Click here to switch to the map view.

The map label for this gene is rtcA

Identifier: 21227399

GI number: 21227399

Start: 1542052

End: 1543110

Strand: Direct

Name: rtcA

Synonym: MM_1297

Alternate gene names: 21227399

Gene position: 1542052-1543110 (Clockwise)

Preceding gene: 21227398

Following gene: 21227401

Centisome position: 37.64

GC content: 47.12

Gene sequence:

>1059_bases
ATGATAGAAATAGACGGTTCTTACGGGGAAGGAGGTGGGCAGTTAGTCAGAACTGCTGTTGCCCTTTCTGCAGTTACAGG
GGAAGAAATAAAAGTTACGAATATAAGAGAAAACAGGCCAAATCCGGGCCTGAAACAGCAACACCTTAAAGCTCTGGAAA
CTGCAGCAAAGATTTGTGGGGCACGGGTTTCAGGGCTTTCTCAGGGCTCCTCGGAATTATTTTTTGCTCCAGTGGAAATA
AAGGGGGGAAAGTATAATATTGATATCGGGACCGCGGGGAGCATAACCTTGCTTTTGCAATGCATTATGCCAGCCCTGCC
TTTTGTAGAAGAAAAGGTAGAGTTAACAATCAGAGGCGGGACTGACGTTGCATGGTCTCCGACAATCGACTACCTGAAGC
ATGTAACCCTCAGGGCACTTGAACAATTTGGATATGCGGGCAGCGTAACCCTGAAAGAGCGCGGCTACTATCCGAGAGGC
GGAGGAAGTGTTTCAGCTGTTTTTGAGCCCTGTAAACTTCATGGTTTTCATTTCAGGAAAACTGAAGGAAATAAAAAAAC
AGAAAGTAACCCGGTGGGAGAAGGTTTGAAGGGAGAAAAAAGCATGATGGAAGAAATTATTGGAGTTTCACACGCATCTA
ACCTTCCTGCACATGTACCGGCCCGCCAGGCTGAATCTGCAAGGTCCCTGTTTCTTGAAGCCGGATATGATGCCCGGATA
GATGTCAGTTCCTCCGAATTTTTCTCCACAGGAAGCGGAATAACTCTCTGGAAGGGCTATTGCGGGGGAAGTGCCCTTGG
AAAAAAGGGGCTGCCTGCTGAAAAAGTAGGCAGGCAAGCTGCGGAAGAAGTTATTAAAGAACTCAGGGCAGGAGCTGCTG
TGGACATTCATCTGGCAGACCAGTTAATTCCCTATATGGCACTTGCTGGCAACAGTTCCTTTACTGTCCGTGAGCTGACC
CTGCATGCTGCGACTAATATCTGGGTGACAGAACAGTTTCTGGATGTGAAGTTCAAAATAAAGGAAAAGGAAGGCCTTTT
TGAAGTATCTGTAGGCTGA

Upstream 100 bases:

>100_bases
AAACTAAAAAAAGGCTGAATTAGAAGCTGAATTAGGAGCTAAATTAGGAGCCGAATGAAAAGCCGAATGAAAAGTTAAGT
GAAATAAACGGGAGTGGAAA

Downstream 100 bases:

>100_bases
TAAAGATAAAAAGCAAAGAAACTGTAAAGAAAAAAGCAGAAAAAATAATACTGAAATACAAAGAAAATGTAAAGGATAGA
AAAATAATACTAAAAGTAAA

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MIEIDGSYGEGGGQLVRTAVALSAVTGEEIKVTNIRENRPNPGLKQQHLKALETAAKICGARVSGLSQGSSELFFAPVEI
KGGKYNIDIGTAGSITLLLQCIMPALPFVEEKVELTIRGGTDVAWSPTIDYLKHVTLRALEQFGYAGSVTLKERGYYPRG
GGSVSAVFEPCKLHGFHFRKTEGNKKTESNPVGEGLKGEKSMMEEIIGVSHASNLPAHVPARQAESARSLFLEAGYDARI
DVSSSEFFSTGSGITLWKGYCGGSALGKKGLPAEKVGRQAAEEVIKELRAGAAVDIHLADQLIPYMALAGNSSFTVRELT
LHAATNIWVTEQFLDVKFKIKEKEGLFEVSVG

Sequences:

>Translated_352_residues
MIEIDGSYGEGGGQLVRTAVALSAVTGEEIKVTNIRENRPNPGLKQQHLKALETAAKICGARVSGLSQGSSELFFAPVEI
KGGKYNIDIGTAGSITLLLQCIMPALPFVEEKVELTIRGGTDVAWSPTIDYLKHVTLRALEQFGYAGSVTLKERGYYPRG
GGSVSAVFEPCKLHGFHFRKTEGNKKTESNPVGEGLKGEKSMMEEIIGVSHASNLPAHVPARQAESARSLFLEAGYDARI
DVSSSEFFSTGSGITLWKGYCGGSALGKKGLPAEKVGRQAAEEVIKELRAGAAVDIHLADQLIPYMALAGNSSFTVRELT
LHAATNIWVTEQFLDVKFKIKEKEGLFEVSVG
>Mature_352_residues
MIEIDGSYGEGGGQLVRTAVALSAVTGEEIKVTNIRENRPNPGLKQQHLKALETAAKICGARVSGLSQGSSELFFAPVEI
KGGKYNIDIGTAGSITLLLQCIMPALPFVEEKVELTIRGGTDVAWSPTIDYLKHVTLRALEQFGYAGSVTLKERGYYPRG
GGSVSAVFEPCKLHGFHFRKTEGNKKTESNPVGEGLKGEKSMMEEIIGVSHASNLPAHVPARQAESARSLFLEAGYDARI
DVSSSEFFSTGSGITLWKGYCGGSALGKKGLPAEKVGRQAAEEVIKELRAGAAVDIHLADQLIPYMALAGNSSFTVRELT
LHAATNIWVTEQFLDVKFKIKEKEGLFEVSVG

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=355, Percent_Identity=36.3380281690141, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI195927044, Length=368, Percent_Identity=35.054347826087, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI157426877, Length=370, Percent_Identity=25.1351351351351, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI48994937, Length=371, Percent_Identity=35.8490566037736, Blast_Score=195, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI32564710, Length=351, Percent_Identity=21.0826210826211, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324564, Length=316, Percent_Identity=22.7848101265823, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI18543269, Length=357, Percent_Identity=33.0532212885154, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI18860065, Length=357, Percent_Identity=21.0084033613445, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_METMA (Q8PXC4)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633321.1
- ProteinModelPortal:   Q8PXC4
- SMR:   Q8PXC4
- GeneID:   1479639
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1297
- NMPDR:   fig|192952.1.peg.1297
- HOGENOM:   HBG683539
- OMA:   EIDGSYG
- ProtClustDB:   PRK04204
- BioCyc:   MMAZ192952:MM1297-MONOMER
- BRENDA:   6.5.1.4
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 37775; Mature: 37775

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 322-322 BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIDGSYGEGGGQLVRTAVALSAVTGEEIKVTNIRENRPNPGLKQQHLKALETAAKICG
CEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
ARVSGLSQGSSELFFAPVEIKGGKYNIDIGTAGSITLLLQCIMPALPFVEEKVELTIRGG
HHHHCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
TDVAWSPTIDYLKHVTLRALEQFGYAGSVTLKERGYYPRGGGSVSAVFEPCKLHGFHFRK
CCCEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHEEECHHHHCCEEEEE
TEGNKKTESNPVGEGLKGEKSMMEEIIGVSHASNLPAHVPARQAESARSLFLEAGYDARI
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCEE
DVSSSEFFSTGSGITLWKGYCGGSALGKKGLPAEKVGRQAAEEVIKELRAGAAVDIHLAD
ECCCCHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHH
QLIPYMALAGNSSFTVRELTLHAATNIWVTEQFLDVKFKIKEKEGLFEVSVG
HHHHHHEECCCCCEEEEEEEEEHHCCEEEEEEEEEEEEEEECCCCCEEEECC
>Mature Secondary Structure
MIEIDGSYGEGGGQLVRTAVALSAVTGEEIKVTNIRENRPNPGLKQQHLKALETAAKICG
CEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
ARVSGLSQGSSELFFAPVEIKGGKYNIDIGTAGSITLLLQCIMPALPFVEEKVELTIRGG
HHHHCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
TDVAWSPTIDYLKHVTLRALEQFGYAGSVTLKERGYYPRGGGSVSAVFEPCKLHGFHFRK
CCCEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHEEECHHHHCCEEEEE
TEGNKKTESNPVGEGLKGEKSMMEEIIGVSHASNLPAHVPARQAESARSLFLEAGYDARI
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCEE
DVSSSEFFSTGSGITLWKGYCGGSALGKKGLPAEKVGRQAAEEVIKELRAGAAVDIHLAD
ECCCCHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHH
QLIPYMALAGNSSFTVRELTLHAATNIWVTEQFLDVKFKIKEKEGLFEVSVG
HHHHHHEECCCCCEEEEEEEEEHHCCEEEEEEEEEEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824