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The map label for this gene is zraS [H]

Identifier: 21240999

GI number: 21240999

Start: 272967

End: 274409

Strand: Direct

Name: zraS [H]

Synonym: XAC0225

Alternate gene names: 21240999

Gene position: 272967-274409 (Clockwise)

Preceding gene: 21240998

Following gene: 21241000

Centisome position: 5.27

GC content: 68.33

Gene sequence:

>1443_bases
ATGCCCCGTTCGCTGCTCCGGCAACTGTTGCTGATCTGGATGGTGATCGTGGCCGCCTGTGTGGGCGTGGCCACGATGTT
GTACGGCCTGTATCGGCAGACCGAGGGAGTGCGGCTGGAGGAGGCGCGCCAGCAATTGGGCGGGCAATGCGCGCGCATCG
TGCAACGCTATGCCGGGGCAAGCGAGGCAGCGCGTCGCGGCGACGATGGCGCGGGCCTGGCAGCGGTGGTGGTGCAGCTG
GTGCTGGCCGATGCACCAGGTGTCGAAGGCGGTGTCTGGGACCTGCACAAGGGCTTTGTTGCCTACGCGTACCCGACCTA
CGACGGCAGCGATCACAAGACCGATATTCCCGCCGCTGAGTGGGCCAACATCGTCAACGCGGCGCAGCAGAGCGTGACGA
CAAAGCAAGCGGCGCAATACCGTCGCGACGGTCGCCGCGAGGTCCTGCTGATCAGCGCCTGTCCGTTGGCATCCGGCAGC
GCGGCATGGACGATGACGCGGGTGCCGGCCAGCAAGGCCGACGCGTGGCGGCCCCTGGTCGTCGGCATGTTGCTGATCTT
CGCGATGGTGGCGATATCGGCCATTGCGCTGGGCGTGCTGGTGCGGCGCTGGAGCCAGCGCCTGCAGCGTATCCAGCAAG
CGCTGGCCGCCGACACCGAGACGCCGCACATTCCGCCGACCGGGACGCCGGAACTGGACCGGCTGGGCGCGGCGGTCAAC
GACTATGCGCAGCGCAACGCGCAGGCGCTGGCGCAGACGCGCCGGCTGGCCGCCGAACTGTCGCGGCACGAACGGCTTGC
CGCGCTTGGGCGTATGACGGCCACTGTGGCGCATGAAATCCGCAACCCGATTGCCACGATGCGGTTGGCGCTGGAAAACG
AGATCGCGCGCGGCGATGCGACGCCGGACGATGCTGCGCATGGCGAGTTGATGCTGGGGCAGATTCGTCGCCTGGATGGT
GTGGTCGAGAGCCTGCTCGGCATGGTGCAGCCGATCCGTCTGCAGCTGGAGACGGTGGTGGTGCAGGACTGGCTGGCGAC
GCTGCTGGAGCACGGTCCGTTACCGCCCGGCACGTCGCCGCCCCGCGTGCTGCTGCCGGCTGCGCCGTTGCGCTGGACCC
TGGATCCGCAGCAACTGGGGCGCGCGGTGCACAACCTGCTGCGCAATGCCGCGCAGCACGCCGATCCAGGCAGCGAGGTT
ACGCTGCAAGCGGCTGCAGTGGAGGGCATGTTGCAACTTCAGGTCTGCAACCGTGGCGCGCCGATCGCAGAACCGATCGC
CGCACACTTGTTCGAACCGTTCGTCAGTGGCCGCGCCGACGGCAATGGCCTGGGGCTGGCGCTGGTGCGCGAGATCGCGC
GCGCGCATGGCGGACACGCGCGCTACGCACATGCCGACGGGCTGACCCATTTCATTCTGGAGTTGCCATGGCGCGCATCC
TGA

Upstream 100 bases:

>100_bases
AGCGGGCGTGGCGATGAGGTCATCGCGTTGGTAAAGACCAACCTGCGTTAATTCAGTTGCACTCACGCACACTGGCTGTC
CTCTTCAGGCCGCTTCGTCC

Downstream 100 bases:

>100_bases
TCATCGATGACGATGCCGCGTTTCGCACCACCTTGCAGGCCACCTTGCGCTCGCTCGGGCATACGGCGGTCGCCGCCGAG
AATGGGCCGGACGGGCTGGC

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPRSLLRQLLLIWMVIVAACVGVATMLYGLYRQTEGVRLEEARQQLGGQCARIVQRYAGASEAARRGDDGAGLAAVVVQL
VLADAPGVEGGVWDLHKGFVAYAYPTYDGSDHKTDIPAAEWANIVNAAQQSVTTKQAAQYRRDGRREVLLISACPLASGS
AAWTMTRVPASKADAWRPLVVGMLLIFAMVAISAIALGVLVRRWSQRLQRIQQALAADTETPHIPPTGTPELDRLGAAVN
DYAQRNAQALAQTRRLAAELSRHERLAALGRMTATVAHEIRNPIATMRLALENEIARGDATPDDAAHGELMLGQIRRLDG
VVESLLGMVQPIRLQLETVVVQDWLATLLEHGPLPPGTSPPRVLLPAAPLRWTLDPQQLGRAVHNLLRNAAQHADPGSEV
TLQAAAVEGMLQLQVCNRGAPIAEPIAAHLFEPFVSGRADGNGLGLALVREIARAHGGHARYAHADGLTHFILELPWRAS

Sequences:

>Translated_480_residues
MPRSLLRQLLLIWMVIVAACVGVATMLYGLYRQTEGVRLEEARQQLGGQCARIVQRYAGASEAARRGDDGAGLAAVVVQL
VLADAPGVEGGVWDLHKGFVAYAYPTYDGSDHKTDIPAAEWANIVNAAQQSVTTKQAAQYRRDGRREVLLISACPLASGS
AAWTMTRVPASKADAWRPLVVGMLLIFAMVAISAIALGVLVRRWSQRLQRIQQALAADTETPHIPPTGTPELDRLGAAVN
DYAQRNAQALAQTRRLAAELSRHERLAALGRMTATVAHEIRNPIATMRLALENEIARGDATPDDAAHGELMLGQIRRLDG
VVESLLGMVQPIRLQLETVVVQDWLATLLEHGPLPPGTSPPRVLLPAAPLRWTLDPQQLGRAVHNLLRNAAQHADPGSEV
TLQAAAVEGMLQLQVCNRGAPIAEPIAAHLFEPFVSGRADGNGLGLALVREIARAHGGHARYAHADGLTHFILELPWRAS
>Mature_479_residues
PRSLLRQLLLIWMVIVAACVGVATMLYGLYRQTEGVRLEEARQQLGGQCARIVQRYAGASEAARRGDDGAGLAAVVVQLV
LADAPGVEGGVWDLHKGFVAYAYPTYDGSDHKTDIPAAEWANIVNAAQQSVTTKQAAQYRRDGRREVLLISACPLASGSA
AWTMTRVPASKADAWRPLVVGMLLIFAMVAISAIALGVLVRRWSQRLQRIQQALAADTETPHIPPTGTPELDRLGAAVND
YAQRNAQALAQTRRLAAELSRHERLAALGRMTATVAHEIRNPIATMRLALENEIARGDATPDDAAHGELMLGQIRRLDGV
VESLLGMVQPIRLQLETVVVQDWLATLLEHGPLPPGTSPPRVLLPAAPLRWTLDPQQLGRAVHNLLRNAAQHADPGSEVT
LQAAAVEGMLQLQVCNRGAPIAEPIAAHLFEPFVSGRADGNGLGLALVREIARAHGGHARYAHADGLTHFILELPWRAS

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=31.4655172413793, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790300, Length=237, Percent_Identity=29.535864978903, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=28.7610619469027, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786783, Length=319, Percent_Identity=24.7648902821317, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790551, Length=284, Percent_Identity=28.8732394366197, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51751; Mature: 51620

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSLLRQLLLIWMVIVAACVGVATMLYGLYRQTEGVRLEEARQQLGGQCARIVQRYAGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCH
SEAARRGDDGAGLAAVVVQLVLADAPGVEGGVWDLHKGFVAYAYPTYDGSDHKTDIPAAE
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCHHH
WANIVNAAQQSVTTKQAAQYRRDGRREVLLISACPLASGSAAWTMTRVPASKADAWRPLV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHH
VGMLLIFAMVAISAIALGVLVRRWSQRLQRIQQALAADTETPHIPPTGTPELDRLGAAVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
DYAQRNAQALAQTRRLAAELSRHERLAALGRMTATVAHEIRNPIATMRLALENEIARGDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPDDAAHGELMLGQIRRLDGVVESLLGMVQPIRLQLETVVVQDWLATLLEHGPLPPGTSP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PRVLLPAAPLRWTLDPQQLGRAVHNLLRNAAQHADPGSEVTLQAAAVEGMLQLQVCNRGA
CEEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCC
PIAEPIAAHLFEPFVSGRADGNGLGLALVREIARAHGGHARYAHADGLTHFILELPWRAS
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHCCHHHHHHHCCCCCC
>Mature Secondary Structure 
PRSLLRQLLLIWMVIVAACVGVATMLYGLYRQTEGVRLEEARQQLGGQCARIVQRYAGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCH
SEAARRGDDGAGLAAVVVQLVLADAPGVEGGVWDLHKGFVAYAYPTYDGSDHKTDIPAAE
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCHHH
WANIVNAAQQSVTTKQAAQYRRDGRREVLLISACPLASGSAAWTMTRVPASKADAWRPLV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHH
VGMLLIFAMVAISAIALGVLVRRWSQRLQRIQQALAADTETPHIPPTGTPELDRLGAAVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
DYAQRNAQALAQTRRLAAELSRHERLAALGRMTATVAHEIRNPIATMRLALENEIARGDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPDDAAHGELMLGQIRRLDGVVESLLGMVQPIRLQLETVVVQDWLATLLEHGPLPPGTSP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PRVLLPAAPLRWTLDPQQLGRAVHNLLRNAAQHADPGSEVTLQAAAVEGMLQLQVCNRGA
CEEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCC
PIAEPIAAHLFEPFVSGRADGNGLGLALVREIARAHGGHARYAHADGLTHFILELPWRAS
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]