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The map label for this gene is yifB [H]

Identifier: 57237195

GI number: 57237195

Start: 183498

End: 185003

Strand: Reverse

Name: yifB [H]

Synonym: CJE0183

Alternate gene names: 57237195

Gene position: 185003-183498 (Counterclockwise)

Preceding gene: 57237196

Following gene: 57237194

Centisome position: 10.41

GC content: 30.35

Gene sequence:

>1506_bases
ATGAAAAAACTAAAGTGCATAAGCTTTCATGAAGGCTTAGACATTATAGAGGTAGAATCCACTTTTACAAGAGGTTTGCC
AAATTTAAGCATAGTAGGGCTTGCAAGTACAGCGATTAAGGAAAGTGTCGAACGCATCAAAGCCACACTTTTAAGTTGTG
ATTTTGCTTTTCCTGCTAAAAAAATCACTATCAATCTTAGTCCTTCAGGTATCCCAAAAAAGGGATCACATTTTGATTTG
GCTATCGCTTTATTAATCCTTTTGCAAAATGAAGAATTAGATGACTTTTTTGTAGTAGGAGAATTAGGACTTGATGGAAG
TATTAAAAGCACAAATGAGCTTTTTTCTTTGCTTTTGTTTCTCTCTGCTAAAATTGAAAAAGCTAAAATCGTTGTTCCAA
AAAGTATAGCACAAAAAGCTTCCATGATACCAAATTTAGAGGTTTATGGACTTGAAAATTTAAATGAAGCAATAGAGTTT
TTTAAAGAAAAAAATTATGAAAATTTTCGTTTTTCTCACAATCATCCTTTATTCGCAAGTCCTTTACAAATAGAAAATGA
AATTTTTTTACAAAATATGGATTTTAAACTTGATTTTAAAGATATCAAAGGGCAGGAAAAAGCTAAAAGAGCTTGTATGA
TAGCTGCTTTAGGTATGCATAATATCTTATTTGAAGGAAGTCCAGGAAGTGGTAAAAGTATGTGTGCTAAACGCCTTGTT
TATATCATGCCTCCTCAAAGTTTAAGTGAAATTTTAATGCAAAATGCTTATATGTCTTTAGATTCAAAAGATTGTGAATT
TACTAAAATTCGTGCTTTTCGTCATCCTCATCATACTTCTACAAGAGCAAGTATTTTTGGCGGAGGAACAAAAAATGCTA
GAATTGGAGAAGTCGCACTTGCAAATGGTGGAGTATTATTTTTTGATGAATTTCCACATTTTAATAAGCAAATCATAGAG
AGTTTAAGAGAGCCTTTAGAGGATCATAAAATTCATATTTCAAGAGTAAATTCTAAAATCACTTATGAAACAAAATTTAG
CTTTATAGCTGCCCAAAATCCTTGTCCTTGTGGAAATTTATTTTCTAAAAATCTTTCTTGTGTTTGTAGTGAAAATGAGA
TTAAAAAATACAAAAATCACATTTCAGCTCCTATTATGGATAGGATTGATCTTTATGTAGCTATGGATGAGATCAGCAAG
GATGATAAAACGAGTATAAGTTCTAAAGAAATGAGTGAAAAAATTTTACAAGCTTTTATTTTTAGTAAAAAACGGGGGCA
AAAAGAATTTAATGGGAAATTAAAAGATGAAGATTTAAGTCGTTTTTGTGTATTCGAAAAAGATGCCAAAGATACGCTAG
ATTTAGCAATTTCACGTTATAATCTTTCTTTGCGATCGCTTAATAAAATTTTAAAAGTTTCAAGAAGTATAGCGGATTTA
GAACAAAGTCTTAATATTAACAAAACCCATATTTTAGAAGCTTTAAGCTTTAGAGCAAGGAATTAA

Upstream 100 bases:

>100_bases
ATTTAAATGGACATTTATTTATAGAAAAAATTTCCTTTGCTAAGCGTCAAAAATTCGATAAAGAATTTAAGAAAAAGAAG
AAAAATCATAAAAAAGAGAA

Downstream 100 bases:

>100_bases
AATGGAAAATAAAAGTATAGCTATCTTTATAGATGCAGAAAATATCCCTGCAAAATACACTAAAAGTATTTTTGATATTG
CTTCAGATTACGGAGAAGTT

Product: Mg chelatase-related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKKLKCISFHEGLDIIEVESTFTRGLPNLSIVGLASTAIKESVERIKATLLSCDFAFPAKKITINLSPSGIPKKGSHFDL
AIALLILLQNEELDDFFVVGELGLDGSIKSTNELFSLLLFLSAKIEKAKIVVPKSIAQKASMIPNLEVYGLENLNEAIEF
FKEKNYENFRFSHNHPLFASPLQIENEIFLQNMDFKLDFKDIKGQEKAKRACMIAALGMHNILFEGSPGSGKSMCAKRLV
YIMPPQSLSEILMQNAYMSLDSKDCEFTKIRAFRHPHHTSTRASIFGGGTKNARIGEVALANGGVLFFDEFPHFNKQIIE
SLREPLEDHKIHISRVNSKITYETKFSFIAAQNPCPCGNLFSKNLSCVCSENEIKKYKNHISAPIMDRIDLYVAMDEISK
DDKTSISSKEMSEKILQAFIFSKKRGQKEFNGKLKDEDLSRFCVFEKDAKDTLDLAISRYNLSLRSLNKILKVSRSIADL
EQSLNINKTHILEALSFRARN

Sequences:

>Translated_501_residues
MKKLKCISFHEGLDIIEVESTFTRGLPNLSIVGLASTAIKESVERIKATLLSCDFAFPAKKITINLSPSGIPKKGSHFDL
AIALLILLQNEELDDFFVVGELGLDGSIKSTNELFSLLLFLSAKIEKAKIVVPKSIAQKASMIPNLEVYGLENLNEAIEF
FKEKNYENFRFSHNHPLFASPLQIENEIFLQNMDFKLDFKDIKGQEKAKRACMIAALGMHNILFEGSPGSGKSMCAKRLV
YIMPPQSLSEILMQNAYMSLDSKDCEFTKIRAFRHPHHTSTRASIFGGGTKNARIGEVALANGGVLFFDEFPHFNKQIIE
SLREPLEDHKIHISRVNSKITYETKFSFIAAQNPCPCGNLFSKNLSCVCSENEIKKYKNHISAPIMDRIDLYVAMDEISK
DDKTSISSKEMSEKILQAFIFSKKRGQKEFNGKLKDEDLSRFCVFEKDAKDTLDLAISRYNLSLRSLNKILKVSRSIADL
EQSLNINKTHILEALSFRARN
>Mature_501_residues
MKKLKCISFHEGLDIIEVESTFTRGLPNLSIVGLASTAIKESVERIKATLLSCDFAFPAKKITINLSPSGIPKKGSHFDL
AIALLILLQNEELDDFFVVGELGLDGSIKSTNELFSLLLFLSAKIEKAKIVVPKSIAQKASMIPNLEVYGLENLNEAIEF
FKEKNYENFRFSHNHPLFASPLQIENEIFLQNMDFKLDFKDIKGQEKAKRACMIAALGMHNILFEGSPGSGKSMCAKRLV
YIMPPQSLSEILMQNAYMSLDSKDCEFTKIRAFRHPHHTSTRASIFGGGTKNARIGEVALANGGVLFFDEFPHFNKQIIE
SLREPLEDHKIHISRVNSKITYETKFSFIAAQNPCPCGNLFSKNLSCVCSENEIKKYKNHISAPIMDRIDLYVAMDEISK
DDKTSISSKEMSEKILQAFIFSKKRGQKEFNGKLKDEDLSRFCVFEKDAKDTLDLAISRYNLSLRSLNKILKVSRSIADL
EQSLNINKTHILEALSFRARN

Specific function: Unknown

COG id: COG0606

COG function: function code O; Predicted ATPase with chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Mg-chelatase subunits D/I family. ComM subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693202, Length=496, Percent_Identity=33.2661290322581, Blast_Score=277, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004482
- InterPro:   IPR000523
- InterPro:   IPR020568
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01078 Mg_chelatase [H]

EC number: NA

Molecular weight: Translated: 56458; Mature: 56458

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKCISFHEGLDIIEVESTFTRGLPNLSIVGLASTAIKESVERIKATLLSCDFAFPAK
CCCCEEEEHHCCCCEEEECCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCE
KITINLSPSGIPKKGSHFDLAIALLILLQNEELDDFFVVGELGLDGSIKSTNELFSLLLF
EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
LSAKIEKAKIVVPKSIAQKASMIPNLEVYGLENLNEAIEFFKEKNYENFRFSHNHPLFAS
HHCCCCCEEEECCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCEEC
PLQIENEIFLQNMDFKLDFKDIKGQEKAKRACMIAALGMHNILFEGSPGSGKSMCAKRLV
CCCCCCEEEEECCCCEEEHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCEE
YIMPPQSLSEILMQNAYMSLDSKDCEFTKIRAFRHPHHTSTRASIFGGGTKNARIGEVAL
EEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCEECEEEE
ANGGVLFFDEFPHFNKQIIESLREPLEDHKIHISRVNSKITYETKFSFIAAQNPCPCGNL
ECCCEEEEECCCCCCHHHHHHHHCCHHHCCEEEEECCCEEEEEEEEEEEEECCCCCCCHH
FSKNLSCVCSENEIKKYKNHISAPIMDRIDLYVAMDEISKDDKTSISSKEMSEKILQAFI
HHCCCEEEECHHHHHHHHHHCCCCHHHHHHHEEEHHHCCCCCCCCCCHHHHHHHHHHHHH
FSKKRGQKEFNGKLKDEDLSRFCVFEKDAKDTLDLAISRYNLSLRSLNKILKVSRSIADL
HHHHCCCHHHCCCCCCCCHHHEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EQSLNINKTHILEALSFRARN
HHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLKCISFHEGLDIIEVESTFTRGLPNLSIVGLASTAIKESVERIKATLLSCDFAFPAK
CCCCEEEEHHCCCCEEEECCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCE
KITINLSPSGIPKKGSHFDLAIALLILLQNEELDDFFVVGELGLDGSIKSTNELFSLLLF
EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
LSAKIEKAKIVVPKSIAQKASMIPNLEVYGLENLNEAIEFFKEKNYENFRFSHNHPLFAS
HHCCCCCEEEECCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCEEC
PLQIENEIFLQNMDFKLDFKDIKGQEKAKRACMIAALGMHNILFEGSPGSGKSMCAKRLV
CCCCCCEEEEECCCCEEEHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCEE
YIMPPQSLSEILMQNAYMSLDSKDCEFTKIRAFRHPHHTSTRASIFGGGTKNARIGEVAL
EEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCEECEEEE
ANGGVLFFDEFPHFNKQIIESLREPLEDHKIHISRVNSKITYETKFSFIAAQNPCPCGNL
ECCCEEEEECCCCCCHHHHHHHHCCHHHCCEEEEECCCEEEEEEEEEEEEECCCCCCCHH
FSKNLSCVCSENEIKKYKNHISAPIMDRIDLYVAMDEISKDDKTSISSKEMSEKILQAFI
HHCCCEEEECHHHHHHHHHHCCCCHHHHHHHEEEHHHCCCCCCCCCCHHHHHHHHHHHHH
FSKKRGQKEFNGKLKDEDLSRFCVFEKDAKDTLDLAISRYNLSLRSLNKILKVSRSIADL
HHHHCCCHHHCCCCCCCCHHHEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EQSLNINKTHILEALSFRARN
HHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]