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The map label for this gene is rpa [H]

Identifier: 21227401

GI number: 21227401

Start: 1544636

End: 1546090

Strand: Direct

Name: rpa [H]

Synonym: MM_1299

Alternate gene names: 21227401

Gene position: 1544636-1546090 (Clockwise)

Preceding gene: 21227399

Following gene: 21227403

Centisome position: 37.71

GC content: 41.99

Gene sequence:

>1455_bases
ATGACCGATATTGAAACTATTTATAAGAAGCTCAGCCACGTCATAAGTAAAGAGGATTTCCTGCAGCGCATACAGGAAAA
AGTAGAGAATATGGGAGGGCTCTGTGATGAGACAATGGCTGCCATGCTGGTAGCCAATGAGCTGGGATTCTCGGATGCAG
GCAGAGATAGCATAAAGATAGAAAACATTACCCCCGAAAGCGGACCTGTTAACTTTATTGCAAGAATTATTTCTGTTTTC
GATACCAAAGAATTCACAAGGAATGACGGGACTATAGGAAGGGTAGGAAACCTTATTGTTGGGGATGAGACCGGAAAAGT
AAAGTTAACCCTGTGGGACAATATGGCAGACCTTATTAAAATGGGAAAAATAAAGGCAGGGCAATCCGTTCAGGTCAGTG
GATTCGCAAAGCAGGGATATTCCGGAGTGGAAGTTAATATTGGTAACAATGGAGTCCTGACAGAAAGTGAGGAAGAGATC
GATGTTGTTTCAAATAGTTATAAAATAAAGGATATAAAGGATGGCATGGGAGACATCAACCTTAACGGAAAGGTACTGGA
AGTCTCCGAGATCAGGACATTCCAGAGAAAAGACGGGAACAGCGGGAGGGTAGGAAACCTGATGCTGGGAGACGAAACAG
GTACGCTCAGGGTAACACTCTGGGATGACAAAACTGATTTTTTAAGCCAGGTTGAATATGGGGATAGTATAGAGCTGATT
AATGCTTATGCCCGCGAAAATGCCTTTAGCCAGAAAGTAGAGTTGCAGGTAGGAAACAGGAGTATAATCAGGAAAAGTGA
AAAGAAAATTGAATACGAAGAAGAATTTACTCCTATTGATGATATAAAAGCTGATATGAATAATATAAATATCTCAGGAA
GGATCCTTGATATCTCGGAAGTCAGGACTTTCGAGAAAAAAGATGGATCCACTGGGAGAGTAGGAAATGTCCTTCTGGGC
GATTCTACAGGCAAAATAAGACTGACGCTATGGGATGAAAAAACTGATATTCTTGAAGAAATTGACTTTGATGAAACAGT
TGAAGTCCTTAACGCTTATTCGCGGGAAAATACTTTCAGCCAGCAGGTTGAACTCAACCTGGGAGCCAGAGGAATAATTC
AGAGAAGCGAAAAGAAGGTCGAGTACAGGGAAAAATTTACCGATATAGCCGATATCATCCCCGGAGAAAGTTATTCTATA
CAGGGAAAAGTTGCCGAGATTGGAGAGCTCAGAGAATTTGAAAAAGAAGACGGAACCGAAAATGTTGTTGCAAATCTCCA
GCTTAAAGACGATACAGGCAGCATCCGACTGACACTCTGGGGAGAACAGGCATATGTGATAGAAGACCTTGACATAGATT
CCGAAATCCAGATCATCGATGCTTATGCCAGGTACGGGCTGAACGAAGAAATAGAACTGAGTGTTGGAAACAGGAGTAGA
GTGATTATCCTCTGA

Upstream 100 bases:

>100_bases
TAGACACATATATTGAATAAAAGTACTACCAGAAGTACACCAGTAATTGCAAGTAAGACACCAGTAATTGCAGTAAGCAC
TAACGATTTAAGGGAGCAAC

Downstream 100 bases:

>100_bases
TCATTCCCCTGTTTTCTGTTTATTAAAGCTTTCTCATCACTTTATTTTTTTCCTGCTTACTCACTTTCTGCTTTTATTTT
TTATCTTATTTGTGATCTAT

Product: replication factor A

Products: NA

Alternate protein names: RF-A; RP-A; Replication factor A protein 1; Single-stranded DNA-binding protein; mjaSSB [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTDIETIYKKLSHVISKEDFLQRIQEKVENMGGLCDETMAAMLVANELGFSDAGRDSIKIENITPESGPVNFIARIISVF
DTKEFTRNDGTIGRVGNLIVGDETGKVKLTLWDNMADLIKMGKIKAGQSVQVSGFAKQGYSGVEVNIGNNGVLTESEEEI
DVVSNSYKIKDIKDGMGDINLNGKVLEVSEIRTFQRKDGNSGRVGNLMLGDETGTLRVTLWDDKTDFLSQVEYGDSIELI
NAYARENAFSQKVELQVGNRSIIRKSEKKIEYEEEFTPIDDIKADMNNINISGRILDISEVRTFEKKDGSTGRVGNVLLG
DSTGKIRLTLWDEKTDILEEIDFDETVEVLNAYSRENTFSQQVELNLGARGIIQRSEKKVEYREKFTDIADIIPGESYSI
QGKVAEIGELREFEKEDGTENVVANLQLKDDTGSIRLTLWGEQAYVIEDLDIDSEIQIIDAYARYGLNEEIELSVGNRSR
VIIL

Sequences:

>Translated_484_residues
MTDIETIYKKLSHVISKEDFLQRIQEKVENMGGLCDETMAAMLVANELGFSDAGRDSIKIENITPESGPVNFIARIISVF
DTKEFTRNDGTIGRVGNLIVGDETGKVKLTLWDNMADLIKMGKIKAGQSVQVSGFAKQGYSGVEVNIGNNGVLTESEEEI
DVVSNSYKIKDIKDGMGDINLNGKVLEVSEIRTFQRKDGNSGRVGNLMLGDETGTLRVTLWDDKTDFLSQVEYGDSIELI
NAYARENAFSQKVELQVGNRSIIRKSEKKIEYEEEFTPIDDIKADMNNINISGRILDISEVRTFEKKDGSTGRVGNVLLG
DSTGKIRLTLWDEKTDILEEIDFDETVEVLNAYSRENTFSQQVELNLGARGIIQRSEKKVEYREKFTDIADIIPGESYSI
QGKVAEIGELREFEKEDGTENVVANLQLKDDTGSIRLTLWGEQAYVIEDLDIDSEIQIIDAYARYGLNEEIELSVGNRSR
VIIL
>Mature_483_residues
TDIETIYKKLSHVISKEDFLQRIQEKVENMGGLCDETMAAMLVANELGFSDAGRDSIKIENITPESGPVNFIARIISVFD
TKEFTRNDGTIGRVGNLIVGDETGKVKLTLWDNMADLIKMGKIKAGQSVQVSGFAKQGYSGVEVNIGNNGVLTESEEEID
VVSNSYKIKDIKDGMGDINLNGKVLEVSEIRTFQRKDGNSGRVGNLMLGDETGTLRVTLWDDKTDFLSQVEYGDSIELIN
AYARENAFSQKVELQVGNRSIIRKSEKKIEYEEEFTPIDDIKADMNNINISGRILDISEVRTFEKKDGSTGRVGNVLLGD
STGKIRLTLWDEKTDILEEIDFDETVEVLNAYSRENTFSQQVELNLGARGIIQRSEKKVEYREKFTDIADIIPGESYSIQ
GKVAEIGELREFEKEDGTENVVANLQLKDDTGSIRLTLWGEQAYVIEDLDIDSEIQIIDAYARYGLNEEIELSVGNRSRV
IIL

Specific function: Probably plays an essential for replication of the chromosome, DNA recombination and repair [H]

COG id: COG1599

COG function: function code L; Single-stranded DNA-binding replication protein A (RPA), large (70 kD) subunit and related ssDNA-binding proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 OB DNA-binding domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR013955 [H]

Pfam domain/function: PF08646 Rep_fac-A_C; PF01336 tRNA_anti [H]

EC number: NA

Molecular weight: Translated: 54140; Mature: 54009

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIETIYKKLSHVISKEDFLQRIQEKVENMGGLCDETMAAMLVANELGFSDAGRDSIKI
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
ENITPESGPVNFIARIISVFDTKEFTRNDGTIGRVGNLIVGDETGKVKLTLWDNMADLIK
EECCCCCCHHHHHHHHHHHHCCHHHHCCCCCEECCCCEEEECCCCEEEEEEECCHHHHHH
MGKIKAGQSVQVSGFAKQGYSGVEVNIGNNGVLTESEEEIDVVSNSYKIKDIKDGMGDIN
HCCCCCCCEEEEECCHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEHHCCCCCCC
LNGKVLEVSEIRTFQRKDGNSGRVGNLMLGDETGTLRVTLWDDKTDFLSQVEYGDSIELI
CCCEEEEHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHCCCCCHHHH
NAYARENAFSQKVELQVGNRSIIRKSEKKIEYEEEFTPIDDIKADMNNINISGRILDISE
HHHHHCCCCCEEEEEEECCHHHHHHHHHCCCHHHCCCCHHHHHHHHCCEEEEEEEEEHHH
VRTFEKKDGSTGRVGNVLLGDSTGKIRLTLWDEKTDILEEIDFDETVEVLNAYSRENTFS
HHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHCCCCCC
QQVELNLGARGIIQRSEKKVEYREKFTDIADIIPGESYSIQGKVAEIGELREFEKEDGTE
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCC
NVVANLQLKDDTGSIRLTLWGEQAYVIEDLDIDSEIQIIDAYARYGLNEEIELSVGNRSR
CEEEEEEEECCCCEEEEEEECCCEEEEEECCCCCCEEEEEHHHHCCCCCEEEEEECCCEE
VIIL
EEEC
>Mature Secondary Structure 
TDIETIYKKLSHVISKEDFLQRIQEKVENMGGLCDETMAAMLVANELGFSDAGRDSIKI
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
ENITPESGPVNFIARIISVFDTKEFTRNDGTIGRVGNLIVGDETGKVKLTLWDNMADLIK
EECCCCCCHHHHHHHHHHHHCCHHHHCCCCCEECCCCEEEECCCCEEEEEEECCHHHHHH
MGKIKAGQSVQVSGFAKQGYSGVEVNIGNNGVLTESEEEIDVVSNSYKIKDIKDGMGDIN
HCCCCCCCEEEEECCHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEHHCCCCCCC
LNGKVLEVSEIRTFQRKDGNSGRVGNLMLGDETGTLRVTLWDDKTDFLSQVEYGDSIELI
CCCEEEEHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHCCCCCHHHH
NAYARENAFSQKVELQVGNRSIIRKSEKKIEYEEEFTPIDDIKADMNNINISGRILDISE
HHHHHCCCCCEEEEEEECCHHHHHHHHHCCCHHHCCCCHHHHHHHHCCEEEEEEEEEHHH
VRTFEKKDGSTGRVGNVLLGDSTGKIRLTLWDEKTDILEEIDFDETVEVLNAYSRENTFS
HHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHCCCCCC
QQVELNLGARGIIQRSEKKVEYREKFTDIADIIPGESYSIQGKVAEIGELREFEKEDGTE
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCC
NVVANLQLKDDTGSIRLTLWGEQAYVIEDLDIDSEIQIIDAYARYGLNEEIELSVGNRSR
CEEEEEEEECCCCEEEEEEECCCEEEEEECCCCCCEEEEEHHHHCCCCCEEEEEECCCEE
VIIL
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 9843941 [H]