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The map label for this gene is carA

Identifier: 57238517

GI number: 57238517

Start: 1566959

End: 1568077

Strand: Reverse

Name: carA

Synonym: CJE1667

Alternate gene names: 57238517

Gene position: 1568077-1566959 (Counterclockwise)

Preceding gene: 57238518

Following gene: 57238516

Centisome position: 88.2

GC content: 30.21

Gene sequence:

>1119_bases
ATGAAGGCTTATATTTATTTAGAAAATGATATTTTTTTAAGTGCTAAGGCTTTTGGAAAGAGCGGTACTTTTTTTGGAGA
GCTTGTATTTAACACTTCTTTAACAGGGTATCAAGAAATTATTTCAGATCCTTCTTATGCAGGGCAATTTATCGTATTTT
CCATGCCAGAAATTGGTATAGTGGGAACCAATGAAAATGATAATGAAAGCAAGGAAATTTTTGCAAGTGGTGTTTTAATG
CGTGAATTAAGTTCCAGTTTTTCAAATTTTCGCGCTAAAGAAAGTCTGCAAGATTATCTTGAAAAACATGGAAAAATAGG
AATTTATGAACTTGATACGAGATATCTAGTAAAAATGATAAGAAATAATGGCAATTTAAGAGCTGTAATTTCAACTGAAA
TTTCAAATAAAGAAGATTTAAAAATCGCCTTAGAAAAATCAGCAAAAATTGATGAAGTAAATTTTGTAAAAGAAGTTAGC
ACTAAGAAAAATTATTCTCATAAACAAGGCGTTTGGAACGCTAGTTTTCAAAAATTTAATGATGCTAAAAGAAGTGAGAA
AAAAGTTGCTGTTATAGATTATGGTGTTAAAACAAATATCTTAAATGAACTTGTAGAAGTAGGTTTTGAAGTAGAAGTTT
ATCCATATAATGTTAAAGCAGATGAGTTGATTACTCTGTATAAAAAAGGTGAAATTCAAGGCGTGTTTCTTTCTAATGGT
CCAGGTGAGCCAAGGATTTTAAAACAAGAGATTGCAGAGATTAAAAAACTTGCAGAAGCTAAAATTCCTATGCTTGGAAT
TTGCTTAGGTCACCAGCTTTTAAGTAATGCTTTTGGATATGAAACTTATAAAATGAAATTTGGACAACATGGAGCAAATC
ATCCTGTGATCAATCTTGATACAAAAACGGTAGAAATTACCGCACAAAATCATAATTATAATGTTCCAGAAGAATTAGCT
CAAGTGGCTCATATCACTCATAGAAATTTATTTGGCGATAATGTTGAAGGAGTAAGATATAAAGACTATCCTATTATTTC
AGTACAACATCATCCAGAAAGTTCTTCAGGTCCTCATGAAAGTAAGTATATTTTTAAAGAATTTATGAATTTGATGTGA

Upstream 100 bases:

>100_bases
TTAGCAATTATAAAACAAAACCTATTCCAGGTAGCGAAGAATACGATCTTATCTTTGAAAAACTCTATCAAGAAGAGCTC
AGAAAAAAAGGTATGTTTTA

Downstream 100 bases:

>100_bases
GTATAGATTTTTTAGCTATTATCAGCGTTGCTTTTTTATCTAGTTTTGGACATTGCTATTCTATGTGTGGTGGTTTTAAT
TTACTTTTTATAAATTTGAA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKAYIYLENDIFLSAKAFGKSGTFFGELVFNTSLTGYQEIISDPSYAGQFIVFSMPEIGIVGTNENDNESKEIFASGVLM
RELSSSFSNFRAKESLQDYLEKHGKIGIYELDTRYLVKMIRNNGNLRAVISTEISNKEDLKIALEKSAKIDEVNFVKEVS
TKKNYSHKQGVWNASFQKFNDAKRSEKKVAVIDYGVKTNILNELVEVGFEVEVYPYNVKADELITLYKKGEIQGVFLSNG
PGEPRILKQEIAEIKKLAEAKIPMLGICLGHQLLSNAFGYETYKMKFGQHGANHPVINLDTKTVEITAQNHNYNVPEELA
QVAHITHRNLFGDNVEGVRYKDYPIISVQHHPESSSGPHESKYIFKEFMNLM

Sequences:

>Translated_372_residues
MKAYIYLENDIFLSAKAFGKSGTFFGELVFNTSLTGYQEIISDPSYAGQFIVFSMPEIGIVGTNENDNESKEIFASGVLM
RELSSSFSNFRAKESLQDYLEKHGKIGIYELDTRYLVKMIRNNGNLRAVISTEISNKEDLKIALEKSAKIDEVNFVKEVS
TKKNYSHKQGVWNASFQKFNDAKRSEKKVAVIDYGVKTNILNELVEVGFEVEVYPYNVKADELITLYKKGEIQGVFLSNG
PGEPRILKQEIAEIKKLAEAKIPMLGICLGHQLLSNAFGYETYKMKFGQHGANHPVINLDTKTVEITAQNHNYNVPEELA
QVAHITHRNLFGDNVEGVRYKDYPIISVQHHPESSSGPHESKYIFKEFMNLM
>Mature_372_residues
MKAYIYLENDIFLSAKAFGKSGTFFGELVFNTSLTGYQEIISDPSYAGQFIVFSMPEIGIVGTNENDNESKEIFASGVLM
RELSSSFSNFRAKESLQDYLEKHGKIGIYELDTRYLVKMIRNNGNLRAVISTEISNKEDLKIALEKSAKIDEVNFVKEVS
TKKNYSHKQGVWNASFQKFNDAKRSEKKVAVIDYGVKTNILNELVEVGFEVEVYPYNVKADELITLYKKGEIQGVFLSNG
PGEPRILKQEIAEIKKLAEAKIPMLGICLGHQLLSNAFGYETYKMKFGQHGANHPVINLDTKTVEITAQNHNYNVPEELA
QVAHITHRNLFGDNVEGVRYKDYPIISVQHHPESSSGPHESKYIFKEFMNLM

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI169790915, Length=382, Percent_Identity=31.413612565445, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI21361331, Length=382, Percent_Identity=31.413612565445, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=31.5926892950392, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=40.053050397878, Blast_Score=292, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI193204318, Length=384, Percent_Identity=30.46875, Blast_Score=191, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6324878, Length=385, Percent_Identity=33.2467532467532, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322331, Length=388, Percent_Identity=31.4432989690722, Blast_Score=185, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24642586, Length=390, Percent_Identity=31.5384615384615, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45555749, Length=390, Percent_Identity=31.5384615384615, Blast_Score=190, Evalue=1e-48,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_CAMJE (Q9PMG8)

Other databases:

- EMBL:   AL111168
- PIR:   C81296
- RefSeq:   YP_002344874.1
- ProteinModelPortal:   Q9PMG8
- SMR:   Q9PMG8
- IntAct:   Q9PMG8
- GeneID:   905785
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj1494c
- HOGENOM:   HBG286341
- OMA:   EADIPFF
- ProtClustDB:   PRK12564
- BioCyc:   CJEJ192222:CJ1494C-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 42095; Mature: 42095

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 268-268 ACT_SITE 351-351 ACT_SITE 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYIYLENDIFLSAKAFGKSGTFFGELVFNTSLTGYQEIISDPSYAGQFIVFSMPEIGI
CEEEEEEECCEEEEEECCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCEEEEEECCCEEE
VGTNENDNESKEIFASGVLMRELSSSFSNFRAKESLQDYLEKHGKIGIYELDTRYLVKMI
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHH
RNNGNLRAVISTEISNKEDLKIALEKSAKIDEVNFVKEVSTKKNYSHKQGVWNASFQKFN
HCCCCEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHCCCCCCHHHHHH
DAKRSEKKVAVIDYGVKTNILNELVEVGFEVEVYPYNVKADELITLYKKGEIQGVFLSNG
HHHHCCCEEEEEECCCHHHHHHHHHHCCCEEEEEEECCCHHHEEEEEECCCEEEEEEECC
PGEPRILKQEIAEIKKLAEAKIPMLGICLGHQLLSNAFGYETYKMKFGQHGANHPVINLD
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEC
TKTVEITAQNHNYNVPEELAQVAHITHRNLFGDNVEGVRYKDYPIISVQHHPESSSGPHE
CEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCCCCCCH
SKYIFKEFMNLM
HHHHHHHHHHCC
>Mature Secondary Structure
MKAYIYLENDIFLSAKAFGKSGTFFGELVFNTSLTGYQEIISDPSYAGQFIVFSMPEIGI
CEEEEEEECCEEEEEECCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCEEEEEECCCEEE
VGTNENDNESKEIFASGVLMRELSSSFSNFRAKESLQDYLEKHGKIGIYELDTRYLVKMI
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHH
RNNGNLRAVISTEISNKEDLKIALEKSAKIDEVNFVKEVSTKKNYSHKQGVWNASFQKFN
HCCCCEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHCCCCCCHHHHHH
DAKRSEKKVAVIDYGVKTNILNELVEVGFEVEVYPYNVKADELITLYKKGEIQGVFLSNG
HHHHCCCEEEEEECCCHHHHHHHHHHCCCEEEEEEECCCHHHEEEEEECCCEEEEEEECC
PGEPRILKQEIAEIKKLAEAKIPMLGICLGHQLLSNAFGYETYKMKFGQHGANHPVINLD
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEC
TKTVEITAQNHNYNVPEELAQVAHITHRNLFGDNVEGVRYKDYPIISVQHHPESSSGPHE
CEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCCCCCCH
SKYIFKEFMNLM
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204