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The map label for this gene is poxB [H]

Identifier: 21240998

GI number: 21240998

Start: 271190

End: 272917

Strand: Direct

Name: poxB [H]

Synonym: XAC0224

Alternate gene names: 21240998

Gene position: 271190-272917 (Clockwise)

Preceding gene: 21240996

Following gene: 21240999

Centisome position: 5.24

GC content: 63.95

Gene sequence:

>1728_bases
GTGGCGAAGAAGAGAACGTTGGCGCGCTTTCTGGCCGAGACGCTGCAGAGCGCCGGGGTCGAGCGGATCTGGGGCGTCAC
CGGCGACAGCCTCAACGGCTTGACCGATGCCTTGCGCGAGATGCAGACCATCGAATGGATGCATGTGCGGCACGAAGAAG
TGGCCGCATTCGCCGCAGGTGCCGAGGCGGCGGTGACCGGCCAGCTGGCGGTGTGTGCGGGCAGTTGCGGGCCGGGCAAT
CTGCACCTGATCAACGGGCTGTTCGATTGCCATCGCAGCCGCCAGCCGGTACTTGCGATTGCCGCGCATATCCCATCGTC
CGAGATCGGGCTCAGCTACTTTCAGGAGACCCACCCGCAAGAGCTGTTTCGCGAGTGCAGTCACTTCGTGGAGATGGTGA
CCAACCCGGCGCAGCTGCCGGAAGTGCTGCATCGGGCGATGCGCACGGCCATCCTGCAGCAAGGCGTGACGGTGGTGGTG
ATTCCCGGCGATATCGCGTTGCTGGAGGTCGACAAACATGTCTCCAGTGCGTGGCCGGCGCTGCGTCAGCCGCGGGTGCT
GCCTGCCGCCGAGGATGTGCGGCAATTGGTCGAGCTGCTGCAGCAGAGCAAGGCAGTGACGCTGTTGTGCGGCAGTGGCT
GCGCCGGCGCGCATGACGCAGTGGTGGCGTTGGCCGACACGTTGGGTGCGCCGGTGGTGCATGCGCTACGCGGCAAGGAA
TACGTGGAGTGGGACAACCCGTTCGATGTCGGCATGACCGGCCTGATCGGTTTCAGTTCCGGTTATCACGCGATGCTCAA
TTGCGACACCTTGCTCATGCTCGGCACGGATTTTCCGTACCGACAGTTCTACCCGAAGGACGCGACCATCGTGCAGGTGG
ATCGCGACCCGAGCGCGCTCGGTCGGCGCACGCCGTTGCAGCTGGGCATTGCCGCCGATGTGGGCGAAACCATCGCAGCG
TTGCTGCCGCAGCTGCAGCGCCGCGAAGAACGTGGCTTCCTGGAAAAATCGCTTGCGCACTACCGCAAGGCGCGCGAAGG
ACTGGACGATCTGGCGGTGCCGGCAGATCCCGGGCAGCCACTGCACCCGCAGTTCGTGACCCGGCTGATCAGCCAGATTG
CCGATCAGGATGCAGTGTTCACCTGCGATGTCGGCACGCCCACGGTGTGGGCCGCCCGCTACCTGCGCATGAACGGCAAG
CGCCGCTTGCTGGGGTCGTTCAATCACGGCTCGATGGCCAACGCGATGCCGCAGGCGCTTGGCGCGCAAGCAGCATTTCC
TGGTCGGCAAGTCATTTCGCTATCGGGCGATGGCGGTTTTGCGATGCTGATGGGCGATTTCATTTCGCTGGCGCAATTGC
ATCTGCCGGTGAAGGTGGTGGTGTTCAACAACGCCTCGCTGGGATTCGTGGCCATGGAGATGAAGGCCGCCGGTTATCTG
GATACCGGTACCGAGCTGCGCAATCCGGATTTCGCGGCGATGAGCAACGCGATGGGCATCCTGGGCATTCGTGTCGATGA
GTCGTCGCAACTGGAGCGTGCATTGCGCCAAGCCTTCGCGCATTCGGGTCCGGCGCTGGTGGATGTGCGGGTCGCCACGC
AGGAGCTGGCGGTGCCGCCGGCGATCAAGCTCGAACAGGCCAAGGGGATGGGCCTGTATCTGCTCAAGGCAGTGATGAGC
GGGCGTGGCGATGAGGTCATCGCGTTGGTAAAGACCAACCTGCGTTAA

Upstream 100 bases:

>100_bases
TTTCCAGCGTTTTACCTGCGCTCGCTAGACAAGCACATTGCACTGACGCAGTCGCTGGGAGCATTGCACGCCTGTTGAAC
ACACCTTGTGGAGCTGCATC

Downstream 100 bases:

>100_bases
TTCAGTTGCACTCACGCACACTGGCTGTCCTCTTCAGGCCGCTTCGTCCATGCCCCGTTCGCTGCTCCGGCAACTGTTGC
TGATCTGGATGGTGATCGTG

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MAKKRTLARFLAETLQSAGVERIWGVTGDSLNGLTDALREMQTIEWMHVRHEEVAAFAAGAEAAVTGQLAVCAGSCGPGN
LHLINGLFDCHRSRQPVLAIAAHIPSSEIGLSYFQETHPQELFRECSHFVEMVTNPAQLPEVLHRAMRTAILQQGVTVVV
IPGDIALLEVDKHVSSAWPALRQPRVLPAAEDVRQLVELLQQSKAVTLLCGSGCAGAHDAVVALADTLGAPVVHALRGKE
YVEWDNPFDVGMTGLIGFSSGYHAMLNCDTLLMLGTDFPYRQFYPKDATIVQVDRDPSALGRRTPLQLGIAADVGETIAA
LLPQLQRREERGFLEKSLAHYRKAREGLDDLAVPADPGQPLHPQFVTRLISQIADQDAVFTCDVGTPTVWAARYLRMNGK
RRLLGSFNHGSMANAMPQALGAQAAFPGRQVISLSGDGGFAMLMGDFISLAQLHLPVKVVVFNNASLGFVAMEMKAAGYL
DTGTELRNPDFAAMSNAMGILGIRVDESSQLERALRQAFAHSGPALVDVRVATQELAVPPAIKLEQAKGMGLYLLKAVMS
GRGDEVIALVKTNLR

Sequences:

>Translated_575_residues
MAKKRTLARFLAETLQSAGVERIWGVTGDSLNGLTDALREMQTIEWMHVRHEEVAAFAAGAEAAVTGQLAVCAGSCGPGN
LHLINGLFDCHRSRQPVLAIAAHIPSSEIGLSYFQETHPQELFRECSHFVEMVTNPAQLPEVLHRAMRTAILQQGVTVVV
IPGDIALLEVDKHVSSAWPALRQPRVLPAAEDVRQLVELLQQSKAVTLLCGSGCAGAHDAVVALADTLGAPVVHALRGKE
YVEWDNPFDVGMTGLIGFSSGYHAMLNCDTLLMLGTDFPYRQFYPKDATIVQVDRDPSALGRRTPLQLGIAADVGETIAA
LLPQLQRREERGFLEKSLAHYRKAREGLDDLAVPADPGQPLHPQFVTRLISQIADQDAVFTCDVGTPTVWAARYLRMNGK
RRLLGSFNHGSMANAMPQALGAQAAFPGRQVISLSGDGGFAMLMGDFISLAQLHLPVKVVVFNNASLGFVAMEMKAAGYL
DTGTELRNPDFAAMSNAMGILGIRVDESSQLERALRQAFAHSGPALVDVRVATQELAVPPAIKLEQAKGMGLYLLKAVMS
GRGDEVIALVKTNLR
>Mature_574_residues
AKKRTLARFLAETLQSAGVERIWGVTGDSLNGLTDALREMQTIEWMHVRHEEVAAFAAGAEAAVTGQLAVCAGSCGPGNL
HLINGLFDCHRSRQPVLAIAAHIPSSEIGLSYFQETHPQELFRECSHFVEMVTNPAQLPEVLHRAMRTAILQQGVTVVVI
PGDIALLEVDKHVSSAWPALRQPRVLPAAEDVRQLVELLQQSKAVTLLCGSGCAGAHDAVVALADTLGAPVVHALRGKEY
VEWDNPFDVGMTGLIGFSSGYHAMLNCDTLLMLGTDFPYRQFYPKDATIVQVDRDPSALGRRTPLQLGIAADVGETIAAL
LPQLQRREERGFLEKSLAHYRKAREGLDDLAVPADPGQPLHPQFVTRLISQIADQDAVFTCDVGTPTVWAARYLRMNGKR
RLLGSFNHGSMANAMPQALGAQAAFPGRQVISLSGDGGFAMLMGDFISLAQLHLPVKVVVFNNASLGFVAMEMKAAGYLD
TGTELRNPDFAAMSNAMGILGIRVDESSQLERALRQAFAHSGPALVDVRVATQELAVPPAIKLEQAKGMGLYLLKAVMSG
RGDEVIALVKTNLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=556, Percent_Identity=22.4820143884892, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1787096, Length=570, Percent_Identity=64.0350877192982, Blast_Score=764, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=27.536231884058, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI87081685, Length=555, Percent_Identity=25.9459459459459, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=25.6227758007117, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788716, Length=548, Percent_Identity=20.8029197080292, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=575, Percent_Identity=24.3478260869565, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=575, Percent_Identity=24.3478260869565, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=600, Percent_Identity=21.8333333333333, Blast_Score=92, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323755, Length=569, Percent_Identity=23.9015817223199, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6320816, Length=544, Percent_Identity=22.7941176470588, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=487, Percent_Identity=22.3819301848049, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922626, Length=556, Percent_Identity=23.7410071942446, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 62053; Mature: 61922

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKRTLARFLAETLQSAGVERIWGVTGDSLNGLTDALREMQTIEWMHVRHEEVAAFAAG
CCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEAAVTGQLAVCAGSCGPGNLHLINGLFDCHRSRQPVLAIAAHIPSSEIGLSYFQETHPQ
CCHHEECCEEEEECCCCCCCEEHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCHH
ELFRECSHFVEMVTNPAQLPEVLHRAMRTAILQQGVTVVVIPGDIALLEVDKHVSSAWPA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEHHHHHHHCHH
LRQPRVLPAAEDVRQLVELLQQSKAVTLLCGSGCAGAHDAVVALADTLGAPVVHALRGKE
HCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHCCCC
YVEWDNPFDVGMTGLIGFSSGYHAMLNCDTLLMLGTDFPYRQFYPKDATIVQVDRDPSAL
EECCCCCCCCCCHHEEECCCCCEEEEECCEEEEECCCCCHHHCCCCCCEEEEECCCHHHH
GRRTPLQLGIAADVGETIAALLPQLQRREERGFLEKSLAHYRKAREGLDDLAVPADPGQP
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
LHPQFVTRLISQIADQDAVFTCDVGTPTVWAARYLRMNGKRRLLGSFNHGSMANAMPQAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
GAQAAFPGRQVISLSGDGGFAMLMGDFISLAQLHLPVKVVVFNNASLGFVAMEMKAAGYL
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHCCCC
DTGTELRNPDFAAMSNAMGILGIRVDESSQLERALRQAFAHSGPALVDVRVATQELAVPP
CCCCCCCCCCHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCC
AIKLEQAKGMGLYLLKAVMSGRGDEVIALVKTNLR
CCEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCC
>Mature Secondary Structure 
AKKRTLARFLAETLQSAGVERIWGVTGDSLNGLTDALREMQTIEWMHVRHEEVAAFAAG
CCHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEAAVTGQLAVCAGSCGPGNLHLINGLFDCHRSRQPVLAIAAHIPSSEIGLSYFQETHPQ
CCHHEECCEEEEECCCCCCCEEHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCHH
ELFRECSHFVEMVTNPAQLPEVLHRAMRTAILQQGVTVVVIPGDIALLEVDKHVSSAWPA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEHHHHHHHCHH
LRQPRVLPAAEDVRQLVELLQQSKAVTLLCGSGCAGAHDAVVALADTLGAPVVHALRGKE
HCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHCCCC
YVEWDNPFDVGMTGLIGFSSGYHAMLNCDTLLMLGTDFPYRQFYPKDATIVQVDRDPSAL
EECCCCCCCCCCHHEEECCCCCEEEEECCEEEEECCCCCHHHCCCCCCEEEEECCCHHHH
GRRTPLQLGIAADVGETIAALLPQLQRREERGFLEKSLAHYRKAREGLDDLAVPADPGQP
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
LHPQFVTRLISQIADQDAVFTCDVGTPTVWAARYLRMNGKRRLLGSFNHGSMANAMPQAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHH
GAQAAFPGRQVISLSGDGGFAMLMGDFISLAQLHLPVKVVVFNNASLGFVAMEMKAAGYL
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHCCCC
DTGTELRNPDFAAMSNAMGILGIRVDESSQLERALRQAFAHSGPALVDVRVATQELAVPP
CCCCCCCCCCHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCC
AIKLEQAKGMGLYLLKAVMSGRGDEVIALVKTNLR
CCEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]