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The map label for this gene is cofH [H]

Identifier: 57238412

GI number: 57238412

Start: 1460376

End: 1461440

Strand: Direct

Name: cofH [H]

Synonym: CJE1560

Alternate gene names: 57238412

Gene position: 1460376-1461440 (Clockwise)

Preceding gene: 57238407

Following gene: 57238413

Centisome position: 82.14

GC content: 33.33

Gene sequence:

>1065_bases
ATGAAAAATTTAATCACCAAACTAGAAAATGAAGAAAGATTAAACCAAGAAGAAGCCAATGCACTTTGGGATCTAGATCT
TTTTACCTTAGGCAAATACGCTCACAAAAAACGCACTAAACTTCATGGAAAAAAAGTCTATTTTAATATCAATCGCCATA
TAAATCCTACAAATATTTGTGCGGATACCTGTAAATTTTGCGCCTTTTCAGCGCACCGAAAAAATCCTAATCCTTATTTG
ATGACTCATGAAGAAATCATGAAAATCGTCGATGAAACTGTAAAACGAGAAACCAAAGAAGTGCATATCGTTTCAGCTCA
CAACAAAGATACAACTTGGCAATGGTATTTAGAAATTTTTAAAATGATAAAAGAAAAATATCCTAATCTTCATGTAAAAG
CTATGACTGCTGCTGAAATTGATTTTTTACATCGTCGTTTTAAAATGAGTTATGAAGAAGTGATAGAAAAAATGCTTGAA
TACGGCGTTGATTCCATGCCAGGCGGTGGAGCTGAAATTTTTGATGAAGAAGTGCGTAAGAAAATTTGTCATGGAAAAGT
TAGCAGTGAAAATTGGCTTAAAATTCATAAACTTTGGCATGAAAAAAGCAAGCAAAGTAATGCTACCATGCTTTTTGGAC
ATATAGAAGAAAGACATCACAGGATCGATCATATGTTAAGACTGCGTGATCTTCAAGATCAAACCGGTGGATTTAATGCT
TTTATCCCTCTAGTTTGGCAAAGGGATAATAGCTTTATACAAACTGATAAAATCATGGATAGTGAAGAAATTTTAAAAAC
CATTGCCATTTCTCGTTTAGTGCTTGATAATATCAAAAATATCAAAGCTTACTGGGCAACAATGACTTTAAATTTAGCCA
TGGTAGCACAAGAATTTGGAGCTAATGATTTAGACGGCACTATAGAAAAAGAAAGTATACAAAGTGCAGGTGGTGCAAAG
TCCGCCAAAGGCACAAGTTTAAAAACTTTTATCGATATGATAAAAACTTCAAATCTTATTCCTGTAGAACGCGATAGTTT
ATACAATGAGCTAAAAACCTACTAA

Upstream 100 bases:

>100_bases
TAACAAAAATATCTTTTATTTTGGCTTTTTCTATAAACTCTTTTTCAGTAAGCAAAAATTATAATGCTTTATTTAAAAAC
AGCAAAAGAGTAAAATTAAA

Downstream 100 bases:

>100_bases
GGAGAAAAAATGAGCTTTTTTAAACTCAAAGAAAACAATACGAGTTTTAAAACCGAAGTTATAGCAGGACTTACTACTTT
TTTAGCTATGGTTTACATTA

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKNLITKLENEERLNQEEANALWDLDLFTLGKYAHKKRTKLHGKKVYFNINRHINPTNICADTCKFCAFSAHRKNPNPYL
MTHEEIMKIVDETVKRETKEVHIVSAHNKDTTWQWYLEIFKMIKEKYPNLHVKAMTAAEIDFLHRRFKMSYEEVIEKMLE
YGVDSMPGGGAEIFDEEVRKKICHGKVSSENWLKIHKLWHEKSKQSNATMLFGHIEERHHRIDHMLRLRDLQDQTGGFNA
FIPLVWQRDNSFIQTDKIMDSEEILKTIAISRLVLDNIKNIKAYWATMTLNLAMVAQEFGANDLDGTIEKESIQSAGGAK
SAKGTSLKTFIDMIKTSNLIPVERDSLYNELKTY

Sequences:

>Translated_354_residues
MKNLITKLENEERLNQEEANALWDLDLFTLGKYAHKKRTKLHGKKVYFNINRHINPTNICADTCKFCAFSAHRKNPNPYL
MTHEEIMKIVDETVKRETKEVHIVSAHNKDTTWQWYLEIFKMIKEKYPNLHVKAMTAAEIDFLHRRFKMSYEEVIEKMLE
YGVDSMPGGGAEIFDEEVRKKICHGKVSSENWLKIHKLWHEKSKQSNATMLFGHIEERHHRIDHMLRLRDLQDQTGGFNA
FIPLVWQRDNSFIQTDKIMDSEEILKTIAISRLVLDNIKNIKAYWATMTLNLAMVAQEFGANDLDGTIEKESIQSAGGAK
SAKGTSLKTFIDMIKTSNLIPVERDSLYNELKTY
>Mature_354_residues
MKNLITKLENEERLNQEEANALWDLDLFTLGKYAHKKRTKLHGKKVYFNINRHINPTNICADTCKFCAFSAHRKNPNPYL
MTHEEIMKIVDETVKRETKEVHIVSAHNKDTTWQWYLEIFKMIKEKYPNLHVKAMTAAEIDFLHRRFKMSYEEVIEKMLE
YGVDSMPGGGAEIFDEEVRKKICHGKVSSENWLKIHKLWHEKSKQSNATMLFGHIEERHHRIDHMLRLRDLQDQTGGFNA
FIPLVWQRDNSFIQTDKIMDSEEILKTIAISRLVLDNIKNIKAYWATMTLNLAMVAQEFGANDLDGTIEKESIQSAGGAK
SAKGTSLKTFIDMIKTSNLIPVERDSLYNELKTY

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41264; Mature: 41264

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLITKLENEERLNQEEANALWDLDLFTLGKYAHKKRTKLHGKKVYFNINRHINPTNIC
CCHHHHHHCCHHHCCHHHHHHEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
ADTCKFCAFSAHRKNPNPYLMTHEEIMKIVDETVKRETKEVHIVSAHNKDTTWQWYLEIF
HHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCHHEEEEEEECCCCCHHHHHHHHH
KMIKEKYPNLHVKAMTAAEIDFLHRRFKMSYEEVIEKMLEYGVDSMPGGGAEIFDEEVRK
HHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KICHGKVSSENWLKIHKLWHEKSKQSNATMLFGHIEERHHRIDHMLRLRDLQDQTGGFNA
HHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FIPLVWQRDNSFIQTDKIMDSEEILKTIAISRLVLDNIKNIKAYWATMTLNLAMVAQEFG
EEEEEEECCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ANDLDGTIEKESIQSAGGAKSAKGTSLKTFIDMIKTSNLIPVERDSLYNELKTY
CCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKNLITKLENEERLNQEEANALWDLDLFTLGKYAHKKRTKLHGKKVYFNINRHINPTNIC
CCHHHHHHCCHHHCCHHHHHHEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
ADTCKFCAFSAHRKNPNPYLMTHEEIMKIVDETVKRETKEVHIVSAHNKDTTWQWYLEIF
HHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCHHEEEEEEECCCCCHHHHHHHHH
KMIKEKYPNLHVKAMTAAEIDFLHRRFKMSYEEVIEKMLEYGVDSMPGGGAEIFDEEVRK
HHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KICHGKVSSENWLKIHKLWHEKSKQSNATMLFGHIEERHHRIDHMLRLRDLQDQTGGFNA
HHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FIPLVWQRDNSFIQTDKIMDSEEILKTIAISRLVLDNIKNIKAYWATMTLNLAMVAQEFG
EEEEEEECCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ANDLDGTIEKESIQSAGGAKSAKGTSLKTFIDMIKTSNLIPVERDSLYNELKTY
CCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]