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The map label for this gene is glpK [H]

Identifier: 21241132

GI number: 21241132

Start: 426815

End: 428332

Strand: Reverse

Name: glpK [H]

Synonym: XAC0358

Alternate gene names: 21241132

Gene position: 428332-426815 (Counterclockwise)

Preceding gene: 21241133

Following gene: 21241129

Centisome position: 8.28

GC content: 62.78

Gene sequence:

>1518_bases
TTGTTCGAGGAATCTTCGATGGAAAAGCAGTACGTCCTTGCGATCGATCAAGGCACCACCAGCTCACGCGCGATGTTGTT
CGACCGCCAGGGCAAGGTGGCCGGCGTGGCGCAGCGCGAATTCGGCCAGATCTTCCCGCAGCCTGGCTGGGTGGAACACA
ACCCGCGCGAGATCATGACCAGCGTCTATACCACCATCACCGAGCTGCTCAACAACGCGCAGATCGATGCGCGCGCGATC
GCCGGGATCGGTATCACCAACCAGCGCGAGACCGCAGTGGTGTGGGACAAGGCCACCGGCCAGCCGATCTACAACGCCAT
CGTGTGGCAATCGCGGCAGACCAAGGACATCTGCGCCCAGCTCAAGGAAGCCGGGCATGAGCAGATGGTGCGCGACAAGA
CCGGTTTGCTGATCGATGCGTACTTTTCCGGCACCAAGGTCAAGTGGATTCTCGACCATGTCGAAGGCGCGCGCGAACGC
GCGCAGAAAGGCGAACTGGCGTTCGGCACCATCGACAGCTGGCTGATCTGGAATCTCACCGGCGGCAAGGTGCACGTCAC
CGACTACACCAATGCCTCGCGCACCATGATGTTCAACATCCACACGCTTCAGTGGGATGCTGAACTGCTGGCGATGTTGG
ACGTCCCCGCGCAGATGCTGCCGGAAGTGCGTTCGTCCAGCGAGGTGTATGGCATGACCCAGACCCAGTATTTTTACGGC
GAACAGGTACCCATCGCTGGCATCGCCGGCGACCAGCAGGCCGCGTTGTTCGGCCAGGCCTGTTTCGAGCCGGGCATGGC
GAAAAACACCTACGGCACCGGCTGTTTCATGCTGATGAACACCGGCGACAAGGCGGTGGCATCCAAGGCTGGCTTGTTGA
CCACGATTGCCTGGGGCATCGACGGCAAGGTCGAATACGCATTGGAAGGCGCGATCTTCGTTGCCGGCTCGGTGGTGCAG
TGGCTGCGCGACGGCTTGCGCATGCTGGGCAAGGCCAGCGACTCGCAGGCCTACGCCGAGCGTGCCGGCGACAACGATGG
CGTGTACATCGTGCCGGCCTTCGTTGGCCTGGGCGCGCCGTACTGGCGCAGCGACATTCGCGGCGCCGTGTTCGGGTTGA
CGCGTGGCACCACCAAGGAACACTTCGTGCGTGCGGCAGTCGAATCGATGGCCTACCAGACCCGCGATGTTCTTACCGCA
ATGCAGAGCGATTCCGGCATCGAACTGAAAGAGCTGCGTGCCGACGGCGGCGCCATCGCCAACGACTTCATGGCGCAGTT
CCAGAGCGACATCCTCAATGTGCCGGTACTCCGCCCGGAGGTGGCCGAAACCACTGCACTGGGCGCGGCGTACCTGGCCG
GGCTGGCAACCGGGTTCTGGAGCAGCCGCGAAGAAATCGCCAAGCAATGGGCAGTGGATCGCCGCTTCGAACCGGCCATG
GCCGACGACAAGCGCCAAGCCTTGTACGCCGGCTGGCAGCAAGCAGTGGAAGCGACGATGGGGTTCCGCATCAGCTGA

Upstream 100 bases:

>100_bases
TCACTCCGAAAGTGGGTGAGGTCACCTGTGGCCAGCCCCTATTGGCCACGCGTCGTCACCCGCCTGAACCGACCTTTCTC
GCCGTGCGCCACCGCCGGCA

Downstream 100 bases:

>100_bases
CACTGATGCCCCGATACGACAGCGCCCACCGTGAGGTAGGCGCTTATATTGATTGACCATACGATAGAAAACTGCAGGGC
TACCCAACGCAGCCTGGTGT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MFEESSMEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTITELLNNAQIDARAI
AGIGITNQRETAVVWDKATGQPIYNAIVWQSRQTKDICAQLKEAGHEQMVRDKTGLLIDAYFSGTKVKWILDHVEGARER
AQKGELAFGTIDSWLIWNLTGGKVHVTDYTNASRTMMFNIHTLQWDAELLAMLDVPAQMLPEVRSSSEVYGMTQTQYFYG
EQVPIAGIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEYALEGAIFVAGSVVQ
WLRDGLRMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAPYWRSDIRGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTA
MQSDSGIELKELRADGGAIANDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPAM
ADDKRQALYAGWQQAVEATMGFRIS

Sequences:

>Translated_505_residues
MFEESSMEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTITELLNNAQIDARAI
AGIGITNQRETAVVWDKATGQPIYNAIVWQSRQTKDICAQLKEAGHEQMVRDKTGLLIDAYFSGTKVKWILDHVEGARER
AQKGELAFGTIDSWLIWNLTGGKVHVTDYTNASRTMMFNIHTLQWDAELLAMLDVPAQMLPEVRSSSEVYGMTQTQYFYG
EQVPIAGIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEYALEGAIFVAGSVVQ
WLRDGLRMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAPYWRSDIRGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTA
MQSDSGIELKELRADGGAIANDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPAM
ADDKRQALYAGWQQAVEATMGFRIS
>Mature_505_residues
MFEESSMEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMTSVYTTITELLNNAQIDARAI
AGIGITNQRETAVVWDKATGQPIYNAIVWQSRQTKDICAQLKEAGHEQMVRDKTGLLIDAYFSGTKVKWILDHVEGARER
AQKGELAFGTIDSWLIWNLTGGKVHVTDYTNASRTMMFNIHTLQWDAELLAMLDVPAQMLPEVRSSSEVYGMTQTQYFYG
EQVPIAGIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGIDGKVEYALEGAIFVAGSVVQ
WLRDGLRMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAPYWRSDIRGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTA
MQSDSGIELKELRADGGAIANDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPAM
ADDKRQALYAGWQQAVEATMGFRIS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=46.0159362549801, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=45.6521739130435, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=46.0159362549801, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI41393575, Length=505, Percent_Identity=45.3465346534653, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI88196792, Length=520, Percent_Identity=31.5384615384615, Blast_Score=295, Evalue=8e-80,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=61.8181818181818, Blast_Score=661, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=524, Percent_Identity=23.8549618320611, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787789, Length=530, Percent_Identity=24.3396226415094, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789168, Length=483, Percent_Identity=23.6024844720497, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789987, Length=488, Percent_Identity=23.7704918032787, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=40.6, Blast_Score=380, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71986140, Length=527, Percent_Identity=24.6679316888046, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71986147, Length=268, Percent_Identity=29.8507462686567, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=479, Percent_Identity=38.4133611691023, Blast_Score=303, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24655555, Length=523, Percent_Identity=39.3881453154876, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655560, Length=523, Percent_Identity=39.3881453154876, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655570, Length=523, Percent_Identity=39.3881453154876, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655574, Length=523, Percent_Identity=39.3881453154876, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655565, Length=523, Percent_Identity=39.3881453154876, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17864214, Length=510, Percent_Identity=42.156862745098, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24654573, Length=462, Percent_Identity=41.991341991342, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI19922058, Length=524, Percent_Identity=33.7786259541985, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=34.4195519348269, Blast_Score=279, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24656367, Length=487, Percent_Identity=29.1581108829569, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI21357867, Length=503, Percent_Identity=28.2306163021869, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24656372, Length=326, Percent_Identity=30.9815950920245, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI45552909, Length=292, Percent_Identity=28.4246575342466, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55529; Mature: 55529

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEESSMEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMT
CCCCCCCCCEEEEEEECCCCCCCEEEEECCCCEECHHHHHHHHHCCCCCCCCCCHHHHHH
SVYTTITELLNNAQIDARAIAGIGITNQRETAVVWDKATGQPIYNAIVWQSRQTKDICAQ
HHHHHHHHHHCCCCCCHHHEEECCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHH
LKEAGHEQMVRDKTGLLIDAYFSGTKVKWILDHVEGARERAQKGELAFGTIDSWLIWNLT
HHHCCHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEC
GGKVHVTDYTNASRTMMFNIHTLQWDAELLAMLDVPAQMLPEVRSSSEVYGMTQTQYFYG
CCEEEEEECCCCCEEEEEEEEEEEECHHHHHHHCCCHHHHHHHHCCCCEECCEEEEEECC
EQVPIAGIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGI
CCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHEEEEECC
DGKVEYALEGAIFVAGSVVQWLRDGLRMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAP
CCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEHHHHCCCH
YWRSDIRGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTAMQSDSGIELKELRADGGAIA
HHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHH
NDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPAM
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
ADDKRQALYAGWQQAVEATMGFRIS
CCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFEESSMEKQYVLAIDQGTTSSRAMLFDRQGKVAGVAQREFGQIFPQPGWVEHNPREIMT
CCCCCCCCCEEEEEEECCCCCCCEEEEECCCCEECHHHHHHHHHCCCCCCCCCCHHHHHH
SVYTTITELLNNAQIDARAIAGIGITNQRETAVVWDKATGQPIYNAIVWQSRQTKDICAQ
HHHHHHHHHHCCCCCCHHHEEECCCCCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHH
LKEAGHEQMVRDKTGLLIDAYFSGTKVKWILDHVEGARERAQKGELAFGTIDSWLIWNLT
HHHCCHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEC
GGKVHVTDYTNASRTMMFNIHTLQWDAELLAMLDVPAQMLPEVRSSSEVYGMTQTQYFYG
CCEEEEEECCCCCEEEEEEEEEEEECHHHHHHHCCCHHHHHHHHCCCCEECCEEEEEECC
EQVPIAGIAGDQQAALFGQACFEPGMAKNTYGTGCFMLMNTGDKAVASKAGLLTTIAWGI
CCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHEEEEECC
DGKVEYALEGAIFVAGSVVQWLRDGLRMLGKASDSQAYAERAGDNDGVYIVPAFVGLGAP
CCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEHHHHCCCH
YWRSDIRGAVFGLTRGTTKEHFVRAAVESMAYQTRDVLTAMQSDSGIELKELRADGGAIA
HHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHH
NDFMAQFQSDILNVPVLRPEVAETTALGAAYLAGLATGFWSSREEIAKQWAVDRRFEPAM
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
ADDKRQALYAGWQQAVEATMGFRIS
CCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]