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The map label for this gene is nrfA [H]

Identifier: 57238399

GI number: 57238399

Start: 1441303

End: 1443135

Strand: Reverse

Name: nrfA [H]

Synonym: CJE1546

Alternate gene names: 57238399

Gene position: 1443135-1441303 (Counterclockwise)

Preceding gene: 57238400

Following gene: 57238398

Centisome position: 81.17

GC content: 34.15

Gene sequence:

>1833_bases
ATGAAAAAAAATATTTTACGCTTAGGTATTGTCGTTTTGGTTTTACTTATAGCGGGAGTTTTATGGCTAAATAATGATAT
CAATCAAAAAAAAGAAGATGAAGCAAATAAAAATGCCATTGCAGCAAATGCTGATTTTTCTTTGCTTAGTGATGATGATC
CAAATTTTGAAAAATGGGGTAAGGTTTTTCCAGAGCAACTAAAAATGTATTTAACGGTTGAAAAAGAAGAACCTAAGGCT
ACTGAATTTGGTGGTAATTTAGCTTATTCAAAATTAATTCGCTTTCCTCAATTAACCATACTTTGGGCAGGATATCCTTT
TAGTCTTGATTTCAATGAAGAAAGAGGGCATTTTTGGGTTCAAGTAGATCAAATGAAAACAGCAAGAAACAACAAAGATT
TTCTTAATGCTCACGGCCTTGCTGCTTTTAAAGGCCAGCCCGCAGCTTGTATGAATTGTCATAGTGGATGGACTCCATGG
CTTATAAAAAATGTTGCTAAAGGAGATTTTACTGCTTTTAACTCTACAAATTATTGGACTATGATTAAAAATATCCCAGC
CGTTGATGGTATAGTGGAAAATTCACCTGAACATGCAGGTCCACATGGTGGTAAAAGAATGGGTGTAACTTGTGCAGATT
GTCACAATCCAAATGATATGAGTTTAAGACTTACTCGTCCAGCAGCTATTAATGCTTTAGTTTCTAGAGGATATGAAAAA
GATCCAGTGCAAGGCGTAAAAGCTACAAGAGAAGAAATGAGAACTTTAGTTTGCTCTCAATGCCATGTTGAATACTATTT
TAAACCAACGGGGGAAAAAGTAAAAGTAATGGGTGAAACTATTGTAGATGATAGTTCTAAAAAATGGTGGAATGGAACTC
AGAAAAATTATGATGAGTATGAATTTTGGAGAGATGGTAATAAAGCTAAAGAGATTGAAACCGATGGTATAGTTTTAACT
TTTCCTTGGAGTGAGTGGAAAAAAGGGCAACCATTTAGAATTGAAATGTTAGATGATTATTATGATAAAGTTCGTGGAGT
ATTTGGAGCTGATTTTACTCATAAATTAACAGGGGCGCAAATTATTAAAATTCAACACCCAGAAAGCGAACTTTATAGTG
GCGGTGTGCATGCTGCAAATGGGGTAAGTTGCGTGGATTGTCATATGCCTTATGTTAGAGAAGGAGCTAAAAAGGTTACT
CAACACAATATCACCTCTCCTTTAAGAGATATTAACTCTGCTTGTAAGTCTTGTCATAAACAAAGCGAAGATTATTTAAA
AGCTCAAGTGCTTGACATACAAAATAGCGTTGCACATGATCAAAGAACTGCAGAGTATGCAATTGTTAGTTTGATTATGG
ATACTAAAAAACTACGCGATGAACTAGGTAATATGGAAAAATTCCAAAGCGATGGAAAAGCTGATGCGAAAAAAATTAGC
GAAGAACTAAAAGAAGTTTTAGAACTTCATCGTAAAGCTCAAATGAGAGCAGATTTTGTTAATGCTGAAAACTCAACTGG
TTTCCATAATCCTCGCGAAGCTTCAAGAATGTTATTGCAAGCTGTTGATATGGCAAGAATGGGACAAACTAAACTTGTAG
AAATCGCCGCAGCAAATGGAATTAAAGATTTTAAAACTTCAAATTTAGGTTTTGAAGATATTCAAAAATTTAATCCAGGA
GAGCTTTATTATAAAGTAGATGTTAATAATCATAAAGCTGGAGAGCGTTACTATGCAGATGAAAAAGATGTTAATGGTAA
TCCTCCAAAAGAACTTTTAGAGCATGATAAAGAGCTCGCTCCTTATAATTATCAAGTGATTGATAAAAAATAA

Upstream 100 bases:

>100_bases
TCAATGCTACAACAAATCCACATGCAGACAAGTCATTAAATTGTGCTTCTTGTCATAAAGATGTTGGACATAAACACGGA
ATTTAATAAGGAGAAGATGA

Downstream 100 bases:

>100_bases
TTCTTAAGCCCTATAAATTATGGGGCTTTTTCTTTTTTATTTTAAAAAATATAAACTTTTTATTTAAAATTCACTTTTGC
TTCACTTTGTTTTGTCAAAA

Product: cytochrome C552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MKKNILRLGIVVLVLLIAGVLWLNNDINQKKEDEANKNAIAANADFSLLSDDDPNFEKWGKVFPEQLKMYLTVEKEEPKA
TEFGGNLAYSKLIRFPQLTILWAGYPFSLDFNEERGHFWVQVDQMKTARNNKDFLNAHGLAAFKGQPAACMNCHSGWTPW
LIKNVAKGDFTAFNSTNYWTMIKNIPAVDGIVENSPEHAGPHGGKRMGVTCADCHNPNDMSLRLTRPAAINALVSRGYEK
DPVQGVKATREEMRTLVCSQCHVEYYFKPTGEKVKVMGETIVDDSSKKWWNGTQKNYDEYEFWRDGNKAKEIETDGIVLT
FPWSEWKKGQPFRIEMLDDYYDKVRGVFGADFTHKLTGAQIIKIQHPESELYSGGVHAANGVSCVDCHMPYVREGAKKVT
QHNITSPLRDINSACKSCHKQSEDYLKAQVLDIQNSVAHDQRTAEYAIVSLIMDTKKLRDELGNMEKFQSDGKADAKKIS
EELKEVLELHRKAQMRADFVNAENSTGFHNPREASRMLLQAVDMARMGQTKLVEIAAANGIKDFKTSNLGFEDIQKFNPG
ELYYKVDVNNHKAGERYYADEKDVNGNPPKELLEHDKELAPYNYQVIDKK

Sequences:

>Translated_610_residues
MKKNILRLGIVVLVLLIAGVLWLNNDINQKKEDEANKNAIAANADFSLLSDDDPNFEKWGKVFPEQLKMYLTVEKEEPKA
TEFGGNLAYSKLIRFPQLTILWAGYPFSLDFNEERGHFWVQVDQMKTARNNKDFLNAHGLAAFKGQPAACMNCHSGWTPW
LIKNVAKGDFTAFNSTNYWTMIKNIPAVDGIVENSPEHAGPHGGKRMGVTCADCHNPNDMSLRLTRPAAINALVSRGYEK
DPVQGVKATREEMRTLVCSQCHVEYYFKPTGEKVKVMGETIVDDSSKKWWNGTQKNYDEYEFWRDGNKAKEIETDGIVLT
FPWSEWKKGQPFRIEMLDDYYDKVRGVFGADFTHKLTGAQIIKIQHPESELYSGGVHAANGVSCVDCHMPYVREGAKKVT
QHNITSPLRDINSACKSCHKQSEDYLKAQVLDIQNSVAHDQRTAEYAIVSLIMDTKKLRDELGNMEKFQSDGKADAKKIS
EELKEVLELHRKAQMRADFVNAENSTGFHNPREASRMLLQAVDMARMGQTKLVEIAAANGIKDFKTSNLGFEDIQKFNPG
ELYYKVDVNNHKAGERYYADEKDVNGNPPKELLEHDKELAPYNYQVIDKK
>Mature_610_residues
MKKNILRLGIVVLVLLIAGVLWLNNDINQKKEDEANKNAIAANADFSLLSDDDPNFEKWGKVFPEQLKMYLTVEKEEPKA
TEFGGNLAYSKLIRFPQLTILWAGYPFSLDFNEERGHFWVQVDQMKTARNNKDFLNAHGLAAFKGQPAACMNCHSGWTPW
LIKNVAKGDFTAFNSTNYWTMIKNIPAVDGIVENSPEHAGPHGGKRMGVTCADCHNPNDMSLRLTRPAAINALVSRGYEK
DPVQGVKATREEMRTLVCSQCHVEYYFKPTGEKVKVMGETIVDDSSKKWWNGTQKNYDEYEFWRDGNKAKEIETDGIVLT
FPWSEWKKGQPFRIEMLDDYYDKVRGVFGADFTHKLTGAQIIKIQHPESELYSGGVHAANGVSCVDCHMPYVREGAKKVT
QHNITSPLRDINSACKSCHKQSEDYLKAQVLDIQNSVAHDQRTAEYAIVSLIMDTKKLRDELGNMEKFQSDGKADAKKIS
EELKEVLELHRKAQMRADFVNAENSTGFHNPREASRMLLQAVDMARMGQTKLVEIAAANGIKDFKTSNLGFEDIQKFNPG
ELYYKVDVNNHKAGERYYADEKDVNGNPPKELLEHDKELAPYNYQVIDKK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=455, Percent_Identity=29.8901098901099, Blast_Score=168, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 69197; Mature: 69197

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNILRLGIVVLVLLIAGVLWLNNDINQKKEDEANKNAIAANADFSLLSDDDPNFEKWG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCEEECCCCCCHHHHH
KVFPEQLKMYLTVEKEEPKATEFGGNLAYSKLIRFPQLTILWAGYPFSLDFNEERGHFWV
HHCHHHEEEEEEEECCCCCHHHCCCCHHHHHHHCCCCEEEEEECCCEEECCCCCCCEEEE
QVDQMKTARNNKDFLNAHGLAAFKGQPAACMNCHSGWTPWLIKNVAKGDFTAFNSTNYWT
EEHHHHHHCCCCCHHCCCCEEEECCCCCHHHCCCCCCCHHHHHHHCCCCCEEECCCCEEE
MIKNIPAVDGIVENSPEHAGPHGGKRMGVTCADCHNPNDMSLRLTRPAAINALVSRGYEK
EECCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECHHHHHHHHHCCCCC
DPVQGVKATREEMRTLVCSQCHVEYYFKPTGEKVKVMGETIVDDSSKKWWNGTQKNYDEY
CCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECHHHHCCCCCCCCCCCCCCCCHH
EFWRDGNKAKEIETDGIVLTFPWSEWKKGQPFRIEMLDDYYDKVRGVFGADFTHKLTGAQ
HHHCCCCCCCEECCCCEEEEECHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCCEECCCEE
IIKIQHPESELYSGGVHAANGVSCVDCHMPYVREGAKKVTQHNITSPLRDINSACKSCHK
EEEEECCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QSEDYLKAQVLDIQNSVAHDQRTAEYAIVSLIMDTKKLRDELGNMEKFQSDGKADAKKIS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHH
EELKEVLELHRKAQMRADFVNAENSTGFHNPREASRMLLQAVDMARMGQTKLVEIAAANG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
IKDFKTSNLGFEDIQKFNPGELYYKVDVNNHKAGERYYADEKDVNGNPPKELLEHDKELA
CCHHCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHCC
PYNYQVIDKK
CCCEEEECCC
>Mature Secondary Structure
MKKNILRLGIVVLVLLIAGVLWLNNDINQKKEDEANKNAIAANADFSLLSDDDPNFEKWG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCEEECCCCCCHHHHH
KVFPEQLKMYLTVEKEEPKATEFGGNLAYSKLIRFPQLTILWAGYPFSLDFNEERGHFWV
HHCHHHEEEEEEEECCCCCHHHCCCCHHHHHHHCCCCEEEEEECCCEEECCCCCCCEEEE
QVDQMKTARNNKDFLNAHGLAAFKGQPAACMNCHSGWTPWLIKNVAKGDFTAFNSTNYWT
EEHHHHHHCCCCCHHCCCCEEEECCCCCHHHCCCCCCCHHHHHHHCCCCCEEECCCCEEE
MIKNIPAVDGIVENSPEHAGPHGGKRMGVTCADCHNPNDMSLRLTRPAAINALVSRGYEK
EECCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECHHHHHHHHHCCCCC
DPVQGVKATREEMRTLVCSQCHVEYYFKPTGEKVKVMGETIVDDSSKKWWNGTQKNYDEY
CCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECHHHHCCCCCCCCCCCCCCCCHH
EFWRDGNKAKEIETDGIVLTFPWSEWKKGQPFRIEMLDDYYDKVRGVFGADFTHKLTGAQ
HHHCCCCCCCEECCCCEEEEECHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCCEECCCEE
IIKIQHPESELYSGGVHAANGVSCVDCHMPYVREGAKKVTQHNITSPLRDINSACKSCHK
EEEEECCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QSEDYLKAQVLDIQNSVAHDQRTAEYAIVSLIMDTKKLRDELGNMEKFQSDGKADAKKIS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHH
EELKEVLELHRKAQMRADFVNAENSTGFHNPREASRMLLQAVDMARMGQTKLVEIAAANG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
IKDFKTSNLGFEDIQKFNPGELYYKVDVNNHKAGERYYADEKDVNGNPPKELLEHDKELA
CCHHCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHCC
PYNYQVIDKK
CCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA