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The map label for this gene is bioB [H]

Identifier: 57238692

GI number: 57238692

Start: 1742370

End: 1743206

Strand: Reverse

Name: bioB [H]

Synonym: CJE1853

Alternate gene names: 57238692

Gene position: 1743206-1742370 (Counterclockwise)

Preceding gene: 57238693

Following gene: 57238691

Centisome position: 98.05

GC content: 36.08

Gene sequence:

>837_bases
ATGCAAATCATGCTTTGTGCTATTTCAAATATAGCGAGTGGAAATTGTTCAGAAGACTGTAAATACTGCACCCAAAGCGC
TCATGTAAAAACAGATATTCAAAAATATCGTCGTAAAGAACTTTCACAAATCGTTTTAGAAGCAAAAATGGCAAAGAAAA
ATGAAGCCTTGGGTTTTTGTTTGGTTACGGCAGGACTTGGGCTTGATGATGAAAAGCTTGAGTATGTTTGCGAAGCTGCC
AAGGCTGTGCAAAAAGAAGCGCCTAATTTGCTTTTAATTGCCTGTAATGGTATGGCAAGCGTGGAACAACTCAAAGAACT
TAAAAAAGCAGGAATTTTTTCTTATAATCACAATCTTGAAAGTTCTAAGGAATTTTTTCCACAAATTTGCACCACACATA
CTTGGGAAAGTAGATTTCAAACCAATTTAAACGCTAAAGAAGCAGGGCTTATGCTTTGTTGTGGTGGAATTTATGGTATG
GGTGAGAGCGAAGAAGATAGGCTAAGTTTTAGAAAATCTTTGCAAGAATTACAACCTTTTTCAACGCCTATTAATTTTTT
TATAGCAAATGAAAATTTAAAACTACAAGTTCCAAGACTTAGTGTTGATGAAGCTTTAAAAATCGTGCGTGACACCAAAG
AGGCTTTACCGCAAAGCGTGGTAATGGTAGCAGGTGGGCGTGAAGTGGTATTACGAGAAAGACAATATGAAATTTTTCAA
GCAGGTGCTGGGGCTATTGTTATAGGGGATTATTTAACCACTAAAGGTGAAGAACCAAGTCAAGATATAATAAAATTAAA
AGAAATGGGATTTACTTTTGCATCAGAGTGTCATTGA

Upstream 100 bases:

>100_bases
TGCGAAGAGTGTGGCGAAACTTTAGTGATAAAAGAGCTTAAAAAAGGTACATTCTTAGAATGTTTAAAGTGTAAAATCAA
AAAAGAAATGAAGGATTAGA

Downstream 100 bases:

>100_bases
TCCACAAGGTTTAATTGATCTAAAAATTCTTATTGTTATAGCTTTATGCTTACTTTTTTCTCCGCATATTGCAAAAATTT
TACGCTTGCCTCTTTCAGCT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MQIMLCAISNIASGNCSEDCKYCTQSAHVKTDIQKYRRKELSQIVLEAKMAKKNEALGFCLVTAGLGLDDEKLEYVCEAA
KAVQKEAPNLLLIACNGMASVEQLKELKKAGIFSYNHNLESSKEFFPQICTTHTWESRFQTNLNAKEAGLMLCCGGIYGM
GESEEDRLSFRKSLQELQPFSTPINFFIANENLKLQVPRLSVDEALKIVRDTKEALPQSVVMVAGGREVVLRERQYEIFQ
AGAGAIVIGDYLTTKGEEPSQDIIKLKEMGFTFASECH

Sequences:

>Translated_278_residues
MQIMLCAISNIASGNCSEDCKYCTQSAHVKTDIQKYRRKELSQIVLEAKMAKKNEALGFCLVTAGLGLDDEKLEYVCEAA
KAVQKEAPNLLLIACNGMASVEQLKELKKAGIFSYNHNLESSKEFFPQICTTHTWESRFQTNLNAKEAGLMLCCGGIYGM
GESEEDRLSFRKSLQELQPFSTPINFFIANENLKLQVPRLSVDEALKIVRDTKEALPQSVVMVAGGREVVLRERQYEIFQ
AGAGAIVIGDYLTTKGEEPSQDIIKLKEMGFTFASECH
>Mature_278_residues
MQIMLCAISNIASGNCSEDCKYCTQSAHVKTDIQKYRRKELSQIVLEAKMAKKNEALGFCLVTAGLGLDDEKLEYVCEAA
KAVQKEAPNLLLIACNGMASVEQLKELKKAGIFSYNHNLESSKEFFPQICTTHTWESRFQTNLNAKEAGLMLCCGGIYGM
GESEEDRLSFRKSLQELQPFSTPINFFIANENLKLQVPRLSVDEALKIVRDTKEALPQSVVMVAGGREVVLRERQYEIFQ
AGAGAIVIGDYLTTKGEEPSQDIIKLKEMGFTFASECH

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=278, Percent_Identity=32.7338129496403, Blast_Score=136, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321725, Length=267, Percent_Identity=32.9588014981273, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 30942; Mature: 30942

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIMLCAISNIASGNCSEDCKYCTQSAHVKTDIQKYRRKELSQIVLEAKMAKKNEALGFC
CEEEEEEHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LVTAGLGLDDEKLEYVCEAAKAVQKEAPNLLLIACNGMASVEQLKELKKAGIFSYNHNLE
EEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEECCCCC
SSKEFFPQICTTHTWESRFQTNLNAKEAGLMLCCGGIYGMGESEEDRLSFRKSLQELQPF
HHHHHHHHHHCCCCHHHHHHCCCCCHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
STPINFFIANENLKLQVPRLSVDEALKIVRDTKEALPQSVVMVAGGREVVLRERQYEIFQ
CCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCHHEEHHHHHHHHHH
AGAGAIVIGDYLTTKGEEPSQDIIKLKEMGFTFASECH
CCCCEEEECCHHHCCCCCCHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MQIMLCAISNIASGNCSEDCKYCTQSAHVKTDIQKYRRKELSQIVLEAKMAKKNEALGFC
CEEEEEEHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LVTAGLGLDDEKLEYVCEAAKAVQKEAPNLLLIACNGMASVEQLKELKKAGIFSYNHNLE
EEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEECCCCC
SSKEFFPQICTTHTWESRFQTNLNAKEAGLMLCCGGIYGMGESEEDRLSFRKSLQELQPF
HHHHHHHHHHCCCCHHHHHHCCCCCHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
STPINFFIANENLKLQVPRLSVDEALKIVRDTKEALPQSVVMVAGGREVVLRERQYEIFQ
CCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCHHEEHHHHHHHHHH
AGAGAIVIGDYLTTKGEEPSQDIIKLKEMGFTFASECH
CCCCEEEECCHHHCCCCCCHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA