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The map label for this gene is pyrB

Identifier: 21227315

GI number: 21227315

Start: 1435214

End: 1436143

Strand: Reverse

Name: pyrB

Synonym: MM_1213

Alternate gene names: 21227315

Gene position: 1436143-1435214 (Counterclockwise)

Preceding gene: 21227316

Following gene: 21227314

Centisome position: 35.06

GC content: 45.59

Gene sequence:

>930_bases
TTGATGTTATTCAAGAACCGGCACGTCATCTCCATGAAAGATTTTTCACGGGAAGAAATCGATTATATTCTGGACATTGC
AGAAAAACTCGAACCTGTAGCCAGGGGGGAAGAAAGGTCCAGGCTTCTTGACGGAAAAATCATTTCTCTTCTTTTTTTTG
AACCAAGTACAAGAACACGACTCTCTTTTGAGGTTGCCACCCGGAGGCTGGGGGGGCAGGTCCTGAGTCTGGGTTCGGTT
GAGGCAAGTTCTGTTATGAAGGGGGAAAATCTTGCTGATACTATCCGCGTGATCAGCAAATATGCAGACCTGATTGTGCT
GCGTCACCCTCTTGACGGGTCAGCAAGAATGGCTACGGAATTTGCAGCTGTCCCTGTGATCAATGGGGGAGATGGTTCTG
TCCACCATCCTACCCAGACTTTTCTTGACCTCTATACCATCCGCCGAGAAAGCCATCTTGAAGGCTTGAAAATTGCCATG
GCAGGAGACCTGAAATATGGGAGAACAGTACATTCCCTCTGCTATGCCCTTTCCCTGTATGGGGCAGAAATGACTTTTGT
TTCACCTCCGGAACTCAGGATGCCCCGGGAAATTGTCCGAGAACTTCGGAAGAAAAATATCCGCGTAAAGGAAAGCGAGT
GCCTTGAGGAAATAATAGGAGATATTGAAGTTCTTTACATGACAAGAGTTCAGAGGGAGCGTTTTCCTGACCCTCAAGAA
TACGAAAAGGTTAAGAATAAGTTAAAAATTACGGGAGATCTCTTAAAATCCGCAGATCCCGAACTTAAAATCCTTCACCC
TCTTCCAAGGGTAAATGAAATATCTCCTGATGTTGATGATACTCCCCATGCCCGTTATTTTGAGCAGGCTTTTTATGGAG
TTCCTATCAGGATGGCTTTACTCGCTCTGACAATAGGGGTGATTGAATGA

Upstream 100 bases:

>100_bases
AACCGCGCAGCACCGAGTCTAATCAGGGATTAATTGCTGCAGTATTCCATGGGAAATTTTCAGAGCAAAGATTATATCCC
TGTGCACCATTTATGAAAAA

Downstream 100 bases:

>100_bases
AGGAAAAAAGAGATTTAAAAGTCCAGGCAATCGAGAGTGGAACTGTGATCGATCACATAAAGTCTGGACAAGCTTTGAAC
GTCCTGCGCATTCTGGGAAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MMLFKNRHVISMKDFSREEIDYILDIAEKLEPVARGEERSRLLDGKIISLLFFEPSTRTRLSFEVATRRLGGQVLSLGSV
EASSVMKGENLADTIRVISKYADLIVLRHPLDGSARMATEFAAVPVINGGDGSVHHPTQTFLDLYTIRRESHLEGLKIAM
AGDLKYGRTVHSLCYALSLYGAEMTFVSPPELRMPREIVRELRKKNIRVKESECLEEIIGDIEVLYMTRVQRERFPDPQE
YEKVKNKLKITGDLLKSADPELKILHPLPRVNEISPDVDDTPHARYFEQAFYGVPIRMALLALTIGVIE

Sequences:

>Translated_309_residues
MMLFKNRHVISMKDFSREEIDYILDIAEKLEPVARGEERSRLLDGKIISLLFFEPSTRTRLSFEVATRRLGGQVLSLGSV
EASSVMKGENLADTIRVISKYADLIVLRHPLDGSARMATEFAAVPVINGGDGSVHHPTQTFLDLYTIRRESHLEGLKIAM
AGDLKYGRTVHSLCYALSLYGAEMTFVSPPELRMPREIVRELRKKNIRVKESECLEEIIGDIEVLYMTRVQRERFPDPQE
YEKVKNKLKITGDLLKSADPELKILHPLPRVNEISPDVDDTPHARYFEQAFYGVPIRMALLALTIGVIE
>Mature_309_residues
MMLFKNRHVISMKDFSREEIDYILDIAEKLEPVARGEERSRLLDGKIISLLFFEPSTRTRLSFEVATRRLGGQVLSLGSV
EASSVMKGENLADTIRVISKYADLIVLRHPLDGSARMATEFAAVPVINGGDGSVHHPTQTFLDLYTIRRESHLEGLKIAM
AGDLKYGRTVHSLCYALSLYGAEMTFVSPPELRMPREIVRELRKKNIRVKESECLEEIIGDIEVLYMTRVQRERFPDPQE
YEKVKNKLKITGDLLKSADPELKILHPLPRVNEISPDVDDTPHARYFEQAFYGVPIRMALLALTIGVIE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=302, Percent_Identity=44.0397350993377, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI38788445, Length=296, Percent_Identity=26.3513513513513, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=49.3421052631579, Blast_Score=286, Evalue=9e-79,
Organism=Escherichia coli, GI1790703, Length=291, Percent_Identity=30.2405498281787, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786469, Length=284, Percent_Identity=27.112676056338, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=319, Percent_Identity=42.0062695924765, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=46.078431372549, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322373, Length=307, Percent_Identity=27.6872964169381, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24642586, Length=304, Percent_Identity=44.7368421052632, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_METMA (Q8PXK5)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633237.1
- ProteinModelPortal:   Q8PXK5
- SMR:   Q8PXK5
- GeneID:   1479555
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1213
- NMPDR:   fig|192952.1.peg.1213
- HOGENOM:   HBG579429
- OMA:   AQYIDSH
- ProtClustDB:   PRK00856
- BioCyc:   MMAZ192952:MM1213-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35096; Mature: 35096

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLFKNRHVISMKDFSREEIDYILDIAEKLEPVARGEERSRLLDGKIISLLFFEPSTRTR
CCEECCCCEEEHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCCE
LSFEVATRRLGGQVLSLGSVEASSVMKGENLADTIRVISKYADLIVLRHPLDGSARMATE
EHHHHHHHHHCCCEEECCCCCHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHH
FAAVPVINGGDGSVHHPTQTFLDLYTIRRESHLEGLKIAMAGDLKYGRTVHSLCYALSLY
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
GAEMTFVSPPELRMPREIVRELRKKNIRVKESECLEEIIGDIEVLYMTRVQRERFPDPQE
CCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHH
YEKVKNKLKITGDLLKSADPELKILHPLPRVNEISPDVDDTPHARYFEQAFYGVPIRMAL
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
LALTIGVIE
HHHHHHHCC
>Mature Secondary Structure
MMLFKNRHVISMKDFSREEIDYILDIAEKLEPVARGEERSRLLDGKIISLLFFEPSTRTR
CCEECCCCEEEHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCCE
LSFEVATRRLGGQVLSLGSVEASSVMKGENLADTIRVISKYADLIVLRHPLDGSARMATE
EHHHHHHHHHCCCEEECCCCCHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHHH
FAAVPVINGGDGSVHHPTQTFLDLYTIRRESHLEGLKIAMAGDLKYGRTVHSLCYALSLY
HHEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
GAEMTFVSPPELRMPREIVRELRKKNIRVKESECLEEIIGDIEVLYMTRVQRERFPDPQE
CCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHH
YEKVKNKLKITGDLLKSADPELKILHPLPRVNEISPDVDDTPHARYFEQAFYGVPIRMAL
HHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
LALTIGVIE
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824