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The map label for this gene is topA [H]

Identifier: 57238693

GI number: 57238693

Start: 1743208

End: 1745310

Strand: Reverse

Name: topA [H]

Synonym: CJE1854

Alternate gene names: 57238693

Gene position: 1745310-1743208 (Counterclockwise)

Preceding gene: 57238695

Following gene: 57238692

Centisome position: 98.17

GC content: 33.71

Gene sequence:

>2103_bases
ATGAAGAAAAATTTAATCATAGTAGAATCTCCTGCAAAAGCTAAAACCATAGGAAATTTTTTGGGAAAAGATTATGAAGT
TATCGCTTCTAAAGGACATATTAGAGATTTGCCTAAATCAAGCTTTGGGATTAAAATAGAAGATGATGAATTTATCCCTG
AGTATAGAATCACAAGTGATCATAGCGCCTTAGTTAAAGAACTTAAAAGCAAAGCCAAAGATGCCAAAGAAGTCTATCTT
GCAACGGATGAAGATAGAGAAGGAGAGGCTATAGCCTATCATATTGCAAAAGCTATAGGAAAAGATGAAAACACCTTACC
ACGCATTGTTTTTCATGAAATCACTAAAAGCGCTATAGAAAATGCTTTAAAAAATCCAAGAAAATTAAACATGCACTCTG
TAAATGCTCAGCAAACCAGACGTCTTTTAGATCGCATTGTAGGGTATAAGCTCAGCCCTTTATTAGGACAAAAAATTCAA
AGAGGACTTAGTGCTGGGCGTGTTCAAAGTGCGGCTTTAAAGATCATAGTCGATCGTGAAAAAGAAATTCGTGCCTTTGT
ACCCCTAGAATACTTTAGTATTGATATGATTTTTCAAAAAGATTTAGATGCAGAACTAGTTGAGTTTGATAAAGCCAAGA
TAGAAAAACTTACCATTACCAACAAAGATCGTGCTAAACTTATACTAGAAGCTTGTAAAAATGAAGTTTATAGTATAAGT
GACATTGAAAGCAAAGAAAGAAAAATCGCTCCGCCGCCTCCTTTTATGACTTCAACTTTACAACAAAGTGCCAGCAATCG
CCTTGGTTTTAACCCTAAAAAAACCATGATGATAGCTCAAAAGCTCTATGAAGGAGTAAATACCCATGAAGGCGTGATGG
GGGTTATTACTTATATGAGAACCGATAGTTTAAATTTAGCCAAAGAAGCAGTAGAAAATGCAAGAAAATTCATACAAGCA
AATTTTGGCAAGGACTATCTTCCAAGCAAAGCAAATGTATATACTACAAAGACTAAAGGCGCTCAAGAAGCTCATGAAGC
TATACGCCCTACAAATTTAAGTTTTACTCCAGAAATTGCGGCTAAATTTTTAGACAAAGATGAGTTAAAACTTTACACAC
TTATTTACAATCGCTTTTTAGCCTGTCAGATGAGTCCTGCTATCTCTCAAACTCAAAATGTATTTGTAAAAAATGATAGA
GCAGTTTTTAAAATAAGCGGTAGAAAAATCCTTTTTGATGGATATTATAAAGTTTATGGTGATATGGATAAGGATAAAAT
TTTGCCAAATTTTAAAATAGGCCAAAATTTAAAAGTGCAAAATTTAGAAATGAACTCACATTTTACCGAACCACCTTCAA
GGTATTCAGAAGCAGGACTTGTAAAAAAACTTGAAAGTTTAGGCATAGGTCGCCCTTCTACTTATGCACCAACCATTTCT
ATACTTACAAGCCGTGATTATGTAACAATAGATAAAAAACAACTCATTCCTAGCGATGTTGCTTTTAATGTAACCGAAGT
GCTTGAGAAAAATTTTAGCGATATAGTAGATAGTAAATTTACTTCAAACCTTGAAAATACCCTAGATGAAATCGCAGAAG
ATAAGGCAGATTGGCAAGAAACTTTAAAAGAATTTTACTATCCTTTTATGAGAAAAATCGAAGAAGGAAAAACAAAAATA
GCTAGTCAAAAAACTGTCACAAAACTAGGCGAAAGTTGTCCTGATTGTGGCGGAGAGCTTGCTATTAGAAAAGGGCGTTT
TGGAGAATTTGTAGCGTGTTTAAATTTCCCAAAATGCAAGTATTCAAGAAATTTAAAAAGTGAAAGCAAAAACGAAAGCG
AAAATACAGCCGCAAAAGCTAAAGCTAATGGTACAGGCATTACTTGTCCAAGTTGTCAAAAAGGTGAAATCGTAGAACGC
TTTTCAAAACGTGGTAAATTTTATGGCTGTAGTGCTTATCCAAAATGCAATTTTATAAGCAAATACAAACCAAGTGAAGA
AAAATGCGAAGAGTGTGGCGAAACTTTAGTGATAAAAGAGCTTAAAAAAGGTACATTCTTAGAATGTTTAAAGTGTAAAA
TCAAAAAAGAAATGAAGGATTAG

Upstream 100 bases:

>100_bases
TTTGTTTTTAAAATATTTTAGCTTGATTAAAATATATTTTATAAGTAGAATTTTAAATATTTTTAGCTAAATTCAGCTTT
TTATTTAACAAGGGTGCAAA

Downstream 100 bases:

>100_bases
AATGCAAATCATGCTTTGTGCTATTTCAAATATAGCGAGTGGAAATTGTTCAGAAGACTGTAAATACTGCACCCAAAGCG
CTCATGTAAAAACAGATATT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MKKNLIIVESPAKAKTIGNFLGKDYEVIASKGHIRDLPKSSFGIKIEDDEFIPEYRITSDHSALVKELKSKAKDAKEVYL
ATDEDREGEAIAYHIAKAIGKDENTLPRIVFHEITKSAIENALKNPRKLNMHSVNAQQTRRLLDRIVGYKLSPLLGQKIQ
RGLSAGRVQSAALKIIVDREKEIRAFVPLEYFSIDMIFQKDLDAELVEFDKAKIEKLTITNKDRAKLILEACKNEVYSIS
DIESKERKIAPPPPFMTSTLQQSASNRLGFNPKKTMMIAQKLYEGVNTHEGVMGVITYMRTDSLNLAKEAVENARKFIQA
NFGKDYLPSKANVYTTKTKGAQEAHEAIRPTNLSFTPEIAAKFLDKDELKLYTLIYNRFLACQMSPAISQTQNVFVKNDR
AVFKISGRKILFDGYYKVYGDMDKDKILPNFKIGQNLKVQNLEMNSHFTEPPSRYSEAGLVKKLESLGIGRPSTYAPTIS
ILTSRDYVTIDKKQLIPSDVAFNVTEVLEKNFSDIVDSKFTSNLENTLDEIAEDKADWQETLKEFYYPFMRKIEEGKTKI
ASQKTVTKLGESCPDCGGELAIRKGRFGEFVACLNFPKCKYSRNLKSESKNESENTAAKAKANGTGITCPSCQKGEIVER
FSKRGKFYGCSAYPKCNFISKYKPSEEKCEECGETLVIKELKKGTFLECLKCKIKKEMKD

Sequences:

>Translated_700_residues
MKKNLIIVESPAKAKTIGNFLGKDYEVIASKGHIRDLPKSSFGIKIEDDEFIPEYRITSDHSALVKELKSKAKDAKEVYL
ATDEDREGEAIAYHIAKAIGKDENTLPRIVFHEITKSAIENALKNPRKLNMHSVNAQQTRRLLDRIVGYKLSPLLGQKIQ
RGLSAGRVQSAALKIIVDREKEIRAFVPLEYFSIDMIFQKDLDAELVEFDKAKIEKLTITNKDRAKLILEACKNEVYSIS
DIESKERKIAPPPPFMTSTLQQSASNRLGFNPKKTMMIAQKLYEGVNTHEGVMGVITYMRTDSLNLAKEAVENARKFIQA
NFGKDYLPSKANVYTTKTKGAQEAHEAIRPTNLSFTPEIAAKFLDKDELKLYTLIYNRFLACQMSPAISQTQNVFVKNDR
AVFKISGRKILFDGYYKVYGDMDKDKILPNFKIGQNLKVQNLEMNSHFTEPPSRYSEAGLVKKLESLGIGRPSTYAPTIS
ILTSRDYVTIDKKQLIPSDVAFNVTEVLEKNFSDIVDSKFTSNLENTLDEIAEDKADWQETLKEFYYPFMRKIEEGKTKI
ASQKTVTKLGESCPDCGGELAIRKGRFGEFVACLNFPKCKYSRNLKSESKNESENTAAKAKANGTGITCPSCQKGEIVER
FSKRGKFYGCSAYPKCNFISKYKPSEEKCEECGETLVIKELKKGTFLECLKCKIKKEMKD
>Mature_700_residues
MKKNLIIVESPAKAKTIGNFLGKDYEVIASKGHIRDLPKSSFGIKIEDDEFIPEYRITSDHSALVKELKSKAKDAKEVYL
ATDEDREGEAIAYHIAKAIGKDENTLPRIVFHEITKSAIENALKNPRKLNMHSVNAQQTRRLLDRIVGYKLSPLLGQKIQ
RGLSAGRVQSAALKIIVDREKEIRAFVPLEYFSIDMIFQKDLDAELVEFDKAKIEKLTITNKDRAKLILEACKNEVYSIS
DIESKERKIAPPPPFMTSTLQQSASNRLGFNPKKTMMIAQKLYEGVNTHEGVMGVITYMRTDSLNLAKEAVENARKFIQA
NFGKDYLPSKANVYTTKTKGAQEAHEAIRPTNLSFTPEIAAKFLDKDELKLYTLIYNRFLACQMSPAISQTQNVFVKNDR
AVFKISGRKILFDGYYKVYGDMDKDKILPNFKIGQNLKVQNLEMNSHFTEPPSRYSEAGLVKKLESLGIGRPSTYAPTIS
ILTSRDYVTIDKKQLIPSDVAFNVTEVLEKNFSDIVDSKFTSNLENTLDEIAEDKADWQETLKEFYYPFMRKIEEGKTKI
ASQKTVTKLGESCPDCGGELAIRKGRFGEFVACLNFPKCKYSRNLKSESKNESENTAAKAKANGTGITCPSCQKGEIVER
FSKRGKFYGCSAYPKCNFISKYKPSEEKCEECGETLVIKELKKGTFLECLKCKIKKEMKD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=659, Percent_Identity=25.3414264036419, Blast_Score=163, Evalue=7e-40,
Organism=Homo sapiens, GI4507635, Length=671, Percent_Identity=22.8017883755589, Blast_Score=120, Evalue=5e-27,
Organism=Escherichia coli, GI1787529, Length=750, Percent_Identity=39.2, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1788061, Length=630, Percent_Identity=21.2698412698413, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI48994931, Length=112, Percent_Identity=39.2857142857143, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17555378, Length=513, Percent_Identity=26.7056530214425, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32563869, Length=565, Percent_Identity=24.2477876106195, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=493, Percent_Identity=24.7464503042596, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24585251, Length=649, Percent_Identity=24.8073959938367, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24640096, Length=530, Percent_Identity=22.2641509433962, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79121; Mature: 79121

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNLIIVESPAKAKTIGNFLGKDYEVIASKGHIRDLPKSSFGIKIEDDEFIPEYRITSD
CCCCEEEEECCCHHHHHHHHHCCCCEEHCCCCCCCCCCCCCCCEEECCCCCCCCEEECCC
HSALVKELKSKAKDAKEVYLATDEDREGEAIAYHIAKAIGKDENTLPRIVFHEITKSAIE
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NALKNPRKLNMHSVNAQQTRRLLDRIVGYKLSPLLGQKIQRGLSAGRVQSAALKIIVDRE
HHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHEEEEECCC
KEIRAFVPLEYFSIDMIFQKDLDAELVEFDKAKIEKLTITNKDRAKLILEACKNEVYSIS
CHHHEECCHHHHEEEEHHHCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCEECC
DIESKERKIAPPPPFMTSTLQQSASNRLGFNPKKTMMIAQKLYEGVNTHEGVMGVITYMR
CCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
TDSLNLAKEAVENARKFIQANFGKDYLPSKANVYTTKTKGAQEAHEAIRPTNLSFTPEIA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCHHHH
AKFLDKDELKLYTLIYNRFLACQMSPAISQTQNVFVKNDRAVFKISGRKILFDGYYKVYG
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCEEEEECCCEEEEECHHHHHC
DMDKDKILPNFKIGQNLKVQNLEMNSHFTEPPSRYSEAGLVKKLESLGIGRPSTYAPTIS
CCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEE
ILTSRDYVTIDKKQLIPSDVAFNVTEVLEKNFSDIVDSKFTSNLENTLDEIAEDKADWQE
EEECCCEEEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLKEFYYPFMRKIEEGKTKIASQKTVTKLGESCPDCGGELAIRKGRFGEFVACLNFPKCK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHCCCCCCH
YSRNLKSESKNESENTAAKAKANGTGITCPSCQKGEIVERFSKRGKFYGCSAYPKCNFIS
HHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCC
KYKPSEEKCEECGETLVIKELKKGTFLECLKCKIKKEMKD
CCCCCHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKNLIIVESPAKAKTIGNFLGKDYEVIASKGHIRDLPKSSFGIKIEDDEFIPEYRITSD
CCCCEEEEECCCHHHHHHHHHCCCCEEHCCCCCCCCCCCCCCCEEECCCCCCCCEEECCC
HSALVKELKSKAKDAKEVYLATDEDREGEAIAYHIAKAIGKDENTLPRIVFHEITKSAIE
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NALKNPRKLNMHSVNAQQTRRLLDRIVGYKLSPLLGQKIQRGLSAGRVQSAALKIIVDRE
HHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHEEEEECCC
KEIRAFVPLEYFSIDMIFQKDLDAELVEFDKAKIEKLTITNKDRAKLILEACKNEVYSIS
CHHHEECCHHHHEEEEHHHCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCEECC
DIESKERKIAPPPPFMTSTLQQSASNRLGFNPKKTMMIAQKLYEGVNTHEGVMGVITYMR
CCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
TDSLNLAKEAVENARKFIQANFGKDYLPSKANVYTTKTKGAQEAHEAIRPTNLSFTPEIA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCHHHH
AKFLDKDELKLYTLIYNRFLACQMSPAISQTQNVFVKNDRAVFKISGRKILFDGYYKVYG
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCEEEEECCCEEEEECHHHHHC
DMDKDKILPNFKIGQNLKVQNLEMNSHFTEPPSRYSEAGLVKKLESLGIGRPSTYAPTIS
CCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEE
ILTSRDYVTIDKKQLIPSDVAFNVTEVLEKNFSDIVDSKFTSNLENTLDEIAEDKADWQE
EEECCCEEEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLKEFYYPFMRKIEEGKTKIASQKTVTKLGESCPDCGGELAIRKGRFGEFVACLNFPKCK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHCCCCCCH
YSRNLKSESKNESENTAAKAKANGTGITCPSCQKGEIVERFSKRGKFYGCSAYPKCNFIS
HHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCC
KYKPSEEKCEECGETLVIKELKKGTFLECLKCKIKKEMKD
CCCCCHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]