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The map label for this gene is pyrI [H]

Identifier: 21227314

GI number: 21227314

Start: 1434747

End: 1435217

Strand: Reverse

Name: pyrI [H]

Synonym: MM_1212

Alternate gene names: 21227314

Gene position: 1435217-1434747 (Counterclockwise)

Preceding gene: 21227315

Following gene: 21227313

Centisome position: 35.04

GC content: 42.68

Gene sequence:

>471_bases
ATGAAGGAAAAAAGAGATTTAAAAGTCCAGGCAATCGAGAGTGGAACTGTGATCGATCACATAAAGTCTGGACAAGCTTT
GAACGTCCTGCGCATTCTGGGAATCTCAAGTGCTTTTCGGGCGACTATCAGTTTTGTGATGAACGCTCCCGGAGCAGGAG
GAAAAAAAGACGTTGTCAAGATTGAGGGCAAAGAACTCAGTGTTGAGGAACTTAACAGGATAGCCCTTATCTCTCCTAAA
GCTACAATCAATATTATAAGGGATTTCGTGGTGGTTCAGAAAAATAACGTCGTGCTCCCGTCTTACGTGGAAGGTGTGGT
CCGCTGTACGAATTCAAGCTGTATATCAAACAGCAGCGAACCTATAAAATCTAAATTCTCGGTAATACAGTCTGAAGAAG
AAGGAGTTTCTCTTCACTGCCTGTACTGCGAGCACGTAATTTCCGAAGAGATTGCAGAAAATTTACTATGA

Upstream 100 bases:

>100_bases
CTGATGTTGATGATACTCCCCATGCCCGTTATTTTGAGCAGGCTTTTTATGGAGTTCCTATCAGGATGGCTTTACTCGCT
CTGACAATAGGGGTGATTGA

Downstream 100 bases:

>100_bases
TTTTTTATCAGCTAAAAGAAAAAAGAGTGCTGAATTTCAAAAAAGCAAATATTCTCAGCACTTTTCTGTATCTAATTTGC
AAGCATCTAATTTGCAGATA

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKEKRDLKVQAIESGTVIDHIKSGQALNVLRILGISSAFRATISFVMNAPGAGGKKDVVKIEGKELSVEELNRIALISPK
ATINIIRDFVVVQKNNVVLPSYVEGVVRCTNSSCISNSSEPIKSKFSVIQSEEEGVSLHCLYCEHVISEEIAENLL

Sequences:

>Translated_156_residues
MKEKRDLKVQAIESGTVIDHIKSGQALNVLRILGISSAFRATISFVMNAPGAGGKKDVVKIEGKELSVEELNRIALISPK
ATINIIRDFVVVQKNNVVLPSYVEGVVRCTNSSCISNSSEPIKSKFSVIQSEEEGVSLHCLYCEHVISEEIAENLL
>Mature_156_residues
MKEKRDLKVQAIESGTVIDHIKSGQALNVLRILGISSAFRATISFVMNAPGAGGKKDVVKIEGKELSVEELNRIALISPK
ATINIIRDFVVVQKNNVVLPSYVEGVVRCTNSSCISNSSEPIKSKFSVIQSEEEGVSLHCLYCEHVISEEIAENLL

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase [H]

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family [H]

Homologues:

Organism=Escherichia coli, GI1790692, Length=152, Percent_Identity=36.1842105263158, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- ProDom:   PD006194 [H]

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C [H]

EC number: 2.1.3.2

Molecular weight: Translated: 17011; Mature: 17011

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKRDLKVQAIESGTVIDHIKSGQALNVLRILGISSAFRATISFVMNAPGAGGKKDVVK
CCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEE
IEGKELSVEELNRIALISPKATINIIRDFVVVQKNNVVLPSYVEGVVRCTNSSCISNSSE
ECCCEECHHHCCEEEEECCCHHHHHHHHHHEECCCCEECHHHHHHHHHHCCCCCCCCCCC
PIKSKFSVIQSEEEGVSLHCLYCEHVISEEIAENLL
HHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEKRDLKVQAIESGTVIDHIKSGQALNVLRILGISSAFRATISFVMNAPGAGGKKDVVK
CCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEE
IEGKELSVEELNRIALISPKATINIIRDFVVVQKNNVVLPSYVEGVVRCTNSSCISNSSE
ECCCEECHHHCCEEEEECCCHHHHHHHHHHEECCCCEECHHHHHHHHHHCCCCCCCCCCC
PIKSKFSVIQSEEEGVSLHCLYCEHVISEEIAENLL
HHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]