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The map label for this gene is purB-1 [H]

Identifier: 57236935

GI number: 57236935

Start: 26513

End: 27841

Strand: Direct

Name: purB-1 [H]

Synonym: CJE0023

Alternate gene names: 57236935

Gene position: 26513-27841 (Clockwise)

Preceding gene: 57236928

Following gene: 57236936

Centisome position: 1.49

GC content: 33.63

Gene sequence:

>1329_bases
ATGGTTGAAAGATATAGCAGAGAAATCATGGCTAAAAAATGGGACATGCAAGCAAAATACGATGCGTGGTTAAAAGTAGA
ATTAGCCGCTGTAAAAGCTTGGAATAAACTCGGTCTCATTAATGACACAGACTGCGAAAAAATTCTTAAAAATGCAAAAT
TTGATATTGCAAGAATAGACGAAATAGAAAAAACCACAAAACACGATGTTATCGCTTTTCTTACAAGTGTAAGTGAAAGT
TTGGGAGAAGAGAGTCGTTTTGTGCATTATGCAATGACAAGTTCTGATTGTATAGATACGGCTGTTGCTTTACAGATAAA
AGAGAGTTTGGAGCTTATTTTAGAAGATGTTTCTTTGCTTTTAGAAGTGATCAAAAAGCGTGCTTTGGAGCATAAAAATA
CCTTAATGGTAGGAAGAAGTCATGGAATTCATGGAGAGCCTATCACTTTTGGTTTAGTGTTAGCTATTTGGTATGATGAA
ATTTTGCATGCAAAAGAGCTTTTAGAACATGCAAAAGAAGTGATAAGCTATGGAAAAATCAGTGGTGCAATGGGAAATTT
TGCTCATGCTCCTTTAGAATTTGAAGAAGAAGTTTGTAAAAATTTAGGTCTTAAAGCTGCCCCTGTTTCAAATCAAGTGA
TCCAAAGAGATCGTTATGCTCAAGTTATTTCTGCTATTGCAATTTTAGCTTCAAGTTGTGAGCAAATCGCTGTAGCGATA
AGACATTTTCAAAGAACTGAAGTTTATGAGGCTGAAGAGTATTTTAGTGTAGGACAAAAAGGAAGTTCAGCTATGCCACA
TAAAAGAAATCCTGTTTTAAGTGAAAATATCACAGGGCTTTGTCGTGTGCTTAGATCTTTTGTAACTCCAGCTTTAGAAA
ATGTTGCACTTTGGCATGAAAGAGATATATCTCATTCTAGTGTTGAAAGATTTATTTTGCCTGATGCTTTTATCACGGCT
GATTTTATGCTTATGCGTCTTACAAATTTGATTGATAAGCTTTTAGTTTATCCTGAAAATATGATGAAAAATTTAAATCT
TACAGGCGGGCTTGTATTTTCAGGGAGGGTGCTTTTAGAGCTTCCATTTAAAGGCATTAGTCGTGAAGAAGCTTATAAAA
TAGTTCAAAGAAACGCTATGAAAGTTTGGGCAGATTTGCAAAATGGTAAGGCTGCTATCAATGAAAAAAATGAAAGTTTG
TTTTTGTTAGCGCTTTTAAGCGATGAAGATTTAAGAAAAAGTTTGAGCGAAGAGGATGTAAGAAAATGTTTCGATTATAA
CTACTATACAAAAAATGTTGATGTAATTTTTAAAAGAACATTTAAATAA

Upstream 100 bases:

>100_bases
AGTTTTATTTTTATGTAAGCCATTTAATAACTTTCTTAGGATAATTTGATATAATTTCTGTTTTTTAAAATATAAAATTA
TAGCTAAAAAGGTTTCAAAG

Downstream 100 bases:

>100_bases
AAAGCAAGGAAGGCAATGAAAGTTATTAAAAGAAATGGTCGTACAGAAGAATTGGATGTTTCTAAGATCAAAAAATGCAC
CTCTGATGCTGTAAAAGATT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MVERYSREIMAKKWDMQAKYDAWLKVELAAVKAWNKLGLINDTDCEKILKNAKFDIARIDEIEKTTKHDVIAFLTSVSES
LGEESRFVHYAMTSSDCIDTAVALQIKESLELILEDVSLLLEVIKKRALEHKNTLMVGRSHGIHGEPITFGLVLAIWYDE
ILHAKELLEHAKEVISYGKISGAMGNFAHAPLEFEEEVCKNLGLKAAPVSNQVIQRDRYAQVISAIAILASSCEQIAVAI
RHFQRTEVYEAEEYFSVGQKGSSAMPHKRNPVLSENITGLCRVLRSFVTPALENVALWHERDISHSSVERFILPDAFITA
DFMLMRLTNLIDKLLVYPENMMKNLNLTGGLVFSGRVLLELPFKGISREEAYKIVQRNAMKVWADLQNGKAAINEKNESL
FLLALLSDEDLRKSLSEEDVRKCFDYNYYTKNVDVIFKRTFK

Sequences:

>Translated_442_residues
MVERYSREIMAKKWDMQAKYDAWLKVELAAVKAWNKLGLINDTDCEKILKNAKFDIARIDEIEKTTKHDVIAFLTSVSES
LGEESRFVHYAMTSSDCIDTAVALQIKESLELILEDVSLLLEVIKKRALEHKNTLMVGRSHGIHGEPITFGLVLAIWYDE
ILHAKELLEHAKEVISYGKISGAMGNFAHAPLEFEEEVCKNLGLKAAPVSNQVIQRDRYAQVISAIAILASSCEQIAVAI
RHFQRTEVYEAEEYFSVGQKGSSAMPHKRNPVLSENITGLCRVLRSFVTPALENVALWHERDISHSSVERFILPDAFITA
DFMLMRLTNLIDKLLVYPENMMKNLNLTGGLVFSGRVLLELPFKGISREEAYKIVQRNAMKVWADLQNGKAAINEKNESL
FLLALLSDEDLRKSLSEEDVRKCFDYNYYTKNVDVIFKRTFK
>Mature_442_residues
MVERYSREIMAKKWDMQAKYDAWLKVELAAVKAWNKLGLINDTDCEKILKNAKFDIARIDEIEKTTKHDVIAFLTSVSES
LGEESRFVHYAMTSSDCIDTAVALQIKESLELILEDVSLLLEVIKKRALEHKNTLMVGRSHGIHGEPITFGLVLAIWYDE
ILHAKELLEHAKEVISYGKISGAMGNFAHAPLEFEEEVCKNLGLKAAPVSNQVIQRDRYAQVISAIAILASSCEQIAVAI
RHFQRTEVYEAEEYFSVGQKGSSAMPHKRNPVLSENITGLCRVLRSFVTPALENVALWHERDISHSSVERFILPDAFITA
DFMLMRLTNLIDKLLVYPENMMKNLNLTGGLVFSGRVLLELPFKGISREEAYKIVQRNAMKVWADLQNGKAAINEKNESL
FLLALLSDEDLRKSLSEEDVRKCFDYNYYTKNVDVIFKRTFK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=411, Percent_Identity=26.0340632603406, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4557269, Length=392, Percent_Identity=25.2551020408163, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1787376, Length=281, Percent_Identity=30.9608540925267, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508577, Length=340, Percent_Identity=26.4705882352941, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32564234, Length=305, Percent_Identity=27.8688524590164, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=390, Percent_Identity=26.6666666666667, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24647570, Length=404, Percent_Identity=24.2574257425743, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50283; Mature: 50283

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERYSREIMAKKWDMQAKYDAWLKVELAAVKAWNKLGLINDTDCEKILKNAKFDIARID
CCCHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHH
EIEKTTKHDVIAFLTSVSESLGEESRFVHYAMTSSDCIDTAVALQIKESLELILEDVSLL
HHHHHHHHHHHHHHHHHHHHHCCHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEVIKKRALEHKNTLMVGRSHGIHGEPITFGLVLAIWYDEILHAKELLEHAKEVISYGKI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNFAHAPLEFEEEVCKNLGLKAAPVSNQVIQRDRYAQVISAIAILASSCEQIAVAI
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHFQRTEVYEAEEYFSVGQKGSSAMPHKRNPVLSENITGLCRVLRSFVTPALENVALWHE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERFILPDAFITADFMLMRLTNLIDKLLVYPENMMKNLNLTGGLVFSGRVLLE
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEECEEEEE
LPFKGISREEAYKIVQRNAMKVWADLQNGKAAINEKNESLFLLALLSDEDLRKSLSEEDV
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEECCHHHHHHCCHHHH
RKCFDYNYYTKNVDVIFKRTFK
HHHHCCCCEECCCEEEEEECCC
>Mature Secondary Structure
MVERYSREIMAKKWDMQAKYDAWLKVELAAVKAWNKLGLINDTDCEKILKNAKFDIARID
CCCHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHH
EIEKTTKHDVIAFLTSVSESLGEESRFVHYAMTSSDCIDTAVALQIKESLELILEDVSLL
HHHHHHHHHHHHHHHHHHHHHCCHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEVIKKRALEHKNTLMVGRSHGIHGEPITFGLVLAIWYDEILHAKELLEHAKEVISYGKI
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNFAHAPLEFEEEVCKNLGLKAAPVSNQVIQRDRYAQVISAIAILASSCEQIAVAI
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHFQRTEVYEAEEYFSVGQKGSSAMPHKRNPVLSENITGLCRVLRSFVTPALENVALWHE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERFILPDAFITADFMLMRLTNLIDKLLVYPENMMKNLNLTGGLVFSGRVLLE
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEECEEEEE
LPFKGISREEAYKIVQRNAMKVWADLQNGKAAINEKNESLFLLALLSDEDLRKSLSEEDV
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEECCHHHHHHCCHHHH
RKCFDYNYYTKNVDVIFKRTFK
HHHHCCCCEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]