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The map label for this gene is thyA [H]

Identifier: 21227339

GI number: 21227339

Start: 1464649

End: 1465302

Strand: Direct

Name: thyA [H]

Synonym: MM_1237

Alternate gene names: 21227339

Gene position: 1464649-1465302 (Clockwise)

Preceding gene: 21227338

Following gene: 21227340

Centisome position: 35.76

GC content: 46.02

Gene sequence:

>654_bases
ATGGAAAACAAGTTTGAGATTGGCAGGATCATCAGGGCAAAGAACATTTCTGATGCATGGTACCGCGGACTTAATATTAT
CTGGAACCACGGGAGAGTAATTACTGACGAAAGGGGAAGCCAGATAAAGGAATTCATGGATTTGATGGTGGTAATTGAAA
ATCCCTATACGGACAGAATTCCCGCAGATACTGCCTGGAATGAAGAAAGGCTTGAAGAGTATGCAAAACAGCTGATCTCA
GGCGAAAATATACAGGACTTTGAGTACACTTACGGGCAGCGTCTCAGGAACTGGAATGGGGAGGTGGACCAGATAGAGTA
TGTAATCGAAAAGCTGAAGGAGAGTCCCACCTCCAGACGTGCTACAGCTGTAACCTGGATTCCGCCTGTGGATACAAAGG
TCAATGAAGTCCCCTGCATGATCCTGGACGACTTCAAGATCCGGGATGAGAAAGTCCACCTTACTACACTTTTCAGGAGC
CACGACTTTGGAGGAGCTTACCCTGCAAACCTTTACGGACTCTCCAAACTTCTTGAATACGTTGCCGAAAAAGTAGGAGT
GGAGCCAGGAGTAATCACCACTGTAAGCATTTCAGCCCATGTCTATGACCACGACTGGGACATGGTAGAAAACATTGTGA
AAGGTATAAACTGA

Upstream 100 bases:

>100_bases
GCTTGATAAAGCAGACAGATTCGGATGAAGAACACTTCTGGTATAACAAATTATTGCACGGTTATTCACACCCTATTCCG
ATCTGATTGGGGAGCTTTTT

Downstream 100 bases:

>100_bases
AAGTTTCGCTCAAACTCAGCAGTCAACTGTTTCAAAAGGTAAAAAGAAAGCAGTCAAACTCAACAACACCCGGATTAAAA
AAATAGAAGAGCTCTGATTA

Product: thymidylate synthase

Products: dihydrofolate; dTMP

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MENKFEIGRIIRAKNISDAWYRGLNIIWNHGRVITDERGSQIKEFMDLMVVIENPYTDRIPADTAWNEERLEEYAKQLIS
GENIQDFEYTYGQRLRNWNGEVDQIEYVIEKLKESPTSRRATAVTWIPPVDTKVNEVPCMILDDFKIRDEKVHLTTLFRS
HDFGGAYPANLYGLSKLLEYVAEKVGVEPGVITTVSISAHVYDHDWDMVENIVKGIN

Sequences:

>Translated_217_residues
MENKFEIGRIIRAKNISDAWYRGLNIIWNHGRVITDERGSQIKEFMDLMVVIENPYTDRIPADTAWNEERLEEYAKQLIS
GENIQDFEYTYGQRLRNWNGEVDQIEYVIEKLKESPTSRRATAVTWIPPVDTKVNEVPCMILDDFKIRDEKVHLTTLFRS
HDFGGAYPANLYGLSKLLEYVAEKVGVEPGVITTVSISAHVYDHDWDMVENIVKGIN
>Mature_217_residues
MENKFEIGRIIRAKNISDAWYRGLNIIWNHGRVITDERGSQIKEFMDLMVVIENPYTDRIPADTAWNEERLEEYAKQLIS
GENIQDFEYTYGQRLRNWNGEVDQIEYVIEKLKESPTSRRATAVTWIPPVDTKVNEVPCMILDDFKIRDEKVHLTTLFRS
HDFGGAYPANLYGLSKLLEYVAEKVGVEPGVITTVSISAHVYDHDWDMVENIVKGIN

Specific function: May catalyze the biosynthesis of dTMP using an unknown cosubstrate [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Archaeal- type thyA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR014620 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: 2.1.1.45

Molecular weight: Translated: 25155; Mature: 25155

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKFEIGRIIRAKNISDAWYRGLNIIWNHGRVITDERGSQIKEFMDLMVVIENPYTDRI
CCCCHHHHHHHHHCCCCHHHHHCCCEEEECCEEEECCCHHHHHHHHHHHHHHCCCCCCCC
PADTAWNEERLEEYAKQLISGENIQDFEYTYGQRLRNWNGEVDQIEYVIEKLKESPTSRR
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
ATAVTWIPPVDTKVNEVPCMILDDFKIRDEKVHLTTLFRSHDFGGAYPANLYGLSKLLEY
CEEEEEECCCCCCCCCCCEEEECCCEECCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHH
VAEKVGVEPGVITTVSISAHVYDHDWDMVENIVKGIN
HHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure
MENKFEIGRIIRAKNISDAWYRGLNIIWNHGRVITDERGSQIKEFMDLMVVIENPYTDRI
CCCCHHHHHHHHHCCCCHHHHHCCCEEEECCEEEECCCHHHHHHHHHHHHHHCCCCCCCC
PADTAWNEERLEEYAKQLISGENIQDFEYTYGQRLRNWNGEVDQIEYVIEKLKESPTSRR
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
ATAVTWIPPVDTKVNEVPCMILDDFKIRDEKVHLTTLFRSHDFGGAYPANLYGLSKLLEY
CEEEEEECCCCCCCCCCCEEEECCCEECCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHH
VAEKVGVEPGVITTVSISAHVYDHDWDMVENIVKGIN
HHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dithiothreitol [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,10-methylenetetrahydrofolate; dUMP

Specific reaction: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

General reaction: Methyl group transfer [C]

Inhibitor: 5'- monophosphate; 10-Formyl dihydrofolyl heptaglutamate; 10-Formyl dihydrofolyl monoglutamate; 10-Formyl tetrahydrofolyl heptaglutamate; 10-Formyl tetrahydrofolyl monoglutamate; Amethopterin; Dithiothreitol [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]