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The map label for this gene is ansA [H]

Identifier: 57236941

GI number: 57236941

Start: 35798

End: 36793

Strand: Direct

Name: ansA [H]

Synonym: CJE0029

Alternate gene names: 57236941

Gene position: 35798-36793 (Clockwise)

Preceding gene: 57236940

Following gene: 57237045

Centisome position: 2.01

GC content: 34.24

Gene sequence:

>996_bases
ATGAAAAAAGCTAAATCAAGGATTGCTATTTTAGGAACAGGCGGAACGATAGCAGGCTTCATTGATAGCACTATTGCTAC
AACAGGATATACGGCCGGAGCCATTGATATAGATGTATTAATTAAAGCAGTTCCGCAAATTAGAGATTTAGCAGATATTA
GTTGGGAGCAAATAGCAAATATTGATAGTTCAAATATGTGTGATGAAATTTGGTTAAGGCTTGCTAAAAAAATTGCTAAG
CTTTTTGCTGAGGGGATAGACGGTGTGGTGATAACTCATGGTACTGATACTATGGAAGAAACTGCTTATTTTTTAAATTT
AACCATTAAAAGTGATAAGCCCGTAGTTCTAGTTGGCGCTATGCGTCCATCAACTGCTATAAGTGCGGATGGTCCGAAAA
ATCTTTATAATGCAGTGGCATTAGTTGCCAATAAAGAAGCTAAGAATAAGGGTGTTATGGTAGCTATAAATGATAAAATT
TTAAGCGCTAGAGGCGTAGTAAAAACTCATAGTTTAAATGTTGATGCTTTCTCTTCTCCTGATTTTGGGGATTTGGGTTA
TATAGTAGATGGTAAAGTCTTTTTCTATAATAATGTTACTAAAGCTCACACAAAAAACGCTCCTTTTGATGTAAGCAAAC
TCACAAGTTTGCCAAAAGTAGATATTCTTTATAGCTATTCAAATGATGGTAGTGGTGTCGCTGCAAAAGCTTTATTTGAA
CATGGAACTAAGGGCATAGTTGTTGCAGGAAGTGGAGCAGGAAGTATACACAAAAATCAAAAAGATGTTTTAAAAGAGCT
TTTAAAAAAAGGGCTTAAGGTTGTTGTAAGTTCAAGGGTTGTAGCAGGTTGTGTTGCTGTGAGTGATTCAGATGAAAAAT
TAGGTTTTATTAGTGCTGAAGATTTAAATCCTCAAAAAGCAAGAGTTTTGCTTATATTAGCACTTACAAAAACAAGTGAT
CCTAAAAAAATTCAAGAGTATTTTTTAAAATATTAA

Upstream 100 bases:

>100_bases
TATAAAAATTTTTACTTATAATTTGCATTAATAACATTAAAAAATTAATATACAAGAGATTAAAAAATTATTTATATGTT
GTCTTTTTATAGGAGTATGC

Downstream 100 bases:

>100_bases
AATTTTTTAAATATTTCTAAACTTAGGGAACTTATCTTCCCTAAGTCAAGCCTTTCAATCCTATTAATAGTATATTATAT
CTTTATATTATATCTTGTAT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase 2; L-asparagine amidohydrolase 2 [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKAKSRIAILGTGGTIAGFIDSTIATTGYTAGAIDIDVLIKAVPQIRDLADISWEQIANIDSSNMCDEIWLRLAKKIAK
LFAEGIDGVVITHGTDTMEETAYFLNLTIKSDKPVVLVGAMRPSTAISADGPKNLYNAVALVANKEAKNKGVMVAINDKI
LSARGVVKTHSLNVDAFSSPDFGDLGYIVDGKVFFYNNVTKAHTKNAPFDVSKLTSLPKVDILYSYSNDGSGVAAKALFE
HGTKGIVVAGSGAGSIHKNQKDVLKELLKKGLKVVVSSRVVAGCVAVSDSDEKLGFISAEDLNPQKARVLLILALTKTSD
PKKIQEYFLKY

Sequences:

>Translated_331_residues
MKKAKSRIAILGTGGTIAGFIDSTIATTGYTAGAIDIDVLIKAVPQIRDLADISWEQIANIDSSNMCDEIWLRLAKKIAK
LFAEGIDGVVITHGTDTMEETAYFLNLTIKSDKPVVLVGAMRPSTAISADGPKNLYNAVALVANKEAKNKGVMVAINDKI
LSARGVVKTHSLNVDAFSSPDFGDLGYIVDGKVFFYNNVTKAHTKNAPFDVSKLTSLPKVDILYSYSNDGSGVAAKALFE
HGTKGIVVAGSGAGSIHKNQKDVLKELLKKGLKVVVSSRVVAGCVAVSDSDEKLGFISAEDLNPQKARVLLILALTKTSD
PKKIQEYFLKY
>Mature_331_residues
MKKAKSRIAILGTGGTIAGFIDSTIATTGYTAGAIDIDVLIKAVPQIRDLADISWEQIANIDSSNMCDEIWLRLAKKIAK
LFAEGIDGVVITHGTDTMEETAYFLNLTIKSDKPVVLVGAMRPSTAISADGPKNLYNAVALVANKEAKNKGVMVAINDKI
LSARGVVKTHSLNVDAFSSPDFGDLGYIVDGKVFFYNNVTKAHTKNAPFDVSKLTSLPKVDILYSYSNDGSGVAAKALFE
HGTKGIVVAGSGAGSIHKNQKDVLKELLKKGLKVVVSSRVVAGCVAVSDSDEKLGFISAEDLNPQKARVLLILALTKTSD
PKKIQEYFLKY

Specific function: Catalyzes the conversion of L-asparagine to L-aspartate and ammonium [H]

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=306, Percent_Identity=51.9607843137255, Blast_Score=309, Evalue=1e-85,
Organism=Escherichia coli, GI1788065, Length=337, Percent_Identity=25.5192878338279, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=351, Percent_Identity=25.6410256410256, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558280, Length=351, Percent_Identity=25.6410256410256, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=351, Percent_Identity=25.6410256410256, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193207374, Length=351, Percent_Identity=25.6410256410256, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=326, Percent_Identity=40.1840490797546, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6323187, Length=326, Percent_Identity=40.1840490797546, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6323186, Length=326, Percent_Identity=40.1840490797546, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6323184, Length=326, Percent_Identity=40.1840490797546, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6320527, Length=337, Percent_Identity=36.2017804154303, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24645530, Length=274, Percent_Identity=31.021897810219, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639609, Length=262, Percent_Identity=27.4809160305344, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35365; Mature: 35365

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAKSRIAILGTGGTIAGFIDSTIATTGYTAGAIDIDVLIKAVPQIRDLADISWEQIAN
CCCCCCEEEEEECCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHC
IDSSNMCDEIWLRLAKKIAKLFAEGIDGVVITHGTDTMEETAYFLNLTIKSDKPVVLVGA
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCEEEEEEEEECCCCEEEEEC
MRPSTAISADGPKNLYNAVALVANKEAKNKGVMVAINDKILSARGVVKTHSLNVDAFSSP
CCCCCEECCCCCHHHHHHHHHEECCCCCCCCEEEEECCCEECCCCEEEEEEECCCCCCCC
DFGDLGYIVDGKVFFYNNVTKAHTKNAPFDVSKLTSLPKVDILYSYSNDGSGVAAKALFE
CCCCCCEEEECEEEEEECCCHHCCCCCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
HGTKGIVVAGSGAGSIHKNQKDVLKELLKKGLKVVVSSRVVAGCVAVSDSDEKLGFISAE
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEECC
DLNPQKARVLLILALTKTSDPKKIQEYFLKY
CCCCCCEEEEEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKAKSRIAILGTGGTIAGFIDSTIATTGYTAGAIDIDVLIKAVPQIRDLADISWEQIAN
CCCCCCEEEEEECCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHC
IDSSNMCDEIWLRLAKKIAKLFAEGIDGVVITHGTDTMEETAYFLNLTIKSDKPVVLVGA
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCEEEEEEEEECCCCEEEEEC
MRPSTAISADGPKNLYNAVALVANKEAKNKGVMVAINDKILSARGVVKTHSLNVDAFSSP
CCCCCEECCCCCHHHHHHHHHEECCCCCCCCEEEEECCCEECCCCEEEEEEECCCCCCCC
DFGDLGYIVDGKVFFYNNVTKAHTKNAPFDVSKLTSLPKVDILYSYSNDGSGVAAKALFE
CCCCCCEEEECEEEEEECCCHHCCCCCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
HGTKGIVVAGSGAGSIHKNQKDVLKELLKKGLKVVVSSRVVAGCVAVSDSDEKLGFISAE
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEECC
DLNPQKARVLLILALTKTSDPKKIQEYFLKY
CCCCCCEEEEEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]