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The map label for this gene is def

Identifier: 57237196

GI number: 57237196

Start: 185000

End: 185527

Strand: Reverse

Name: def

Synonym: CJE0184

Alternate gene names: 57237196

Gene position: 185527-185000 (Counterclockwise)

Preceding gene: 57237197

Following gene: 57237195

Centisome position: 10.44

GC content: 29.55

Gene sequence:

>528_bases
ATGGTTAGAAAAATTATCACCTATCCCAATCCTAGACTTTTTTTAAACTCTGAAATTGTAAATAAATTTGATACAGAACT
TCACACCTTGCTTGATGATATGTATGAAACTATGATAGCAAGTAATGGAGTAGGTTTAGCAGCTATTCAAGTGGATATTC
CTTTGCGTGTTTTACTTGTAAATATTTTTGATGAAAACGATGAACAAAAAAAAGAAGATTTGCTAGAGATTATCAATCCT
GAAATTATTCCTTTAGATGAAGAAATGATCACTTGCACAGAAGGGTGTTTGAGTGTGCCAGATTTTTTTGAAGAAGTGAA
ACGCTATAATCATATCTTGCTTAAATATCAAGATCGCTTTGGAGAATTTAAAGAACTTGAAGCAAAAGGTTTTTTAGCTG
TGGCTATTCAACACGAAAATGATCATTTAAATGGACATTTATTTATAGAAAAAATTTCCTTTGCTAAGCGTCAAAAATTC
GATAAAGAATTTAAGAAAAAGAAGAAAAATCATAAAAAAGAGAAATGA

Upstream 100 bases:

>100_bases
TGTCAGCACAAGAAGCTAAAGAATATGGGCTGATTGATAAGGTTTTGGAAAAAAGTTTTAAATGATTTCTTTGGAGATCA
TTTAAAACAAAGGTTTTTTA

Downstream 100 bases:

>100_bases
AAAAACTAAAGTGCATAAGCTTTCATGAAGGCTTAGACATTATAGAGGTAGAATCCACTTTTACAAGAGGTTTGCCAAAT
TTAAGCATAGTAGGGCTTGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MVRKIITYPNPRLFLNSEIVNKFDTELHTLLDDMYETMIASNGVGLAAIQVDIPLRVLLVNIFDENDEQKKEDLLEIINP
EIIPLDEEMITCTEGCLSVPDFFEEVKRYNHILLKYQDRFGEFKELEAKGFLAVAIQHENDHLNGHLFIEKISFAKRQKF
DKEFKKKKKNHKKEK

Sequences:

>Translated_175_residues
MVRKIITYPNPRLFLNSEIVNKFDTELHTLLDDMYETMIASNGVGLAAIQVDIPLRVLLVNIFDENDEQKKEDLLEIINP
EIIPLDEEMITCTEGCLSVPDFFEEVKRYNHILLKYQDRFGEFKELEAKGFLAVAIQHENDHLNGHLFIEKISFAKRQKF
DKEFKKKKKNHKKEK
>Mature_175_residues
MVRKIITYPNPRLFLNSEIVNKFDTELHTLLDDMYETMIASNGVGLAAIQVDIPLRVLLVNIFDENDEQKKEDLLEIINP
EIIPLDEEMITCTEGCLSVPDFFEEVKRYNHILLKYQDRFGEFKELEAKGFLAVAIQHENDHLNGHLFIEKISFAKRQKF
DKEFKKKKKNHKKEK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=140, Percent_Identity=32.1428571428571, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=38.1818181818182, Blast_Score=112, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24645728, Length=141, Percent_Identity=30.4964539007092, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CAMJE (Q9PIT8)

Other databases:

- EMBL:   AL111168
- PIR:   F81437
- RefSeq:   YP_002343649.1
- ProteinModelPortal:   Q9PIT8
- SMR:   Q9PIT8
- IntAct:   Q9PIT8
- GeneID:   904534
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj0191c
- HOGENOM:   HBG665227
- OMA:   AADMAET
- ProtClustDB:   PRK00150
- BioCyc:   CJEJ192222:CJ0191C-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20581; Mature: 20581

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKIITYPNPRLFLNSEIVNKFDTELHTLLDDMYETMIASNGVGLAAIQVDIPLRVLLV
CCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHEEE
NIFDENDEQKKEDLLEIINPEIIPLDEEMITCTEGCLSVPDFFEEVKRYNHILLKYQDRF
HHHCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEHHHC
GEFKELEAKGFLAVAIQHENDHLNGHLFIEKISFAKRQKFDKEFKKKKKNHKKEK
CCHHHHCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVRKIITYPNPRLFLNSEIVNKFDTELHTLLDDMYETMIASNGVGLAAIQVDIPLRVLLV
CCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHEEE
NIFDENDEQKKEDLLEIINPEIIPLDEEMITCTEGCLSVPDFFEEVKRYNHILLKYQDRF
HHHCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEHHHC
GEFKELEAKGFLAVAIQHENDHLNGHLFIEKISFAKRQKFDKEFKKKKKNHKKEK
CCHHHHCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204