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The map label for this gene is gltD [H]

Identifier: 57236922

GI number: 57236922

Start: 12642

End: 14087

Strand: Direct

Name: gltD [H]

Synonym: CJE0008

Alternate gene names: 57236922

Gene position: 12642-14087 (Clockwise)

Preceding gene: 57236921

Following gene: 57236926

Centisome position: 0.71

GC content: 34.79

Gene sequence:

>1446_bases
ATGGGAAATGCAAGAGGTTTTTTAGACTTTAAAAGAATGGATTTTAAAAAAATTGCTCCTAAGGAAAGGGTTTTAAATTT
CAAAGAATTTACTATACCTTTGGATAAAAAAGAACAAGAAATTCAAGGTGGACGATGTATGGATTGTGGTGTGGCCTTTT
GTCATACGGGTGTGATGAGCGAAGGAAAAGATGTGGGCTGTCCTTTAAACAATCTCATTCCTGAATGGAACGATCTTGTT
TATCGTTCTTTATGGAAAGAAGCTTATGAAAGACTTGATCTTACCAATCCTTTTCCTGAATTTACCGGTCGTGTTTGCCC
TGCTCCTTGTGAGGATTCTTGTGTGTGTGCGATAAATGGTGTTAGTGTGAGCATAAAAAATAATGAATTATCCATTATAG
AAAATGCTTTTAAGGAAAATTTAGTAAGAGTAAATAAACCTAAGCAATACAATGGCAAAAAAATCGCTGTCATAGGAAGT
GGGCCTGCTGGTCTTGCTTGTGCTAATACTTTAAATTCACTAGGTTATAAAGTAAGTGTTTTTGAAAGAAGCGATAAAAT
AGGCGGACTTTTGATGTATGGCATCCCTGATATGAAGCTTGATAAAAGTATAGTTCAAAGACGGGTGGATTTGCTTAAAA
AAAGTGGGATTGAATTTAAAGTGAATGAAAATATAGACAGCAGAGATAAGGTTTCAAAACTTCTAAAAGAATTTGATGCT
TTGGTGCTTTGTACAGGAGCAAGTAAGCCTATAGATCTTGATATAGAAGGTAGAAAGCTAAAAGGGGTTGAATTTGCCCT
TGATTTTTTAACGCAAAATACCAAAACTTTGTTAAAAACAGGCAAGGGTGCAGATACGGCCAAAGGTAAAAATGTCTTGG
TTATAGGAAGCGGGGATACAAGTGTGGATTGTATCGCTGTAGCGACAAGACAAGGGGCTAAATCTATAGTGCGTTTTGAA
AGAAGTCCAAAAAGACCTTTGCAAAGAAGCCAAAACAATCCTTGGCCTTTAAAAGCTGACATTTTTACTACTGATTATGG
TTTAGAAGAAGCCATTGCTGTATATGGCAAAGATCCTAGGGAATATCAAAAAATGACAAAGAAATTTTTAGGAAAAACTC
ATGTAGAAGGTGTTGAAGCAAATGATTTAAAACGCGAGTTTAAAGAAGGCAAAGCTATAAATGTCGAAATTTCAAATTCT
AAAAAAACTTATAAAGCGGATTTAGTGCTTTTAGCTATGGGTTTTAGCGGGTGCGAAGAAGCTATCTCTAAAAATTTTGG
AGTGAAATTAGATGAGAAAAACAACATAAGCACAGAAAATTTCCAAACCACTCATAAAAAAATCTTTGCTTGTGGAGATG
CTAGAAAAGGACAATCTTTAGTTGTTTGGGCTATAAAAGATGGTATAGAATGTGCTTTGAGTTTGCATCAAAATTTAGCA
AAATAA

Upstream 100 bases:

>100_bases
AGATTATGAAAAGATGTTAAAAATGCTTGATCTTTGTAAAAATGAAAAAGATCCAAATTTAGCAGCATTTTTGAAAATCA
CACAAAAATAAGGAGTAAAA

Downstream 100 bases:

>100_bases
TCATAAAAGTTTAAAACTTTTTCGGTGGAGTTTGCAAATTCCAAATTTAGCTATAAATTCTTTATGAGCTTTTGTGCCAT
AGGCTTTGTTTTTTTCAAAT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MGNARGFLDFKRMDFKKIAPKERVLNFKEFTIPLDKKEQEIQGGRCMDCGVAFCHTGVMSEGKDVGCPLNNLIPEWNDLV
YRSLWKEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAINGVSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAVIGS
GPAGLACANTLNSLGYKVSVFERSDKIGGLLMYGIPDMKLDKSIVQRRVDLLKKSGIEFKVNENIDSRDKVSKLLKEFDA
LVLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADTAKGKNVLVIGSGDTSVDCIAVATRQGAKSIVRFE
RSPKRPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPREYQKMTKKFLGKTHVEGVEANDLKREFKEGKAINVEISNS
KKTYKADLVLLAMGFSGCEEAISKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLA
K

Sequences:

>Translated_481_residues
MGNARGFLDFKRMDFKKIAPKERVLNFKEFTIPLDKKEQEIQGGRCMDCGVAFCHTGVMSEGKDVGCPLNNLIPEWNDLV
YRSLWKEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAINGVSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAVIGS
GPAGLACANTLNSLGYKVSVFERSDKIGGLLMYGIPDMKLDKSIVQRRVDLLKKSGIEFKVNENIDSRDKVSKLLKEFDA
LVLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADTAKGKNVLVIGSGDTSVDCIAVATRQGAKSIVRFE
RSPKRPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPREYQKMTKKFLGKTHVEGVEANDLKREFKEGKAINVEISNS
KKTYKADLVLLAMGFSGCEEAISKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLA
K
>Mature_480_residues
GNARGFLDFKRMDFKKIAPKERVLNFKEFTIPLDKKEQEIQGGRCMDCGVAFCHTGVMSEGKDVGCPLNNLIPEWNDLVY
RSLWKEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAINGVSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAVIGSG
PAGLACANTLNSLGYKVSVFERSDKIGGLLMYGIPDMKLDKSIVQRRVDLLKKSGIEFKVNENIDSRDKVSKLLKEFDAL
VLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADTAKGKNVLVIGSGDTSVDCIAVATRQGAKSIVRFER
SPKRPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPREYQKMTKKFLGKTHVEGVEANDLKREFKEGKAINVEISNSK
KTYKADLVLLAMGFSGCEEAISKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLAK

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=293, Percent_Identity=24.9146757679181, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=32.7800829875519, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788811, Length=479, Percent_Identity=30.6889352818372, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI87082180, Length=468, Percent_Identity=30.7692307692308, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1788468, Length=441, Percent_Identity=26.530612244898, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789243, Length=449, Percent_Identity=25.8351893095768, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17570289, Length=488, Percent_Identity=42.0081967213115, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71984108, Length=475, Percent_Identity=23.7894736842105, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17543860, Length=183, Percent_Identity=28.4153005464481, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=484, Percent_Identity=44.4214876033058, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI28574881, Length=473, Percent_Identity=46.3002114164905, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665539, Length=473, Percent_Identity=46.3002114164905, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665547, Length=480, Percent_Identity=45.8333333333333, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665543, Length=480, Percent_Identity=45.8333333333333, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24640763, Length=294, Percent_Identity=26.530612244898, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18858217, Length=294, Percent_Identity=26.530612244898, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53330; Mature: 53199

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNARGFLDFKRMDFKKIAPKERVLNFKEFTIPLDKKEQEIQGGRCMDCGVAFCHTGVMS
CCCCCCCCHHHHCCHHHCCCHHHHCCHHHEEECCCCCHHHHCCCCCHHHHHHHHHHCCCC
EGKDVGCPLNNLIPEWNDLVYRSLWKEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAING
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEEC
VSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAVIGSGPAGLACANTLNSLGYKVSV
EEEEEECCCHHHHHHHHHHCCEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEE
FERSDKIGGLLMYGIPDMKLDKSIVQRRVDLLKKSGIEFKVNENIDSRDKVSKLLKEFDA
EECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCE
LVLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADTAKGKNVLVIGSGDT
EEEEECCCCCEEEEECCCEECCHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEECCCC
SVDCIAVATRQGAKSIVRFERSPKRPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPR
CEEEEEEEHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCCHH
EYQKMTKKFLGKTHVEGVEANDLKREFKEGKAINVEISNSKKTYKADLVLLAMGFSGCEE
HHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEEECCHHHHH
AISKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLA
HHHCCCCEEECCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHC
K
C
>Mature Secondary Structure 
GNARGFLDFKRMDFKKIAPKERVLNFKEFTIPLDKKEQEIQGGRCMDCGVAFCHTGVMS
CCCCCCCHHHHCCHHHCCCHHHHCCHHHEEECCCCCHHHHCCCCCHHHHHHHHHHCCCC
EGKDVGCPLNNLIPEWNDLVYRSLWKEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAING
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEEC
VSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAVIGSGPAGLACANTLNSLGYKVSV
EEEEEECCCHHHHHHHHHHCCEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEE
FERSDKIGGLLMYGIPDMKLDKSIVQRRVDLLKKSGIEFKVNENIDSRDKVSKLLKEFDA
EECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCE
LVLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADTAKGKNVLVIGSGDT
EEEEECCCCCEEEEECCCEECCHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEECCCC
SVDCIAVATRQGAKSIVRFERSPKRPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPR
CEEEEEEEHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCCHH
EYQKMTKKFLGKTHVEGVEANDLKREFKEGKAINVEISNSKKTYKADLVLLAMGFSGCEE
HHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEEECCHHHHH
AISKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLA
HHHCCCCEEECCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]