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The map label for this gene is trpS [H]

Identifier: 21227573

GI number: 21227573

Start: 1754232

End: 1755719

Strand: Reverse

Name: trpS [H]

Synonym: MM_1471

Alternate gene names: 21227573

Gene position: 1755719-1754232 (Counterclockwise)

Preceding gene: 21227576

Following gene: 21227572

Centisome position: 42.86

GC content: 47.65

Gene sequence:

>1488_bases
ATGAGGGACATTATGGATAATAAACTTGACCCGTGGAGTTCAAGCAATATCACTGACTACTCCAAGTTATTCGAAGAATT
CGGGATTTCTCCTTTTGAAAATATACTTCCGGAAATTCCGTCCCCGCATAAGTACATGCGGCGGAGAATCATCTTCGGGC
ACCGTGATTATGAACAGATTGCAGAGGCCATGCGGACAGGTGCCCCATTTTCAGTTATGGACGGCTTTATGCCATCAGGA
AAAGTCCATCTGGGGCACAAAATGGTAATGGACCAGATCGTCTGGCACCAGGAGAAGGGAGCTTCAGCCTTTGTCGGGAT
TGCGGACAGGGAAGCCTTTTCAGTGCGCGGCTTTTCCTGGCAGAAATGCAGGGAAATAGGGGTAGAAGAATATATATTGA
GCCTCATTGCCCTCGGTTTCAAGCCTGAAGGTCTCATTTACTTCCAGTCGGGATGCGGAAGTGTTAAAGACCTGGCATTC
GAACTGGGAGCAAAAGTCAATTTCTCGGAACTGAGCGCTATCTATGGCTTTTCAGGTGAAACAAGCCTCTCCCACATGCT
CAGCGTAGCAACCCAGGCTGCAGACATCCTCCAGCCCCAGCTAGAGGAATTCGGAGGATCAAAACCCGTTGTTGTTCCCG
TGGGCCCCGACCAGGACCCTCACCTCCGCCTTACAAGAGGACTCGCAGGCAAAATGAATATGTTCAGGGTAGAGGAAAGG
GAAACAGCAGAAGGCGGAAAGTACCTCAGCGTCCGGGGAAAAGGAGCTCCCAGGGAAGCTTTACAGGAACTTAAAAAACG
CATTCCAGGGAAAGTAAAACTCTATGAAGAACACATTGATGTGCTCCAGACTCCTGACTATGCATTCCTTGAAAGGTTTG
TTTTACAGATAAACCAGCCTGAGAGAAGGAATTATTTCATGACCCAGATTCAGGAAATCGCCACATTTGCCAATGCGGCT
AAACCACCTGAATTTTCGGAGTATGAAAAATACTTCGTTTTTAAAAGGGTCTGGAAAACCACAGGGAAAATCCTTGAAGA
AGTCGTGGCAGAAGTTGCAGCTGAGTTCGATGGCTACGCTTTCATTCCTCCTGCATCAACTTACCACCGTTTCATGAGCG
GGCTACAGGGAGGAAAGATGTCGAGCAGCATCCCTGACAGCCATATAGCCCTCACAGATGACCCTAAGGAAGGGGCAAAA
AAGGTAAAGAGGGCAAAGACCGGAGGCTGCATAACCCTTGAAGAGCAGAAAAAACTGGGTGGGAAACCTGATGAATGTTC
TGTCTTTGAACTGATGCTCTTCCACCTGATAGAAGATGATGAGGAACTGCTGGAAATCAGAGAGGAATGTATCTACGGAA
CCAGGATGTGCGGCTCATGCAAGCAGCTCGCAGCCGAAAAGATGCAGGAGTTCCTTAAAGATCACCAGGAGAAGAGGGAA
CTTGCAAGAGAACAGCTTGATGAATACAGAATCATCTATAAAAAATAA

Upstream 100 bases:

>100_bases
TTAAAGAGCGATTTAACCGGAACAAAGCTTACGCTTTATTTATGCTACCCTTATTTAGCAAGAAAAAACTGCCTGCAGTG
TTTTCCGGGAACGGCAGCCC

Downstream 100 bases:

>100_bases
ATCACCCATCAGGTCATGAATCTTTAGTAAATTATGTATCTTCAGTAGATCCATGAACCTTCAGTAAATATTTATCTCCA
GTAGACCCATGAATCTTTAG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRDIMDNKLDPWSSSNITDYSKLFEEFGISPFENILPEIPSPHKYMRRRIIFGHRDYEQIAEAMRTGAPFSVMDGFMPSG
KVHLGHKMVMDQIVWHQEKGASAFVGIADREAFSVRGFSWQKCREIGVEEYILSLIALGFKPEGLIYFQSGCGSVKDLAF
ELGAKVNFSELSAIYGFSGETSLSHMLSVATQAADILQPQLEEFGGSKPVVVPVGPDQDPHLRLTRGLAGKMNMFRVEER
ETAEGGKYLSVRGKGAPREALQELKKRIPGKVKLYEEHIDVLQTPDYAFLERFVLQINQPERRNYFMTQIQEIATFANAA
KPPEFSEYEKYFVFKRVWKTTGKILEEVVAEVAAEFDGYAFIPPASTYHRFMSGLQGGKMSSSIPDSHIALTDDPKEGAK
KVKRAKTGGCITLEEQKKLGGKPDECSVFELMLFHLIEDDEELLEIREECIYGTRMCGSCKQLAAEKMQEFLKDHQEKRE
LAREQLDEYRIIYKK

Sequences:

>Translated_495_residues
MRDIMDNKLDPWSSSNITDYSKLFEEFGISPFENILPEIPSPHKYMRRRIIFGHRDYEQIAEAMRTGAPFSVMDGFMPSG
KVHLGHKMVMDQIVWHQEKGASAFVGIADREAFSVRGFSWQKCREIGVEEYILSLIALGFKPEGLIYFQSGCGSVKDLAF
ELGAKVNFSELSAIYGFSGETSLSHMLSVATQAADILQPQLEEFGGSKPVVVPVGPDQDPHLRLTRGLAGKMNMFRVEER
ETAEGGKYLSVRGKGAPREALQELKKRIPGKVKLYEEHIDVLQTPDYAFLERFVLQINQPERRNYFMTQIQEIATFANAA
KPPEFSEYEKYFVFKRVWKTTGKILEEVVAEVAAEFDGYAFIPPASTYHRFMSGLQGGKMSSSIPDSHIALTDDPKEGAK
KVKRAKTGGCITLEEQKKLGGKPDECSVFELMLFHLIEDDEELLEIREECIYGTRMCGSCKQLAAEKMQEFLKDHQEKRE
LAREQLDEYRIIYKK
>Mature_495_residues
MRDIMDNKLDPWSSSNITDYSKLFEEFGISPFENILPEIPSPHKYMRRRIIFGHRDYEQIAEAMRTGAPFSVMDGFMPSG
KVHLGHKMVMDQIVWHQEKGASAFVGIADREAFSVRGFSWQKCREIGVEEYILSLIALGFKPEGLIYFQSGCGSVKDLAF
ELGAKVNFSELSAIYGFSGETSLSHMLSVATQAADILQPQLEEFGGSKPVVVPVGPDQDPHLRLTRGLAGKMNMFRVEER
ETAEGGKYLSVRGKGAPREALQELKKRIPGKVKLYEEHIDVLQTPDYAFLERFVLQINQPERRNYFMTQIQEIATFANAA
KPPEFSEYEKYFVFKRVWKTTGKILEEVVAEVAAEFDGYAFIPPASTYHRFMSGLQGGKMSSSIPDSHIALTDDPKEGAK
KVKRAKTGGCITLEEQKKLGGKPDECSVFELMLFHLIEDDEELLEIREECIYGTRMCGSCKQLAAEKMQEFLKDHQEKRE
LAREQLDEYRIIYKK

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI47419920, Length=245, Percent_Identity=26.9387755102041, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI47419918, Length=245, Percent_Identity=26.9387755102041, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI47419916, Length=245, Percent_Identity=26.9387755102041, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI47419914, Length=245, Percent_Identity=26.9387755102041, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI72001474, Length=247, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324475, Length=263, Percent_Identity=24.7148288973384, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17864462, Length=248, Percent_Identity=26.6129032258064, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645295, Length=248, Percent_Identity=26.6129032258064, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- InterPro:   IPR020653 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 56244; Mature: 56244

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIMDNKLDPWSSSNITDYSKLFEEFGISPFENILPEIPSPHKYMRRRIIFGHRDYEQI
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHCCCCCHHHH
AEAMRTGAPFSVMDGFMPSGKVHLGHKMVMDQIVWHQEKGASAFVGIADREAFSVRGFSW
HHHHHCCCCHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEECCCCHHEECCCCH
QKCREIGVEEYILSLIALGFKPEGLIYFQSGCGSVKDLAFELGAKVNFSELSAIYGFSGE
HHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCC
TSLSHMLSVATQAADILQPQLEEFGGSKPVVVPVGPDQDPHLRLTRGLAGKMNMFRVEER
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHCCCCCCHHEEEHHH
ETAEGGKYLSVRGKGAPREALQELKKRIPGKVKLYEEHIDVLQTPDYAFLERFVLQINQP
HHCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHHHHHCCCC
ERRNYFMTQIQEIATFANAAKPPEFSEYEKYFVFKRVWKTTGKILEEVVAEVAAEFDGYA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
FIPPASTYHRFMSGLQGGKMSSSIPDSHIALTDDPKEGAKKVKRAKTGGCITLEEQKKLG
ECCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCEEEEHHHHHCC
GKPDECSVFELMLFHLIEDDEELLEIREECIYGTRMCGSCKQLAAEKMQEFLKDHQEKRE
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAREQLDEYRIIYKK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MRDIMDNKLDPWSSSNITDYSKLFEEFGISPFENILPEIPSPHKYMRRRIIFGHRDYEQI
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHCCCCCHHHH
AEAMRTGAPFSVMDGFMPSGKVHLGHKMVMDQIVWHQEKGASAFVGIADREAFSVRGFSW
HHHHHCCCCHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEECCCCHHEECCCCH
QKCREIGVEEYILSLIALGFKPEGLIYFQSGCGSVKDLAFELGAKVNFSELSAIYGFSGE
HHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCC
TSLSHMLSVATQAADILQPQLEEFGGSKPVVVPVGPDQDPHLRLTRGLAGKMNMFRVEER
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHCCCCCCHHEEEHHH
ETAEGGKYLSVRGKGAPREALQELKKRIPGKVKLYEEHIDVLQTPDYAFLERFVLQINQP
HHCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHHHHHCCCC
ERRNYFMTQIQEIATFANAAKPPEFSEYEKYFVFKRVWKTTGKILEEVVAEVAAEFDGYA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
FIPPASTYHRFMSGLQGGKMSSSIPDSHIALTDDPKEGAKKVKRAKTGGCITLEEQKKLG
ECCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCEEEEHHHHHCC
GKPDECSVFELMLFHLIEDDEELLEIREECIYGTRMCGSCKQLAAEKMQEFLKDHQEKRE
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAREQLDEYRIIYKK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]