGGCCGTGCTGCGCACCACCG
ACGCCATCGCCCGCTTCGGCGGCGAGGAATTCGTCCTGCTGTTGCCGGACTCGACCATTTTCGAGGCCAGTGCGGCAGTG
ATCCGTCTGCAACGCGCACTGTCGCAGCGCTCGCTGCTGCATGACGACGTGCGCGTGTTCATCAGCTTCAGCGCCGGCGT
GGCGCTACGCGGGACCGACGAGACCCAGGACGAGGTCATCCGCCGCGCAGACCGCGCCATGTACGACGCCAAGGCCGCCG
GCAAGAACCGGGTGATCAACGCCGATTAG

Upstream 100 bases:

>100_bases
CCTGCAGGTCGTCGGTGCGCGCTATGGCCCGGCTTGCTCAAGCGCCCCTGCTGCGCGCCGATATCGAGCGTTAGCACAAG
ACCTCTCGAGACCCTGGCGC

Downstream 100 bases:

>100_bases
ACCGGCGGCGAACCCCGGTGCGCCCGAGCAATCGCCGCGCGGTGTTTACCGTGCTGGCTTGTCTGGCGGCTCGCCGCGCA
TCAATGCGCGGGCTTGCTCC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MADQPEHPSRPRGGLRRLLPWGGTDTARPAAALPTVINGERGGHGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHP
EALLLAFAEGIASLHGELGDMGVRLSAAYAAGDWPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNAL
ATLLQRSPELAEEAQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQ
GQITVVRQLISGPLDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVSFVDRLDGMATSTGEYHDRVAGYSQAIG
EARGIPDLNRLLQDLLQDTAGVQAQALAARDELLAARRQVEDSEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQ
REAVRTQRSGQPLCVAMLDLDDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAV
IRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAAGKNRVINAD

Sequences:

>Translated_542_residues
MADQPEHPSRPRGGLRRLLPWGGTDTARPAAALPTVINGERGGHGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHP
EALLLAFAEGIASLHGELGDMGVRLSAAYAAGDWPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNAL
ATLLQRSPELAEEAQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQ
GQITVVRQLISGPLDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVSFVDRLDGMATSTGEYHDRVAGYSQAIG
EARGIPDLNRLLQDLLQDTAGVQAQALAARDELLAARRQVEDSEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQ
REAVRTQRSGQPLCVAMLDLDDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAV
IRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAAGKNRVINAD
>Mature_541_residues
ADQPEHPSRPRGGLRRLLPWGGTDTARPAAALPTVINGERGGHGLAAHQLVPAKAKSGAHDPAAATALLIRLFSHAAHPE
ALLLAFAEGIASLHGELGDMGVRLSAAYAAGDWPGYGRALRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALA
TLLQRSPELAEEAQTLASALRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQG
QITVVRQLISGPLDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVSFVDRLDGMATSTGEYHDRVAGYSQAIGE
ARGIPDLNRLLQDLLQDTAGVQAQALAARDELLAARRQVEDSEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQR
EAVRTQRSGQPLCVAMLDLDDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAVI
RLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAAGKNRVINAD

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=231, Percent_Identity=29.004329004329, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=32.5581395348837, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1786584, Length=176, Percent_Identity=30.6818181818182, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=33.7423312883436, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=31.4285714285714, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=30.8571428571429, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=33.5403726708075, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=186, Percent_Identity=31.1827956989247, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=26.6233766233766, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=24.8587570621469, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59197; Mature: 59066

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQPEHPSRPRGGLRRLLPWGGTDTARPAAALPTVINGERGGHGLAAHQLVPAKAKSGA
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCCCCHHHHCCCCHHCCCC
HDPAAATALLIRLFSHAAHPEALLLAFAEGIASLHGELGDMGVRLSAAYAAGDWPGYGRA
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHHH
LRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEEAQTLASA
HHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQ
HHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GQITVVRQLISGPLDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVSFVDRLDG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MATSTGEYHDRVAGYSQAIGEARGIPDLNRLLQDLLQDTAGVQAQALAARDELLAARRQV
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EDSEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQREAVRTQRSGQPLCVAMLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCH
DDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
IRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAAGKNRVIN
HHHHHHHHHHHHHHHHHHHHHEECCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
AD
CC
>Mature Secondary Structure 
ADQPEHPSRPRGGLRRLLPWGGTDTARPAAALPTVINGERGGHGLAAHQLVPAKAKSGA
CCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCCCCHHHHCCCCHHCCCC
HDPAAATALLIRLFSHAAHPEALLLAFAEGIASLHGELGDMGVRLSAAYAAGDWPGYGRA
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHHH
LRQLIDKYIRTIDIGDSLAEGRTEAEQLRDLLRHALGNALATLLQRSPELAEEAQTLASA
HHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRHWRPGHELITLEQRLRELSHQIGLRAEDASEQQNLLLGLFDLLLENVGELLDDRSWLQ
HHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GQITVVRQLISGPLDVSSIEQARGTLREVTYKQGLLKQGIAESKTAMREMMVSFVDRLDG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MATSTGEYHDRVAGYSQAIGEARGIPDLNRLLQDLLQDTAGVQAQALAARDELLAARRQV
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EDSEQRIASLEQELKDVAGLVREDQLTGALNRRGFEELFQREAVRTQRSGQPLCVAMLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCH
DDFRRLNETHGHAGGDAALRHTVEVAKAVLRTTDAIARFGGEEFVLLLPDSTIFEASAAV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
IRLQRALSQRSLLHDDVRVFISFSAGVALRGTDETQDEVIRRADRAMYDAKAAGKNRVIN
HHHHHHHHHHHHHHHHHHHHHEECCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
AD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]