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The map label for this gene is ntrC [H]

Identifier: 21241000

GI number: 21241000

Start: 274394

End: 275740

Strand: Direct

Name: ntrC [H]

Synonym: XAC0226

Alternate gene names: 21241000

Gene position: 274394-275740 (Clockwise)

Preceding gene: 21240999

Following gene: 21241001

Centisome position: 5.3

GC content: 66.96

Gene sequence:

>1347_bases
ATGGCGCGCATCCTGATCATCGATGACGATGCCGCGTTTCGCACCACCTTGCAGGCCACCTTGCGCTCGCTCGGGCATAC
GGCGGTCGCCGCCGAGAATGGGCCGGACGGGCTGGCCCGGTTGAGCGAAGGCGGCATCGACATGGCGTTTGTGGATTTCC
GCATGCCGGGCATGGATGGCATCGCGGTGCTGCGCGCGCGCCTGGACGATGCGCAGGCGCGTCAGGTGCCGCTGGTGATG
CTGACCGCCCATGTGTCCAGCGGCAACACGATCGAGGCGATGACGCTGGGCGCCTTCGATCATCTGGTCAAGCCGGTTGG
TCGTGCCGACATCGTGGAGGTGGTGGAGCGCGCACTGCTCAGTCGCGCCGAGGCGCAGGCTGCAGCGACAGACGCGTTGT
CTGCGCCGGTCGAGGACGACGATGCACTGGTTGGCCACAGCCCGGCGATGCGCACCGTGCACAAGCGCATCGGCTTGGCG
GCCGCCTCTGATCTGCCGGTGCTGATCACCGGGGAAACCGGCACCGGCAAGGAATTGGCGGCACGCGCGTTGCATCGCGC
CAGCCCGCGCGCCAGTGCGCCATTCGCTGCAGTCAACTGCGCGGCCATTCCGCTGGAATTGATGGAGAGCGAGCTGTTCG
GCCATCGCAAGGGTGCGTTTTCCGGCGCCAGCAGCGACCGACGCGGGTTGATTCGCGAAGCCGATGGCGGCACGTTGTTT
CTGGACGAAATCGGCGACATGCCATTGCCGATGCAGGCCAAGCTGCTGCGTTTCCTGCAGGAAGGCGAGGTGACGCCATT
GGGTGGCAGCGGCCCGCAGAAGGTGGATGTGCGGGTGCTGGCCGCCACCCATCGCGACCTGGCGGCCTGCGTGGCCGACG
GGCGCTTTCGTAGCGATCTGCGTTACCGCCTCAATGTGGTGCCGATCGAACTGCCGCCATTGCGCGAGCGTGGCCAGGAC
ATCCTGTTGCTGGCGCAGCACTTCCTGAGCGCCAATGCAGCGCGTGCGCAGAGCCTGTCGCCGGCCGCGCAGGAGCGCCT
GCTCGCGCACCTCTGGCCTGGCAATGTGCGCGAATTGCGCAACGTCATGCAGCGCAGCCAGGTGTTGGTGCGTGGCGCCA
GCATCGATGCGGCGGATCTGGAGGAAGCGCTGGGCGAGGCTGGCGAGGCGCCGCCAAATGGTGCATCGGCGTTGACCGGC
ACCCTGCCCGAAGCGGTGGCGCAGCTGGAAAAACGGATGATCCAGTCGGCGCTGGAACAGAGCCAGGGCAATCGGGCAGA
GGCCGCGCGCCGGTTGGGGATCCACCGGCAATTGCTCTATCGCAAGCTGGAGGAATACGGCTTGTGA

Upstream 100 bases:

>100_bases
GCCTGGGGCTGGCGCTGGTGCGCGAGATCGCGCGCGCGCATGGCGGACACGCGCGCTACGCACATGCCGACGGGCTGACC
CATTTCATTCTGGAGTTGCC

Downstream 100 bases:

>100_bases
CACGCGCGCACGCGTCGATCGCAGACTGCGCGACAGCTGTCCCAAAACCGGACAGGGTGGATGAAATGCAGACGTCTCTC
TTCGGCTGTCGGCCAATGAA

Product: two-component system regulatory protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MARILIIDDDAAFRTTLQATLRSLGHTAVAAENGPDGLARLSEGGIDMAFVDFRMPGMDGIAVLRARLDDAQARQVPLVM
LTAHVSSGNTIEAMTLGAFDHLVKPVGRADIVEVVERALLSRAEAQAAATDALSAPVEDDDALVGHSPAMRTVHKRIGLA
AASDLPVLITGETGTGKELAARALHRASPRASAPFAAVNCAAIPLELMESELFGHRKGAFSGASSDRRGLIREADGGTLF
LDEIGDMPLPMQAKLLRFLQEGEVTPLGGSGPQKVDVRVLAATHRDLAACVADGRFRSDLRYRLNVVPIELPPLRERGQD
ILLLAQHFLSANAARAQSLSPAAQERLLAHLWPGNVRELRNVMQRSQVLVRGASIDAADLEEALGEAGEAPPNGASALTG
TLPEAVAQLEKRMIQSALEQSQGNRAEAARRLGIHRQLLYRKLEEYGL

Sequences:

>Translated_448_residues
MARILIIDDDAAFRTTLQATLRSLGHTAVAAENGPDGLARLSEGGIDMAFVDFRMPGMDGIAVLRARLDDAQARQVPLVM
LTAHVSSGNTIEAMTLGAFDHLVKPVGRADIVEVVERALLSRAEAQAAATDALSAPVEDDDALVGHSPAMRTVHKRIGLA
AASDLPVLITGETGTGKELAARALHRASPRASAPFAAVNCAAIPLELMESELFGHRKGAFSGASSDRRGLIREADGGTLF
LDEIGDMPLPMQAKLLRFLQEGEVTPLGGSGPQKVDVRVLAATHRDLAACVADGRFRSDLRYRLNVVPIELPPLRERGQD
ILLLAQHFLSANAARAQSLSPAAQERLLAHLWPGNVRELRNVMQRSQVLVRGASIDAADLEEALGEAGEAPPNGASALTG
TLPEAVAQLEKRMIQSALEQSQGNRAEAARRLGIHRQLLYRKLEEYGL
>Mature_447_residues
ARILIIDDDAAFRTTLQATLRSLGHTAVAAENGPDGLARLSEGGIDMAFVDFRMPGMDGIAVLRARLDDAQARQVPLVML
TAHVSSGNTIEAMTLGAFDHLVKPVGRADIVEVVERALLSRAEAQAAATDALSAPVEDDDALVGHSPAMRTVHKRIGLAA
ASDLPVLITGETGTGKELAARALHRASPRASAPFAAVNCAAIPLELMESELFGHRKGAFSGASSDRRGLIREADGGTLFL
DEIGDMPLPMQAKLLRFLQEGEVTPLGGSGPQKVDVRVLAATHRDLAACVADGRFRSDLRYRLNVVPIELPPLRERGQDI
LLLAQHFLSANAARAQSLSPAAQERLLAHLWPGNVRELRNVMQRSQVLVRGASIDAADLEEALGEAGEAPPNGASALTGT
LPEAVAQLEKRMIQSALEQSQGNRAEAARRLGIHRQLLYRKLEEYGL

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=472, Percent_Identity=43.4322033898305, Blast_Score=351, Evalue=7e-98,
Organism=Escherichia coli, GI1788550, Length=458, Percent_Identity=43.4497816593886, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1790437, Length=444, Percent_Identity=45.4954954954955, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1788905, Length=451, Percent_Identity=42.5720620842572, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1789233, Length=378, Percent_Identity=41.7989417989418, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI87082117, Length=319, Percent_Identity=47.9623824451411, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=48.2649842271293, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789087, Length=328, Percent_Identity=45.1219512195122, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1786524, Length=325, Percent_Identity=44.9230769230769, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI87081872, Length=312, Percent_Identity=41.9871794871795, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787583, Length=310, Percent_Identity=39.6774193548387, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI87081858, Length=292, Percent_Identity=33.2191780821918, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789828, Length=287, Percent_Identity=33.1010452961672, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788191, Length=116, Percent_Identity=32.7586206896552, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786599, Length=110, Percent_Identity=29.0909090909091, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 47824; Mature: 47693

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARILIIDDDAAFRTTLQATLRSLGHTAVAAENGPDGLARLSEGGIDMAFVDFRMPGMDG
CEEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCH
IAVLRARLDDAQARQVPLVMLTAHVSSGNTIEAMTLGAFDHLVKPVGRADIVEVVERALL
HHHHHHHHCCHHHHCCCEEEEEEECCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHH
SRAEAQAAATDALSAPVEDDDALVGHSPAMRTVHKRIGLAAASDLPVLITGETGTGKELA
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCCCEEEECCCCCCHHHH
ARALHRASPRASAPFAAVNCAAIPLELMESELFGHRKGAFSGASSDRRGLIREADGGTLF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEE
LDEIGDMPLPMQAKLLRFLQEGEVTPLGGSGPQKVDVRVLAATHRDLAACVADGRFRSDL
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCCHHCCC
RYRLNVVPIELPPLRERGQDILLLAQHFLSANAARAQSLSPAAQERLLAHLWPGNVRELR
EEEEEEEEECCCCHHHCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCCHHHHH
NVMQRSQVLVRGASIDAADLEEALGEAGEAPPNGASALTGTLPEAVAQLEKRMIQSALEQ
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHH
SQGNRAEAARRLGIHRQLLYRKLEEYGL
CCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARILIIDDDAAFRTTLQATLRSLGHTAVAAENGPDGLARLSEGGIDMAFVDFRMPGMDG
EEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCH
IAVLRARLDDAQARQVPLVMLTAHVSSGNTIEAMTLGAFDHLVKPVGRADIVEVVERALL
HHHHHHHHCCHHHHCCCEEEEEEECCCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHH
SRAEAQAAATDALSAPVEDDDALVGHSPAMRTVHKRIGLAAASDLPVLITGETGTGKELA
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCCCEEEECCCCCCHHHH
ARALHRASPRASAPFAAVNCAAIPLELMESELFGHRKGAFSGASSDRRGLIREADGGTLF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEE
LDEIGDMPLPMQAKLLRFLQEGEVTPLGGSGPQKVDVRVLAATHRDLAACVADGRFRSDL
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCCHHCCC
RYRLNVVPIELPPLRERGQDILLLAQHFLSANAARAQSLSPAAQERLLAHLWPGNVRELR
EEEEEEEEECCCCHHHCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCCHHHHH
NVMQRSQVLVRGASIDAADLEEALGEAGEAPPNGASALTGTLPEAVAQLEKRMIQSALEQ
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHH
SQGNRAEAARRLGIHRQLLYRKLEEYGL
CCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]