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The map label for this gene is PtsA [H]

Identifier: 20808700

GI number: 20808700

Start: 2216928

End: 2218649

Strand: Reverse

Name: PtsA [H]

Synonym: TTE2334

Alternate gene names: 20808700

Gene position: 2218649-2216928 (Counterclockwise)

Preceding gene: 20808701

Following gene: 20808699

Centisome position: 82.49

GC content: 40.24

Gene sequence:

>1722_bases
ATGTTAAAAGGAGTTGCGGCATCGCCTGGAATAGCTATAGGCAAAGCTTTTTTGTATACCAAAGAAAAAGTGACAATAAA
TGTAGAAAAAATTGAAGAATCGAAAGTGGAAGAGGAAATTGCGAAATTTAGAAAGGCTTTGGAAGTTACACAGGAGGAGA
TAGAGAAAATCAAGGAAAAAGCTTTGAAAGAATTTGGAAAAGAAAAAGCAGAAATTTTTGAAGCACATTTGATGCTGGCA
AGTGACCCCGAACTTATTGAAGGGGTAGAAAACATGATTAAAACAGAACTTGTAACAGCTGATAACGCAGTTAATAAGGT
TATAGAGCAAAATGCTTCTGTGATGGAAAGTTTGAATGACGAATATTTGAAAGAGAGAGCTGTTGATTTAAGGGATGTGG
GCAATCGCATAATAGAAAATTTATTGGGGGTAAAAAGTGTCAATCTTTCTGACCTTGAAGAAGAGGTTGTGGTCATTGCG
AGAGACCTTACTCCTTCAGATACAGCTACTATGAAGAAAGAAATGGTTTTAGGGTTTGCTACAGACGTGGGAGGAAGGAC
TTCACATACAGCTATAATGGCTCGCTCTTTGGAAATCCCTGCAGTTGTGGGCTTAGGCAATGTGACTTCTCAGGTCAAAG
CCGGGGACCTCGTTATAGTAGACGGATTAGAAGGGATTGTAATTGTAAATCCTGATGAAAAGACTGTAGAGGACTACAAA
AGCAAAAAAGAGAGCTATGAGAAAAAGGTGGAAGGATTAAAGCAGTTAAAAGATTTGCCTGCAGAAACACCCGATGGAAA
GAAAGTCATGTTGGCAGCAAATATAGGGACTCCCAAAGATGTAGCCTCAGCGTTAGCTAATGGAGCAGAGGGAGTAGGGC
TTTTTAGGACAGAATTTTTGTACATGGACAGAAATAGCCTCCCTAGTGAAGAAGAGCAATTTGAAGCTTATAAGGAAGTT
GTAGAAAAAATGGGAGGGAGGCCTGTCACTATAAGGACACTGGACATAGGAGGAGACAAAGAGCTTCCTTACCTTGATAT
GCCAAAAGAGATGAATCCTTTTTTGGGATACAGGGCTATAAGGCTTTGTTTGGATAGACCGGATATTTTCAAGACTCAAT
TAAGGGCTATTTTAAGAGCAAGCGCTTACGGAAATGTACAGATAATGTATCCCATGATTTCGTCTGTAGAGGAAGTGAGA
AAAGCGAACTCTATTTTAGAAGAGGTAAAAGCTGAGCTTGATAGAGAAGGAGTAAAATACGATAAGGAGATAAAAGTGGG
CATAATGGTAGAAATTCCTTCTGCGGCTGTGACGGCAGATATTCTTGCTAAGGAGGTAGATTTCTTTAGCATAGGGACTA
ATGACCTGACGCAGTATACGCTTGCGGTAGACAGGATGAACGAACATGTAAAAGAATACTATCAGCCCTTCCATCCGGCT
ATTTTGCGACTTGTAAAAATGGTGATTGACGCTGCCCATAAAGAAGGGAAGTTTGCCGCTATGTGCGGGGAAATGGCAGG
AGACCCTCTTGCGGCTGTCATTCTTTTGGGATTAGGCCTTGATGAATTTTCCATGAGCGCAACTTCCATACCGGAGATTA
AGAACATAATAAGAAATGTGGAGTATGAAAAAGCGAAAGAAATTGCAGAAAAAGCCTTAAACATGTCGGAAGCGAGAGAG
ATAGAAAAAATGATGAAGGACGTGATAAAAGATATTGGCTAA

Upstream 100 bases:

>100_bases
TTCTTGGCTTTTTTATTTTACCTTTGTGCAAAAGTTTCGCATTGAAGTGAAAACTGTGCTATAATAAACCTTAGAACTAA
TTTCGGGGAGGTTTTTTCAA

Downstream 100 bases:

>100_bases
GTCTCTGGCTCTGCCAGAGATTTTTTATTAAAAAATTATCAAAAAAATAAAGGAAAATCTCAAAATGTATAGAATAATAA
ATATTAAAGACTATTTGCTA

Product: phosphoenolpyruvate-protein kinase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MLKGVAASPGIAIGKAFLYTKEKVTINVEKIEESKVEEEIAKFRKALEVTQEEIEKIKEKALKEFGKEKAEIFEAHLMLA
SDPELIEGVENMIKTELVTADNAVNKVIEQNASVMESLNDEYLKERAVDLRDVGNRIIENLLGVKSVNLSDLEEEVVVIA
RDLTPSDTATMKKEMVLGFATDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVKAGDLVIVDGLEGIVIVNPDEKTVEDYK
SKKESYEKKVEGLKQLKDLPAETPDGKKVMLAANIGTPKDVASALANGAEGVGLFRTEFLYMDRNSLPSEEEQFEAYKEV
VEKMGGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAIRLCLDRPDIFKTQLRAILRASAYGNVQIMYPMISSVEEVR
KANSILEEVKAELDREGVKYDKEIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKEYYQPFHPA
ILRLVKMVIDAAHKEGKFAAMCGEMAGDPLAAVILLGLGLDEFSMSATSIPEIKNIIRNVEYEKAKEIAEKALNMSEARE
IEKMMKDVIKDIG

Sequences:

>Translated_573_residues
MLKGVAASPGIAIGKAFLYTKEKVTINVEKIEESKVEEEIAKFRKALEVTQEEIEKIKEKALKEFGKEKAEIFEAHLMLA
SDPELIEGVENMIKTELVTADNAVNKVIEQNASVMESLNDEYLKERAVDLRDVGNRIIENLLGVKSVNLSDLEEEVVVIA
RDLTPSDTATMKKEMVLGFATDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVKAGDLVIVDGLEGIVIVNPDEKTVEDYK
SKKESYEKKVEGLKQLKDLPAETPDGKKVMLAANIGTPKDVASALANGAEGVGLFRTEFLYMDRNSLPSEEEQFEAYKEV
VEKMGGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAIRLCLDRPDIFKTQLRAILRASAYGNVQIMYPMISSVEEVR
KANSILEEVKAELDREGVKYDKEIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKEYYQPFHPA
ILRLVKMVIDAAHKEGKFAAMCGEMAGDPLAAVILLGLGLDEFSMSATSIPEIKNIIRNVEYEKAKEIAEKALNMSEARE
IEKMMKDVIKDIG
>Mature_573_residues
MLKGVAASPGIAIGKAFLYTKEKVTINVEKIEESKVEEEIAKFRKALEVTQEEIEKIKEKALKEFGKEKAEIFEAHLMLA
SDPELIEGVENMIKTELVTADNAVNKVIEQNASVMESLNDEYLKERAVDLRDVGNRIIENLLGVKSVNLSDLEEEVVVIA
RDLTPSDTATMKKEMVLGFATDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVKAGDLVIVDGLEGIVIVNPDEKTVEDYK
SKKESYEKKVEGLKQLKDLPAETPDGKKVMLAANIGTPKDVASALANGAEGVGLFRTEFLYMDRNSLPSEEEQFEAYKEV
VEKMGGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAIRLCLDRPDIFKTQLRAILRASAYGNVQIMYPMISSVEEVR
KANSILEEVKAELDREGVKYDKEIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKEYYQPFHPA
ILRLVKMVIDAAHKEGKFAAMCGEMAGDPLAAVILLGLGLDEFSMSATSIPEIKNIIRNVEYEKAKEIAEKALNMSEARE
IEKMMKDVIKDIG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=54.1155866900175, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1788726, Length=570, Percent_Identity=36.6666666666667, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI48994992, Length=516, Percent_Identity=38.3720930232558, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1789193, Length=563, Percent_Identity=35.3463587921847, Blast_Score=331, Evalue=8e-92,
Organism=Escherichia coli, GI1787994, Length=553, Percent_Identity=28.2097649186257, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI226510935, Length=230, Percent_Identity=26.9565217391304, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63458; Mature: 63458

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGVAASPGIAIGKAFLYTKEKVTINVEKIEESKVEEEIAKFRKALEVTQEEIEKIKEK
CCCCCCCCCCHHHHHHHEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKEFGKEKAEIFEAHLMLASDPELIEGVENMIKTELVTADNAVNKVIEQNASVMESLND
HHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLKERAVDLRDVGNRIIENLLGVKSVNLSDLEEEVVVIARDLTPSDTATMKKEMVLGFA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHH
TDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVKAGDLVIVDGLEGIVIVNPDEKTVEDYK
HCCCCCCHHHHHHHHCCCCCEEEECCCHHHHCCCCCEEEEECCCEEEEECCCHHHHHHHH
SKKESYEKKVEGLKQLKDLPAETPDGKKVMLAANIGTPKDVASALANGAEGVGLFRTEFL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHEE
YMDRNSLPSEEEQFEAYKEVVEKMGGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAI
EECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
RLCLDRPDIFKTQLRAILRASAYGNVQIMYPMISSVEEVRKANSILEEVKAELDREGVKY
HHHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DKEIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKEYYQPFHPA
CCEEEEEEEEECCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
ILRLVKMVIDAAHKEGKFAAMCGEMAGDPLAAVILLGLGLDEFSMSATSIPEIKNIIRNV
HHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCC
EYEKAKEIAEKALNMSEAREIEKMMKDVIKDIG
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKGVAASPGIAIGKAFLYTKEKVTINVEKIEESKVEEEIAKFRKALEVTQEEIEKIKEK
CCCCCCCCCCHHHHHHHEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKEFGKEKAEIFEAHLMLASDPELIEGVENMIKTELVTADNAVNKVIEQNASVMESLND
HHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLKERAVDLRDVGNRIIENLLGVKSVNLSDLEEEVVVIARDLTPSDTATMKKEMVLGFA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHH
TDVGGRTSHTAIMARSLEIPAVVGLGNVTSQVKAGDLVIVDGLEGIVIVNPDEKTVEDYK
HCCCCCCHHHHHHHHCCCCCEEEECCCHHHHCCCCCEEEEECCCEEEEECCCHHHHHHHH
SKKESYEKKVEGLKQLKDLPAETPDGKKVMLAANIGTPKDVASALANGAEGVGLFRTEFL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHEE
YMDRNSLPSEEEQFEAYKEVVEKMGGRPVTIRTLDIGGDKELPYLDMPKEMNPFLGYRAI
EECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
RLCLDRPDIFKTQLRAILRASAYGNVQIMYPMISSVEEVRKANSILEEVKAELDREGVKY
HHHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DKEIKVGIMVEIPSAAVTADILAKEVDFFSIGTNDLTQYTLAVDRMNEHVKEYYQPFHPA
CCEEEEEEEEECCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
ILRLVKMVIDAAHKEGKFAAMCGEMAGDPLAAVILLGLGLDEFSMSATSIPEIKNIIRNV
HHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCC
EYEKAKEIAEKALNMSEAREIEKMMKDVIKDIG
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]