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The map label for this gene is glpF [H]

Identifier: 21241133

GI number: 21241133

Start: 428455

End: 429276

Strand: Reverse

Name: glpF [H]

Synonym: XAC0359

Alternate gene names: 21241133

Gene position: 429276-428455 (Counterclockwise)

Preceding gene: 21241134

Following gene: 21241132

Centisome position: 8.29

GC content: 64.6

Gene sequence:

>822_bases
ATGAGCCGGCAACTCGTGGGTGAGTTGATCTCCGAAGCGGTCGCGATGTTCATCATCATCGCGCTCGGCGATTCGGTGGC
GGCGATGTACATCCTGTACGACCCCAGCCCCTATCAGAACGCCTATTGGGGCGTGTGTATCGCCTGGGGCTTGGCGGTGA
CGCTGGCGATCTACGTCACGGGCTCGGTGTCCGGTACCCACGCCAATCCGGCGGTCACGCTGGCGCTGGCGCTGTTCCGC
GGCTTTTCCTGGAAGAAGGTGCTCCCCTACTGGGTTGCCCAGGTCATCGGCGCGTTCCTGGGCGCAGCCATCGTCTATCA
GCTGTATGGGCCGGTAATCGATCACTTCAACCAGCTGCATCAACTCACGCGTGAGGCTGGTGGCGCGGCGGGCGTGTTCT
TCACCGCACCGGGGCTGGCGGTGACGCCGATGCATGCGCTGGTCGATCAGATCATCATCACGGCGTTCCTGATCTTCGGC
ATCTTCGCCATCACCGAGCAGTTCAACGAGGCCGCACCGACTGCCAACTCCGGCGCGTTGATGATCGGCTTGTTGGTCGC
CACGCTGGGCGCGTCGATGGGCTATCTGGAAGCCTGGGCGATGAACCCGGCACGCGATTTCGGCCCGCGCCTGTTCGCGT
ACTTCGCCGGCTGGGGCGAGGCCGCCTTGCCGGCCAAGGACAATTATTGGTGGATCCCGATCGTTGGCCCGTTGATCGGT
GGTCCGATCGGCGCGCTGGCTTACCAGTGGCTGATCCTGCCGTTCCTGCCGGCGCGGGTGCGCCATCTGCAGGGCGGGCC
CAAGCCGCTCGATCCGCTGTGA

Upstream 100 bases:

>100_bases
CGTGGAGGGCACCCCAGATGAGCATCACCAACCACCGCTGCGCGCGAGCAAGCGCGCTGCACGCTGTTATCCGCATCATT
GACCTTGCGGGCATGCACGC

Downstream 100 bases:

>100_bases
TGGTGCTGCGCCAAGCCAACAATCACTCCGAAAGTGGGTGAGGTCACCTGTGGCCAGCCCCTATTGGCCACGCGTCGTCA
CCCGCCTGAACCGACCTTTC

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVTGSVSGTHANPAVTLALALFR
GFSWKKVLPYWVAQVIGAFLGAAIVYQLYGPVIDHFNQLHQLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFG
IFAITEQFNEAAPTANSGALMIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIG
GPIGALAYQWLILPFLPARVRHLQGGPKPLDPL

Sequences:

>Translated_273_residues
MSRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVTGSVSGTHANPAVTLALALFR
GFSWKKVLPYWVAQVIGAFLGAAIVYQLYGPVIDHFNQLHQLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFG
IFAITEQFNEAAPTANSGALMIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIG
GPIGALAYQWLILPFLPARVRHLQGGPKPLDPL
>Mature_272_residues
SRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVTGSVSGTHANPAVTLALALFRG
FSWKKVLPYWVAQVIGAFLGAAIVYQLYGPVIDHFNQLHQLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFGI
FAITEQFNEAAPTANSGALMIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIGG
PIGALAYQWLILPFLPARVRHLQGGPKPLDPL

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=249, Percent_Identity=38.5542168674699, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI157266307, Length=256, Percent_Identity=39.84375, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4502187, Length=265, Percent_Identity=32.8301886792453, Blast_Score=148, Evalue=4e-36,
Organism=Homo sapiens, GI22538420, Length=269, Percent_Identity=32.3420074349442, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI310133356, Length=180, Percent_Identity=32.7777777777778, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI310114181, Length=180, Percent_Identity=32.7777777777778, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI4755125, Length=261, Percent_Identity=29.8850574712644, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4502181, Length=261, Percent_Identity=29.8850574712644, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI6912506, Length=231, Percent_Identity=31.1688311688312, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4502183, Length=231, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37694062, Length=267, Percent_Identity=28.4644194756554, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI86792455, Length=211, Percent_Identity=30.3317535545024, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4502179, Length=259, Percent_Identity=28.957528957529, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI45446752, Length=169, Percent_Identity=36.6863905325444, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790362, Length=264, Percent_Identity=41.2878787878788, Blast_Score=178, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17544068, Length=254, Percent_Identity=36.2204724409449, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71992966, Length=261, Percent_Identity=36.3984674329502, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71994009, Length=259, Percent_Identity=30.1158301158301, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531429, Length=276, Percent_Identity=31.1594202898551, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17531431, Length=252, Percent_Identity=32.1428571428571, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71992961, Length=257, Percent_Identity=32.6848249027237, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI32564052, Length=247, Percent_Identity=28.7449392712551, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533613, Length=247, Percent_Identity=28.7449392712551, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=254, Percent_Identity=29.1338582677165, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=183, Percent_Identity=40.9836065573771, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322985, Length=217, Percent_Identity=33.1797235023041, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45550503, Length=249, Percent_Identity=29.3172690763052, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24762342, Length=248, Percent_Identity=31.4516129032258, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24762344, Length=212, Percent_Identity=30.188679245283, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24762346, Length=212, Percent_Identity=30.188679245283, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24762348, Length=212, Percent_Identity=30.188679245283, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20130305, Length=212, Percent_Identity=30.188679245283, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29281; Mature: 29149

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVT
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GSVSGTHANPAVTLALALFRGFSWKKVLPYWVAQVIGAFLGAAIVYQLYGPVIDHFNQLH
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFGIFAITEQFNEAAPTANSGAL
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIG
HHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHC
GPIGALAYQWLILPFLPARVRHLQGGPKPLDPL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SRQLVGELISEAVAMFIIIALGDSVAAMYILYDPSPYQNAYWGVCIAWGLAVTLAIYVT
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GSVSGTHANPAVTLALALFRGFSWKKVLPYWVAQVIGAFLGAAIVYQLYGPVIDHFNQLH
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLTREAGGAAGVFFTAPGLAVTPMHALVDQIIITAFLIFGIFAITEQFNEAAPTANSGAL
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MIGLLVATLGASMGYLEAWAMNPARDFGPRLFAYFAGWGEAALPAKDNYWWIPIVGPLIG
HHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHC
GPIGALAYQWLILPFLPARVRHLQGGPKPLDPL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]