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The map label for this gene is smeA [H]

Identifier: 21241101

GI number: 21241101

Start: 388561

End: 389787

Strand: Direct

Name: smeA [H]

Synonym: XAC0327

Alternate gene names: 21241101

Gene position: 388561-389787 (Clockwise)

Preceding gene: 21241098

Following gene: 21241102

Centisome position: 7.51

GC content: 69.36

Gene sequence:

>1227_bases
ATGCTGCCCGTCGCCGCCCATCGTTTTCGCACTGCGTCCCTGCGCTGGCTGTTGCCCGCCGCCGTGACCACGCTCATGGC
CGCGTGTTCCTCCAGGCAGCCGCCGCAGATGCCGCAGACGCAGGTCGGTGTGCAGACGCTGAAGGTGCAGCGGCTGGCCG
TGGACCAGACCTTGTCCGGCCGCACCGTGGCCTATGTCACCTCGGACGTGCGCCCGCAGGTCGGCGGCATTGTGCGCAAG
CGCCTGTTCACCGAAGGCCAGGACGTCCAGGCCGGGCAAGTGCTGTATGAAATCGATCCGGCCAGCTACCAGGCCGCGTA
CGACACCGCCAAGGGCGATCTGGCGCAAGCCGAGGCGGCGGTGTTGTCGGCCAGGCCCAAGGCGCAGCGCTACCAGACCC
TGGTCGGGCTGGATGCGGTCAGCAAGCAGGACGGCGACGATGCGTTGGCCACGCTGCGCTCGAACGAGGCGGCGGTGGTC
GCGGCCAAGGCGTCGTTGCAGACCGCGCGCATCAATCTCGACTACACCCGCATCACCGCGCCGGTGTCCGGCCGCATCGG
CACCTCCAGCTACACGTCGGGCGCGCTGGTCAGTGCCGGCCAGAGCGAGGTGCTGGCCACCATCAACCAACTCGACCCGA
TCTATGTGGACGTGACCCAGTCCAGCGCACAGCTGCTGCAGTTGCGCCGCCAGCTCGACGCCGGCCAGCTCAAGGCGGTG
GACGGCAAGGCCGAGGTGACGCTGCAACTGGAGGACGGCAGCACCTATGCACACAGCGGCACGTTGGAGGTGGTGGATGC
CGCGGTCGATACCGCCACCGGCACCGTCAAGCTGCGCGCGGTGGTGCCCAATCCCGAGCGCCTGTTGCTGCCGGGCATGT
ATGTCACCGCGAAACTGTCGATGGCCGTGGACGAGCAGGCCATCCTGGTGCCGCAGCAGGCGATCAGCCGCAACAGCAAG
GGCCAGGCGGTGGCGCTGGTGGTCGGCAGCGACAACAAGGTCGCGCAGCGGGTGCTGACCACCGGCGATGCGATCGGCGA
CAAGTGGGTGGTGCGCCAGGGATTGAAGGCTGGCGACAAGGTCATCGTGCAGGGCTTGCAGAAGGCGTCGGTCGGTGCCG
AGGTCAAGGCCGTGGAAGTGACCGCGGCCGCGATGGCCGGCAATGCCGCCGCCACCGCCCCGGCGGCCACGCAGGGCACC
GCCGCGGCGGCGCCCAAGGCCGACTGA

Upstream 100 bases:

>100_bases
TGGAAATCAAGCGCGTTCATGCCGCCGCCGGCAATCTCCAAATTTGCTGCACATTCGCGCCGTAGCGTGGCCACATTCGG
ATAAACCGTGAGTTCGCCGC

Downstream 100 bases:

>100_bases
GGAACCGCCATGGCCCGTTTCTTCATCGACCGTCCCATCTTCGCCTGGGTCATCGCCATCGTGATCACCCTGGCCGGCGC
GCTCTCGATCCTGTCGCTGC

Product: acriflavin resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MLPVAAHRFRTASLRWLLPAAVTTLMAACSSRQPPQMPQTQVGVQTLKVQRLAVDQTLSGRTVAYVTSDVRPQVGGIVRK
RLFTEGQDVQAGQVLYEIDPASYQAAYDTAKGDLAQAEAAVLSARPKAQRYQTLVGLDAVSKQDGDDALATLRSNEAAVV
AAKASLQTARINLDYTRITAPVSGRIGTSSYTSGALVSAGQSEVLATINQLDPIYVDVTQSSAQLLQLRRQLDAGQLKAV
DGKAEVTLQLEDGSTYAHSGTLEVVDAAVDTATGTVKLRAVVPNPERLLLPGMYVTAKLSMAVDEQAILVPQQAISRNSK
GQAVALVVGSDNKVAQRVLTTGDAIGDKWVVRQGLKAGDKVIVQGLQKASVGAEVKAVEVTAAAMAGNAAATAPAATQGT
AAAAPKAD

Sequences:

>Translated_408_residues
MLPVAAHRFRTASLRWLLPAAVTTLMAACSSRQPPQMPQTQVGVQTLKVQRLAVDQTLSGRTVAYVTSDVRPQVGGIVRK
RLFTEGQDVQAGQVLYEIDPASYQAAYDTAKGDLAQAEAAVLSARPKAQRYQTLVGLDAVSKQDGDDALATLRSNEAAVV
AAKASLQTARINLDYTRITAPVSGRIGTSSYTSGALVSAGQSEVLATINQLDPIYVDVTQSSAQLLQLRRQLDAGQLKAV
DGKAEVTLQLEDGSTYAHSGTLEVVDAAVDTATGTVKLRAVVPNPERLLLPGMYVTAKLSMAVDEQAILVPQQAISRNSK
GQAVALVVGSDNKVAQRVLTTGDAIGDKWVVRQGLKAGDKVIVQGLQKASVGAEVKAVEVTAAAMAGNAAATAPAATQGT
AAAAPKAD
>Mature_408_residues
MLPVAAHRFRTASLRWLLPAAVTTLMAACSSRQPPQMPQTQVGVQTLKVQRLAVDQTLSGRTVAYVTSDVRPQVGGIVRK
RLFTEGQDVQAGQVLYEIDPASYQAAYDTAKGDLAQAEAAVLSARPKAQRYQTLVGLDAVSKQDGDDALATLRSNEAAVV
AAKASLQTARINLDYTRITAPVSGRIGTSSYTSGALVSAGQSEVLATINQLDPIYVDVTQSSAQLLQLRRQLDAGQLKAV
DGKAEVTLQLEDGSTYAHSGTLEVVDAAVDTATGTVKLRAVVPNPERLLLPGMYVTAKLSMAVDEQAILVPQQAISRNSK
GQAVALVVGSDNKVAQRVLTTGDAIGDKWVVRQGLKAGDKVIVQGLQKASVGAEVKAVEVTAAAMAGNAAATAPAATQGT
AAAAPKAD

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=378, Percent_Identity=51.5873015873016, Blast_Score=347, Evalue=6e-97,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=49.468085106383, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1789929, Length=338, Percent_Identity=45.8579881656805, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI87082036, Length=340, Percent_Identity=32.0588235294118, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI87081796, Length=322, Percent_Identity=26.3975155279503, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42534; Mature: 42534

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPVAAHRFRTASLRWLLPAAVTTLMAACSSRQPPQMPQTQVGVQTLKVQRLAVDQTLSG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCHHHHHHHHHHHCCCCCC
RTVAYVTSDVRPQVGGIVRKRLFTEGQDVQAGQVLYEIDPASYQAAYDTAKGDLAQAEAA
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHH
VLSARPKAQRYQTLVGLDAVSKQDGDDALATLRSNEAAVVAAKASLQTARINLDYTRITA
HHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCEEEEEEEEEEEEEEC
PVSGRIGTSSYTSGALVSAGQSEVLATINQLDPIYVDVTQSSAQLLQLRRQLDAGQLKAV
CCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEE
DGKAEVTLQLEDGSTYAHSGTLEVVDAAVDTATGTVKLRAVVPNPERLLLPGMYVTAKLS
CCCEEEEEEEECCCEEECCCCCEEEHHHHCCCCCEEEEEEECCCCCEEEECCEEEEEEEE
MAVDEQAILVPQQAISRNSKGQAVALVVGSDNKVAQRVLTTGDAIGDKWVVRQGLKAGDK
EEECCCEEECCHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHCCCCCHH
VIVQGLQKASVGAEVKAVEVTAAAMAGNAAATAPAATQGTAAAAPKAD
HHHHHHHHHCCCCEEEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLPVAAHRFRTASLRWLLPAAVTTLMAACSSRQPPQMPQTQVGVQTLKVQRLAVDQTLSG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCHHHHHHHHHHHCCCCCC
RTVAYVTSDVRPQVGGIVRKRLFTEGQDVQAGQVLYEIDPASYQAAYDTAKGDLAQAEAA
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHH
VLSARPKAQRYQTLVGLDAVSKQDGDDALATLRSNEAAVVAAKASLQTARINLDYTRITA
HHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCEEEEEEEEEEEEEEC
PVSGRIGTSSYTSGALVSAGQSEVLATINQLDPIYVDVTQSSAQLLQLRRQLDAGQLKAV
CCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEE
DGKAEVTLQLEDGSTYAHSGTLEVVDAAVDTATGTVKLRAVVPNPERLLLPGMYVTAKLS
CCCEEEEEEEECCCEEECCCCCEEEHHHHCCCCCEEEEEEECCCCCEEEECCEEEEEEEE
MAVDEQAILVPQQAISRNSKGQAVALVVGSDNKVAQRVLTTGDAIGDKWVVRQGLKAGDK
EEECCCEEECCHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHCCCCCHH
VIVQGLQKASVGAEVKAVEVTAAAMAGNAAATAPAATQGTAAAAPKAD
HHHHHHHHHCCCCEEEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]