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The map label for this gene is ilvD [H]

Identifier: 57236926

GI number: 57236926

Start: 15807

End: 17483

Strand: Direct

Name: ilvD [H]

Synonym: CJE0012

Alternate gene names: 57236926

Gene position: 15807-17483 (Clockwise)

Preceding gene: 57236922

Following gene: 57236928

Centisome position: 0.89

GC content: 37.21

Gene sequence:

>1677_bases
ATGAGAAGCGATGCGATAAAAAAAGGACATTTAAAAGCACCTAATCGTTCTTTACTTCGTGCTTGTGGTTTGAAAGATGA
AGATTTTGACAAACCTTTTATAGGTGTAGCAAATAGCTATATAGATATAATCCCAGGACATTATTTTTTAAATAATTATG
CTAAAATCATTAAAGATGAGATATGCAAAAATGGTTGTGTGCCTTTTGAGTTTAATACCATAGGTGTAGATGATGGTATA
GCTATGGGACATGAAGGTATGCTTTATTCTTTACCAAGTCGTGAAATCATCGCAAATTCCATTGAAACAGTGATGAATGC
TCATCAACTTGATGCTTTAATCTGCATCCCAAACTGCGATAAAATCACTCCTGGTATGTTAATGGGAGCTTTAAGAGTAA
ATGTTCCAACCATTTTTGTAAGTGGTGGGCCTATGGCTTCAGGGGTAACTAAAAAAGGAGAAAAGATTAGTCTTAGCTCT
GTTTTTGAAGCTGTAGGGGCTTATGAAGCCAAAAAAATTAGCGAAGAAGAATTTAAAGATATAGAATGTTCAGCCTGTCC
AAGTGGTGGATCTTGCTCGGGAATGTTTACAGCAAATTCTATGAATACCTTATGCGAAGCTATGGGTATAGCACTAGAAG
GTAATGGAACCATCTTAGCTTTAAGCAAAGAAAGAGAAGAGCTTTTAAGAAAAGCAGCGCGTCGTATTTGTGAAATCGCC
TTAGATGAGCGTTTTAAAATTCGCAATATCATCACTCAAAAAGCCGTGCGTAATGCTATGGTAGTAGATATGGCAATGGG
TGGAAGTAGCAATACTGTTTTACACATGCTTGCCATTTCGCGTGAAGCAGGGGTGGCACTAGATATTAAAGATTTAAATT
TCATCTCAAGCAAAGTTGCACACATAGCAAAAATAGCCCCGTCGCTAAATAGTGTTTATATGGATGATATCCACAAAGCA
GGCGGTGTGAGTGCAGTTATGGCTGAAATTTCAAGCAGACAAGGACATATTTTAGAACTTGATGCACTGACCATTACAGG
AGAAAGTCTCAAAGAACGCTTAAAAAATGCCAAAATCAAAGATGAAAATATCATTCGTAAAGTAGATAATGCTTACTCAA
AAGTAGGTGGGCTTGCCATTTTGTTTGGAAATTTAGCCGAGCAAGGTTGCGTTATAAAAACCGCAGGAATAATCGGAGAA
AGAAAATTTAAAGGCAAAGCTGTTTGTTTTAACTCTCAAGATGAAGCTATAAAAGGCATTATCAAAGGCAAGGTGCAAAA
AGGAAATGTTTGTGTAATACGCTATGAAGGTCCAAAAGGGGGTCCTGGTATGCAAGAAATGTTAAGTCCTACTTCACTTT
TAATGGGTATGGGTTTAGGTGCTGATGTAGCTTTAATCACCGATGGTCGTTTTAGCGGGGCTACAAGGGGTTTAAGCGTT
GGTCATATTTCCCCTGAAGCAGCTGAAGGTGGTCTTATAGGACTTTTAAAAGATGGAGATGAAATAGAAATTGATGTAGA
TGCTTATACTATCCATGCAAATGTAAGTGAAGAAGAAATTGCTAAACGTAAAAAAGAATTTGCATTGCCTCAAAAAGAGG
TGTCTTCAAGATGGCTTAGAATGTATCAAAAACTTGTTAGCAATGCTAGTAAAGGTGCGGTTTTAGATATGGAATGA

Upstream 100 bases:

>100_bases
TAATAATTTTTATCATTTATGAAAATGTATATCAAAATAAATTTCACAAAAATTAAGCTTTGCTAAATATAATTTTAAAA
AATTATTATCAGGAGTTACA

Downstream 100 bases:

>100_bases
AAGGTTTCCTTTCATTCTTCTATGTCTTTGGTTATACGATTTCTTATATCTCTACGAACTATTTCCATGCCCCACAGCCA
AACAATATGGCCAAAAATTT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MRSDAIKKGHLKAPNRSLLRACGLKDEDFDKPFIGVANSYIDIIPGHYFLNNYAKIIKDEICKNGCVPFEFNTIGVDDGI
AMGHEGMLYSLPSREIIANSIETVMNAHQLDALICIPNCDKITPGMLMGALRVNVPTIFVSGGPMASGVTKKGEKISLSS
VFEAVGAYEAKKISEEEFKDIECSACPSGGSCSGMFTANSMNTLCEAMGIALEGNGTILALSKEREELLRKAARRICEIA
LDERFKIRNIITQKAVRNAMVVDMAMGGSSNTVLHMLAISREAGVALDIKDLNFISSKVAHIAKIAPSLNSVYMDDIHKA
GGVSAVMAEISSRQGHILELDALTITGESLKERLKNAKIKDENIIRKVDNAYSKVGGLAILFGNLAEQGCVIKTAGIIGE
RKFKGKAVCFNSQDEAIKGIIKGKVQKGNVCVIRYEGPKGGPGMQEMLSPTSLLMGMGLGADVALITDGRFSGATRGLSV
GHISPEAAEGGLIGLLKDGDEIEIDVDAYTIHANVSEEEIAKRKKEFALPQKEVSSRWLRMYQKLVSNASKGAVLDME

Sequences:

>Translated_558_residues
MRSDAIKKGHLKAPNRSLLRACGLKDEDFDKPFIGVANSYIDIIPGHYFLNNYAKIIKDEICKNGCVPFEFNTIGVDDGI
AMGHEGMLYSLPSREIIANSIETVMNAHQLDALICIPNCDKITPGMLMGALRVNVPTIFVSGGPMASGVTKKGEKISLSS
VFEAVGAYEAKKISEEEFKDIECSACPSGGSCSGMFTANSMNTLCEAMGIALEGNGTILALSKEREELLRKAARRICEIA
LDERFKIRNIITQKAVRNAMVVDMAMGGSSNTVLHMLAISREAGVALDIKDLNFISSKVAHIAKIAPSLNSVYMDDIHKA
GGVSAVMAEISSRQGHILELDALTITGESLKERLKNAKIKDENIIRKVDNAYSKVGGLAILFGNLAEQGCVIKTAGIIGE
RKFKGKAVCFNSQDEAIKGIIKGKVQKGNVCVIRYEGPKGGPGMQEMLSPTSLLMGMGLGADVALITDGRFSGATRGLSV
GHISPEAAEGGLIGLLKDGDEIEIDVDAYTIHANVSEEEIAKRKKEFALPQKEVSSRWLRMYQKLVSNASKGAVLDME
>Mature_558_residues
MRSDAIKKGHLKAPNRSLLRACGLKDEDFDKPFIGVANSYIDIIPGHYFLNNYAKIIKDEICKNGCVPFEFNTIGVDDGI
AMGHEGMLYSLPSREIIANSIETVMNAHQLDALICIPNCDKITPGMLMGALRVNVPTIFVSGGPMASGVTKKGEKISLSS
VFEAVGAYEAKKISEEEFKDIECSACPSGGSCSGMFTANSMNTLCEAMGIALEGNGTILALSKEREELLRKAARRICEIA
LDERFKIRNIITQKAVRNAMVVDMAMGGSSNTVLHMLAISREAGVALDIKDLNFISSKVAHIAKIAPSLNSVYMDDIHKA
GGVSAVMAEISSRQGHILELDALTITGESLKERLKNAKIKDENIIRKVDNAYSKVGGLAILFGNLAEQGCVIKTAGIIGE
RKFKGKAVCFNSQDEAIKGIIKGKVQKGNVCVIRYEGPKGGPGMQEMLSPTSLLMGMGLGADVALITDGRFSGATRGLSV
GHISPEAAEGGLIGLLKDGDEIEIDVDAYTIHANVSEEEIAKRKKEFALPQKEVSSRWLRMYQKLVSNASKGAVLDME

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=608, Percent_Identity=46.546052631579, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1788157, Length=522, Percent_Identity=29.6934865900383, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1786464, Length=564, Percent_Identity=31.2056737588652, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI2367371, Length=570, Percent_Identity=31.2280701754386, Blast_Score=199, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6322476, Length=551, Percent_Identity=42.1052631578947, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 60008; Mature: 60008

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDAIKKGHLKAPNRSLLRACGLKDEDFDKPFIGVANSYIDIIPGHYFLNNYAKIIKDE
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHCCCCHHHCCCHHHHHHHHHHHHHH
ICKNGCVPFEFNTIGVDDGIAMGHEGMLYSLPSREIIANSIETVMNAHQLDALICIPNCD
HHHCCCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCC
KITPGMLMGALRVNVPTIFVSGGPMASGVTKKGEKISLSSVFEAVGAYEAKKISEEEFKD
CCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHCCHHHHCC
IECSACPSGGSCSGMFTANSMNTLCEAMGIALEGNGTILALSKEREELLRKAARRICEIA
CCCCCCCCCCCCCEEEECCHHHHHHHHHCEEEECCCEEEEECHHHHHHHHHHHHHHHHHH
LDERFKIRNIITQKAVRNAMVVDMAMGGSSNTVLHMLAISREAGVALDIKDLNFISSKVA
HCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHH
HIAKIAPSLNSVYMDDIHKAGGVSAVMAEISSRQGHILELDALTITGESLKERLKNAKIK
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHCCCCC
DENIIRKVDNAYSKVGGLAILFGNLAEQGCVIKTAGIIGERKFKGKAVCFNSQDEAIKGI
CHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEECCCCCCCCCCCCEEEECCHHHHHHHH
IKGKVQKGNVCVIRYEGPKGGPGMQEMLSPTSLLMGMGLGADVALITDGRFSGATRGLSV
HHCCCCCCCEEEEEECCCCCCCCHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCCCEE
GHISPEAAEGGLIGLLKDGDEIEIDVDAYTIHANVSEEEIAKRKKEFALPQKEVSSRWLR
CCCCCCCCCCCEEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHH
MYQKLVSNASKGAVLDME
HHHHHHCCCCCCCEEECC
>Mature Secondary Structure
MRSDAIKKGHLKAPNRSLLRACGLKDEDFDKPFIGVANSYIDIIPGHYFLNNYAKIIKDE
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHCCCCHHHCCCHHHHHHHHHHHHHH
ICKNGCVPFEFNTIGVDDGIAMGHEGMLYSLPSREIIANSIETVMNAHQLDALICIPNCD
HHHCCCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCC
KITPGMLMGALRVNVPTIFVSGGPMASGVTKKGEKISLSSVFEAVGAYEAKKISEEEFKD
CCCHHHHHHHEEECCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHCCHHHHCC
IECSACPSGGSCSGMFTANSMNTLCEAMGIALEGNGTILALSKEREELLRKAARRICEIA
CCCCCCCCCCCCCEEEECCHHHHHHHHHCEEEECCCEEEEECHHHHHHHHHHHHHHHHHH
LDERFKIRNIITQKAVRNAMVVDMAMGGSSNTVLHMLAISREAGVALDIKDLNFISSKVA
HCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHH
HIAKIAPSLNSVYMDDIHKAGGVSAVMAEISSRQGHILELDALTITGESLKERLKNAKIK
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHCCCCC
DENIIRKVDNAYSKVGGLAILFGNLAEQGCVIKTAGIIGERKFKGKAVCFNSQDEAIKGI
CHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEECCCCCCCCCCCCEEEECCHHHHHHHH
IKGKVQKGNVCVIRYEGPKGGPGMQEMLSPTSLLMGMGLGADVALITDGRFSGATRGLSV
HHCCCCCCCEEEEEECCCCCCCCHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCCCEE
GHISPEAAEGGLIGLLKDGDEIEIDVDAYTIHANVSEEEIAKRKKEFALPQKEVSSRWLR
CCCCCCCCCCCEEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHH
MYQKLVSNASKGAVLDME
HHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA