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The map label for this gene is GloA3 [H]

Identifier: 20808750

GI number: 20808750

Start: 2274143

End: 2274544

Strand: Reverse

Name: GloA3 [H]

Synonym: TTE2386

Alternate gene names: 20808750

Gene position: 2274544-2274143 (Counterclockwise)

Preceding gene: 20808751

Following gene: 20808749

Centisome position: 84.57

GC content: 44.53

Gene sequence:

>402_bases
GTGATTAAAAAGGTAGACCATATAGGGATAGCAGTAAAGAGCATAGAAGAAGCGAGAAAGTTTTATGAAGAAGTGCTGGG
CTTGAAAGTGACAGGGATAGAAGAAGTTAAGGAGCAGAAGGTAAAGACGGCCTTTATTCCAGTTGGGGAGAGCGAGATAG
AGCTTTTAGAGTCCACATCAGAGGATGGGCCTGTCGCTAAATTTATAGAAAAAAGAGGGGAAGGCATACACCACATAGCA
CTTCAGGTAGATGACATTGAAAAGGCGTTAGAGGAGTTGAAGGGAAAAGGAGTAAAGCTCATAGATGAAGTGCCAAGGTA
TGGGGCAGGAGGGGCGAAAATTGCCTTTGTCCACCCAAAGAGCGCAAATGGAGTATTGTTGGAGCTTTGTGAGAGGAAGT
GA

Upstream 100 bases:

>100_bases
GAATTTGACCAGAAAATTCAAAAAGTCATGGCTAAAGAAATTGACCCTTATACAGTTGCAGAAGCTCTCTATAGGCAAAT
ACTTGAGAGGAGGGAATGAT

Downstream 100 bases:

>100_bases
AAGTTGTTGAGCTTTTTCTTCTAGTATGTTATATTATAGTAGGTACTAATAATAACTGCTACTAAAACTTTATTTTATTA
AGGAGGCGTCTTGATGAGCG

Product: lactoylglutathione lyase and related lyase

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGESEIELLESTSEDGPVAKFIEKRGEGIHHIA
LQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPKSANGVLLELCERK

Sequences:

>Translated_133_residues
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGESEIELLESTSEDGPVAKFIEKRGEGIHHIA
LQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPKSANGVLLELCERK
>Mature_133_residues
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGESEIELLESTSEDGPVAKFIEKRGEGIHHIA
LQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPKSANGVLLELCERK

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI188035928, Length=127, Percent_Identity=37.007874015748, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17506387, Length=130, Percent_Identity=36.9230769230769, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR018146
- InterPro:   IPR017515 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14632; Mature: 14632

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGESEIELLESTS
CCCCCHHHHHHHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHHEEECCCCHHHHHHHCCC
EDGPVAKFIEKRGEGIHHIALQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPK
CCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEECCC
SANGVLLELCERK
CCCCHHHHHHCCC
>Mature Secondary Structure
MIKKVDHIGIAVKSIEEARKFYEEVLGLKVTGIEEVKEQKVKTAFIPVGESEIELLESTS
CCCCCHHHHHHHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHHEEECCCCHHHHHHHCCC
EDGPVAKFIEKRGEGIHHIALQVDDIEKALEELKGKGVKLIDEVPRYGAGGAKIAFVHPK
CCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEECCC
SANGVLLELCERK
CCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]