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The map label for this gene is purU [H]

Identifier: 21241098

GI number: 21241098

Start: 385337

End: 386188

Strand: Direct

Name: purU [H]

Synonym: XAC0324

Alternate gene names: 21241098

Gene position: 385337-386188 (Clockwise)

Preceding gene: 21241097

Following gene: 21241101

Centisome position: 7.45

GC content: 65.49

Gene sequence:

>852_bases
ATGCGCTCCGACTACATCCTCACCCTGTCCTGCCCCGACCGTACCGGTATCGTCTATCGCGTGACCGGGCTGCTGTTCGA
CCTGGCCTGCAACATCCTCGACGCGCAGCAATTCGGCGACGACGAAAGCGGCCGATTCTTCCTACGCGTGCACTTCGACA
AACCGGCAAAGACCGACATTGCCGCCCTGGAGCAGCGCTTTGCAGTGCTGGCTGACGAATTCCAGATGGCATGGCAACTG
CACGACGCCCGCCGCCGCGCGCGCCTGCTGGTGCTGGTGAGCAAGCAGGGCCATTGCCTCAACGACCTGCTGTTCCGTGC
GCATAGCCGGCAGCTGCGCGTGGACATCGCCGCAGTGGCGTCCAACCACACCGATTTCGCCGCACTGGCCGGCTCCTACG
GCATCGCCTTCCATCATCTGCCCGTGAGCGCGGATACGCGCGCAGAACAGGAAGCGCAGTTGCTCGCCCTGGTCGACGCG
CTGCAGATCGATCTGGTGGTGCTGGCGCGCTACATGCAGATCCTGTCGCCGCAGCTGTGCCGGGCACTGGCCGGGCGCGC
GATCAATATCCATCACAGTTTCCTGCCCAGCTTCAAGGGCGCGCAGCCGTATCACCAGGCGCACGCGCGCGGGGTCAAGA
TCATCGGCGCCACCGCGCACTACGTCACCGAAGATCTGGACGAAGGCCCGATCATCGAGCAGGACGTCGCCCGCGTGGAC
CACGCCATGACCCCGCGCGACCTGGTCCGCCTGGGCAGCGACACCGAATCGCTGGTGCTGGCGCGCGCAGTACGCCGCCA
CGTCGAACACCGCATCGTGCTCAACGGACATCGCACGGTGGTGTTTCGTTGA

Upstream 100 bases:

>100_bases
TCGGCTGGATCGCACGCCGGTTTTCGCACCTGTTCGCACGGCAAAAGTTACCGACGCAAGCACTGCGTCGGTGATGTCGC
GCATTGGCTATCATGCGCCG

Downstream 100 bases:

>100_bases
TACCGGGATTCGGGACTCGGGAATCGCAAAATCAAAGGCTAGGCACTTGCCCCGGACAGTTTCGTTCCGCAAGTCGATGT
GAAACTACAAGCGTCAGACG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRSDYILTLSCPDRTGIVYRVTGLLFDLACNILDAQQFGDDESGRFFLRVHFDKPAKTDIAALEQRFAVLADEFQMAWQL
HDARRRARLLVLVSKQGHCLNDLLFRAHSRQLRVDIAAVASNHTDFAALAGSYGIAFHHLPVSADTRAEQEAQLLALVDA
LQIDLVVLARYMQILSPQLCRALAGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTEDLDEGPIIEQDVARVD
HAMTPRDLVRLGSDTESLVLARAVRRHVEHRIVLNGHRTVVFR

Sequences:

>Translated_283_residues
MRSDYILTLSCPDRTGIVYRVTGLLFDLACNILDAQQFGDDESGRFFLRVHFDKPAKTDIAALEQRFAVLADEFQMAWQL
HDARRRARLLVLVSKQGHCLNDLLFRAHSRQLRVDIAAVASNHTDFAALAGSYGIAFHHLPVSADTRAEQEAQLLALVDA
LQIDLVVLARYMQILSPQLCRALAGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTEDLDEGPIIEQDVARVD
HAMTPRDLVRLGSDTESLVLARAVRRHVEHRIVLNGHRTVVFR
>Mature_283_residues
MRSDYILTLSCPDRTGIVYRVTGLLFDLACNILDAQQFGDDESGRFFLRVHFDKPAKTDIAALEQRFAVLADEFQMAWQL
HDARRRARLLVLVSKQGHCLNDLLFRAHSRQLRVDIAAVASNHTDFAALAGSYGIAFHHLPVSADTRAEQEAQLLALVDA
LQIDLVVLARYMQILSPQLCRALAGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTEDLDEGPIIEQDVARVD
HAMTPRDLVRLGSDTESLVLARAVRRHVEHRIVLNGHRTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=178, Percent_Identity=33.7078651685393, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI209869995, Length=178, Percent_Identity=33.7078651685393, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI209869993, Length=178, Percent_Identity=33.7078651685393, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1787483, Length=282, Percent_Identity=41.8439716312057, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI1788846, Length=150, Percent_Identity=37.3333333333333, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=153, Percent_Identity=38.562091503268, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24582400, Length=157, Percent_Identity=34.3949044585987, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31719; Mature: 31719

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDYILTLSCPDRTGIVYRVTGLLFDLACNILDAQQFGDDESGRFFLRVHFDKPAKTDI
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHH
AALEQRFAVLADEFQMAWQLHDARRRARLLVLVSKQGHCLNDLLFRAHSRQLRVDIAAVA
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEC
SNHTDFAALAGSYGIAFHHLPVSADTRAEQEAQLLALVDALQIDLVVLARYMQILSPQLC
CCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
RALAGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTEDLDEGPIIEQDVARVD
HHHHCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHHHCCCCCCCCHHHHHHHH
HAMTPRDLVRLGSDTESLVLARAVRRHVEHRIVLNGHRTVVFR
HCCCHHHHHHCCCCHHHHHHHHHHHHHHHCEEEEECCEEEEEC
>Mature Secondary Structure
MRSDYILTLSCPDRTGIVYRVTGLLFDLACNILDAQQFGDDESGRFFLRVHFDKPAKTDI
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHH
AALEQRFAVLADEFQMAWQLHDARRRARLLVLVSKQGHCLNDLLFRAHSRQLRVDIAAVA
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEEEC
SNHTDFAALAGSYGIAFHHLPVSADTRAEQEAQLLALVDALQIDLVVLARYMQILSPQLC
CCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
RALAGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTEDLDEGPIIEQDVARVD
HHHHCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHHHCCCCCCCCHHHHHHHH
HAMTPRDLVRLGSDTESLVLARAVRRHVEHRIVLNGHRTVVFR
HCCCHHHHHHCCCCHHHHHHHHHHHHHHHCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]