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The map label for this gene is spsK [H]

Identifier: 21227270

GI number: 21227270

Start: 1387096

End: 1387905

Strand: Direct

Name: spsK [H]

Synonym: MM_1168

Alternate gene names: 21227270

Gene position: 1387096-1387905 (Clockwise)

Preceding gene: 21227269

Following gene: 21227271

Centisome position: 33.86

GC content: 42.59

Gene sequence:

>810_bases
ATGGTGGTGGGAACCATTAAAACGCTGATCATCGGCTCCAGCGGGATGCTCGGGTCAGACCTGTGCAAAGTCTTTCCCGA
TGCTGTTAAACTGACACACCATGACCTCGACATCACAGACAGAGAGCAGGTTATTGAATCCATTTTAAAAATAAAACCTG
ATGTTGTAATAAACGCAGCAGCCTACACCAATGTGGACGGCTGTGAAGACAACAAGGAACTTGCGTTCCAGGTCAACGGA
TCCGGACCCGGATACATTGCAGAAGCCTGCGCCAGAGCAGGTGCAAAACTCGTTCATTTCAGTACTGATTATGTTTTTGA
CGGCTCCAAAAAAGAGTATGTGGAGTCTGACATCCCGGATCCGATTAATGTATACGGTGATTCAAAACTTCTCGGTGAAA
AGAAGATCATCGAGAATATGGATGATTACAGGATTGTAAGAATTTCCTGGCTTTTTGGAATACATGGGAAAAATTTCGTT
GAAACCATGCTGAAACTATCAGGAGAAATGGATACCGTAAAGGTTGTCAATGACCAGTTCGGCAAACCCACCTACACAAT
GGACCTTGCCAGAAAAGTAAAGGAAATAATTGAACTCGAACCCGGAATTTATCATATTACAAATGATGGAATCTGTTCCT
GGTACGAATTTGCCTCTTCTATTATCGATAATGTTATTCCGTGCACCAGTGAAGAATTTCAAAGGAAAGCAAAACGTCCA
ATGTATTCAGTTCTTGTGAATACTAAAACCGGACCCATGAGACACTGGAGAGAAGCACTTAAGGACTATTTGCAGGGGAG
AAATATATGA

Upstream 100 bases:

>100_bases
ACTCCCTTGACGGCAGCAAACTGGAAAAAATGGGCTGGAAACCCAGATATGATTTTGATACCGCTCTCGAAAAGACTGTC
AGCTGGTATGTAGAAAATAA

Downstream 100 bases:

>100_bases
AAGAGGTTATACTTGCAGGCGGTACGGGCAGCAGACTGTATCCTCTAACCAAAGTAACTAACAAGCACCTTTTGCCTGTC
TATGACAAGCCAATGATCTA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MVVGTIKTLIIGSSGMLGSDLCKVFPDAVKLTHHDLDITDREQVIESILKIKPDVVINAAAYTNVDGCEDNKELAFQVNG
SGPGYIAEACARAGAKLVHFSTDYVFDGSKKEYVESDIPDPINVYGDSKLLGEKKIIENMDDYRIVRISWLFGIHGKNFV
ETMLKLSGEMDTVKVVNDQFGKPTYTMDLARKVKEIIELEPGIYHITNDGICSWYEFASSIIDNVIPCTSEEFQRKAKRP
MYSVLVNTKTGPMRHWREALKDYLQGRNI

Sequences:

>Translated_269_residues
MVVGTIKTLIIGSSGMLGSDLCKVFPDAVKLTHHDLDITDREQVIESILKIKPDVVINAAAYTNVDGCEDNKELAFQVNG
SGPGYIAEACARAGAKLVHFSTDYVFDGSKKEYVESDIPDPINVYGDSKLLGEKKIIENMDDYRIVRISWLFGIHGKNFV
ETMLKLSGEMDTVKVVNDQFGKPTYTMDLARKVKEIIELEPGIYHITNDGICSWYEFASSIIDNVIPCTSEEFQRKAKRP
MYSVLVNTKTGPMRHWREALKDYLQGRNI
>Mature_269_residues
MVVGTIKTLIIGSSGMLGSDLCKVFPDAVKLTHHDLDITDREQVIESILKIKPDVVINAAAYTNVDGCEDNKELAFQVNG
SGPGYIAEACARAGAKLVHFSTDYVFDGSKKEYVESDIPDPINVYGDSKLLGEKKIIENMDDYRIVRISWLFGIHGKNFV
ETMLKLSGEMDTVKVVNDQFGKPTYTMDLARKVKEIIELEPGIYHITNDGICSWYEFASSIIDNVIPCTSEEFQRKAKRP
MYSVLVNTKTGPMRHWREALKDYLQGRNI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=238, Percent_Identity=29.8319327731092, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI11034825, Length=238, Percent_Identity=29.8319327731092, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1788352, Length=265, Percent_Identity=33.5849056603774, Blast_Score=157, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 30180; Mature: 30180

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGTIKTLIIGSSGMLGSDLCKVFPDAVKLTHHDLDITDREQVIESILKIKPDVVINAA
CCEEEEEEEEECCCCCCHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEH
AYTNVDGCEDNKELAFQVNGSGPGYIAEACARAGAKLVHFSTDYVFDGSKKEYVESDIPD
HCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCEEECCCHHHHHHCCCCC
PINVYGDSKLLGEKKIIENMDDYRIVRISWLFGIHGKNFVETMLKLSGEMDTVKVVNDQF
CCEECCCCHHHCHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEHHHCC
GKPTYTMDLARKVKEIIELEPGIYHITNDGICSWYEFASSIIDNVIPCTSEEFQRKAKRP
CCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
MYSVLVNTKTGPMRHWREALKDYLQGRNI
EEEEEEECCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVVGTIKTLIIGSSGMLGSDLCKVFPDAVKLTHHDLDITDREQVIESILKIKPDVVINAA
CCEEEEEEEEECCCCCCHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEH
AYTNVDGCEDNKELAFQVNGSGPGYIAEACARAGAKLVHFSTDYVFDGSKKEYVESDIPD
HCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCEEECCCHHHHHHCCCCC
PINVYGDSKLLGEKKIIENMDDYRIVRISWLFGIHGKNFVETMLKLSGEMDTVKVVNDQF
CCEECCCCHHHCHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEHHHCC
GKPTYTMDLARKVKEIIELEPGIYHITNDGICSWYEFASSIIDNVIPCTSEEFQRKAKRP
CCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
MYSVLVNTKTGPMRHWREALKDYLQGRNI
EEEEEEECCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]