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The map label for this gene is mtnU [H]

Identifier: 21227331

GI number: 21227331

Start: 1456477

End: 1457400

Strand: Reverse

Name: mtnU [H]

Synonym: MM_1229

Alternate gene names: 21227331

Gene position: 1457400-1456477 (Counterclockwise)

Preceding gene: 21227332

Following gene: 21227326

Centisome position: 35.58

GC content: 43.4

Gene sequence:

>924_bases
ATGAGGTACTTAAACAAATTACTTAAACAAAGTACTCAAACACAGTACTTAAACAAAGTACTGAAGTATCTGGTAGATCT
GCATTTACAATTAAAAGGAAGGGAAGTGACCTCTATTATAACCGCCTGTATCCAGATGAATATTTCACCCTGCTCGAAGC
ATGAAAATCTTGATCACGCCCTTTCCCTGGCAGAAGAAGCAGTTTCAAAAGAAGCAGAGTTGCTTGTCTTTCCGGAAGTT
TTCTCAACCGGTTTTTGTTATGAGCGCATAGAAGAAGTGGCTGAAACCGTTTCAGGTCCCACTATTGAGGCTCTCAGTGA
CTTTTCCAGAGAATATGGCTGTATCCTTGCGGGCTCAATGATAGAAAAGAGAGAAATAAAGGATAAGGGGGCAATAAGTC
CGGAAAAAAGAGCTCCTTATCAATATAACCTCGGTTTCTGTATCGAATCCGGAAAACTTGCAGGCATCCGCAGAAAAGTC
CAGCTTTACGGTCCCGAAAAAAAATATTTTGCGTCCGGAGACAGTATAGCCCCTATCAGGCTGCAAAAATACAGCCTCTC
ACTGGGACTTATAGTCTGCAATGAACTCAGGTATCCTGAAGTTGCCCGAAAAATGACTCTTGATGGAGCAGACCTTCTGG
TCTCAGCTGCCGAAATTCCGGATTTTTACATATATCCCTGGCGTATCATGTCAATCTCCAGGGCAATAGAAAATCAACTG
CCGCATATTGCATGTAACAGGGTTGGAAAAGACAGGTATTCTACTTATCCGGGGAGCTCCTTTATTACTGATGGCTGGGG
CCGCATTCTGGCAAAGGCTGGAAAAGAAGAGTGTGTTATTCTGGGGGAAATTGACCTTGACGAGGCAAGAAAAATCAGAC
AAACCGGCTCTATTCTCGAAGACCGCAGGCCTGACCTTTATTAA

Upstream 100 bases:

>100_bases
TTTTGTCCTACCGCCCTGCTCCACTGTCTCATTCTACTGTCATCTCAAATCGTAGTCTTAAACAGGAAAATTGAATATTA
CTGCAGAATAGAATTACCTC

Downstream 100 bases:

>100_bases
CCGTTAAAAAAGTAGTTAAAAAGGTGAACCCTGCCGGGTTACCCTTTAAGTTCCTTTACAATCAGTTCACGGGCTGTCTT
TGCGTCCGCCGTGCCTTTTG

Product: Nitrilase

Products: carboxylate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRYLNKLLKQSTQTQYLNKVLKYLVDLHLQLKGREVTSIITACIQMNISPCSKHENLDHALSLAEEAVSKEAELLVFPEV
FSTGFCYERIEEVAETVSGPTIEALSDFSREYGCILAGSMIEKREIKDKGAISPEKRAPYQYNLGFCIESGKLAGIRRKV
QLYGPEKKYFASGDSIAPIRLQKYSLSLGLIVCNELRYPEVARKMTLDGADLLVSAAEIPDFYIYPWRIMSISRAIENQL
PHIACNRVGKDRYSTYPGSSFITDGWGRILAKAGKEECVILGEIDLDEARKIRQTGSILEDRRPDLY

Sequences:

>Translated_307_residues
MRYLNKLLKQSTQTQYLNKVLKYLVDLHLQLKGREVTSIITACIQMNISPCSKHENLDHALSLAEEAVSKEAELLVFPEV
FSTGFCYERIEEVAETVSGPTIEALSDFSREYGCILAGSMIEKREIKDKGAISPEKRAPYQYNLGFCIESGKLAGIRRKV
QLYGPEKKYFASGDSIAPIRLQKYSLSLGLIVCNELRYPEVARKMTLDGADLLVSAAEIPDFYIYPWRIMSISRAIENQL
PHIACNRVGKDRYSTYPGSSFITDGWGRILAKAGKEECVILGEIDLDEARKIRQTGSILEDRRPDLY
>Mature_307_residues
MRYLNKLLKQSTQTQYLNKVLKYLVDLHLQLKGREVTSIITACIQMNISPCSKHENLDHALSLAEEAVSKEAELLVFPEV
FSTGFCYERIEEVAETVSGPTIEALSDFSREYGCILAGSMIEKREIKDKGAISPEKRAPYQYNLGFCIESGKLAGIRRKV
QLYGPEKKYFASGDSIAPIRLQKYSLSLGLIVCNELRYPEVARKMTLDGADLLVSAAEIPDFYIYPWRIMSISRAIENQL
PHIACNRVGKDRYSTYPGSSFITDGWGRILAKAGKEECVILGEIDLDEARKIRQTGSILEDRRPDLY

Specific function: May be involved in a regulatory step in the methylthioribose (MTR) recycling pathway [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=286, Percent_Identity=29.020979020979, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI297632348, Length=284, Percent_Identity=25.3521126760563, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI5031947, Length=284, Percent_Identity=25.3521126760563, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI297632350, Length=284, Percent_Identity=25.3521126760563, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=286, Percent_Identity=25.5244755244755, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355835, Length=268, Percent_Identity=24.2537313432836, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17933642, Length=308, Percent_Identity=26.2987012987013, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.5.1

Molecular weight: Translated: 34662; Mature: 34662

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLNKLLKQSTQTQYLNKVLKYLVDLHLQLKGREVTSIITACIQMNISPCSKHENLDHA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCCCCHHHH
LSLAEEAVSKEAELLVFPEVFSTGFCYERIEEVAETVSGPTIEALSDFSREYGCILAGSM
HHHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECHH
IEKREIKDKGAISPEKRAPYQYNLGFCIESGKLAGIRRKVQLYGPEKKYFASGDSIAPIR
HHHHHHHHCCCCCCCCCCCEEECCEEEEECCCCCCHHHEEEEECCCHHEECCCCCCCCEE
LQKYSLSLGLIVCNELRYPEVARKMTLDGADLLVSAAEIPDFYIYPWRIMSISRAIENQL
EEEEEEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHCC
PHIACNRVGKDRYSTYPGSSFITDGWGRILAKAGKEECVILGEIDLDEARKIRQTGSILE
CHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHH
DRRPDLY
CCCCCCC
>Mature Secondary Structure
MRYLNKLLKQSTQTQYLNKVLKYLVDLHLQLKGREVTSIITACIQMNISPCSKHENLDHA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCCCCHHHH
LSLAEEAVSKEAELLVFPEVFSTGFCYERIEEVAETVSGPTIEALSDFSREYGCILAGSM
HHHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECHH
IEKREIKDKGAISPEKRAPYQYNLGFCIESGKLAGIRRKVQLYGPEKKYFASGDSIAPIR
HHHHHHHHCCCCCCCCCCCEEECCEEEEECCCCCCHHHEEEEECCCHHEECCCCCCCCEE
LQKYSLSLGLIVCNELRYPEVARKMTLDGADLLVSAAEIPDFYIYPWRIMSISRAIENQL
EEEEEEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHCC
PHIACNRVGKDRYSTYPGSSFITDGWGRILAKAGKEECVILGEIDLDEARKIRQTGSILE
CHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHH
DRRPDLY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrile; H2O

Specific reaction: A nitrile + H2O = a carboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]