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The map label for this gene is aroA

Identifier: 21227340

GI number: 21227340

Start: 1465406

End: 1466698

Strand: Direct

Name: aroA

Synonym: MM_1238

Alternate gene names: 21227340

Gene position: 1465406-1466698 (Clockwise)

Preceding gene: 21227339

Following gene: 21227341

Centisome position: 35.77

GC content: 47.64

Gene sequence:

>1293_bases
ATGCGCGCCTCAATTAGCAAATCCTCAATCAAAGGGGAGGTCTTTGCCCCTCCTTCAAAGAGTTACACCCACAGGGCTAT
AACTCTCGCAGCCCTTTCAAAAGAATCGATCATTCACCGTCCCCTCCTTTCCGCTGATACTCTTGCTACAATCAGAGCTT
CTGAGATGTTCGGAGCCGCGGTTAGACGGGAGAAAGAAAATCTCATCATCCAGGGATCTAATGGAAAGCCCGGTATTCCT
GATGATGTAATTGATGCCGCAAATTCAGGGACAACCCTCCGCTTTATGACAGCAATAGCAGGCTTAACTGACGGAATCAC
TGTACTTACAGGAGACTCATCTCTTCGCACGCGTCCAAACGGACCTCTTCTTGAAGTTCTCAACAGGCTGGGAGCAAAAG
CCTGTTCTACGCGAGGAAACGAAAGAGCGCCTATTGTGGTCAAAGGAGGAATTAAGGGATCTGAAGTGGAAATAAGCGGC
TCGATCAGCTCCCAGTTTATCTCTGCTCTTCTTATAGCCTGCCCGCTTGCTGAAAACAGCACCACTCTTTCCATTATAGG
AAAACTGAAGTCAAGACCTTATGTTGACGTGACCATAGAAATGCTCGGGCTGGCAGGAGTCAAAATCCATACAGATGATA
ATAACGGCACGAAATTTATCATCCCCGGAAAACAGAAATACGACCTGAAACAATACACGGTTCCCGGAGACTTTTCTTCT
GCTTCCTACCTGCTAGCAGCTGCAGCCATGCTTGAAGGCTCCGAAATCACAGTCAAAAATCTATTCCCTTCAAAACAGGG
AGATAAAGTGATTATTGATACTCTCAAACAGATGGGAGCAGACATAACATGGGACATGGAAGCTGGCATTGTGACCGTAA
GAGGAGGAAGAAAATTAAAAGCCATTACCTTTGATGCCGGATCAACCCCTGACCTTGTACCGACTGTTGCCGTCCTTGCT
TCAGTTGCCGAAGGGACCAGCAGAATAGAAAACGCCGAGCATGTCCGCTATAAAGAAACAGACCGGCTTCACGCCCTTGC
GACCGAGCTTCCGAAAATGGGAGTCTCCCTCAAAGAAGAAATGGACAGCCTGACAATCACCGGAGGGACTCTTGAGGGAG
CCGAAGTCCACGGCTGGGACGACCACCGGATTGTGATGTCTCTAGCTATAGCAGGCATGGTTGCAGGAAACACGATAGTT
GACACCACTGAGTCTGTATCGATATCCTATCCTGATTTCTTTAAAGATATGCGAAACCTTGGAGCAAAAGTCAAGGAGAT
TCCTGAAGAATAA

Upstream 100 bases:

>100_bases
TTTCGCTCAAACTCAGCAGTCAACTGTTTCAAAAGGTAAAAAGAAAGCAGTCAAACTCAACAACACCCGGATTAAAAAAA
TAGAAGAGCTCTGATTATAC

Downstream 100 bases:

>100_bases
GTGAGATAAAAATTACTCTCCGCTCTGCCAAAGAAATCATTAGCCTTCTAAATGAATCTGAACCAGTTCAGGAATAATAA
GGAATAATAAGAAATGACAT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MRASISKSSIKGEVFAPPSKSYTHRAITLAALSKESIIHRPLLSADTLATIRASEMFGAAVRREKENLIIQGSNGKPGIP
DDVIDAANSGTTLRFMTAIAGLTDGITVLTGDSSLRTRPNGPLLEVLNRLGAKACSTRGNERAPIVVKGGIKGSEVEISG
SISSQFISALLIACPLAENSTTLSIIGKLKSRPYVDVTIEMLGLAGVKIHTDDNNGTKFIIPGKQKYDLKQYTVPGDFSS
ASYLLAAAAMLEGSEITVKNLFPSKQGDKVIIDTLKQMGADITWDMEAGIVTVRGGRKLKAITFDAGSTPDLVPTVAVLA
SVAEGTSRIENAEHVRYKETDRLHALATELPKMGVSLKEEMDSLTITGGTLEGAEVHGWDDHRIVMSLAIAGMVAGNTIV
DTTESVSISYPDFFKDMRNLGAKVKEIPEE

Sequences:

>Translated_430_residues
MRASISKSSIKGEVFAPPSKSYTHRAITLAALSKESIIHRPLLSADTLATIRASEMFGAAVRREKENLIIQGSNGKPGIP
DDVIDAANSGTTLRFMTAIAGLTDGITVLTGDSSLRTRPNGPLLEVLNRLGAKACSTRGNERAPIVVKGGIKGSEVEISG
SISSQFISALLIACPLAENSTTLSIIGKLKSRPYVDVTIEMLGLAGVKIHTDDNNGTKFIIPGKQKYDLKQYTVPGDFSS
ASYLLAAAAMLEGSEITVKNLFPSKQGDKVIIDTLKQMGADITWDMEAGIVTVRGGRKLKAITFDAGSTPDLVPTVAVLA
SVAEGTSRIENAEHVRYKETDRLHALATELPKMGVSLKEEMDSLTITGGTLEGAEVHGWDDHRIVMSLAIAGMVAGNTIV
DTTESVSISYPDFFKDMRNLGAKVKEIPEE
>Mature_430_residues
MRASISKSSIKGEVFAPPSKSYTHRAITLAALSKESIIHRPLLSADTLATIRASEMFGAAVRREKENLIIQGSNGKPGIP
DDVIDAANSGTTLRFMTAIAGLTDGITVLTGDSSLRTRPNGPLLEVLNRLGAKACSTRGNERAPIVVKGGIKGSEVEISG
SISSQFISALLIACPLAENSTTLSIIGKLKSRPYVDVTIEMLGLAGVKIHTDDNNGTKFIIPGKQKYDLKQYTVPGDFSS
ASYLLAAAAMLEGSEITVKNLFPSKQGDKVIIDTLKQMGADITWDMEAGIVTVRGGRKLKAITFDAGSTPDLVPTVAVLA
SVAEGTSRIENAEHVRYKETDRLHALATELPKMGVSLKEEMDSLTITGGTLEGAEVHGWDDHRIVMSLAIAGMVAGNTIV
DTTESVSISYPDFFKDMRNLGAKVKEIPEE

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=333, Percent_Identity=36.6366366366366, Blast_Score=197, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320332, Length=420, Percent_Identity=33.3333333333333, Blast_Score=163, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_METMA (Q8PXI0)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633262.1
- ProteinModelPortal:   Q8PXI0
- SMR:   Q8PXI0
- GeneID:   1479580
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1238
- NMPDR:   fig|192952.1.peg.1238
- HOGENOM:   HBG646626
- OMA:   YTTRYLL
- ProtClustDB:   PRK02427
- BioCyc:   MMAZ192952:MM1238-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 45798; Mature: 45798

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASISKSSIKGEVFAPPSKSYTHRAITLAALSKESIIHRPLLSADTLATIRASEMFGAA
CCCCCCCHHCCCCEECCCCCCCCEEEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHH
VRREKENLIIQGSNGKPGIPDDVIDAANSGTTLRFMTAIAGLTDGITVLTGDSSLRTRPN
HHCCCCCEEEECCCCCCCCCHHHHHCCCCCCEEHHHHHHHCCCCCEEEEECCCCCCCCCC
GPLLEVLNRLGAKACSTRGNERAPIVVKGGIKGSEVEISGSISSQFISALLIACPLAENS
CHHHHHHHHHCHHHHHCCCCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCCCCC
TTLSIIGKLKSRPYVDVTIEMLGLAGVKIHTDDNNGTKFIIPGKQKYDLKQYTVPGDFSS
CEEEEEEHHCCCCEEEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCC
ASYLLAAAAMLEGSEITVKNLFPSKQGDKVIIDTLKQMGADITWDMEAGIVTVRGGRKLK
HHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCEEEEECCCEEE
AITFDAGSTPDLVPTVAVLASVAEGTSRIENAEHVRYKETDRLHALATELPKMGVSLKEE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHCCCHHHHC
MDSLTITGGTLEGAEVHGWDDHRIVMSLAIAGMVAGNTIVDTTESVSISYPDFFKDMRNL
CCCEEEECCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEEECCCCEEECCHHHHHHHHHH
GAKVKEIPEE
CCHHHCCCCC
>Mature Secondary Structure
MRASISKSSIKGEVFAPPSKSYTHRAITLAALSKESIIHRPLLSADTLATIRASEMFGAA
CCCCCCCHHCCCCEECCCCCCCCEEEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHH
VRREKENLIIQGSNGKPGIPDDVIDAANSGTTLRFMTAIAGLTDGITVLTGDSSLRTRPN
HHCCCCCEEEECCCCCCCCCHHHHHCCCCCCEEHHHHHHHCCCCCEEEEECCCCCCCCCC
GPLLEVLNRLGAKACSTRGNERAPIVVKGGIKGSEVEISGSISSQFISALLIACPLAENS
CHHHHHHHHHCHHHHHCCCCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCCCCC
TTLSIIGKLKSRPYVDVTIEMLGLAGVKIHTDDNNGTKFIIPGKQKYDLKQYTVPGDFSS
CEEEEEEHHCCCCEEEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCCC
ASYLLAAAAMLEGSEITVKNLFPSKQGDKVIIDTLKQMGADITWDMEAGIVTVRGGRKLK
HHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCEEEEECCCEEE
AITFDAGSTPDLVPTVAVLASVAEGTSRIENAEHVRYKETDRLHALATELPKMGVSLKEE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHCCCHHHHC
MDSLTITGGTLEGAEVHGWDDHRIVMSLAIAGMVAGNTIVDTTESVSISYPDFFKDMRNL
CCCEEEECCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEEECCCCEEECCHHHHHHHHHH
GAKVKEIPEE
CCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824