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The map label for this gene is pheS

Identifier: 21227572

GI number: 21227572

Start: 1752535

End: 1754022

Strand: Reverse

Name: pheS

Synonym: MM_1470

Alternate gene names: 21227572

Gene position: 1754022-1752535 (Counterclockwise)

Preceding gene: 21227573

Following gene: 21227570

Centisome position: 42.82

GC content: 49.53

Gene sequence:

>1488_bases
TTGACAGTTATGAGTATTCAGGACAACCTCACAATTAACGAAAAAAAAGTCTTGCTCGCCCTTGAGGAACTTAGATCGGC
AGTTCCGGATAAGCTGGAAGAGAAGTCAGGGCTGCAGATAGATGCGGCGATGCAGGCTGCTTTCATGCTTCAGGAAAAAG
AGCTTGCTTCTGTTTCCGAAAAAGTTCTCGAGCGCTATTCCCTTACAAAGGAAGGAGAAGAATACAAAAAAACAGGGCTT
CCAGAGCGCCAGATTATTGATGCCCTGAAAAACCCTGTCTCCCTTGAGGAGCTCAGGAGCTGTTTCTCCCCCCAGACCGT
GGGAATTGCAACGGGCTGGCTCGTAAAAAAAGGATGGGCAAAGGTTGAAAACGGAATAATGGTTCCTTCCGGAGAAGCTT
CTGAAGGTAAGGACGAAAAAGCCCTTTCAGCTTTTGCAGGAAAAGCAAAGACTCTCGAAGAACTTGGAGCCGATGAAGGG
ACCGTAAAAGACCTTCTCAAACGCAAACTCGTCGTAAAACACGAAGAAAAGTTCAGGACTGTTTCAATAACAGACGCAGG
AAGTGAACTTGCATCTCAGGGGCTTGTCCTTGAAGAAGAAATTGCCCAGCTTACTCCCGAACTGCTGAAAAGCGGAGCAT
GGAAAGAGAAAAAATTCAGGCCCTACAGGCTCGATATTACACCTAAACCACTTTACGGGACAAAAATTCACCCTTACAGG
CGCCTGATTGAACAGATGCGCCAGATCTTTCTGGAAATGGGCTTTACAGAGATAAAAGGCGGTATAATCCAGAGTTCTTT
CTGGAACTTCGACGCCCTCTTCCAGCCCCAGGACCACCCTGCCAGGGATATGCAGGACACATTCCACCTCGGCAGCACCT
GTCAGATCCCGGCTGAATATTCGGGAAAAGTGGCAGCAATGCATGAGAACGGAGGAGATATTGACTCCTGCGGCTGGGGA
GGAATATGGGACAGAGAACTTGCCGGGCGCAACGTTCTCAGGACTCACACCACCTCCGTGACCATAAAATATCTTGCAGA
CAACCCGGAGCCGCCAGTAAAAGCTTTCTGCATTGACAGGGCTTACCGCAGGGAAACAATTGACCCGACACACACGCCTG
AGTTCGAGCAGCTTGAAGGTGTGGTCATGGATAAGGATATGTCTTTTGCAGACCTTCTCGGCCTCCTTGCAGAGTTCTAC
CACAGGATGGGATTCGAAGAAGTCCGCTTCCGCCCTGGATATTTCCCATATACGGAACCAAGCGTGGAACCTGAAGTTTA
TGTAGACGGGCTCGGTTGGGTTGAACTAGGAGGCGCAGGCGTTTTCAGGAAAGAGGTCACAGAACCTCTGGGCATCAAAG
CGCCCGTCCTTGCCTGGGGGCTTGGAGTAAGCCGGCTCGCTATGCTAAAACTCGGGCTTAAAGACCTGAGGCTTCTTTAT
CAGTCTGATATTGACTGGCTCAGGAAGAGCGAAGTTTGCAAGATTTAA

Upstream 100 bases:

>100_bases
AATCTACAATAAATTCATCAAATACAAGTGCCAAACTTATATCAAAATCTTAGTATTAAAGTCTTAAATTTTCAGGACTC
ACAGGATATGGTTCACAGGA

Downstream 100 bases:

>100_bases
AAATAAAAAAAATCAAAAGTAATTCGGCTGTAAAAACTGTCTGTTCCAGCGAGCAGGCAGTTTCTTTCTTGTATTTTGAT
TTTTGTATTTTAATTTGTTT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTVMSIQDNLTINEKKVLLALEELRSAVPDKLEEKSGLQIDAAMQAAFMLQEKELASVSEKVLERYSLTKEGEEYKKTGL
PERQIIDALKNPVSLEELRSCFSPQTVGIATGWLVKKGWAKVENGIMVPSGEASEGKDEKALSAFAGKAKTLEELGADEG
TVKDLLKRKLVVKHEEKFRTVSITDAGSELASQGLVLEEEIAQLTPELLKSGAWKEKKFRPYRLDITPKPLYGTKIHPYR
RLIEQMRQIFLEMGFTEIKGGIIQSSFWNFDALFQPQDHPARDMQDTFHLGSTCQIPAEYSGKVAAMHENGGDIDSCGWG
GIWDRELAGRNVLRTHTTSVTIKYLADNPEPPVKAFCIDRAYRRETIDPTHTPEFEQLEGVVMDKDMSFADLLGLLAEFY
HRMGFEEVRFRPGYFPYTEPSVEPEVYVDGLGWVELGGAGVFRKEVTEPLGIKAPVLAWGLGVSRLAMLKLGLKDLRLLY
QSDIDWLRKSEVCKI

Sequences:

>Translated_495_residues
MTVMSIQDNLTINEKKVLLALEELRSAVPDKLEEKSGLQIDAAMQAAFMLQEKELASVSEKVLERYSLTKEGEEYKKTGL
PERQIIDALKNPVSLEELRSCFSPQTVGIATGWLVKKGWAKVENGIMVPSGEASEGKDEKALSAFAGKAKTLEELGADEG
TVKDLLKRKLVVKHEEKFRTVSITDAGSELASQGLVLEEEIAQLTPELLKSGAWKEKKFRPYRLDITPKPLYGTKIHPYR
RLIEQMRQIFLEMGFTEIKGGIIQSSFWNFDALFQPQDHPARDMQDTFHLGSTCQIPAEYSGKVAAMHENGGDIDSCGWG
GIWDRELAGRNVLRTHTTSVTIKYLADNPEPPVKAFCIDRAYRRETIDPTHTPEFEQLEGVVMDKDMSFADLLGLLAEFY
HRMGFEEVRFRPGYFPYTEPSVEPEVYVDGLGWVELGGAGVFRKEVTEPLGIKAPVLAWGLGVSRLAMLKLGLKDLRLLY
QSDIDWLRKSEVCKI
>Mature_494_residues
TVMSIQDNLTINEKKVLLALEELRSAVPDKLEEKSGLQIDAAMQAAFMLQEKELASVSEKVLERYSLTKEGEEYKKTGLP
ERQIIDALKNPVSLEELRSCFSPQTVGIATGWLVKKGWAKVENGIMVPSGEASEGKDEKALSAFAGKAKTLEELGADEGT
VKDLLKRKLVVKHEEKFRTVSITDAGSELASQGLVLEEEIAQLTPELLKSGAWKEKKFRPYRLDITPKPLYGTKIHPYRR
LIEQMRQIFLEMGFTEIKGGIIQSSFWNFDALFQPQDHPARDMQDTFHLGSTCQIPAEYSGKVAAMHENGGDIDSCGWGG
IWDRELAGRNVLRTHTTSVTIKYLADNPEPPVKAFCIDRAYRRETIDPTHTPEFEQLEGVVMDKDMSFADLLGLLAEFYH
RMGFEEVRFRPGYFPYTEPSVEPEVYVDGLGWVELGGAGVFRKEVTEPLGIKAPVLAWGLGVSRLAMLKLGLKDLRLLYQ
SDIDWLRKSEVCKI

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=472, Percent_Identity=36.0169491525424, Blast_Score=261, Evalue=1e-69,
Organism=Escherichia coli, GI1788007, Length=260, Percent_Identity=34.6153846153846, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32563657, Length=454, Percent_Identity=34.5814977973568, Blast_Score=257, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI17508957, Length=454, Percent_Identity=34.5814977973568, Blast_Score=257, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321087, Length=452, Percent_Identity=33.6283185840708, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI18858079, Length=348, Percent_Identity=39.367816091954, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_METMA (Q8PWV6)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633494.1
- ProteinModelPortal:   Q8PWV6
- SMR:   Q8PWV6
- GeneID:   1479812
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1470
- NMPDR:   fig|192952.1.peg.1470
- HOGENOM:   HBG497668
- OMA:   PEFYQCE
- ProtClustDB:   PRK04172
- BioCyc:   MMAZ192952:MM1470-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00282
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF01409 tRNA-synt_2d

EC number: =6.1.1.20

Molecular weight: Translated: 55607; Mature: 55476

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVMSIQDNLTINEKKVLLALEELRSAVPDKLEEKSGLQIDAAMQAAFMLQEKELASVSE
CEEEEECCCCEECHHHHHHHHHHHHHHCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHH
KVLERYSLTKEGEEYKKTGLPERQIIDALKNPVSLEELRSCFSPQTVGIATGWLVKKGWA
HHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEEHHHHHHHCHH
KVENGIMVPSGEASEGKDEKALSAFAGKAKTLEELGADEGTVKDLLKRKLVVKHEEKFRT
HHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEE
VSITDAGSELASQGLVLEEEIAQLTPELLKSGAWKEKKFRPYRLDITPKPLYGTKIHPYR
EEEECCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCHHH
RLIEQMRQIFLEMGFTEIKGGIIQSSFWNFDALFQPQDHPARDMQDTFHLGSTCQIPAEY
HHHHHHHHHHHHHCHHHHCCCHHHCCCCCCCEEECCCCCCCHHHHHHHCCCCEEECCCCC
SGKVAAMHENGGDIDSCGWGGIWDRELAGRNVLRTHTTSVTIKYLADNPEPPVKAFCIDR
CCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEECCEEEEEEECCCCCCCHHHHHHHH
AYRRETIDPTHTPEFEQLEGVVMDKDMSFADLLGLLAEFYHRMGFEEVRFRPGYFPYTEP
HHHHCCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
SVEPEVYVDGLGWVELGGAGVFRKEVTEPLGIKAPVLAWGLGVSRLAMLKLGLKDLRLLY
CCCCCEEECCCCEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHH
QSDIDWLRKSEVCKI
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVMSIQDNLTINEKKVLLALEELRSAVPDKLEEKSGLQIDAAMQAAFMLQEKELASVSE
EEEEECCCCEECHHHHHHHHHHHHHHCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHH
KVLERYSLTKEGEEYKKTGLPERQIIDALKNPVSLEELRSCFSPQTVGIATGWLVKKGWA
HHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEEHHHHHHHCHH
KVENGIMVPSGEASEGKDEKALSAFAGKAKTLEELGADEGTVKDLLKRKLVVKHEEKFRT
HHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEE
VSITDAGSELASQGLVLEEEIAQLTPELLKSGAWKEKKFRPYRLDITPKPLYGTKIHPYR
EEEECCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCHHH
RLIEQMRQIFLEMGFTEIKGGIIQSSFWNFDALFQPQDHPARDMQDTFHLGSTCQIPAEY
HHHHHHHHHHHHHCHHHHCCCHHHCCCCCCCEEECCCCCCCHHHHHHHCCCCEEECCCCC
SGKVAAMHENGGDIDSCGWGGIWDRELAGRNVLRTHTTSVTIKYLADNPEPPVKAFCIDR
CCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEECCEEEEEEECCCCCCCHHHHHHHH
AYRRETIDPTHTPEFEQLEGVVMDKDMSFADLLGLLAEFYHRMGFEEVRFRPGYFPYTEP
HHHHCCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
SVEPEVYVDGLGWVELGGAGVFRKEVTEPLGIKAPVLAWGLGVSRLAMLKLGLKDLRLLY
CCCCCEEECCCCEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHH
QSDIDWLRKSEVCKI
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824